scholarly journals EFICIÊNCIA DE DETERGENTES NA REMOÇÃO DE BIOFILMES DE CEPA DE ESCHERICHIA COLI EM SUPERFÍCIE DE AÇO INOXIDÁVEL

2020 ◽  
Vol 57 (4) ◽  
pp. 46-56
Author(s):  
Giovana Garcia Fabiano ◽  
◽  
Raul Gomes Aguera ◽  
Daniela Biral Do Prado ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aisi 304 ◽  
E Coli ◽  
Mueller Hinton

Escherichia coli é um patógeno associado a doenças transmitidas por alimentos. E. coli pode estar presente na indústria de alimentos através de biofilmes em condições higiênicas inadequadas. O objetivo deste estudo foi avaliar a eficiência de detergentes na remoção de biofilmes de E. coli em superfície de aço inoxidável. Os cupons de aço inoxidável AISI 304, acabamento número 4 (dimensões de 8 mm x 8 mm x 1 mm) foram imersos assepticamente em microtubos contendo 1mL de caldo Mueller Hinton (MH) contaminado com 105 UFC/mL de E.coli e incubados a 35 °C por 24h para formação do biofilme. Após, cada cupom foi submetido a procedimentos de higienização: limpeza com detergente enzimático por 5 min a temperatura de 40 °C e limpeza com detergente alcalino a 1% por 5 min a temperatura ambiente. Após as etapas de limpeza, cada cupom foi transferido para microtubo contendo 1 mL de solução salina a 0,85% e submetidos a agitação por 2 min para desprendimento do biofilme. Após, foram realizadas as diluições seriadas e o plaqueamento em ágar (MH) para a quantificação das células. Os cupons do controle não receberam agentes de limpeza e suas contagens foram utilizadas para o cálculo do número de reduções decimais devido aos processos de higienização. O tratamento com o detergente enzimático apresentou uma redução de 1,31 log UFC/cm2 . O detergente alcalino reduziu (3,70 log UFC/cm2 ) as contagens de E.coli da superfície de aço inoxidável. Nenhum método de higienização testado removeu totalmente o biofilme microbiano da superfície de aço inoxidável.

Escherichia coli ATCC 35218 as a Quality Control Isolate for Susceptibility Testing of Haemophilus influenzae with Haemophilus Test Medium

1999 ◽  
Vol 43 (2) ◽  
pp. 283-286 ◽  
Author(s):  
D. L. Butler ◽  
C. J. Jakielaszek ◽  
L. A. Miller ◽  
J. A. Poupard
Keyword(s):  
Escherichia Coli ◽  
Quality Control ◽  
Clavulanic Acid ◽  
Susceptibility Testing ◽  
Test Medium ◽  
E Coli ◽  
Mueller Hinton ◽  
Quality Control Testing ◽  
Amoxicillin Clavulanic Acid ◽  
Lactamase Inhibitor

ABSTRACT Current National Committee for Clinical Laboratory Standards (NCCLS) susceptibility guidelines for quality control testing withHaemophilus influenzae do not include a β-lactamase-producing strain that could detect the deterioration of the β-lactamase inhibitor components of amoxicillin-clavulanic acid, ampicillin-sulbactam, and piperacillin-tazobactam. The objective of the study was to determine if comparable quality control results forEscherichia coli ATCC 35218, a β-lactamase-producing strain, would be produced for the three β-lactam–β-lactamase inhibitor agents with Haemophilus test medium and Mueller-Hinton medium. The criteria used in this study to determine if Haemophilus test medium was acceptable for quality control testing of E. coli ATCC 35218 was that 100% of the results obtained with an antimicrobial agent-methodology combination needed to be within the acceptable NCCLS ranges established with Mueller-Hinton medium. The MIC testing results obtained by the broth microdilution and E-test methods with amoxicillin-clavulanic acid and piperacillin-tazobactam were all within the NCCLS ranges; however, the results obtained with ampicillin-sulbactam by both methods were not within the NCCLS ranges. Acceptable results were obtained by the disk diffusion methodology with ampicillin-sulbactam and piperacillin-tazobactam but not with amoxicillin-clavulanic acid. When performing susceptibility testing with H. influenzae with the β-lactam–β-lactamase inhibitors, in addition to quality control testing with H. influenzae ATCC 49247, testing of E. coli ATCC 35218 on Haemophilus test medium is an effective way to monitor the β-lactamase inhibitors in some antimicrobial agent-methodology combinations.

scholarly journals PSV-41 Antibacterial activity of ferulic acid and sodium chlorate against Escherichia coli F18 and K88 during incubations with porcine fecal bacteria

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 296-297 ◽  
Author(s):  
Claudio Arzola ◽  
Elizabeth Latham ◽  
Robin Anderson ◽  
Jaime Salinas-Chavira ◽  
Yamicela Castillo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ferulic Acid ◽  
Sodium Chlorate ◽  
Fecal Bacteria ◽  
Aerobic Incubation ◽  
E Coli ◽  
Mueller Hinton ◽  
Fecal Suspension ◽  
Porcine Feces ◽  
Mueller Hinton Broth

Abstract The influence of ferulic acid (FA) and sodium chlorate (SC) was evaluated in two trials on the growth of Escherichia coli F18 and K88 (F18 and K88) incubated with porcine fecal bacteria. Treatments were 2 levels of FA (0 and 5 mg/mL) and 2 levels of SC (0 and 10 mM/mL). In trial one, ½-strength Mueller Hinton broth mixed with porcine feces (0.5% w/v) was inoculated with a novobiocin and naladixic acid resistant F18-strain. This fecal suspension was transferred to tubes (3/treatment) and anaerobically incubated at 39 oC for enumeration at 0, 6 and 24 h using MacConkey agar supplemented with novobiocin and naladixic acid with aerobic incubation at 37 oC. An interaction (FA x SC) at 6 and 24 h was observed (P < 0.01). At 6 h of incubation, SC alone or combined with FA had the lowest counts (P < 0.05); FA alone was lower than control but higher than SC or SC+FA (P < 0.05). At 24 h, FA alone or combined with SC had the lowest counts (P < 0.05); SC was lower than control but higher than FA or SC+FA (P < 0.05). In trial 2 were used the same procedures of trial 1, except that K88 was used. There was an interaction at 6 h (P < 0.01) where the lowest counts were in FA+SC (P < 0.05). SC alone or FA alone were lower than control but higher than SC+FA (P < 0.05). There was no interaction at 24 h (P = 0.16), where FA reduced the K88 counts (P < 0.01), however it was not affected by SC (P = 0.12). In conclusion, SC reduced E. coli counts; however, at 24 h of incubation greater reductions were observed when FA alone or combined with SC was added into the incubation fluid with porcine feces.

scholarly journals PSV-40 Antibacterial activity of sodium chlorate and essential oils against Escherichia coli (F18 and K88) during incubations with porcine fecal bacteria

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 295-296
Author(s):  
Jaime Salinas-Chavira ◽  
Robin Anderson ◽  
Elizabeth Latham ◽  
Rafael Cabrera ◽  
Yamicela Castillo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Sodium Chlorate ◽  
Fecal Bacteria ◽  
Cinnamon Oil ◽  
E Coli ◽  
Mueller Hinton ◽  
Fecal Suspension ◽  
Porcine Feces

Abstract In two trials was evaluated the influence of sodium chlorate (SC) and essential oils (EO) on the growth of Escherichia coli F18 and K88 (F18 and K88) incubated with porcine fecal bacteria. The treatments were 2 levels of SC (0 and 10 mM/mL) and 2 levels of Activo® (0 and 1.5%; vol/vol). Activo® (EW Nutrition, Des Moines, IA) is a blend of oregano oil and cinnamon oil (EO) with water and citric acid. In trial one, ½-strength Mueller Hinton broth mixed with porcine feces (0.5% w/vol) was inoculated with a novobiocin and naladixic acid resistant F18-strain. This fecal suspension was transferred to tubes (3/treatment) and anaerobically incubated at 39 oC for enumeration at 0, 6 and 24 h using MacConkey agar supplemented with novobiocin and naladixic acid with aerobic incubation at 37 oC. An interaction (EO x SC) at 6 and 24 was observed (P < 0.01). At 6 and 24 h, EO alone or combined with SC had the lowest counts of F18 (P < 0.05); SC alone had lower counts of F18 than control (P < 0.05). In trial 2 were used the same procedures of trial 1, except that K88 was inoculated in the porcine fecal suspension. There was an interaction at 6 h (P < 0.01) where EO had the lowest counts of K88. The control showed the highest counts of K88 (P < 0.05). There was no interaction at 24 h (P = 0.14). The counts of K88 were reduced by EO (P < 0.01), however the counts were not affected by SC (P = 0.14). It was concluded that SC reduced the counts of E. coli F18, but it had minimal effect on E. coli K88 in the challenged porcine feces; essential oils were effective to reduce the pathogenic bacteria in the porcine feces.

scholarly journals AVALIAÇÃO DA ATIVIDADE ÓLEOS ESSENCIAIS DE Thimus vulgaris (TOMILHO), Syzygium aromaticum (CRAVO-DA-INDIA) E Rosmarinus officinalis (ALECRIM) E DOS CONSERVANTES BENZOATO DE SÓDIO E SORBATO DE POTÁSSIO EM Escherichia coli E Staphylococcus aureus

2015 ◽  
Vol 33 (1) ◽  
Author(s):  
Angela Aparecida Da Silva ◽  
Márcia Maria Dos Anjos ◽  
Suelen Pereira Ruiz ◽  
Lucimara Bergamo Panice ◽  
Jane Martha Graton Mikcha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Rosmarinus Officinalis ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Neste trabalho foi avaliada a ação dos óleos essenciais de Thymus vulgaris (tomilho), Syzygium aromaticum (cravo-da-índia) e Rosmarinus officinalis (alecrim) e dos conservantes benzoato de sódio e sorbato de potássio como agentes antimicrobianos. As cepas de Staphylococcus aureus (ATCC 25923) e Escherichia coli (ATCC 25922) foram utilizadas no teste de susceptibilidade antimicrobiana usando-se a técnica de microdiluição em microplaca de 96 poços para avaliação da Concentração Inibitória Mínima (CIM) e, posteriormente, subcultivo em Mueller Hinton Agar para avaliaçãoda Concentração Bactericida Mínima (CBM). As concentrações dos óleos e conservantes sintéticos testados variaram de 15,6 a 1000μg/mL. As microdiluições utilizando inóculos bacterianos nas concentrações de 104 UFC/mL foram incubadas a 37ºC/24h. As CIM para os óleos essenciais de cravo, tomilho e alecrim foram de 550, 650 e >1000μg/mL para E. coli e 550, 800 e 1000μg/mL para S. aureus, respectivamente. No entanto, a ação bactericida dos óleos essenciais do cravo-da-índia e do tomilho foi encontrada apenas em relação a E. coli, na concentração de 550 e 850μg/mL, respectivamente. Para os dois conservantes sintéticos testados, a CIM foi >1000μg/mL, portanto não apresentaram atividade antibacteriana contra os microrganismos testados. Trabalhos futuros deverão ser realizados para verifi car a efi ciência dos antimicrobianos naturais anteriormente citados e para avaliar a possibilidade de serem utilizados na indústria de alimentos.   

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SIRSAK (Annonae muricata L.) DENGAN METODE DIFUSI AGAR CAKRAM TERHADAP Escherichia coli

2020 ◽  
Vol 1 (1) ◽  
pp. 15-19
Author(s):  
I Made Agus Sunadi Putra
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Diare biasanya disebabkan oleh bakteri Eschericia coli yang terkontaminasi melalui makanan dan minuman yang terkontaminasi bakteri tersebut melalui lalat. Beberapa masyarakat menggunakan daun sirsak sebagai antidiare. Banyak kandungan yang terdapat dalam daun sirsak ini saponin, tanin, alkaloid, dan flavonoid, yang mana senyawa ini dapat berfungsi sebagai desinfektan-antiseptik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol daun sirsak (Annonae muricatae L.) terhadap daya hambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan isolat bakteri E. coli ATCC 25922 yang diperoleh dari Laboratorium Mikrobiologi Fakultas Kedokteran, Universitas Udayana. Hasil perasan daun sirsak berasal dari 100 gram daun sirsak yang dihaluskan kemudian ditambah dengan etanol, maserasi kemudian diperas. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 95%. Konsentrasi ekstrak kental daun sirsak yang digunakan adalah 50%, 80%, 100% dan kontrol positif tetrasiklin. Pengujian aktivitas antibakteri menggunakan metode difusi agar (difusi Kirby dan Bauer yang dimodifikasi) dengan cara cakram. Media yang digunakan adalah Mueller Hinton Agar (MHA). Hasilnya pada konsentrasi 50%, 80% dan 100% tidak menunjukkan zona hambat terhadap bakteri Eschericia coli.

scholarly journals Emergence of high multidrug-resistant Escherichia coli isolates from neonates with infection

2020 ◽  
Author(s):  
Dan Wu ◽  
Yijun Ding ◽  
Jinjing Zhang ◽  
Kaihu Yao ◽  
Wei Gao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Clavulanic Acid ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Amoxicillin Clavulanic Acid

Abstract Background Escherichia coli (E.coli) rank one of the most common pathogens that can cause neonatal infections. The emergence of antibiotic-resistant bacteria is a major cause of treatment failure in newborn with infection. The purpose of this study was to describe the antibiotic resistance and multidrug-resistance of E.coli isolated from neonates with infection.Methods The antimicrobial susceptibility testing of the E. coli strains to selected antibiotics was assessed with the E-test technique on the Mueller-Hinton agar. The antimicrobials tests were included ceftazidime, cefuroxime, cefatriaxone, amoxicillin, amoxicillin-clavulanic acid, cefoperazone - sulbactam, meropenem, gentamicin, ciprofloxacin and sulfonamides. The minimal inhibitory concerntration (MIC) values of the antimicrobial agents selected for this study was determined by an agar dilution technique on Mueller-Hinton agar according to the Clinical and Laboratory Standards Institute recommendations. Results A total of 100 E. coli strains was isolated from phlegm (n = 78), blood (n = 10), cerebrospinal fluid (n = 5), and umbilical discharge (n = 7) of neonates hospitalized at Beijing Children’s Hospital. The highest resistance rate of E.coli was found in amoxicillin at 85%, followed by cefuroxime 65%, and cefatriaxone 60%, respectively. 6% and 5% of all isolates were resistant to amoxicillin/clavulanic acid and cefoperazone -sulbactam merely. The resistance rates to ceftazidime, gentamicin, ciprofloxacin and sulfonamides were 31%, 20%, 33%, 47%, respectively. All the isolates were susceptible to meropenem. Multidrug resistance was defined in E.coli as resistance to at least three antibiotic families. About 26% (26/100) of all the E.coli isolates were multidrug-resistant. The detection rate of ESBL-Producing E. coli was 55%. The rate in E. coli isolates from phlegm was higher than aseptic humoral. The difference was statistically significant (P < 0.05). It is worth noting that the majority of the isolates were also resistant to non-β-lactam antimicrobial agents, but the resistant rates were significantly lower than extended-spectrum β-lactamases.Conclusions: Multi-drug-resistant E.coli has become a thorny problem in clinical treatment. It is necessary to monitor E. coli resistance.

scholarly journals Eficacia antimicrobiana del aceite esencial de eucalipto (Eucalyptus spp) sobre cepas de Escherichia coli y Staphylococcus aureus subsp. aureus

2019 ◽  
Vol 30 (2) ◽  
pp. 932-938
Author(s):  
Mayra Montero-Recalde ◽  
María José Morocho-Núñez ◽  
Diana Avilés-Esquivel ◽  
Ángela Carrasco-Cando ◽  
Ramiro Erazo-Gutierrez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
E Coli ◽  
Mueller Hinton

El objetivo del estudio fue evaluar el efecto antimicrobiano in vitro del aceite esencial de eucalipto (Eucaliptus spp) sobre Escherichia coli ATCC® 11229 y Staphylococcus aureus subsp. aureus ATCC® 25904. Se evaluaron concentraciones al 30, 60 y 90% en dilución en etanol al 96.8%. Se determinó la Concentración Mínima Inhibitoria (CMI) mediante el método de microdilución en caldo. El inóculo bacteriano se estandarizó al 0.5 de la escala de MacFarland en espectofotómetro, obteniendo como resultado que las concentraciones de 60 y 90% no presentaron turbidez. La Concentración Mínima Bactericida (CMB) determinada en agar Mueller-Hinton se presentó a partir de la concentración del 60% para las dos cepas en estudio. La prueba de sensibilidad antimicrobiana indicó que todas concentraciones presentaron sensibilidad antimicrobiana y que las concentraciones al 30 y 60% no fueron significativamente diferentes, presentando halos de inhibición de 10.25 mm y 10.65 mm para la concentración de 30% para las cepas de Escherichia coli y Staphylococcus aureus subsp. aureus, respectivamente. Por otro lado, los resultados para la cepa S. aureus subsp. aureus muestran que las concentraciones al 60% y 90% formaron halos de mayor diámetro que la cepa de E. coli.

scholarly journals Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

2015 ◽  
Vol 59 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Marcin Weiner ◽  
Hanna Różańska ◽  
Maria Kubajka ◽  
Krzysztof Szulowski ◽  
Monika Krajewska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Meca Gene ◽  
Subclinical Mastitis ◽  
Preliminary Evaluation ◽  
Esbl Production ◽  
E Coli ◽  
Extended Spectrum ◽  
Mueller Hinton ◽  
Genes Encoding ◽  
Peptone Water

Abstract The aim of study was the preliminary evaluation of the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum ß-lactamases (ESBL) - producing Escherichia coli in 650 milk and inflammatory secretions from cows with clinical or subclinical mastitis. One millilitre of the sample was added to Mueller-Hinton broth supplemented with 6.5% NaCl, Tryptone Soya Broth with cefoxitin and aztreonam, and then to MRSA ID agar. Presumptive MRSA colonies were analysed for the presence of mecA gene. Parallel to MRSA identification, the samples were incubated in buffered peptone water, lauryl tryptose broth and McConkey agar supplemented with cefotaxim for ESBL-producing E. coli isolation. These bacteria were identified using API Rapid 32 E and the ability of ESBL production was initially established using disc test D68C and confirmed by MIC technique using Sensititre ESBL plates. The primers (blaCTX, blaTEM, blaSHV, and blaCMY-2-group) for the detection of some of the genes encoding ESBL production were used. The 45 strains of S. aureus with mecA gene and 41 strains of E. coli with blaTEM gene were detected.

scholarly journals Insulin Regulation of Escherichia coli Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1349
Author(s):  
Nina Patel ◽  
Jeremy C. Curtis ◽  
Balbina J. Plotkin
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Fold Increase ◽  
Growth Conditions ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
E Coli ◽  
Biofilm Production ◽  
Mueller Hinton ◽  
Static Conditions

Escherichia coli plays an important role in biofilm formation across a wide array of disease and ecological settings. Insulin can function as an adjuvant in the regulation of biofilm levels. The modulation of insulin-regulated biofilm formation by environmental conditions has not been previously described. In the present study, the effects that various environmental growth conditions and nutrients have on insulin-modulated levels of biofilm production were measured. Micropipette tips were incubated with E. coli ATCC® 25922™ in a Mueller Hinton broth (MH), or a yeast nitrogen base with 1% peptone (YNBP), which was supplemented with glucose, lactose, galactose and/or insulin (Humulin®-R). The incubation conditions included a shaking or static culture, at 23 °C or 37 °C. After incubation, the biofilm production was calculated per CFU. At 23 °C, the presence of insulin increased biofilm formation. The amount of biofilm formation was highest in glucose > galactose >> lactose, while the biofilm levels decreased in shaking cultures, except for galactose (3-fold increase; 0.1% galactose and 20 μU insulin). At 37 °C, regardless of condition, there was more biofilm formation/CFU under static conditions in YNBP than in MH, except for the MH containing galactose. E. coli biofilm formation is influenced by aeration, temperature, and insulin concentration in combination with the available sugars.

scholarly journals Perbandingan Efektivitas Antibakteri Moringa oleifera dan Ziziphus mauritiana dengan Ekstrak Etanol 96% terhadap Escherichia Coli

2020 ◽  
Vol 3 (1) ◽  
pp. 39-46
Author(s):  
Eli Sania ◽  
Sandy Vitria Kurniawan ◽  
Yohanna Angelina
Keyword(s):  
Escherichia Coli ◽  
Moringa Oleifera ◽  
Ziziphus Mauritiana ◽  
E Coli ◽  
Mueller Hinton

Escherichia coli adalah bakteri normal pada pencernaan bagian bawah manusia, namun jika dalam jumlah di atas normal, E. coliakan menimbulkan berbagai penyakit. Penggunaan antibiotik yang tidak tepat dapat menyebabkan resistensi sehingga diperlukan pengobatan alternatif seperti obat-obatan herbal. Tanaman obat yang sudah banyak dieksplorasi sebagai senyawa antibakterial adalah daun kelor(Moringa oleifera)dan daun bidara (Ziziphus mauritiana). Penelitian ini dilakukan untuk melihat perbandingan efektivitas antibakteri Moringa oleifera dan Ziziphus mauritiana dengan pelarut etanol 96% terhadap E.coli.Desain penelitian ini menggunakan studi eksperimental in vitro. Moringa oleifera dan Ziziphus mauritiana diekstrak pada 10 konsentrasi yang berbeda. Metode uji yang digunakan adalah difusi cakram pada agar Mueller-Hinton. Kelompok perlakuan yaitu E. coliATCC 25922, Ciprofloksasin digunakan sebagai kontrol positif dan cakram yang direndam dalam etanol 96% digunakan sebagai kontrol negatif. Rata–rata zona inhibisi ekstrak daun Moringa oleifera adalah 10 mm,sedangkanekstrak daun Ziziphus mauritiana menimbulkan rata–rata zona inhibisi sebesar 14 mm.Rata–rata zona inhibisi dari kontrol positif adalah 33 mm. Kontrol negatif tidak memiliki zona inhibisi. Ekstrak daun Moringa oleifera dapat menginhibisi pertumbuhan bakteri E. coli pada konsentrasi 30% dengan zona inhibisi sebesar 7 mm, dan ekstrak daun Ziziphus mauritianadapat menginhibisi pertumbuhan bakteri E. coli pada konsentrasi 20% dengan zona inhibisi 12 mm. Hal ini membuktikan bahwa Ziziphus mauritiana memiliki kemampuan antibakteri yang lebih efektif daripada Moringa oleifera.

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