scholarly journals Ciprofloxacin loaded castor oil based emulsion: Its antimicrobial, hematotoxicity and soft tissue pathophysiological study in Wister rats

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
A. Bamisaye ◽  
C. O. Eromosele ◽  
E. O. Dare ◽  
O. A. Akinloye
Keyword(s):  
Oral Delivery ◽  
Castor Oil ◽  
Wistar Rats ◽  
Blood Chemistry ◽  
Diffusion Method ◽  
Hematological Indices ◽  
Control Set ◽  
Set Up ◽  
And Control ◽  
Histological Architecture

The emergence of nanocarrier for drug delivery has been flanked with great achievements in the field of nanomedicine. It is however unsafe to assume their toxicological safety. This necessitated examining the toxico-dynamics of ciprofloxacin loaded castor oil based emulsion (COAB+Cp). The emulsions were prepared by mechano-chemical process. The effects of 10 mL per kg of body-weight (BW) of COAB+Cp was estimated in Adult Wistar rats (n=10) for 21 days vis-à-vis a control set up, treated with equal volume of distilled water. The biochemical and haematological parameters were conducted with histopathology of the heart, kidney, spleen and liver. Also, the antimicrobial study on Bacillus. Subtilis (BS) was carried out using the agar well diffusion method. No observable abnormal change in BW gain of both test and control animals. Treatment of animals with COAB+Cp did not cause any observable significant change at P > 0.05 in the blood-chemistry parameters and hematological indices. The photopathological examinations indicates that the histological architecture of vital organs (heart, liver, kidney and spleen) was not compromised in COAB+Cp treated animals. While the recorded value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COAB+Cp corroborated its mean zone of inhibition (ZI) values, signifying a better bioactivity of COAB+Cp compared to Cp on BS. COAB+Cp has no cardiotoxic, hematotoxic and hepatotoxic effects on Wistar rats, with increased potency of the encapsulated Cp thus appears promising as a safe vehicle for oral delivery of ciprofloxacin.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Pittenweem Priory and the conventuality question

2017 ◽  
Vol 68 (1) ◽  
pp. 19-37 ◽  
Author(s):  
Anthony Lodge
Keyword(s):  
Fifteenth Century ◽  
Traditional View ◽  
Archaeological Evidence ◽  
Significant Development ◽  
New Mother ◽  
Set Up ◽  
And Control

Pittenweem Priory began life as the caput manor of a daughter-house established on May Island by Cluniac monks from Reading (c. 1140). After its sale to St Andrews (c. 1280), the priory transferred ashore. While retaining its traditional name, the ‘Priory of May (alias Pittenweem)’ was subsumed within the Augustinian priory of St Andrews. Its prior was elected from among the canons of the new mother house, but it was many decades before a resident community of canons was set up in Pittenweem. The traditional view, based principally on the ‘non-conventual’ status of the priory reiterated in fifteenth-century documents, is that there was ‘no resident community’ before the priorship of Andrew Forman (1495–1515). Archaeological evidence in Pittenweem, however, indicates that James Kennedy had embarked on significant development of the priory fifty years earlier. This suggests that, when the term ‘non-conventual’ is used in documents emanating from Kennedy's successors (Graham and Scheves), we should interpret it more as an assertion of superiority and control than as a description of realities in the priory.

scholarly journals The Combined Effect of Weaning Stress and Immune Activation during Pig Gestation on Serum Cytokine and Analyte Concentrations

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2274
Author(s):  
Haley E. Rymut ◽  
Laurie A. Rund ◽  
Courtni R. Bolt ◽  
Maria B. Villamil ◽  
Bruce R. Southey ◽  
...  
Keyword(s):  
Immune Activation ◽  
Management Practices ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
Blood Chemistry ◽  
Respiratory Syndrome Virus ◽  
Weaning Stress ◽  
And Control ◽  
Metabolic Hormone

Weaning stress can elicit changes in the metabolic, hormone and immune systems of pigs and interact with prolonged disruptions stemming from maternal immune activation (MIA) during gestation. The present study advances the characterization of the combined effects of weaning stress and MIA on blood chemistry, immune and hormone indicators that inform on the health of pigs. Three-week-old female and male offspring of control gilts or gilts infected with the porcine reproductive and respiratory syndrome virus were allocated to weaned or nursed groups. The anion gap and bilirubin profiles suggest that MIA enhances tolerance to the effects of weaning stress. Interleukin 1 beta and interleukin 2 were highest among weaned MIA females, and cortisol was higher among weaned relative to nursed pigs across sexes. Canonical discriminant analysis demonstrated that weaned and nursed pigs have distinct chemistry profiles, whereas MIA and control pigs have distinct cytokine profiles. The results from this study can guide management practices that recognize the effects of the interaction between MIA and weaning stress on the performance and health of pigs.

scholarly journals Effects of a new thyrotropic drug isolated from Potentilla alba on the male reproductive system of rats and offspring development

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lubov V. Krepkova ◽  
Valentina V. Bortnikova ◽  
Aleksandra N. Babenko ◽  
Praskovya G. Mizina ◽  
Vladimir A. Mkhitarov ◽  
...  
Keyword(s):  
Animal Study ◽  
Male Fertility ◽  
Wistar Rats ◽  
Medical Condition ◽  
Adverse Outcomes ◽  
Female Rats ◽  
Childbearing Age ◽  
Offspring Development ◽  
Male Wistar Rats ◽  
And Control

Abstract Background The dysfunction of the thyroid gland is a common medical condition. Nowadays, patients frequently use medicinal herbs as complementary or alternative options to conventional drug treatments. These patients may benefit from treatment of thyroid dysfunctions with Potentilla alba L. preparations. While it has been reported that Potentilla alba preparations have low toxicity, nothing is known about their ability to affect reproductive functions in patients of childbearing age. Methods Male Wistar rats were orally treated with a thyrotrophic botanical drug, standardized Potentilla alba Dry Extract (PADE), at doses 8 and 40 times higher than the median therapeutic dose recommended for the clinical trials, for 60 consecutive days. Male Wistar rats receiving water (H2O) were used as controls. After completing treatment, half of the PADE-treated and control males were used to determine PADE gonadotoxicity, and the remaining half of PADE-treated and control males were mated with intact females. Two female rats were housed with one male for two estrus cycles. PADE effects on fertility and fetal/offspring development were evaluated. Results Herein, we report that oral treatment of male Wistar rats with PADE before mating with intact females instigated marked effects on male reproductive organs. Treatment significantly decreased the motility of the sperm and increased the number of pathological forms of spermatozoa. Additionally, a dose-dependent effect on Leydig cells was observed. However, these PADE effects did not significantly affect male fertility nor fetal and offspring development when PADE-treated males were mated with intact females. Conclusions PADE treatment of male rates negatively affected sperm and testicular Leydig cell morphology. However, these changes did not affect male fertility and offspring development. It is currently not known whether PADE treatment may affect human male fertility and offspring development. Therefore, these results from an animal study need to be confirmed in humans. Results from this animal study can be used to model the exposure-response relationship and adverse outcomes in humans.

scholarly journals Serum C-reactive protein and WBC count in conservatively and operatively managed bacterial spondylodiscitis

2020 ◽  
Vol 29 (1) ◽  
pp. 230949902096829
Author(s):  
Christina van Gerven ◽  
Kevin Eid ◽  
Tobias Krüger ◽  
Michael Fell ◽  
Daniel Kendoff ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Serum Levels ◽  
Blood Chemistry ◽  
Nonoperative Treatment ◽  
C Reactive Protein ◽  
Disc Disease ◽  
Reactive Protein ◽  
Postoperative Serum ◽  
Wbc Count ◽  
And Control

Purpose: C-reactive protein (CRP) and white blood cell (WBC) count are routine blood chemistry parameters in monitoring infection. Little is known about the natural history of their serum levels in conservative and operative spondylodiscitis treatment. Methods: Pre- and postoperative serum levels of CRP and WBC count in 145 patients with spondylodiscitis were retrospectively assessed. One hundred and four patients were treated by debridement, spondylodesis, and an antibiotic regime, 41 only with a brace and antibiotics. The results of the surgical group were compared to 156 patients fused for degenerative disc disease (DDD). Results: Surgery had a significant effect on peak postoperative CRP levels. In surgically managed patients, CRP peaked at 2–3 days after surgery (spondylodiscitis: pre-OP: 90 mg/dl vs. post-OP days 2–3: 146 mg/dl; DDD: 9 mg/dl vs. 141 mg/dl; p < 0.001), followed by a sharp decline. Although values were higher for spondylodiscitis patients, dynamics of CRP values were similar in both groups. Nonoperative treatment showed a slower decline. Surgically managed spondylodiscitis showed a higher success rate in identifying bacteria. Specific antibiotic treatment led to a more predictable decline of CRP values. WBC did not show an interpretable profile. Conclusion: CRP is a predictable serum parameter in patients with spondylodiscitis. WBC count is unspecific. Initial CRP increase after surgery is of little value in monitoring infection. A preoperative CRP value, and control once during the first 3 days after surgery is sufficient. Closer monitoring should then be continued. Should a decline not be observed, therapy needs to be scrutinized, antibiotic treatment reassessed, and concomitant infection contemplated.

scholarly journals Infection Prevention Considerations for a Multi-Mission Convention Center Field Hospital in Baltimore, Maryland during the COVID-19 Pandemic

2021 ◽  
pp. 1-21
Author(s):  
Jennifer A. Jones ◽  
Zishan K. Siddiqui ◽  
Charles Callahan ◽  
Surbhi Leekha ◽  
Sharon Smyth ◽  
...  
Keyword(s):  
Best Practices ◽  
Infection Prevention ◽  
Diagnostic Testing ◽  
The United States ◽  
Infection Prevention And Control ◽  
Field Hospital ◽  
First Case ◽  
Convention Center ◽  
Set Up ◽  
And Control

Abstract The state of Maryland identified its first case of COVID-19 on March 5, 2020. The Baltimore Convention Center (BCCFH) quickly became a selected location to set up a 250-bed inpatient Field Hospital and Alternate Care Site. In contrast to other field hospitals throughout the United States, the BCCFH remained open throughout the pandemic and took on additional COVID-19 missions, including community SARS-CoV-2 diagnostic testing, monoclonal antibody infusions for COVID-19 outpatients, and community COVID-19 vaccinations. At the time of publication, the BCCFH had cared for 1,478 COVID-19 inpatients, performed 108,155 COVID-19 tests, infused 2,166 COVID-19 patients, and administered 115,169 doses of COVID-19 vaccine. To prevent the spread of pathogens during operations, infection prevention and control guidelines were essential to ensure the safety of staff and patients. Through multi-agency collaboration, utilization of infection prevention best practices, and answering what we describe as “PPE-ESP”, an operational framework was established to reduce infection risks for those providing or receiving care at the BCCFH during the COVID-19 pandemic.

scholarly journals Real time surveillance of COVID-19 space and time clusters during the summer 2020 in Spain

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicolás Rosillo ◽  
Javier Del-Águila-Mejía ◽  
Ayelén Rojas-Benedicto ◽  
María Guerrero-Vadillo ◽  
Marina Peñuelas ◽  
...  
Keyword(s):  
Real Time ◽  
Scan Statistic ◽  
Cluster Aggregation ◽  
Space And Time ◽  
Spatial Trends ◽  
Community Transmission ◽  
Low Incidence ◽  
Set Up ◽  
And Control ◽  
Time Scan

Abstract Background On June 21st de-escalation measures and state-of-alarm ended in Spain after the COVID-19 first wave. New surveillance and control strategy was set up to detect emerging outbreaks. Aim To detect and describe the evolution of COVID-19 clusters and cases during the 2020 summer in Spain. Methods A near-real time surveillance system to detect active clusters of COVID-19 was developed based on Kulldorf’s prospective space-time scan statistic (STSS) to detect daily emerging active clusters. Results Analyses were performed daily during the summer 2020 (June 21st – August 31st) in Spain, showing an increase of active clusters and municipalities affected. Spread happened in the study period from a few, low-cases, regional-located clusters in June to a nationwide distribution of bigger clusters encompassing a higher average number of municipalities and total cases by end-August. Conclusion STSS-based surveillance of COVID-19 can be of utility in a low-incidence scenario to help tackle emerging outbreaks that could potentially drive a widespread transmission. If that happens, spatial trends and disease distribution can be followed with this method. Finally, cluster aggregation in space and time, as observed in our results, could suggest the occurrence of community transmission.

scholarly journals Development of an Exercise Training Protocol to Investigate Arteriogenesis in a Murine Model of Peripheral Artery Disease

2019 ◽  
Vol 20 (16) ◽  
pp. 3956 ◽  
Author(s):  
Ayko Bresler ◽  
Johanna Vogel ◽  
Daniel Niederer ◽  
Daphne Gray ◽  
Thomas Schmitz-Rixen ◽  
...  
Keyword(s):  
Peripheral Artery Disease ◽  
Suitable Model ◽  
Peripheral Artery ◽  
Running Wheel ◽  
Limb Perfusion ◽  
Collateral Growth ◽  
Artery Disease ◽  
Set Up ◽  
Exercise Induced ◽  
And Control

Exercise is a treatment option in peripheral artery disease (PAD) patients to improve their clinical trajectory, at least in part induced by collateral growth. The ligation of the femoral artery (FAL) in mice is an established model to induce arteriogenesis. We intended to develop an animal model to stimulate collateral growth in mice through exercise. The training intensity assessment consisted of comparing two different training regimens in C57BL/6 mice, a treadmill implementing forced exercise and a free-to-access voluntary running wheel. The mice in the latter group covered a much greater distance than the former pre- and postoperatively. C57BL/6 mice and hypercholesterolemic ApoE-deficient (ApoE−/−) mice were subjected to FAL and had either access to a running wheel or were kept in motion-restricting cages (control) and hind limb perfusion was measured pre- and postoperatively at various times. Perfusion recovery in C57BL/6 mice was similar between the groups. In contrast, ApoE−/− mice showed significant differences between training and control 7 d postoperatively with a significant increase in pericollateral macrophages while the collateral diameter did not differ between training and control groups 21 d after surgery. ApoE−/− mice with running wheel training is a suitable model to simulate exercise induced collateral growth in PAD. This experimental set-up may provide a model for investigating molecular training effects.

scholarly journals How to take advantage of tablet computers: Effects of news structure on recall and comprehension

2015 ◽  
Vol 40 (4) ◽  
Author(s):  
Anna Van Cauwenberge ◽  
Leen d’Haenens ◽  
Hans Beentjes
Keyword(s):  
Eye Tracking ◽  
Tablet Computers ◽  
Tracking Method ◽  
News Stories ◽  
News Consumption ◽  
Set Up ◽  
And Control ◽  
Factual Recall

AbstractIn light of the growing use of tablets for news reading and mobile news consumption behaviors, this study examined whether an innovative way of structuring news on the tablet that mimics mobile news behaviors reinforced attention for, and learning from, news. Specifically, it was theorized that the chronological and associative structuring of news articles into so-called developing news stories would lead to more attention for news, and better recall and comprehension of news, than the linear print newspaper structure that newspaper publishers continue to copy from print to tablet. A multiple-day experiment was set up using the eye-tracking method to measure and control for attention. The results show that the developing news structure increased comprehension of news substantively, independently of attention effects; no effects were found on attention and factual recall.

NEURAL MODELING AND CONTROL OF DYNAMIC SYSTEMS WITH HYSTERESIS

2007 ◽  
Vol 31 (1) ◽  
pp. 127-141
Author(s):  
Yonghong Tan ◽  
Xinlong Zhao
Keyword(s):  
Dynamic Systems ◽  
Control Strategy ◽  
Neural Modeling ◽  
Modeling And Control ◽  
Control Scheme ◽  
The Neural Networks ◽  
Adaptive Control Strategy ◽  
Set Up ◽  
Modeling Strategy ◽  
And Control

A hysteretic operator is proposed to set up an expanded input space so as to transform the multi-valued mapping of hysteresis to a one-to-one mapping so that the neural networks can be applied to model of the behavior of hysteresis. Based on the proposed neural modeling strategy for hysteresis, a pseudo control scheme is developed to handle the control of nonlinear dynamic systems with hysteresis. A neural estimator is constructed to predict the system residual so that it avoids constructing the inverse model of hysteresis. Thus, the control strategy can be used for the case where the output of hysteresis is unmeasurable directly. Then, the corresponding adaptive control strategy is presented. The application of the novel modeling approach to hysteresis in a piezoelectric actuator is illustrated. Then a numerical example of using the proposed control strategy for a nonlinear system with hysteresis is presented.

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