AmpC- and Extended-Spectrum β-Lactamase–Producing Enterobacteriaceae Detected in Fresh Produce in Central Ohio

2021 ◽  
Vol 84 (5) ◽  
pp. 920-925
Author(s):  
ELIZABETH PARKER ◽  
AMY ALBERS ◽  
DIXIE MOLLENKOPF ◽  
DANIELA KOREC ◽  
DIMITRIA MATHYS ◽  
...  
Keyword(s):  
Fresh Produce ◽  
Geometric Mean ◽  
Farmers Markets ◽  
Grocery Stores ◽  
Salmonella Spp ◽  
Leafy Greens ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Salad Vegetables ◽  
Carbapenem Resistant Enterobacteriaceae

ABSTRACT Salad vegetables purchased from farmer's markets and grocery stores in central Ohio during the summers of 2015 and 2016 were tested for the presence of Enterobacteriaceae resistant to extended-spectrum cephalosporins and carbapenems, Salmonella contamination, and coliform bacterial counts. A total of 364 samples were collected from 36 farmers' markets and 33 grocery stores. Using selective media, we found 23 (6.3%) samples that produced Enterobacteriaceae expressing an AmpC β-lactamase phenotype, with 11 (3.0%) confirmed to contain blaCMY and 6 (1.6%) that produced Enterobacteriaceae with an extended-spectrum β-lactamase (ESBL) phenotype, 1 (0.3%) of which was confirmed to contain blaCTX-M. All blaCMY and blaCTX-M strains were isolated from leafy greens. No Salmonella spp. or carbapenem-resistant Enterobacteriaceae were recovered from fresh produce samples. Adjusting for year, the geometric mean coliform count differed (P < 0.05) between produce types, with the count in tomatoes (15 CFU/mL) being lower than those in cucumbers (77.4 CFU/mL) and leafy greens (75.0 CFU/mL). The coliform counts also differed (P < 0.05) between years, with 19.6 CFU/mL in 2015 and 96.0 CFU/mL in 2016. There was no difference in coliform counts or the prevalences of Enterobacteriaceae expressing AmpC or ESBL phenotypes in produce purchased from farmers' markets and grocery stores. HIGHLIGHTS

scholarly journals 1591. Antibiotic Utilization Trends in Veterans Affairs (VA) Patients with Carbapenem Resistant Enterobacteriaceae (CRE) Infections

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S792-S792
Author(s):  
Alfredo Traversa ◽  
Linda Poggensee ◽  
Geneva M Wilson ◽  
Katie J Suda ◽  
Charlesnika T Evans ◽  
...  
Keyword(s):  
Treatment Options ◽  
Blood Stream ◽  
Chi Square ◽  
Blood Stream Infections ◽  
Exact Test ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
New Antibiotics ◽  
Chi Square Test ◽  
Carbapenem Resistant Enterobacteriaceae

Abstract Background Carbapenem-resistant Enterobacteriaceae (CRE) are classified as an “urgent threat” to public health. Historically, colistin and tigecycline had been considered the drugs of choice for CRE infections, while other agents such as aminoglycosides and carbapenems had been used as adjunctive therapy. However, the FDA approval of ceftazidime-avibactam in 2015, meropenem-vaborbactam in 2017, and plazomicin in 2018 has expanded treatment options. Our purpose was to assess trends in CRE treatment for “new” antibiotics (ceftazidime-avibactam, meropenem-vaborbactam, plazomicin) as compared with other antibiotics with CRE activity. Methods This was a retrospective cohort study describing treatment of CRE blood stream infections (BSI) across 134 VA facilities from 2012-2018. Patients were censored at their first positive blood culture with CRE. Categorical data was assessed with a Fisher’s exact test or chi-square test. Trends test and logistic regression were used to describe changes in CRE treatment over time. Results 724 patients with positive blood cultures for CRE were identified during the study period. Most patients were male (94%), white (32%) or Hispanic (38%), and the mean age was 71.5+11.9. Of those patients that received antibiotics (N=697), 53.4% carbapenems, 40.3% received aminoglycosides, 39.3% received polymyxins, 32.9% penicillins, 32.6% extended spectrum cephalosporins, 26.1% fluoroquinolones, 11.6% ceftazidime/avibactam, and 0.4% ceftolazone/tazobactam. Over the study period, there was decreased utilization of aminoglycosides (P < 0.0026) and colistin (P< 0.002) and increases in extended spectrum cephalosporins (P < 0.001) and ceftazidime/avibactam (P < 0.001). Conclusion Utilization of “older” agents such as aminoglycosides and polymyxins for the treatment of CRE blood stream infections is decreasing in the VA. Treating CRE with ceftazidime/avibactam, a newly approved antibiotic, and extended spectrum cephalosporins are increasing. Disclosures All Authors: No reported disclosures

scholarly journals (148) A Lab Exercise to Enhance a Student's Understanding of Botany in Everyday Life using Fresh Produce

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1045A-1045
Author(s):  
Melinda McVey McCluskey
Keyword(s):  
Everyday Life ◽  
Fruits And Vegetables ◽  
Fresh Produce ◽  
Plant Part ◽  
Farmers Markets ◽  
Grocery Stores ◽  
Plant Parts ◽  
Star Fruit ◽  
Vegetables And Fruits ◽  
Exotic Fruits

Students are often unable to relate the vegetables and fruits consumed as a snack or part of a meal to the plant parts discussed in botany class. Therefore, an exercise was developed for an introductory horticulture course to increase a student's awareness of botany in everyday life. Fresh produce was brought in from local gardens, grocery stores, or farmers markets. Vegetables and fruits were selected that are consumed for their roots, stems, leaves, flower, fruit, and seeds. As each vegetable or fruit was introduced, students named the plant and plant part. As each part was identified it was “dissected” to show the taxonomic features. The different fruit types, i.e., berry, hesperidium, pepo, drupe, and pome were explained. Students were encouraged to taste all vegetables and fruits as they were prepared. Most students sampled the produce as it was passed around the group. Students easily recognized much of the produce, i.e., carrots, asparagus, tomatoes, peas, oranges, and broccoli. The second part of the exercise exposed students to vegetables and fruits that were unfamiliar. Most of the students had little exposure to the more exotic fruits and vegetables that are now available. New vegetables and fruits that students said they would add to their diet include jicama, pomegranate, and star fruit.

scholarly journals Most Preferred Market Venues for Locally Grown Fresh Produce among Online Shoppers

2017 ◽  
Vol 9 (10) ◽  
pp. 26 ◽  
Author(s):  
Jean D. Gumirakiza ◽  
Sarah M. VanZee
Keyword(s):  
Fresh Produce ◽  
Marketing Strategies ◽  
Farmers Markets ◽  
Grocery Stores ◽  
Relative Probability ◽  
Future Studies ◽  
Online Purchases ◽  
Online Shoppers ◽  
Locally Grown ◽  
On Farm

This paper examines the most preferred market venues online shoppers use when purchasing locally/regionally grown fresh produce. We surveyed 1,205 consumers who made online purchases at least twice within six months prior to participating in this study. We call these consumers “online shoppers”. The study was conducted in the Southern region of the U.S. We collected data using Qualtrics actively managed market research panels and social media such as Facebook, and Twitter. Results indicate that grocery stores are the most preferred market venue online shoppers use to purchase locally/regionally grown fresh produce with a relative probability of 44 percent. Farmers’ markets are the second most preferred market venues with a likelihood of 33 percent. The third market venue is on-farm/CSA programs with relative probability of seven percent. Online markets are currently the fourth most frequented with a likelihood of five percent. 11 percent have no particular most preferred market venue. This analysis is significant to fresh produce growers and marketers. Results suggest new marketing strategies to conveniently make fresh produce accessible among online shoppers. Furthermore, this study is useful for future studies with interests in explaining the preferred market venues for local/regional fresh produce among online shoppers.

scholarly journals 1351. In vitro Activity of Cefiderocol (S-649266), a Siderophore Cephalosporin, Against Enterobacteriaceae With Defined Extended-Spectrum Β-Lactamases and Carbapenemases

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S413-S414 ◽  
Author(s):  
Michael R Jacobs ◽  
Ayman M Abdelhamed ◽  
Caryn E Good ◽  
Daniel D Rhoads ◽  
Kristine M Hujer ◽  
...  
Keyword(s):  
Clinical Utility ◽  
Growth Control ◽  
Multidrug Resistant ◽  
Federally Funded ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Mueller Hinton ◽  
Carbapenem Resistant Enterobacteriaceae ◽  
Research Grant

Abstract Background Cefiderocol is a novel siderophore cephalosporin targeted for activity against carbapenem and multidrug-resistant Gram-negative species, including extended-spectrum β-lactamase (ESBL) and carbapenemase-producing strains. The Consortium on Resistance Against Carbapenems in Klebsiella and other Enterobacteriaceae (CRACKLE) is a federally funded, prospective multi-center consortium of 20 hospitals from nine US healthcare systems to track carbapenem-resistant Enterobacteriaceae. Methods Minimum inhibitory concentrations (MICs) of cefiderocol and meropenem were determined by broth microdilution according to current CLSI guidelines. Cefiderocol was tested in iron-depleted cation-adjusted Mueller–Hinton (MH) broth, meropenem was tested in cation-adjusted MH broth. Cefiderocol MICs were read as the first drug well in which the growth was significantly reduced (i.e., a button of <1 mm or light/faint turbidity) relative to the growth observed in the growth control well containing the same medium. Trailing endpoints were disregarded. Isolates tested included 35 Escherichia coli, five Enterobacter/Citrobacter group, and 794 Klebsiella pneumoniae. Isolates had characterized β-lactamases including TEM, SHV, and CTX-M ESBLs and KPC, NDM, and OXA carbapenemases. Results Cefiderocol MICs ranged from ≤0.03 to >64 mg/L, with overall MIC50 of 0.5 mg/L and MIC90 of 4 mg/L (table). MIC90 value (≤0.03 mg/L) was lowest against isolates with no ESBLs or carbapenemases. MIC90 was 1 mg/L for OXA and TEM/SHV groups, 2–4 mg/L for KPC-3 groups and 8 mg/L for NDM and KPC-2 groups. Conclusion Compared with isolates without ESBLs and carbapenemases, cefiderocol shows higher MICs against isolates with ESBLs, including TEM, SHV, and CTX-M and carbapenemases including KPC, NDM, and OXA. The clinical utility of cefiderocol against ESBL and carbapenemase-producing Enterobacteriaceae is dependent on the pharmacokinetic and pharmacodynamic properties of cefiderocol. Disclosures M. R. Jacobs, Achaogen: Investigator, Research grant. Shionogi: Investigator, Research grant. S. S. Richter, bioMerieux: Grant Investigator, Research grant. BD Diagnostics: Grant Investigator, Research grant. Roche: Grant Investigator, Research grant. Hologic: Grant Investigator, Research grant. Diasorin: Grant Investigator, Research grant. Accelerate: Grant Investigator, Research grant. Biofire: Grant Investigator, Research grant. D. Van Duin, Shionogi: Scientific Advisor, Consulting fee. achaogen: Scientific Advisor, Consulting fee. Allergan: Scientific Advisor, Consulting fee. Astellas: Scientific Advisor, Consulting fee. Neumedicine: Consultant, Consulting fee. T2 Biosystems: Scientific Advisor, Consulting fee. Roche: Scientific Advisor, Consulting fee.

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