Antimicrobial Resistance and Molecular Subtyping of Campylobacter jejuni and Campylobacter coli from Retail Meats

2011 ◽  
Vol 74 (4) ◽  
pp. 616-621 ◽  
Author(s):  
X. WANG ◽  
S. ZHAO ◽  
H. HARBOTTLE ◽  
T. TRAN ◽  
K. BLICKENSTAFF ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Relatedness ◽  
Positive Association ◽  
Restriction Enzymes ◽  
Campylobacter Coli ◽  
Erythromycin Resistance ◽  
Retail Meats ◽  
Retail Meat ◽  
Meat Samples ◽  
Sequence Types

Campylobacter isolates (n = 297; 202 C. jejuni and 95 C. coli isolates) recovered from 2,513 retail meat samples (chicken breasts, ground turkey, ground beef, and pork chops) were examined for antimicrobial susceptibility. The isolates were further analyzed for genetic relatedness by pulsed-field gel electrophoresis (PFGE) using SmaI and KpnI restriction enzymes, and a subset of isolates (n = 174) were subtyped by multilocus sequence typing (MLST). The resistance most frequently observed was that to doxycycline (27.6%), followed by ciprofloxacin (13.8%) and erythromycin (6.4%). All isolates were susceptible to gentamicin and meropenem. C. coli showed higher resistance to doxycycline than did C. jejuni (42.1 versus 20.8%) and lower resistance to ciprofloxacin than did C. jejuni (10.5 versus 15.3%). Erythromycin resistance was only observed in C. coli. PFGE using SmaI plus KpnI digestion generated 168 clusters from 297 isolates: 115 from C. jejuni and 53 from C. coli. MLST revealed 44 sequence types (STs) under 10 clonal complexes from 120 C. jejuni and 27 STs under two clonal complexes from 54 C. coli. There was a positive association between PFGE and STs; however, PFGE showed greater discriminatory power than MLST. Subtyping data did not correlate with antimicrobial resistance phenotypes.

scholarly journals Hyper-Aerotolerant Campylobacter coli from Duck Sources and Its Potential Threat to Public Health: Virulence, Antimicrobial Resistance, and Genetic Relatedness

2019 ◽  
Vol 7 (11) ◽  
pp. 579 ◽  
Author(s):  
Jae-Ho Guk ◽  
Junhyung Kim ◽  
Hyokeun Song ◽  
Jinshil Kim ◽  
Jae-Uk An ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Inhibitory Concentration ◽  
Genetic Relatedness ◽  
Control Strategies ◽  
Campylobacter Coli ◽  
Potential Threat ◽  
High Level ◽  
Sequence Types ◽  
Level Resistance

Campylobacter, a common foodborne human pathogen, is considered sensitive to oxygen. Recently, aerotolerant (AT) Campylobacter jejuni with the ability to survive under aerobic stress has been reported. Here, we investigated the prevalence of hyper-aerotolerant (HAT) Campylobacter coli from duck sources (118 carcasses and meat) and its characteristics to assess potential impacts on public health. Half of 56 C. coli isolates were HAT and most harbored various virulence genes including flaA, cadF, cdtA, ceuB, and wlaN. Moreover, 98.2% of C. coli isolates showed resistance to quinolones, including ciprofloxacin (CIP), and nine (16.1%) showed high-level resistance to ciprofloxacin (Minimum Inhibitory Concentration, MIC ≥ 32 μg/mL) and most of these were HAT. Based on genetic relatedness between C. coli from duck sources and those from human sources (PubMLST and NCBI), HAT isolates sharing the same MLST sequence types were significantly more prevalent than those not sharing the same sequence types as those from human sources. Therefore, HAT C. coli is prevalent in duck sources, and is most likely transmitted to humans through the food chain given its aerotolerance. This being so, it might pose a threat to public health given its virulence and antimicrobial resistance (AMR). This study will assist in improving control strategies to reduce farm-to-table HAT C. coli transmission to humans.

scholarly journals Antimicrobial Resistance of Campylobacter Isolates from Retail Meat in the United States between 2002 and 2007

2010 ◽  
Vol 76 (24) ◽  
pp. 7949-7956 ◽  
Author(s):  
S. Zhao ◽  
S. R. Young ◽  
E. Tong ◽  
J. W. Abbott ◽  
N. Womack ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Relatedness ◽  
Ground Beef ◽  
Sampling Period ◽  
The United States ◽  
Health Concern ◽  
Chicken Breast ◽  
Contamination Rate ◽  
Retail Meat ◽  
Meat Samples

ABSTRACT The emergence of antimicrobial resistance in Campylobacter spp. has been a growing public health concern globally. The objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Campylobacter spp. recovered by the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program. Retail meat samples (n = 24,566) from 10 U.S. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ground beef, and 6,148 pork chop samples, were analyzed. A total of 2,258 Campylobacter jejuni, 925 Campylobacter coli, and 7 Campylobacter lari isolates were identified. Chicken breast samples showed the highest contamination rate (49.9%), followed by ground turkey (1.6%), whereas both pork chops and ground beef had <0.5% contamination. The most common resistance was to doxycycline/tetracycline (46.6%), followed by nalidixic acid (18.5%), ciprofloxacin (17.4%), azithromycin and erythromycin (2.8%), telithromycin (2.4%), clindamycin (2.2%), and gentamicin (<0.1%). In a subset of isolates tested, no resistance to meropenem and florfenicol was seen. C. coli isolates showed higher resistance rates to antimicrobials, with the exception of doxycycline/tetracycline, than those seen for C. jejuni. Pulsed-field gel electrophoresis (PFGE) fingerprinting resulted in 1,226 PFGE profiles among the 2,318 isolates, with many clones being widely dispersed throughout the 6-year sampling period.

scholarly journals High Prevalence of Vancomycin Non-Susceptible and Multi-Drug Resistant Enterococci in Farmed Animals and Fresh Retail Meats in Bangladesh

2021 ◽  
Author(s):  
Mohammed A. Samad ◽  
Md Shahjalal Sagor ◽  
Muhammad Sazzad Hossain ◽  
Md Rezaul Karim ◽  
Mohammad Asheak Mahmud ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Health Concern ◽  
Specific Pcr ◽  
Retail Meats ◽  
High Prevalence ◽  
Retail Meat ◽  
Food Animals ◽  
Meat Samples ◽  
Line Drug ◽  
Isolated Species

Abstract The emergence of antimicrobial resistant Enterococcus spp., a leading cause of untreatable nosocomial infection, in food animals and dissemination to humans is a public health concern. The study was conducted to determine the prevalence and antimicrobial resistance, and virulence characteristics of Enterococcus faecalis and Enterococcus faecium in food animals and meats in Bangladesh. Enterococcus spp., were confirmed using sodA gene specific PCR, and antimicrobial resistance and virulence properties were characterized by PCR. Enterococcus spp. were recovered from 57% of the collected samples (n=201/352). Farm samples yielded significantly higher (p≤0.05) prevalence (62%) compared to retail meat samples (41%) and E. faecalis (52%) was most frequently isolated species. High proportions of isolates exhibited resistance to tetracycline (74%), erythromycin (65%) and ciprofloxacin (34%). Fifty-one isolates were vancomycin non-susceptible enterococci (VNSE), of which forty-seven were MDR and 20 were linezolid resistant, a last line drug for VNSE. Virulence factors such as gelatinase (gelE), aggregation factor (asa1) and sex pheromone (cpd) were detected along with vancomycin resistance gene (vanA, vanB and vanC2/C3) in VNSE isolates. The high prevalence of MDR enterococci in food animals and retail meats may lead to infection in consumers with concomitant reduced therapeutic options available for treatment.

scholarly journals Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 97
Author(s):  
Zunita Zakaria ◽  
Latiffah Hassan ◽  
Zawiyah Sharif ◽  
Norazah Ahmad ◽  
Rohaya Mohd Ali ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Multilocus Sequence Typing ◽  
Salmonella Enteritidis ◽  
Molecular Subtypes ◽  
Actin Binding ◽  
Diffusion Method ◽  
Genetic Characteristics ◽  
Meat Samples ◽  
Sequence Types

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.

scholarly journals Prevalence and Antimicrobial Resistance of Enterococcus Species Isolated from Retail Meats

2003 ◽  
Vol 69 (12) ◽  
pp. 7153-7160 ◽  
Author(s):  
Joshua R. Hayes ◽  
Linda L. English ◽  
Peggy J. Carter ◽  
Terry Proescholdt ◽  
Kyung Y. Lee ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Ground Beef ◽  
Chicken Breast ◽  
Grocery Stores ◽  
Predominant Species ◽  
Retail Meats ◽  
Resistance Patterns ◽  
Meat Samples ◽  
High Level

ABSTRACT From March 2001 to June 2002, a total of 981 samples of retail raw meats (chicken, turkey, pork, and beef) were randomly obtained from 263 grocery stores in Iowa and cultured for the presence of Enterococcus spp. A total of 1,357 enterococcal isolates were recovered from the samples, with contamination rates ranging from 97% of pork samples to 100% of ground beef samples. Enterococcus faecium was the predominant species recovered (61%), followed by E. faecalis (29%), and E. hirae (5.7%). E. faecium was the predominant species recovered from ground turkey (60%), ground beef (65%), and chicken breast (79%), while E. faecalis was the predominant species recovered from pork chops (54%). The incidence of resistance to many production and therapeutic antimicrobials differed among enterococci recovered from retail meat samples. Resistance to quinupristin-dalfopristin, a human analogue of the production drug virginiamycin, was observed in 54, 27, 9, and 18% of E. faecium isolates from turkey, chicken, pork, and beef samples, respectively. No resistance to linezolid or vancomycin was observed, but high-level gentamicin resistance was observed in 4% of enterococci, the majority of which were recovered from poultry retail meats. Results indicate that Enterococcus spp. commonly contaminate retail meats and that dissimilarities in antimicrobial resistance patterns among enterococci recovered from different meat types may reflect the use of approved antimicrobial agents in each food animal production class.

High Level of Antimicrobial Resistance in Campylobacter jejuni Isolated from Broiler Chickens in Estonia in 2005 and 2006

2007 ◽  
Vol 70 (8) ◽  
pp. 1940-1944 ◽  
Author(s):  
MATI ROASTO ◽  
KADRIN JUHKAM ◽  
TERJE TAMME ◽  
ARI HÖRMAN ◽  
LIIDIA HÄKKINEN ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Animal Origin ◽  
Campylobacter Coli ◽  
National Veterinary Institute ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
High Level

The development of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli is a matter of increasing concern. Because campylobacteriosis is transmitted to humans usually via food of animal origin, the presence of antimicrobial-resistant campylobacters in broiler chickens has important public health implications. The aim of our study was to analyze resistance patterns of C. jejuni isolated from fecal samples collected at a large Estonian chicken farm, from cecal contents collected at slaughterhouses, and from meat samples collected at the retail establishments in 2005 and 2006. A total of 131 C. jejuni isolates were collected over a 13-month period and tested by the broth microdilution VetMIC method (National Veterinary Institute, Uppsala, Sweden) to determine the MICs of various antimicrobials. Resistance to one or more antimicrobials was detected in 104 (79.4%) of the 131 isolates. High proportions of the isolates were resistant to enrofloxacin (73.3%) and nalidixic acid (75.6%). Multidrug resistance (resistance to three or more unrelated antimicrobials) was detected in 36 isolates (27.5%), all of which were resistant to enrofloxacin. Multidrug resistance was significantly associated with enrofloxacin resistance (P &lt; 0.01), and the use of enrofloxacin may select for multiresistant strains.

scholarly journals Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats

2008 ◽  
Vol 75 (1) ◽  
pp. 265-267 ◽  
Author(s):  
Shuaihua Pu ◽  
Feifei Han ◽  
Beilei Ge
Keyword(s):  
Staphylococcus Aureus ◽  
Grocery Stores ◽  
Methicillin Resistant ◽  
Isolation And Characterization ◽  
Retail Meats ◽  
Retail Meat ◽  
Mrsa Strains ◽  
Meat Samples ◽  
Pork Samples

ABSTRACT We investigated the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in 120 retail meat samples from 30 grocery stores in Baton Rouge, LA. S. aureus strains were recovered from 45.6% of pork samples and 20% of beef samples, whereas MRSA strains were isolated from six meat samples (five pork samples and one beef sample). The MRSA isolates were of two strain types (clones), one harboring Panton-Valentine leucocidin and belonging to pulsed-field gel electrophoresis type USA300 and the other one belonging to USA100.

scholarly journals Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1437
Author(s):  
Justice Opare Odoi ◽  
Sayo Takayanagi ◽  
Montira Yossapol ◽  
Michiyo Sugiyama ◽  
Tetsuo Asai
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Generation Cephalosporin ◽  
Resistant Bacteria ◽  
Third Generation ◽  
Phenotypic Resistance ◽  
Retail Meats ◽  
Cephalosporin Resistance ◽  
Retail Meat

Consumption of retail meat contaminated with antimicrobial-resistant (AMR) bacteria is a common route for transmitting clinically relevant resistant bacteria to humans. Here, we investigated the genotypic and phenotypic resistance profiles of intrinsic colistin-resistant (ICR) Enterobacterales isolated from retail meats. ICR Enterobacterales were isolated from 103 samples of chicken, 103 samples of pork, and 104 samples of beef purchased from retail shops in Japan, using colistin-containing media, and their antimicrobial susceptibility was examined. Serratia spp. (440 isolates) showed resistance to cefotaxime (19 isolates, 4.3%), tetracycline (15 isolates, 3.4%), and other antimicrobials (<1%). Hafnia spp. (136) showed resistance to cefotaxime (12 isolates, 8.6%), ceftazidime (four isolates, 2.9%), and tetracycline (two isolates, 1.4%). Proteus spp. (39) showed resistance to chloramphenicol (four isolates, 10.3%), sulfamethoxazole-trimethoprim (four isolates, 10.3%), cefotaxime (two isolates, 5.1%), kanamycin (two isolates, 5.1%), and gentamicin (one isolate, 2.6%). Cedecea spp. (22) were resistant to tetracycline (two isolates, 9.1%) whereas Morganella spp. (11) were resistant to tetracycline (four isolates, 36.4%) and chloramphenicol (one isolate, 9.2%). The resistance genes blafonA, blaACC, and blaDHA were detected in cefotaxime-resistant Serratia spp., Hafnia spp., and Morganella spp. isolates, respectively. This emergence of antimicrobial resistance in ICR Enterobacterales may pose a public health risk.

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