Occurrence and Phenotypic and Molecular Characterization of Antimicrobial Resistance of Salmonella Isolates from Food in Tunisia

2019 ◽  
Vol 82 (7) ◽  
pp. 1166-1175 ◽  
Author(s):  
AMAL BEN HASSENA ◽  
MARIAM SIALA ◽  
SONDA GUERMAZI ◽  
SONIA ZORMATI ◽  
RADHOUANE GDOURA ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Enteritidis ◽  
Foodborne Pathogen ◽  
Public Health Problem ◽  
Gene Families ◽  
Food Samples ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Salmonella Infections ◽  
Extended Spectrum

ABSTRACT Salmonella is a leading cause of foodborne diseases worldwide. The use of antibiotics in food-producing animals may contribute to the development of antimicrobial resistance in nontyphoidal Salmonella. The development of resistance to potent antimicrobials such as fluoroquinolones and extended-spectrum β-lactamases is a significant public health problem. The present study was conducted to examine the occurrence and antimicrobial resistance of Salmonella isolates obtained from food samples. Salmonella was cultured according to ISO 6579:2002 method, and antimicrobial resistance was evaluated with the Kirby-Bauer disk diffusion method. Forty-five Salmonella isolates were recovered, and a high Salmonella prevalence was detected in clams (7 of 20 samples, 35%), chicken (28 of 97 samples, 28.9%) and cow's milk (10 of 80 samples, 12.5%). Salmonella Enteritidis (n = 19) and Salmonella Kentucky (n = 18) were the most prevalent isolates. Multidrug resistance was found in 31.1% of the isolates (14 of 45); 84, 46, 28, and 17% of the isolates were resistant to nalidixic acid, ciprofloxacin, amoxicillin–clavulanic acid, and both ofloxacin and cefotaxime, respectively. The isolates resistant to cefotaxime were screened by PCR for the genes for TEM β-lactamase, extended-spectrum β-lactamases (CTX and OXA), and AmpC β-lactamases (FOX, MOX, DHA, ACC, CIT, and EBC). One Salmonella Kentucky isolate from milk harbored an AmpC gene (FOX), and the same serotype isolated from chicken carried the EBC AmpC determinant. The blaTEM gene was detected in all nonsusceptible isolates. We also screened isolates with reduced fluoroquinolone susceptibility for the presence of transferable plasmid-mediated quinolone resistance determinants. Three qnr genes (qnrB, qnrD, and qnrS) were detected in four isolates (two from milk and two from chicken). To our knowledge, this is the first report of the AmpC FOX and EBC gene families and the qnrD gene within a foodborne pathogen in Tunisia. These findings highlight the emergence of multidrug-resistant Salmonella isolates with decreased susceptibility to fluoroquinolones and third-generation cephalosporins, which are drugs commonly used for the treatment of Salmonella infections. HIGHLIGHTS

Prevalence and antimicrobial resistance of Listeria species and molecular characterization of Listeria monocytogenes isolated from retail ready-to-eat foods

2020 ◽  
Vol 367 (4) ◽  
Author(s):  
Seza Arslan ◽  
Fatma Özdemir
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Relatedness ◽  
Fragment Length Polymorphism ◽  
Food Samples ◽  
Diffusion Method ◽  
Consumer Health ◽  
Disk Diffusion Method ◽  
Listeria Species ◽  
Virulence Markers

ABSTRACT A wide variety of foods can be contaminated with Listeria species, especially L. monocytogenes. Ready-to-eat (RTE) foods are predominantly associated with human listeriosis caused by L. monocytogenes. Therefore, this study aimed to assess the presence of Listeria species in RTE foods and to characterize L. monocytogenes isolates by means of detection of virulence markers, serotypes and genetic relatedness. Of the 300 RTE food samples, 59 (19.7%) were positive for Listeria species: L. innocua (13.3%), L. monocytogenes (5%), L. welshimerii (2.3%), L. grayi subsp. murrayi (1.3%), L. grayi (1%), L. ivanovii (1%) and L. ivanovi subsp. londoniensis (0.3%). All L. monocytogenes isolates identified were positive for the actA, iap, inlA, inlB, inlC, inlJ, plcA and prfA virulence genes and biofilm. The isolates were serotyped as 1/2c (33.3%), 4b (26.7%), 1/2a (26.7%), 1/2b (6.7%) and 3c (6.7%) by the multiplex-PCR and agglutination methods. PCR-restriction fragment length polymorphism with AluI and MluCI resulted in three and two profiles, respectively. Pulsed-field gel electrophoresis differentiated the L. monocytogenes isolates into 15 ApaI and 12 AscI patterns. Antimicrobial resistance of all Listeria isolates was determined by the disk diffusion method. Most L. monocytogenes isolates were sensitive to antimicrobials used in the treatment of listeriosis. This study shows the presence of potential pathogenic and antimicrobial-resistant L. monocytogenes in RTE foods that may lead to consumer health risks.

scholarly journals Molecular Characterization of Cephalosporin and Fluoroquinolone Resistant Salmonella choleraesuis Isolated from Patients with Systemic Salmonellosis in Thailand

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 844
Author(s):  
Pichapak Sriyapai ◽  
Chaiwat Pulsrikarn ◽  
Kosum Chansiri ◽  
Arin Nyamniyom ◽  
Thayat Sriyapai
Keyword(s):  
Antimicrobial Resistance ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
Pulse Field ◽  
Diffusion Method ◽  
Third Generation ◽  
Disk Diffusion Method ◽  
Salmonella Choleraesuis ◽  
Antimicrobial Resistance Genes ◽  
Third Generation Cephalosporins

The antimicrobial resistance of nontyphoidal Salmonella has become a major clinical and public health problem. Southeast Asia has a high level of multidrug-resistant Salmonella and isolates resistant to both fluoroquinolone and third-generation cephalosporins. The incidence of co-resistance to both drug classes is a serious therapeutic problem in Thailand. The aim of this study was to determine the antimicrobial resistance patterns, antimicrobial resistance genes and genotypic relatedness of third-generation cephalosporins and/or fluoroquinolone-resistant Salmonella Choleraesuis isolated from patients with systemic salmonellosis in Thailand. Antimicrobial susceptibility testing was performed using the agar disk diffusion method, and ESBL production was detected by the combination disc method. A molecular evaluation of S. Choleraesuis isolates was performed using PCR and DNA sequencing. Then, a genotypic relatedness study of S. Choleraesuis was performed by pulse field gel electrophoresis. All 62 cefotaxime-resistant S. Choleraesuis isolates obtained from 61 clinical specimens were multidrug resistant. Forty-four isolates (44/62, 71.0%) were positive for ESBL phenotypes. Based on the PCR sequencing, 21, 1, 13, 23, 20 and 6 ESBL-producing isolates harboured the ESBL genes blaCTX-M-14, blaCTX-M-15, blaCTX-M-55, blaCMY-2, blaACC-1 and blaTEM-1, respectively. This study also found that nine (9/62, 14.5%) isolates exhibited co-resistance to ciprofloxacin and cefotaxime. All of the co-resistant isolates harboured at least one PMQR gene. The qnr genes and the aac(6′)-Ib-cr gene were the most prevalent genes detected. The QRDR mutation, including the gyrA (D87Y and D87G) and parC (T57S) genes, was also detected. PFGE patterns revealed a high degree of clonal diversity among the ESBL-producing isolates.

scholarly journals Serotypes and antimicrobial resistance profiles of Salmonella enterica recovered from clinical swine samples

2020 ◽  
Vol 13 (11) ◽  
pp. 2312-2318
Author(s):  
Siriporn Kongsoi ◽  
Suksun Chumsing ◽  
Darunee Satorn ◽  
Panisa Noourai
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
High Resistance ◽  
Salmonella Enterica ◽  
Foodborne Pathogen ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Public Health Issue ◽  
Diagnostic Laboratory ◽  
Disk Diffusion Method

Background and Aim: Salmonella enterica is an important foodborne pathogen and is recognized as a major public health issue. The emergence of multidrug-resistant (MDR) S. enterica represents a major challenge for national public health authorities. We investigated the distribution of serovars and antimicrobial resistance of S. enterica isolates from clinical swine samples stored at the Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University from 2016 to 2017. Materials and Methods: Clinical samples were collected and subjected to standard microbiological techniques outlined in the Manual of Clinical Microbiology to identify Salmonella serovars. Susceptibility to antimicrobials was tested by the Kirby–Bauer disk diffusion method using a panel of 14 antimicrobials. Results: A total of 144 Salmonella isolates were identified and the dominant serovar was Salmonella Choleraesuis (66.67%), followed by monophasic Salmonella Typhimurium (18.75%), S. Typhimurium (9.03%), and Rissen (5.56%). The isolates displayed high resistance rates to ampicillin (AMP [100%]), amoxicillin (AX [100%]), tetracycline (TE [100%]), cefotaxime (CTX [89.58%]), ceftriaxone (CRO [87.50%]), chloramphenicol (C [82.64%]), gentamicin (CN [79.17%]), nalidixic acid (NA [72.92%]), and ceftazidime (CAZ [71.53%]). All isolates were MDR, with 29 distinct resistance patterns. The dominant MDR pattern among serovars Choleraesuis and Rissen exhibited resistance to 9 antimicrobials: ( R7-14 AMP-AX-CAZ-CRO-CTX-NA-C-CN-TE). However, all tested isolates were susceptible to AX/ clavulanic acid and fosfomycin. Conclusion: High resistance levels to the third generation of cephalosporins such as CAZ, CRO, and CTX highlight the need for careful and reasonable usage of antimicrobials in animals and humans, especially for S. Choleraesuis infections.

scholarly journals Salmonella contamination, serovars and antimicrobial resistance profiles of cattle slaughtered in South Africa

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Evelyn Madoroba ◽  
Daniel Kapeta ◽  
Awoke K. Gelaw
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
South African ◽  
Environmental Samples ◽  
Salmonella Enteritidis ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Intestinal Contents ◽  
Microbiological Techniques ◽  
Salmonella Serovars

Antimicrobial resistant Salmonella are among the leading causes of foodborne infections. Our aim was to determine Salmonella contamination during cattle slaughter in South African rural abattoirs (n = 23) and environmental samples. Furthermore, antimicrobial resistance patterns of the Salmonella isolates were determined. Samples of cattle faeces (n = 400), carcass sponges (n = 100), intestinal contents (n = 62), hides (n = 67), and water from the abattoirs (n = 75) were investigated for Salmonella species using microbiological techniques and species-specific polymerase chain reaction targeting the invA gene. In total 92 Salmonella species isolates were recovered. The Salmonella mean frequency of occurrence on hides, carcasses, and intestinal contents was 35.37% (n = 81). Eleven faecal samples (2.75%) tested positive for Salmonella. The predominant serovar was Salmonella Enteritidis. Diverse serovars that were identified on carcasses were not necessarily found on the hides and intestinal contents. The inconsistent occurrence of the diverse Salmonella serovars on hides, carcasses, and intestinal contents implies that in addition to carriage on hides and in intestinal contents, other external factors also play an important role regarding carcass contamination. The 92 Salmonella were serotyped and tested for susceptibility towards the following antimicrobials: ampicillin, cefotaxime, enrofloxacin, kanamycin, and oxytetracycline using the disk diffusion method. Most Salmonella (n = 66; 71.7%) isolates were resistant to at least one antimicrobial with highest resistance observed towards oxytetracycline (51.90%), which highlights the need for strict hygiene during slaughter and prudent antimicrobial use during animal production. In conclusion, cattle slaughtered in South African rural abattoirs harbour diverse Salmonella serovars that are resistant to antimicrobials, which could be a public health risk. The findings should assist policymakers with improving implementation of hygienic slaughter of cattle in rural abattoirs, which is paramount from socioeconomic, public health, and epidemiological standpoints.Keywords: Salmonella; Cattle; Rural abattoirs; slaughter; Multidrug resistance; Environmental samples

scholarly journals Occurrence of Escherichia coli producing extended spectrum β-lactamases in food-producing animals

2021 ◽  
Author(s):  
Bence Balázs ◽  
József Bálint Nagy ◽  
Zoltán Tóth ◽  
Fruzsina Nagy ◽  
Sándor Károlyi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Families ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Chain Reactions ◽  
E Coli ◽  
Esbl Gene ◽  
Source Of Infection ◽  
Extended Spectrum ◽  
Esbl Producers

Abstract Multidrug resistance due to the production of extended-spectrum beta-lactamases (ESBLs) is a major problem in human as well as in veterinary medicine. These strains appear in animal and human microbiomes and can be the source of infection both in animal and in human healthcare, in accordance with the One Health theorem. In this study we examined the prevalence of ESBL-producing bacteria in food-producing animals. We collected 100 porcine and 114 poultry samples to examine the prevalence of ESBL producers. Isolates were identified using the MALDI-TOF system and their antibiotic susceptibility was tested using the disk diffusion method. ESBL gene families and phylogroups were detected by polymerase chain reactions. The prevalence of ESBL producers was relatively high in both sample groups: 72 (72.0%) porcine and 39 (34.2%) poultry isolates were ESBL producers. Escherichia coli isolates were chosen for further investigations. The most common ESBL gene was CTX-M-1 (79.3%). Most of the isolates belong to the commensal E. coli phylogroups. The porcine isolates could be divided into three phylogroups, while the distribution of the poultry isolates was more varied. In summary, ESBL-producing bacteria are prevalent in the faecal samples of the examined food-producing animals, with a dominance of the CTX-M-1 group enzymes and commensal E. coli phylogroups.

scholarly journals Occurrence and Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Dairy Cattle, Milk, and Farm Environments in Peninsular Malaysia

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1007
Author(s):  
Emelia Aini Kamaruzzaman ◽  
Saleha Abdul Aziz ◽  
Asinamai Athliamai Bitrus ◽  
Zunita Zakaria ◽  
Latiffah Hassan
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cattle ◽  
One Health ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Extended Spectrum ◽  
Antimicrobial Resistance Genes

The emergence and spread of antimicrobial resistance genes and resistant bacteria do not recognize animal, human, or geographic boundaries. Addressing this threat requires a multidisciplinary approach involving human, animal, and environmental health (One Health) sectors. This is because antimicrobial agents used in veterinary medicine have been reported to be the same or similar to those in human medicine use. Extended-spectrum β-lactamase (ESBL) E. coli is a growing public health problem worldwide, and the agri-food industry is increasingly becoming a source of clinically important ESBL bacteria. Accordingly, the aim of this study was to investigate the occurrence and characteristics of ESBL-producing E. coli from dairy cattle, milk, and the farm environment. E. coli isolates were identified by their 16sRNA, and their ESBL production was confirmed using ESBL–CHROMagar media and the double disk diffusion method. Genotypes of ESBL producers were characterized using multiplex polymerase chain reaction (mPCR) assay. It was found that 18 (4.8%) of the total samples were positive for ESBL-producing E. coli. Of these, 66.7% were from milk, and 27.8% and 5.5% were from the farm environment and faecal samples, respectively. Predominant ESBL genotypes identified were a combination of both TEM and CTX-M in eight out of 18 (44.4%) isolates. Four (22.2%) isolates produced the CTX-M gene, two (11.1%) isolates produced the TEM gene, and four (22.2%) remaining isolates produced the ESBL genes other than TEM, SHV, CTX-M, and OXA. The SHV and OXA gene were not detected in all 18 isolates. In all, there were four profiles of genetic similarity. The occurrence of these genotypes in indicator organisms from dairy cattle, milk, and the farm environment further re-enforced the potential of food-animals as sources of ESBL-producing E. coli infection in humans via the food chain. Thus, there is the need for the adoption of a tripartite One Health approach in surveillance and monitoring to control antimicrobial resistance.

Prevalence and Molecular Characterization of Antimicrobial Resistance in Escherichia coli Isolated from Raw Milk and Raw Milk Cheese in Egypt

2018 ◽  
Vol 81 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Rabee A. Ombarak ◽  
Atsushi Hinenoya ◽  
Abdel-Rahman M. Elbagory ◽  
Shinji Yamasaki
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Raw Milk ◽  
Diffusion Method ◽  
Sequencing Analysis ◽  
Aminoglycoside Resistance ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT The goal of this study was to examine antimicrobial resistance and characterize the implicated genes in 222 isolates of Escherichia coli from 187 samples of raw milk and the two most popular cheeses in Egypt. E. coli isolates were tested for susceptibility to 12 antimicrobials by a disk diffusion method. Among the 222 E. coli isolates, 66 (29.7%) were resistant to one or more antimicrobials, and half of these resistant isolates showed a multidrug resistance phenotype (resistance to at least three different drug classes). The resistance traits were observed to tetracycline (27.5%), ampicillin (18.9%), streptomycin (18.5%), sulfamethoxazole-trimethoprim (11.3%), cefotaxime (4.5%), kanamycin (4.1%), ceftazidime (3.6%), chloramphenicol (2.3%), nalidixic acid (1.8%), and ciprofloxacin (1.4%). No resistance to fosfomycin and imipenem was observed. Tetracycline resistance genes tetA, tetB, and tetD were detected in 53 isolates, 9 isolates, and 1 isolate, respectively, but tetC was not detected. Aminoglycoside resistance genes strA, strB, aadA, and aphA1 were detected in 41, 41, 11, and 9 isolates, respectively. Sulfonamide resistance genes sul1, sul2, and sul3 were detected in 7, 25, and 3 isolates, respectively. Of 42 ampicillin-resistant isolates, blaTEM, blaCTX-M, and blaSHV were detected in 40, 9, and 3 isolates, respectively, and 10 (23.8%) ampicillin-resistant isolates were found to produce extended-spectrum β-lactamase. Each bla gene of extended-spectrum β-lactamase–producing E. coli was further subtyped to be blaCTX-M-15, blaCTX-M-104, blaTEM-1, and blaSHV-12. The class 1 integron was also detected in 28 resistant isolates, and three different patterns were obtained by PCR-restriction fragment length polymorphism. Sequencing analysis of the variable region revealed that four isolates had dfrA12/orfF/aadA2, two had aadA22, and one had dfrA1/aadA1. These data suggest that antimicrobial-resistant E. coli are widely distributed in the milk production and processing environment in Egypt and may play a role in dissemination of antimicrobial resistance to other pathogenic and commensal bacteria.

scholarly journals Occurrence and Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Dairy Cattle, Milk and Farm Environment in Peninsular Malaysia

2020 ◽  
Author(s):  
Emelia Aini Kamaruzzaman ◽  
Saleha Abdul-Aziz ◽  
Asinamai Athliamai Bitrus ◽  
Zunita Zakaria ◽  
Latiffah Hassan
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cattle ◽  
One Health ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Extended Spectrum ◽  
Antimicrobial Resistance Genes

.The emergence and spread of antimicrobial resistance genes and resistant bacteria does not recognized animal, human or geographic borders. Addressing this threat requires a combination of multidisciplinary approach involving human, animal and environmental health (One Health). Because antimicrobial agents used in veterinary medicine maybe the same or like those in human medicine. Extended-spectrum beta lactamase (ESBL) E. coli is a growing public health problem worldwide, and the Agri-Food industry is constantly becoming sources of clinically important ESBL bacteria. Accordingly, the aim of this study was to investigate the occurrence and characteristics of ESBL-producing E. coli from dairy cattle, milk, and the farm environment. E. coli isolates were identified by their 16sRNA and their ESBL production was confirmed by ESBL-CHROMagar media and double disk diffusion method. Genotypes of ESBL producers were characterised using mPCR assay. Among the examined samples, 18(4.8 %) were positive for ESBL-producing E. coli. Of these, 66.7% were from milk, 27.8% and 5.5% were from farm environment and faecal samples respectively. Predominant ESBL Genotype identified were a combination of both TEM and CTX-M in eight out of 18 (44.4%) isolates. Four (22.2%) isolates produced CTX-M gene, two (11.1%) isolates produced TEM gene and four (22.2%) remaining isolates produced ESBL genes other than TEM, SHV, CTX-M and OXA. The SHV and OXA gene were not detected in all 18 isolates. The occurrence of these genotype in indicator organisms from dairy cattle, milk, and farm environment further re-enforced the potentials of food-animals as sources of infection for humans via the food chain. Thus, consolidating the need for the adoption of tripartite One Health approach in surveillance and monitoring antimicrobial resistance

Antimicrobial Resistance Surveillance of Klebsiella Spp. Isolated from the First Bethune Hospital

2011 ◽  
Vol 268-270 ◽  
pp. 1954-1956
Author(s):  
Jian Cheng Xu ◽  
Jun Han ◽  
Yan Ling Yang ◽  
Qi Zhou
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Nosocomial Infections ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Opportunistic Pathogens ◽  
Disk Diffusion Method ◽  
Antimicrobial Resistance Surveillance ◽  
Extended Spectrum ◽  
Multiple Antibiotics

Klebsiella spp.are opportunistic pathogens which frequently cause community and nosocomial infections.The present study aimed to evaluate the antibiotic resistance profiles of Klebsiella spp. isolated from the First Bethune Hospital. Disk diffusion method was used to study the antimicrobial resistance. The data were analyzed by WHONET 5 software according to Clinical and Laboratory Standards Institute (CLSI). The majority of 3149 strains of Klebsiella spp. were collected from sputum（1890, 60.0%），blood（378, 12.0%） and secretions（315, 10.0%）. The percentage of extended-spectrum β-lactamases (ESBLs) in Klebsiellaspp. was 53.8% (1694/3149). Klebsiella spp. strains were frequently resistant to multiple antibiotics. Antimicrobial resistance of ESBL-producing Klebsiella spp. was more serious than that of non-ESBL-producing isolates. The results suggest that surveillance of antimicrobial resistance in Klebsiella spp. is necessary.

scholarly journals Detection of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Production in Klebsiella Pneumoniae Strains from Infected Neonates

2002 ◽  
Vol 30 (4) ◽  
pp. 445-448 ◽  
Author(s):  
E Aktas ◽  
N Yigit ◽  
H Yazgi ◽  
A Ayyildiz
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Neonatal Intensive Care ◽  
Clinical Laboratory ◽  
Significant Risk ◽  
University Hospital ◽  
Diffusion Method ◽  
National Committee ◽  
Disk Diffusion Method ◽  
Extended Spectrum

The present study was designed to determine the antimicrobial resistance and extended-spectrum β-lactamase (ESBL) activities of Klebsiella pneumoniae strains isolated from the neonatal intensive care unit of Atatürk University Hospital, Erzurum, Turkey. Antibiotic susceptibility of 40 isolates was detected by the standard disk diffusion method according to the National Committee for Clinical Laboratory Standards Guidelines. The double-disk synergy method was used to determine ESBL activity, which is associated with resistance to β-lactam antibiotics. Twenty-four (60%) of 40 K. pneumoniae strains were found to produce ESBL. Of the antibiotics tested, meropenem was found to be the most effective (100%), and ampicillin the least effective (0%). With the increasing incidence of antimicrobial resistance, which poses a clinically significant risk to vulnerable patients, it is important that clinical microbiology laboratories have accurate and timely information concerning the strains of bacteria present to enable them to predict which antibiotics are likely to be effective in treating the infections they may cause.

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