Prevalence of Salmonella enterica and Listeria monocytogenes Contamination in Foods of Animal Origin in Italy

2005 ◽  
Vol 68 (8) ◽  
pp. 1729-1733 ◽  
Author(s):  
L. BUSANI ◽  
A. CIGLIANO ◽  
E. TAIOLI ◽  
V. CALIGIURI ◽  
L. CHIAVACCI ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Salmonella Enterica ◽  
Dairy Products ◽  
Meat Products ◽  
Food Samples ◽  
Control Measures ◽  
Poultry Meat ◽  
Animal Origin ◽  
Grocery Products

The present survey collected and analyzed the results of routine testing for Salmonella enterica and Listeria monocytogenes on foods of animal origin submitted for official controls in Italy during 2001 to 2002. Salmonella was detected in 2.2% of 71,643 food samples examined, and the isolation rates ranged from 9.9% for raw poultry meat to less than 0.1% for dairy products. Isolation rates were also high in raw pork (4.9%) and processed meats (5.3%), which often involved pork. Low rates were observed in seafood (0.5%) and in ready-to-eat foods, such as grocery products (0.7%) and ice creams (0.1%). Serotyping showed that approximately 50% of the isolates belonged to the serotypes most commonly isolated from humans in Italy, thus confirming that most cases of human salmonellosis have a foodborne origin. Levels of L. monocytogenes were higher than what is accepted by the current regulation in 2.4% of 42,300 food samples. The positivity rates ranged from 10.3% in raw pork to none in eggs and egg products. Contamination rates were higher in other meat products (between 2 and 5%) and fish (6.5%) than in cheeses (1.1%) and other dairy products (0.6%). Routine control activities on the microbial contamination of foods can generate data with statistical and epidemiological value. Such data can be used as a basis for estimating the exposure of consumers to foodborne pathogens, following the trends of contamination over time, and evaluating the effects of control measures on the contamination of food.

scholarly journals Meta-analysis of Escherichia coli O157 prevalence in foods of animal origin in Turkey

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Serhat Al ◽  
Aytaç Akçay ◽  
Elif Çelik ◽  
Güven Güngör ◽  
Candan Güngör ◽  
...  
Keyword(s):  
Dairy Products ◽  
Meta Analysis ◽  
Food Samples ◽  
Red Meat ◽  
Poultry Meat ◽  
Animal Origin ◽  
Accurate Estimation ◽  
Prevalence Studies ◽  
E Coli ◽  
Milk And Dairy Products

AbstractThe present study aims to analyze the prevalence of E. coli O157 detected in foods of animal origin by meta-analysis. The prevalence of E. coli O157 detected in the different studies was combined to provide a common prevalence estimate, and heterogeneities between studies were investigated. The study material consisted of 49 studies investigating E. coli O157 prevalence in a total of 9600 food samples, including milk and dairy products, red meat and products, poultry meat and products, and cold appetizers between the years 1997-2019 in Turkey. In the meta-analysis, the Der-Simonian-Laird method was used. Meta-analyses were performed using the R 3.6.1. As a result of the meta-analysis, the common prevalence of E. coli O157 was 0.024 (0.018-0.029). As a result of the Egger’s Linear Regression Test, the study samples were found to be biased (t-value=6.092, P<0.001). To determine the source of heterogeneity between studies, sub-group and meta-regression analyses were performed in milk and dairy products, red meat and products, poultry meat and products, and ready-to-eat foods (RTEs). Accordingly, the prevalence of E. coli O157 in milk and dairy products, red meat and products, poultry meat and products, and RTEs was determined as 0.017, 0.031, 0.023, and 0.080 in Turkey, respectively. This study provides a stronger and more accurate estimation of the prevalence of E. coli O157 in foods of animal origin with the meta-analysis by eliminating inconsistencies in the effect of the sampling size of independent prevalence studies. However, in order to obtain accurate prevalence results in practice, it is necessary carefully to select the studies to be included in the analysis, to use the appropriate statistical model, and to interpret the results of the analysis correctly.

scholarly journals Dietary Intake, Nutritional Adequacy, and Food Sources of Protein and Relationships with Personal and Family Factors in Spanish Children Aged One to < 10 Years: Findings of the EsNuPI Study

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1062
Author(s):  
Casandra Madrigal ◽  
María José Soto-Méndez ◽  
Ángela Hernández-Ruiz ◽  
Teresa Valero ◽  
Federico Lara Villoslada ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Total Protein ◽  
Dairy Products ◽  
Protein Intake ◽  
Meat Products ◽  
Food Sources ◽  
Animal Origin ◽  
Total Protein Intake ◽  
Fish And Shellfish ◽  
Milk And Dairy Products

Diet in the first years of life is an important factor in growth and development. Dietary protein is a critical macronutrient that provides both essential and nonessential amino acids required for sustaining all body functions and procedures, providing the structural basis to maintain life and healthy development and growth in children. In this study, our aim was to describe the total protein intake, type and food sources of protein, the adequacy to the Population Reference Intake (PRI) for protein by the European Food Safety Authority (EFSA), and the Recommended Dietary Allowance (RDA) by the Institute of Medicine (IoM). Furthermore, we analyzed whether the consumption of dairy products (including regular milk, dairy products, or adapted milk formulas) is associated with nutrient adequacy and the contribution of protein to diet and whole dietary profile in the two cohorts of the EsNuPI (in English, Nutritional Study in the Spanish Pediatric Population) study; one cohort was representative of the Spanish population from one to < 10 years old (n = 707) (Spanish reference cohort, SRS) who reported consuming all kinds of milk and one was a cohort of the same age who reported consuming adapted milk over the last year (including follow-on formula, growing up milk, toddler’s milk, and enriched and fortified milks) (n = 741) (adapted milk consumers cohort, AMS). The children of both cohorts had a high contribution from protein to total energy intake (16.79% SRS and 15.63% AMS) and a high total protein intake (60.89 g/day SRS and 53.43 g/day AMS). We observed that protein intake in Spanish children aged one to < 10 years old was above the European and international recommendations, as well as the recommended percentages for energy intakes. The main protein sources were milk and dairy products (28% SRS and 29% AMS) and meat and meat products (27% SRS and 26% AMS), followed by cereals (16% SRS and 15% AMS), fish and shellfish (8% in both cohorts), eggs (5% SRS and 6% AMS), and legumes (4% in both cohorts). In our study population, protein intake was mainly from an animal origin (meat and meat products, milk and dairy products, fish and shellfish, and eggs) rather than from a plant origin (cereals and legumes). Future studies should investigate the long-term effect of dietary protein in early childhood on growth and body composition, and whether high protein intake affects health later in life.

scholarly journals Citrobacter braakii Yield False-Positive Identification as Salmonella, a Note of Caution

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2177
Author(s):  
Joanna Pławińska-Czarnak ◽  
Karolina Wódz ◽  
Magdalena Kizerwetter-Świda ◽  
Tomasz Nowak ◽  
Janusz Bogdan ◽  
...  
Keyword(s):  
False Positive ◽  
Salmonella Enterica ◽  
Meat Products ◽  
Animal Origin ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
False Positive Identification ◽  
Food Of Animal Origin ◽  
Meat Samples

Background: Globally, Salmonella enterica is one of the leading causes of foodborne illness in humans. Food of animal origin is obligatorily tested for the presence of this pathogen. Unfortunately, in meat and meat products, this is often hampered by the presence of background microbiota, which may present as false-positive Salmonella. Methods: For the identification of Salmonella spp. from meat samples of beef, pork, and poultry, the authorized detection method is PN-EN ISO 6579-1:2017-04 with the White–Kauffmann–Le Minor scheme, two biochemical tests: API 20E and VITEK II, and a real-time PCR-based technique. Results: Out of 42 presumptive strains of Salmonella, 83.3% Salmonella enterica spp. enterica, 14.3% Citrobacter braakii, and 12.4% Proteus mirabilis were detected from 180 meat samples. Conclusions: Presumptive strains of Salmonella should be identified based on genotypic properties such as DNA-based methods. The aim of this study was the isolation and identification of Salmonella spp. from miscellaneous meat sorts: beef, pork, and poultry.

Low Prevalence of Listeria monocytogenes in Foods from Italy

2007 ◽  
Vol 70 (6) ◽  
pp. 1507-1512 ◽  
Author(s):  
L. LATORRE ◽  
A. PARISI ◽  
R. FRACCALVIERI ◽  
G. NORMANNO ◽  
M. C. NARDELLA LA PORTA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogen ◽  
Poultry Meat ◽  
Animal Origin ◽  
Smoked Salmon ◽  
Hygienic Measures ◽  
Seafood Products ◽  
Common Serotypes ◽  
Meat Samples ◽  
Low Prevalence

Listeria monocytogenes is an important foodborne pathogen that causes gastrointestinal disorders, and, especially in immunocompromised people, serious extraintestinal diseases, such as septicemia and meningitis, as well as abortion in pregnant women. Many foods, from both plant and animal origin, have been involved in listeriosis outbreaks. This article reports the results of a 12-year survey (1993 through 2004) on the presence of L. monocytogenes in several kinds of food marketed in Italy. Of 5,788 analyzed samples, 121 (2.1%) were contaminated with L. monocytogenes. The highest prevalence was found in smoked salmon (10.6%) and in poultry meat samples (8.5%) and the lowest in red meat (0.3%). L. monocytogenes was not found in 154 samples of fresh seafood products. Fifty-two isolates were also serotyped by the agglutination method. The most common serotypes detected in the 52 strains tested were 1/2a (36.5%), followed by 1/2c (32.8%), 1/2b (13.5%), 4b (11.5%), 3a (3.8%), and 3b (1.9%). The results of the present study showed low levels of L. monocytogenes in the analyzed samples. A total of 61.5% of the 52 L. monocytogenes strains analyzed belonged to serotypes 1/2a, 1/2b, and 4b, namely the serovars that are most commonly involved in extraintestinal human listeriosis outbreaks. In the ready-to-eat samples, these three serotypes were 40.0% (1/2a), 17.1% (1/2b), and 14.3% (4b). This finding highlights the need to implement strict hygienic measures during the production, distribution, and sale of foods to reduce the risk of foodborne listeriosis in humans to an acceptable level.

Combination of Immunomagnetic Separation and Polymerase Chain Reaction for the Simultaneous Detection of Listeria monocytogenes and Salmonella spp. in Food Samples

2001 ◽  
Vol 64 (11) ◽  
pp. 1744-1750 ◽  
Author(s):  
HSIEN-YEE HSIH ◽  
HAU-YANG TSEN
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Simultaneous Detection ◽  
Meat Products ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Pcr Method ◽  
Polymerase Chain

A method that combined the immunomagnetic separation (IMS) technique and the multiplex polymerase chain reaction (PCR) method (i.e., the IMS-mPCR method) was developed for simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. When only the multiplex PCR method was used, it was found that if cell numbers of each of the two target organisms (L. monocytogenes and Salmonella spp.) were above the detection limit, but differed by more than 2 logs—e.g., n × 107 to n × 104 or n × 106 to n × 103—the organism presenting the lower numbers might go undetected. Following the enrichment step with universal preenrichment (UP) broth, if an IMS method using equal quantities of anti-Listeria and anti-Salmonella immunomagnetic beads was performed prior to PCR, both pathogens could be detected unambiguously. Such results could be obtained for target organisms in food samples, such as milk, dairy, and meat products, if similar enrichment and IMS steps were performed prior to PCR.

scholarly journals Population Genetic Structure of Listeria monocytogenes Strains as Determined by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

2016 ◽  
Vol 82 (18) ◽  
pp. 5720-5728 ◽  
Author(s):  
Clémentine Henri ◽  
Benjamin Félix ◽  
Laurent Guillier ◽  
Pimlapas Leekitcharoenphon ◽  
Damien Michelon ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Dairy Products ◽  
Population Genetic ◽  
Meat Products ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
Food Matrices

ABSTRACTListeria monocytogenesis a ubiquitous bacterium that may cause the foodborne illness listeriosis. Only a small amount of data about the population genetic structure of strains isolated from food is available. This study aimed to provide an accurate view of theL. monocytogenesfood strain population in France. From 1999 to 2014, 1,894L. monocytogenesstrains were isolated from food at the French National Reference Laboratory forL. monocytogenesand classified according to the five risk food matrices defined by the European Food Safety Authority (EFSA). A total of 396 strains were selected on the basis of different pulsed-field gel electrophoresis (PFGE) clusters, serotypes, and strain origins and typed by multilocus sequence typing (MLST), and the MLST results were supplemented with MLST data available from Institut Pasteur, representing human and additional food strains from France. The distribution of sequence types (STs) was compared between food and clinical strains on a panel of 675 strains. High congruence between PFGE and MLST was found. Out of 73 PFGE clusters, the two most prevalent corresponded to ST9 and ST121. Using original statistical analysis, we demonstrated that (i) there was not a clear association between ST9 and ST121 and the food matrices, (ii) serotype IIc, ST8, and ST4 were associated with meat products, and (iii) ST13 was associated with dairy products. Of the two major STs, ST121 was the ST that included the fewest clinical strains, which might indicate lower virulence. This observation may be directly relevant for refining risk analysis models for the better management of food safety.IMPORTANCEThis study showed a very useful backward compatibility between PFGE and MLST for surveillance. The results enabled better understanding of the population structure ofL. monocytogenesstrains isolated from food and management of the health risks associated withL. monocytogenesfood strains. Moreover, this work provided an accurate view ofL. monocytogenesstrain populations associated with specific food matrices. We clearly showed that some STs were associated with food matrices, such as meat, meat products, and dairy products. We opened the way to source attribution modeling in order to quantify the relative importance of the main food matrices.

scholarly journals INDICATION OF LISTERIA MONOCYTOGENES IN MEAT AND MEAT PRODUCTS IN THE TERRITORY OF AGRICULTURAL PROVINCE

2019 ◽  
Vol 96 (6) ◽  
pp. 590-593
Author(s):  
M. A. Sinelnikova ◽  
Luybov S. Buzoleva ◽  
N. Yu. Bespechuk ◽  
G. G. Koltun
Keyword(s):  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Meat Products ◽  
Poultry Meat ◽  
Occurrence Rate ◽  
Meat Production ◽  
Customs Union ◽  
Private And Public ◽  
Constant Quality

In recent decades, the majority of outbreaks of listeriosis with a high percentage of deaths was caused by the consumption of food products, including meat and meat products. One of the main principles of prevention of listeriosis in humans and animals is a constant quality control of food and feeds. The district of the Ussurisk city specializes mainly in the production of agricultural products. Meat production work on the raw materials of local origin, and the imported meat is also used. There was executed a study concerning the possibility of the contamination of meat products of Listeria monocytogenes on the territory of the agricultural province of the district of the Ussurisk city for the period from 2012 to 2015. A total of 21491 sample products were investigated according to rules “Common sanitary and epidemiological and hygienic requirements for goods subject to sanitary-and-epidemiologic supervision (control)” of the Customs Union (Chapter II, section 1) and the Technical Regulations of the Customs Union № 880 “About security of food “(Appendix 1). The presence of 45 positive cases of L. monocytogenes was revealed, at that the most of them have been found in meat products and poultry meat. Observations of the occurrence rate of L. monocytogenes in meat of the imported production for a number of years show these the pathogenic bacteria to be isolated every year in 40% - 66.6% of cases (of all positive cases of isolation from meat). This meat was imported mainly from countries such as Brazil, Paraguay, New Zealand, Austria, England, Uruguay. A large proportion of meat and meat products, contaminated by L. monocytogenes enters the territory of Primorsky Kray from Latin America. It is important to note that meat and meat products contaminated by these pathogens were also produced in the territory of the district of the city of Ussurisk. In connection with it there is obvious the need as for further continuation of monitoring products on the market, as a survey of agricultural animals of private and public farms in the district.

Occurrence and Characteristics of Listeria monocytogenes in Ready-to-Eat Meat Products in Poland

2020 ◽  
Vol 83 (6) ◽  
pp. 1002-1009 ◽  
Author(s):  
ELŻBIETA MAĆKIW ◽  
MONIKA STASIAK ◽  
JOANNA KOWALSKA ◽  
KATARZYNA KUCHAREK ◽  
DOROTA KORSAK ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Monitoring Program ◽  
Meat Products ◽  
Food Samples ◽  
Potential Hazard ◽  
Significant Group ◽  
Official Control ◽  
Safety Risks ◽  
Low Incidence

ABSTRACT Listeria monocytogenes is a potential hazard for food safety and therefore for public health. The aim of the present study was to determine the prevalence and characteristics of L. monocytogenes in Polish ready-to-eat (RTE) meat products for retail sale. Among the 184,439 food samples collected within the framework of a national official control and monitoring program, only 0.3% were positive for L. monocytogenes. A significant group of products that did not meet the criteria were RTE meat products. This group accounted for 40% of all noncompliant samples. Seventy L. monocytogenes isolates from these RTE meat products (meat, sausages, and delicatessen products with meat) were examined. The majority of the tested isolates (51%) belonged to serogroup 1/2a-3a followed by 1/2c-3c (21%), 1/2b-3b-7 (14%), and 4ab-4b-4d-4e (13%). Serogroup 4a-4c was not present among the tested isolates. All L. monocytogenes isolates harbored the virulence-associated genes inlA, inlC, inlJ, and lmo2672. The llsX marker was detected in 12 (17%) of the 70 isolates. Ampicillin resistance was the most common resistance phenotype and was identified in 83% of the L. monocytogenes isolates. A low incidence of resistance to amoxicillin–clavulanic acid (6% of isolates) was also detected. All L. monocytogenes isolates were susceptible to chloramphenicol, gentamicin, ciprofloxacin, meropenem, sulfamethoxazole-trimethoprim, tetracycline, and erythromycin. This work provides useful information regarding contamination of RTE meat products with L. monocytogenes, which may have implications for food safety risks. HIGHLIGHTS

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