Commercial Latex Agglutination Kits for the Detection of Food borne Salmonella

1991 ◽  
Vol 54 (9) ◽  
pp. 725-730 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
A. M. SEWELL ◽  
P. GRECO
Keyword(s):  
Food Samples ◽  
Latex Agglutination ◽  
Clinical Samples ◽  
Salmonella Spp ◽  
Concurrent Use ◽  
Food Borne ◽  
Analytical Test ◽  
Contaminated Food ◽  
Pure Cultures ◽  
Vi Antigen

The ability of the Bactigen® Salmonella Shigella (BSST), the Microscreen® (MS), and the Spectate® (SPECT) latex agglutination kits to detect Salmonella in pure cultures and in naturally contaminated foods was examined. Of 190 Salmonella strains tested, the MS, BSST, and SPECT systems correctly identified 89.5, 81.6, and 66.3% of the test cultures, respectively. The sensitivity of SPECT increased to 92.7% when only strains belonging to the targeted serogroups (somatic A to E plus G) and strains harboring the Vi antigen were considered. The lack of specificity of the MS (3.4%), SPECT (17.0%), and BSST (33.9%) systems with 59 cultures of nonsalmonellae varied widely, with Citrobacter freundii and Escherichia coli accounting for many of the false-positive reactions. Examination of foods according to the prescribed MS and SPECT analytical test protocols identified respectively, 18 (75%) and 19 (79.2%) of the 24 food samples found to contain Salmonella spp. by a standard cultural method. Although instructions with the BSST kit indicate that the product is intended for the analysis of clinical samples, the system nevertheless identified 21 (87.5%) contaminated food samples under homologous MS and SPECT test conditions. The concurrent use of TBG43 with enrichment media recommended by kit manufactures enhanced the sensitivities of MS (83.3%), SPECT (91.7%), and BSST (91.7%). Attempts to effect greater method brevity through the application of latex kits at various stages of the standard cultural procedure were counterproductive.

scholarly journals Listeria monocytogenes: An emerging food-borne pathogen and its public health implications

2016 ◽  
Vol 10 (02) ◽  
pp. 149-154 ◽  
Author(s):  
Waffa W Reda ◽  
Khaled Abdel-Moein ◽  
Ahmed Hegazi ◽  
Yasmin Mohamed ◽  
Khaled Abdel-Razik
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Food Samples ◽  
Food Borne ◽  
Contaminated Food ◽  
Luncheon Meat ◽  
Stool Specimens ◽  
Polymerase Chain ◽  
Minced Meat

Introduction: Listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. The aim of the present study was to demonstrate the importance of L. monocytogenes as a food-borne pathogen. Methodology: A total of 340 samples were collected from different localities in El Giza Governorate, Egypt, to check the occurrence of L. monocytogenes in that area. The collected samples comprised 250 food samples, 40 swabs from food refrigerators, and 50 stool specimens from diarrheic children. L. monocytogenes was isolated from the examined samples according to the International Organization for Standardization. The isolates were tested biochemically using Listeria Microbact 12L and confirmed by polymerase chain reaction. Results: The isolation rates of L. monocytogenes were 8% in beef burger, 4% in minced meat, 4% in luncheon meat, while sausage samples were all negative. Eight percent of raw milk samples were positive for L. monocytogenes, whereas cheese samples and refrigerator swabs were negative. Only Listeria grayi was isolated from human stools (2.5%). Conclusion: The high isolation rates of L. monocytogenes among the examined food stuffs highlight the crucial role of food as an important vehicle for this pathogen. More efforts should be made to ensure safe handling and processing of these foods to reduce the transmission of L. monocytogenes to humans.

AnDiaTec Salmonella sp. PCR-ELISA for Analysis of Food Samples

2004 ◽  
Vol 67 (8) ◽  
pp. 1585-1590 ◽  
Author(s):  
CHRISTOPH METZGER-BODDIEN ◽  
ANJA BOSTEL ◽  
JOHANNES KEHLE
Keyword(s):  
Pcr Amplification ◽  
Reference Method ◽  
Evaluation Study ◽  
Microtiter Plate ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacteriological Method ◽  
Contaminated Food ◽  
Pure Cultures ◽  
Sensitivity Specificity

A commercially available PCR kit (AnDiaTec Salmonella sp. PCR-ELISA) was developed and evaluated for the detection of Salmonella sp. in food samples. The test is based on PCR amplification and hybridization of the amplified DNA to a microtiter plate followed by the detection of PCR product in the manner of an enzyme-linked immunosorbent assay test. The sensitivity and specificity were evaluated first with Salmonella pure cultures and artificially contaminated food samples, including food types for which an inhibition of the PCR reaction was expected. Both experiments proved a very good sensitivity, specificity, and reliability of the test with a very broad range of food types. In a second evaluation study, more than 1,100 food samples of different types were tested in parallel with the PCR method and with the International Standardization Organization 6579 bacteriological reference method. The results of this evaluation study and the results from other experiments on dilutions of artificially contaminated food samples led to the establishment of a positive-negative cutoff value (optical density at 450 nm of more than 0.9) with respect to the conventional bacteriological method. Using this positive-negative cutoff, 98% agreement to the bacteriological method was obtained.

scholarly journals Microbial food safety in Ghana: a meta-analysis

2012 ◽  
Vol 6 (12) ◽  
pp. 828-835 ◽  
Author(s):  
Courage Kosi Setsoafia Saba ◽  
Bruno Gonzalez-Zorn
Keyword(s):  
Food Safety ◽  
Meta Analysis ◽  
Food Contamination ◽  
Food Samples ◽  
Future Research ◽  
Fermented Foods ◽  
Food Research ◽  
Food Borne ◽  
Contaminated Food

Introduction: Food safety is a crucial factor in the growth of developing countries worldwide. In this study, we present a meta-analysis of microbiological food safety publications from Ghana. Methodology: The search words “Ghana food safety”, “Ghana food research”, and “Ghana food bacteria” were used to search for microbiological food safety publications with related abstracts or titles in PubMed, published between 1997 and 2009. We obtained 183 research articles, from which we excluded articles concerning ready-to-eat microbial fermented foods and waterborne microorganisms as well as articles without abstracts. The criteria used for analysis of these publications were based on an assessment of methodological soundness previously developed for use in the medical field, with some modifications incorporated. Results: The most predominant bacteria in Ghanain foods are Enterobacter spp., Citrobacter spp., Klebsiella spp. and Escherichia spp., which were found to be present in 65%, 50%, 46% and 38% respectively, of the food samples considered in the studies analysed. The most contaminated food samples were macaroni, salad, and milk. Although the methodological quality of the articles was generally sound, most of them did not give directions for future research. Several did not state possible reasons for differences between studies. Conclusion: The microbiological food contamination in Ghana is alarming. However, we found that the downward trend in publications of microbial food safety articles is appalling. Hence a concerted effort in research on food safety is needed in Ghana to help curb the incidence of preventable food-borne disease.

scholarly journals Epidemiological Considerations Concerning Food Poisoning Infections

2018 ◽  
Vol 2 (3) ◽  
Keyword(s):  
Salmonella Enteritidis ◽  
Food Poisoning ◽  
Disease Outbreak ◽  
Summer Season ◽  
Enteric Disease ◽  
Salmonella Spp ◽  
Control Activity ◽  
Food Borne ◽  
Contaminated Food ◽  
And Control

What we eat and drink can become contaminated by bacteria, viruses, parasites, toxins or chemicals that can cause food-borne disease or named usual food poisoning, which is for CDC a common but preventable problem today. That for anyone can get sick from eating contaminated food. During the last 2 years: 2016-2017 it was received data for a complex epidemiological and clinical study, with several interesting interpretations and conclusions in Transylvania region from Romania. There were 109 Food borne diseases, with 2049 sick cases, almost during the summer season. The out breaks appeared in Urban or Rural parts as well in family or collectivity situations. Most of the illness appeared in usual forms-70% or 22 as middle forms and only 5% have needed hospitalization and only 3% from were just emergency cases. In our cases the microbiological etiology was: Salmonella spp-67%, mostly Salmonella enteritidis in 29%. Also 14% were determined by Staphylococcus aureus. To prevent such diseases in population, there are some food safety recommended steps. For a useful prevent and control activity in Food born infection, it has to be put together specialists for: laboratory, epidemiology, and environmental health, as all other with possible enteric disease outbreak and therapy responsibilities.

scholarly journals Botulism in Brazil, 2000-2008: epidemiology, clinical findings and laboratorial diagnosis

2010 ◽  
Vol 52 (4) ◽  
pp. 183-186 ◽  
Author(s):  
Ruth Estela Gravato Rowlands ◽  
Christiane Asturiano Ristori ◽  
Giselle Ibette S. Lopez Lopes ◽  
Ana Maria Ramalho de Paula ◽  
Harumi Sakuma ◽  
...  
Keyword(s):  
Clostridium Botulinum ◽  
Type A ◽  
Food Samples ◽  
Clinical Findings ◽  
Control Measures ◽  
Clinical Samples ◽  
Mouse Bioassay ◽  
Detection And Identification ◽  
Food Borne ◽  
Type Ab

Botulism is a rare and potentially lethal illness caused by Clostridium botulinum neurotoxin. We describe the findings of a laboratorial investigation of 117 suspected cases of botulism reported to the surveillance system in Brazil from January 2000 to October 2008. Data on the number and type of samples analyzed, type of toxins identified, reporting of the number of botulism cases and transmission sources are discussed. A total of 193 clinical samples and 81 food samples were analyzed for detection and identification of the botulism neurotoxin. Among the clinical samples, 22 (11.4%) presented the toxin (nine type A, five type AB and eight with an unidentified type); in food samples, eight (9.9%) were positive for the toxin (five type A, one type AB and two with an unidentified type). Of the 38 cases of suspected botulism in Brazil, 27 were confirmed by a mouse bioassay. Laboratorial botulism diagnosis is an important procedure to elucidate cases, especially food-borne botulism, to confirm clinical diagnosis and to identify toxins in food, helping sanitary control measures.

scholarly journals Evaluation of a Dry, Rehydratable Film Method for Rapid Enumeration of Staphylococcus aureus

2000 ◽  
Vol 83 (5) ◽  
pp. 1096-1107 ◽  
Author(s):  
Patrick A Mach ◽  
Kathryn G Lindberg ◽  
Marlys E Lund
Keyword(s):  
Staphylococcus Aureus ◽  
Classical Method ◽  
Food Samples ◽  
Bacterial Strains ◽  
Plate Method ◽  
Rapid Enumeration ◽  
Contaminated Food ◽  
Pure Cultures ◽  
Time Required ◽  
Film Method

Abstract Results with the new 3M™ Petrifilm™ Rapid S. aureus Count (RSA) Plate method were compared with those of the classical Baird-Parker agar (BPA) method for detection and enumeration of Staphylococcus aureus. Studies on 219 bacterial strains demonstrated that the Petrifilm RSA plate is more sensitive than and as specific as the classical BPA method for confirmed identification of S. aureus. Counts of colonies from 71 pure cultures, 61 naturally contaminated food samples, and more than 750 artificially inoculated food samples showed that the Petrifilm RSA method was as effective as the classical BPA method for identification and enumeration of S. aureus. The Petrifilm RSA method gave results in one-third the time required for the classical method.

Enzyme Immunoassay - Membrane Filter Method for Detection of Salmonellae in Foods

1985 ◽  
Vol 48 (9) ◽  
pp. 790-793 ◽  
Author(s):  
JEFFREY M. FARBER ◽  
PEARL I. PETERKIN ◽  
ANTHONY N. SHARPE ◽  
JEAN-YVES D'AOUST
Keyword(s):  
Membrane Filter ◽  
Protein A ◽  
Food Samples ◽  
Filter Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salmonella Spp ◽  
Contaminated Food ◽  
Elisa Technique ◽  
Membrane Filter Method ◽  
Exact Correlation

An enzyme-linked immunosorbent assay (ELISA) technique using a horseradish peroxidase-protein A-Spicer Edwards antiserum complex was developed for the detection of Salmonella colonies on membrane filters. In pure culture, 64 Salmonella species tested gave a positive reaction (purple stain). Of 22 naturally contaminated food samples, there was an exact correlation between the AOAC hydrophobic grid-membrane filter procedure and the ELISA technique (40.9% positives). This technique is simple, requires little equipment and can be completed in less than 2.5 h, thus allowing the detection of Salmonella spp. in foods within 48 h from initiation of sampling.

Inhibition of Bacterial Biofilm Formation by Phytotherapeutics with Focus on Overcoming Antimicrobial Resistance

2020 ◽  
Vol 26 (24) ◽  
pp. 2807-2816 ◽  
Author(s):  
Yun Su Jang ◽  
Tímea Mosolygó
Keyword(s):  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Bacterial Biofilm ◽  
Experimental Investigations ◽  
Resistant Bacteria ◽  
Salmonella Spp ◽  
Food Borne ◽  
High Prevalence ◽  
Scientific Attention

: Bacteria within biofilms are more resistant to antibiotics and chemical agents than planktonic bacteria in suspension. Treatment of biofilm-associated infections inevitably involves high dosages and prolonged courses of antimicrobial agents; therefore, there is a potential risk of the development of antimicrobial resistance (AMR). Due to the high prevalence of AMR and its association with biofilm formation, investigation of more effective anti-biofilm agents is required. : From ancient times, herbs and spices have been used to preserve foods, and their antimicrobial, anti-biofilm and anti-quorum sensing properties are well known. Moreover, phytochemicals exert their anti-biofilm properties at sub-inhibitory concentrations without providing the opportunity for the emergence of resistant bacteria or harming the host microbiota. : With increasing scientific attention to natural phytotherapeutic agents, numerous experimental investigations have been conducted in recent years. The present paper aims to review the articles published in the last decade in order to summarize a) our current understanding of AMR in correlation with biofilm formation and b) the evidence of phytotherapeutic agents against bacterial biofilms and their mechanisms of action. The main focus has been put on herbal anti-biofilm compounds tested to date in association with Staphylococcus aureus, Pseudomonas aeruginosa and food-borne pathogens (Salmonella spp., Campylobacter spp., Listeria monocytogenes and Escherichia coli).

Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 160-166
Author(s):  
Seyedeh Hanieh Eshaghi Zadeh ◽  
Hossein Fahimi ◽  
Fatemeh Fardsanei ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Food Borne ◽  
Class 1 ◽  
Resistance Patterns ◽  
C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

Recovery tests of cytopathogenic viruses from artificially contaminated food samples

2009 ◽  
Vol 1 (3) ◽  
pp. 191-194
Author(s):  
R. Zoni ◽  
R. Zanelli ◽  
S. Salsi ◽  
M. E. Colucci ◽  
G. Sansebastiano
Keyword(s):  
Food Samples ◽  
Contaminated Food
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