Microbiological Quality of Beef Subprimals as Affected by Lactic Acid Sprays Applied at Various Points during Vacuum Storage

1997 ◽  
Vol 60 (7) ◽  
pp. 795-798 ◽  
Author(s):  
R. K. PRASAI ◽  
C. L. KASTNER ◽  
P. B. KENNEY ◽  
D. H. KROPF ◽  
D. Y. C. FUNG ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Storage Temperature ◽  
Microbiological Quality ◽  
Storage Period ◽  
Microbial Counts ◽  
Before And After ◽  
Main Effects ◽  
Vacuum Storage ◽  
Spray Treatment

Beef strip loins were sprayed with 1.5% (vol/vol) lactic acid before and/or after 14, 28, 56, 84, and 126 days of vacuum-packaged storage at −1.1 or 2°C to assess the effects on bacteria. Five different treatment combinations including a control (no spray treatment) and one treatment with prestorage acid spray followed by poststorage water spray (A/W) were evaluated. Compared to the controls, 97% of all acid-treated loins had lower microbial counts throughout the storage period. Prestorage acid spray (A/0) was more effective than poststorage acid spray (0/A)with the greatest effects (P < 0.05) being observed on day 28, where the bacterial population was reduced by 1.9 and 1.5 log CFU/cm2 for loins stored at −1.1 and 2°C, respectively. Microbial counts for loins with A/A(acid spray both before and after vacuum storage) or A/W treatments were lower than but not significantly different from A/0 (P > 0.05). Poststorage washing resulted in a minimal decontaminating effect, irrespective of the washing agent used. In addition, single spray treatment immediately after fabrication seems more effective and practical than a double spray. Temperature main effects showed that colder temperature (−1.1 versus 2°C) reduced (P < 0.03) microbial growth throughout the storage period. Salmonella contamination was not detected in any samples; however, 28% of the control and 4% of the spray-treated loins were positive for Listeria spp. Appropriate time of acid application in combination with colder storage temperature improved the microbiological quality of meat for at least four weeks.

scholarly journals Effects of several sanitisers for improving quality attributes of minimally processed Fragaria vesca strawberry

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 49-54 ◽  
Author(s):  
S. Nogales-Delgado ◽  
A.M. Fernández-León ◽  
J. Delgado-Adámez ◽  
M.T. Hernández-Méndez ◽  
D. Bohoyo-Gil
Keyword(s):  
Lactic Acid ◽  
Sodium Hypochlorite ◽  
Storage Period ◽  
Good Alternative ◽  
Sensory Attributes ◽  
Minimally Processed ◽  
Microbial Counts ◽  
Fresh Cut ◽  
Washing Water

In order to decrease microbial contamination, fresh-cut industry commonly uses sodium hypochlorite as a disinfection agent, however, the by-products such as trihalometanes and chloramines are potentially harmful for human health making necessary the search for alternative disinfectant agents. A comparative study on the effectiveness of different disinfection methods on the quality of minimally processed F. vesca strawberry is presented. The fruit was processed in a clean room through the following steps: reception, cutting, washing, draining, and packaging. The processed strawberries were packaged in thermally sealed polypropylene trays using passive modified atmosphere. During a storage period of 8 days at 4ºC, the quality parameters, sensory attributes, and microbial counts were determined. As conclusion, the use of lactic acid at a concentration of 2.5 g/l in the washing water was effective in reducing microbial counts, maintaining the sensory attributes and quality of the product during the storage. The present study demonstrates that the use of lactic acid in the washing water could be a good alternative of the use of sodium hypochlorite and suggests that strawberries could make an acceptable fresh-cut product.  

scholarly journals Microbiological quality of surimi-based imitation crab

2018 ◽  
Vol 51 (2) ◽  
pp. 134
Author(s):  
N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽  
A. ABRAHIM (Α. ΑΜΠΡΑΧΙΜ) ◽  
E. THEOLOGIDOU (Ε. ΘΕΟΛΟΓΙΔΟΥ) ◽  
Pr. KARAIOANNOGLOU (ΠΡ. ΚΑΡΑΪΩΑΝΝΟΓΛΟΥ) ◽  
A. KANSOUZIDOU (Α. ΚΑΝΣΟΥΖΙΔΟΥ)
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Storage Period ◽  
Coliform Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Microbial Indices

A survey was conducted to evaluate the microbiological quality of sliced, vacuumpacked, surimi-based imitation crab at the retail level, during a 3-month storage period. The roll shaped product imported from France, was sliced and packed in a fishery processing plant in the region of Thessaloniki. Twenty five samples were examined each month, and a total of 75 samples were examined during the three month storage period of the product. Psychrotropic, lactic acid and coliform bacteria, Escherichia coli I, yeasts, Staphylococcus aureus, Salmonella spp., Listeria spp. and Escherichia coli Θ157:Η7, were used as microbial indices, to evaluate the microbiological quality of the product. The following conclusions were derived from the evaluation of the results of the microbiological examinations. 1) The microbiological condition of the crab meat analogs examined on the 15th day of storage, was found satisfactory, since microbial populations were very low, while pathogenic bacteria were not detected in any of the examined samples. 2) The overall microbiological quality of the samples examined at the end of the second month of storage, was also good, since psychrotrophic and lactic acid bacteria exceeded 1Θ7 log cfu/g only in one sample), while coliform bacteria were not detected in 60% of the samples and Escherichia coli I was detected only in one sample. The mean value of the yeasts was 2.84 log cfu/g, while molds were not detected in any of samples examined. 3) The microbiological quality of the samples examined at the end of the three months storage period was not satisfactory, since the microbial indices in the majority of the samples (80%), were rather high. S. aureus, Salmonella spp, Listeria s pp. and E. coli 0157:H7 were not detected in any sample, during the three month storage period.

scholarly journals Microbiological quality of beef stored under different modified atmospheres

2020 ◽  
Vol 76 (12) ◽  
pp. 6471-2020
Author(s):  
KATARZYNA ŚMIECIŃSKA
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Microbial Growth ◽  
Microbiological Quality ◽  
The Other ◽  
Microbial Counts ◽  
Psychrotrophic Bacteria ◽  
Modified Atmospheres ◽  
Microbial Groups

The aim of this study was to analyze changes in the microbiological quality of Longissimus lumborum (LL) muscles collected from young Polish Holstein-Friesian Black-and-White (PHF BW) bulls and stored for 7, 14, and 21 days under different modified atmospheres (MA) (vacuum, 80% O2 + 20% CO2, 60% O2 + 30% CO2 + 10% N2, 40% CO2 + 60% N2, 30% CO2 + 70% Ar). Before and after the specified storage periods, crosssectional samples of the LL muscle were collected under aseptic conditions to determine total microbial counts, and the total counts of psychrotrophic bacteria and mesophilic lactic acid bacteria. The effects of storage time and MA composition on changes in the parameters analyzed were evaluated, and the effectiveness of argon in delaying microbial growth in cold-stored beef was determined. Between days 7 and 14 of storage, total microbial counts were lowest in meat packaged under the MA composed of 30% CO2 + 70% Ar, whereas between days 14 and 21 they were lowest in both MA packages containing O2. The greatest increase in total microbial counts between days 7 and 14 of storage was noted in the MAs containing O2, and between days 14 and 21 of storage in the MA containing Ar. Beef stored for 7 days in the MA containing 60% O2 was characterized by the lowest total microbial counts, whereas the highest total microbial counts were noted in samples stored for 14 days in the MA containing 80% O2. Between days 7 and 14 of storage, the counts of psychrotrophic bacteria increased significantly in all MAs. Between days 14 and 21 of storage, the counts of psychrotrophic bacteria did not increase in meat samples packaged under vacuum or the MA composed of 80% O2 + 20% CO2. The counts of psychrotrophs were significantly lower in beef stored for 7 days in the oxygen-free MAs than they were in both MA packages containing O2. The counts of psychrotrophic bacteria were significantly lower in beef stored for 14 days in the MA containing Ar, compared with samples packaged under vacuum and the MA containing 80% O2. Gas composition in MA packaging had no significant effect on total microbial counts or the counts of psychrotrophic bacteria in meat stored for 21 days. Between days 14 and 21 of storage, the counts of lactic acid bacteria did not increase significantly only in meat packaged in the MAs composed of 60% O2 + 30% CO2 + 10% N2 and 40% CO2 + 60% N2. After 7 days of storage, the counts of lactic acid bacteria were higher in beef packaged in oxygen-free MAs than they were in both MA packages containing O2. After 14 days of storage, the counts of lactic acid bacteria were higher in meat packaged in the MA composed of 80% O2 + 20% CO2 than they were in the other MAs. Beef samples stored for 21 days under the MA composed of 80% O2 + 20% CO2 were characterized by significantly lower counts of lactic acid bacteria, compared with samples stored under the other MAs, except for the MA composed of 40% CO2 + 60% N2. It can be concluded that beef can be stored in the MA composed of 30% CO2 + 70% Ar for shorter periods of time (up to 14 days). The modified atmosphere composed of 30% CO2 + 70% Ar contributed to slower microbial growth in meat and resulted in lower counts of psychrotrophs and lactic acid bacteria, relative to the other samples. However, there is a need for further research on different concentrations of argon in MA packages to confirm that this gas can effectively delay microbial growth in beef. Meat stored for 14 days in the MA composed of 80% O2 + 20% CO2 was characterized by the lowest microbiological quality. The composition of MAs had a minor influence on the growth rates of the microbial groups analyzed in beef stored for 21 days; certain changes were observed only in the counts of lactic acid bacteria.

scholarly journals The possibility of using lactic additives and sonochemical technology in the production of preserves

2020 ◽  
Vol 164 ◽  
pp. 06012
Author(s):  
Vera Demchenko ◽  
Irina Asfondiarova ◽  
Nina Katkova ◽  
Marina Ivanova ◽  
Elena Belokurova
Keyword(s):  
Ultrasonic Treatment ◽  
Storage Temperature ◽  
Food Additives ◽  
Microbiological Quality ◽  
Point Scale ◽  
Resource Saving ◽  
High Quality ◽  
Processing Power ◽  
Fish Product

The priority in improving the existing methods of processing fish is salting with the use of food additives with different functional orientations. The aim of the study was the development of preserves with the introduction of lactate-containing additives and using sonochemical technologies; examination of the quality of the products obtained according to organoleptic and microbiological safety indicators. The article proposes a resource-saving technology for the production of fish products with prolonged shelf life due to the use of a complex food additive consisting of lactic, acetic, propionic acids and their salts in different proportions with subsequent ultrasonic treatment using the ultrasonic generator of the Wave series model UZTA-0 2/22-OM. There was established the optimal mode of using the ultrasonic processing power of 75 W / m2 in the manufacture of fish preserves from herring. Organoleptic and microbiological quality indicators were studied during storage of preserved products with the addition of additives and ultrasonic treatment compared to the control. Organoleptic assessment was carried out on a five-point scale, microbiological indicators were studied by standard methods. The Dilactin Forte Plus dietary supplement in the composition of preserves in the amount of 3% and in combination with the use of sonochemical technologies makes it possible to obtain a safe high-quality fish product. Manufactured preserves at a storage temperature of 0 ± 2 ° C can maintain their high quality for 5 months.

Microbiological Quality of Commercial Tofu

1984 ◽  
Vol 47 (3) ◽  
pp. 177-181 ◽  
Author(s):  
T. G. REHBERGER ◽  
L. A. WILSON ◽  
B. A. GLATZ
Keyword(s):  
Microbiological Quality ◽  
Plate Count ◽  
Microbial Counts ◽  
Colony Forming Units ◽  
Low Levels ◽  
Microbial Groups ◽  
Yeasts And Molds ◽  
Processing Techniques

A study was done to investigate the microbiological quality of commercial tofu available in local retail outlets. A sampling method was first developed to obtain accurate and representative microbial counts of individual pieces of tofu. Plate count determination of total aerobic organisms, psychrotrophs, coliforms, sporeformers, yeasts and molds, and staphylococci were made on 60 tofu samples (representing three lots each of four different brands) obtained within 24 h after delivery to the retail store. In addition, for two brands that provided manufacturer's pull dates, the same microbial counts were obtained for samples stored in the laboratory at 10°C until the pull date. Of the tofu sampled immediately after purchase, 83% of the lots tested had total counts greater than 106 colony-forming units (CFU)/g and psychrotrophic counts greater than 104 CFU/g. In addition, 67% of the lots tested had confirmed coliform counts greater than 103 CFU/g. Very low levels (less than 10 CFU/g) of all other microbial groups tested for were found in the majority of lots. Samples held until the manufacturer's pull date contained higher total and psychrotrophic counts but lower or stable counts of other organisms compared with samples tested immediately after purchase. To improve the microbiological quality of tofu, processors need to reduce initial loads by improving sanitation and processing techniques, and retailers should provide more consistent and colder refrigerated storage.

scholarly journals Tokat Bez Sucuğunun Mikrobiyolojik Kalitesinin İncelenmesi

2020 ◽  
Vol 8 (12) ◽  
pp. 2683-2694
Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Microbiological Parameters

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform

Use of the Systems Approach To Determine the Fate of Escherichia coli O157:H7 on Fresh Lettuce and Spinach

2009 ◽  
Vol 72 (7) ◽  
pp. 1560-1568 ◽  
Author(s):  
HELGA J. DOERING ◽  
MARK A. HARRISON ◽  
RUTH A. MORROW ◽  
WILLIAM C. HURST ◽  
WILLIAM L. KERR
Keyword(s):  
Escherichia Coli ◽  
Systems Approach ◽  
Storage Temperature ◽  
Storage Period ◽  
Categorical Variables ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Field Temperature ◽  
Before And After ◽  
Handling Practices

Lettuce and spinach inoculated with Escherichia coli O157:H7 were processed and handled in ways that might occur in commercial situations, including variations in holding times before and after product cooling, transportation conditions and temperatures, wash treatments, and product storage temperatures and times. Populations of background microflora and E. coli O157:H7 were enumerated after each step in the system. Data analysis was done to predict response variables with a combination of independent categorical variables. Field temperature, time before cooling, and wash treatment significantly affected E. coli O157:H7 populations on both products. The lowest populations of E. coli O157:H7 were encountered when precool time was minimal, lettuce was washed with chlorine, and storage temperature was 4°C. For lettuce, field and transportation temperature were not important once the storage period started, whereas after 2 days E. coli O157:H7 populations on packaged baby spinach were not affected by field temperature. On chopped iceberg lettuce and whole leaf spinach that was packaged and stored at 4°C, E. coli O157:H7 contamination could still be detected after typical handling practices, although populations decreased from initial levels in many cases by at least 1.5 log units. In abusive cases, where populations increased, the product quality quickly deteriorated. Although E. coli O157:H7 levels decreased on products handled and stored under recommended conditions, survivors persisted. This study highlights practices that may or may not affect the populations of E. coli O157:H7 on the final product.

scholarly journals Moderate heat treatments enhance the quality of traded natural shea butter

2020 ◽  
Vol 22 (1) ◽  
pp. 17-26
Author(s):  
A.O. Oyedele ◽  
O.A. Igbeneghu ◽  
T.I. Alade ◽  
O.O. Akinmusire
Keyword(s):  
Microbiological Quality ◽  
Poor Quality ◽  
Heat Treatments ◽  
Shea Butter ◽  
Physicochemical Quality ◽  
Melting Temperatures ◽  
Before And After ◽  
Human Use ◽  
C 30

Natural shea butter (NSB), extracted by traditional methods resulting in its poor quality, is nevertheless widely traded within Africa and beyond due to its several useful applications. This study examined effects of simulated laboratory/domestic heat treatments on quality of the commodity obtained from a cross section of Nigerian markets. Physicochemical and microbiological qualities of NSB samples procured from four selected  markets located across three Nigerian states were evaluated by standard methods before and after graduated thermal stress treatments from 50 through 120 °C over 5, 15, or 30 min durations, respectively, and filtration at 60 °C. Mean physicochemical quality values of NSB samples determined, namely: specific gravity at 29 °C (0.90-0.94); softening, melting temperatures (33-36, 36-39 °C; respectively); acid, iodine, and saponification values (10.5- 29.3, 46.4-59.1, 110-470; respectively), were not adversely or significantly altered by the thermal treatment types and stresses. Whereas all untreated NSB samples demonstrated microbial contamination (total viable counts: 3 6 10 -10 cfu/g) with Pseudomonas, Klebsiella, Staphylococcus, Bacillus, Aspergillus, or Candida species, the graduated heat treatments produced varied sanitizing effects. Higher temperatures (100, 120 °C) gave greater and more rapid cleansing action than the lower temperatures (50, 75 °C), both intensity-ranges being aided by length of holding time. Hot filtration eliminated all the NSB contaminants. In conclusion, while untreated NSB is found grossly contaminated by microbes, unhygienic and unsafe for human use, this study has demonstrated efficient contaminants-cleansing action of heat treatments (³100 °C × ³ 30 min) on NSB, and the total sanitizing effect of hot filtration process. Key words: Natural shea butter, Physicochemical quality, Microbiological quality, Heat treatment, Hot filtration.

scholarly journals Physico-Chemical Characteristics and Microbiological Quality of Broiler Chicken Pectoralis Major Muscle Subjected to Different Storage Temperature and Duration

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 741
Author(s):  
Muhamad Faris Ab Aziz ◽  
Muhammad Nizam Hayat ◽  
Ubedullah Kaka ◽  
Nitty Hirawaty Kamarulzaman ◽  
Awis Qurni Sazili
Keyword(s):  
Meat Quality ◽  
Broiler Chicken ◽  
Storage Temperature ◽  
Pectoralis Major Muscle ◽  
Microbiological Quality ◽  
Pectoralis Major ◽  
Cooking Loss ◽  
Storage Duration ◽  
Physico Chemical

Storage temperature and duration plays an important role in meat processing. Observations in poultry processing plants have shown a serious deviation in storage condition compared to the recommended procedures. Furthermore, there is still a paucity of evidence on the effects of storage temperature and duration on meat quality and microbial population. The aim of this study was to determine the effects of different temperature and duration during storage on physico-chemical properties and microbiological quality of broiler chicken Pectoralis major muscle. Eighty birds were slaughtered and processed, following which the packed boneless breast (PBB) (each bird was to provide two breast muscle samples; left breast and right breast) was divided into four groups, each consisted of 40 PBB. Each group was subsequently assigned to storage either at 4 °C, −10 °C, −18 °C or −40 °C, for 24 h before 20 PBB samples from each group were transported to the respective laboratory for meat quality and microbiological analysis. The remaining 20 PBB from each storage temperature were stored for 72 h before being transported for analysis. Results have shown significant increases in drip loss and cooking loss as the storage temperature decreases. Similarly, storage duration significantly affected cooking loss, of which, samples stored for 72 h exhibited higher cooking loss compared to those stored for 24 h. For color, significant differences were only observed in lightness (L*) and redness (a*) values. Longer duration of storage had significantly improved tenderness whereby, samples that have been stored for a shorter duration presented higher pH values. Populations of coliform and Salmonella decreased significantly with decreasing temperature and increasing storage duration.

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