scholarly journals Protective Effect of the Mixture of Stem bark, Leaf and Root Extract of Bauhinia acuminata L. against Carbon tetrachloride induced Liver Damage in Wistar Albino Rats

2021 ◽  
Vol 01 (05) ◽  
Author(s):  
Venkatesh Sellamuthu ◽  
Keyword(s):  
Carbon Tetrachloride ◽  
Vitamin C ◽  
Stem Bark ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Standard Result ◽  
Soxhlet Apparatus ◽  
Study Results

Objective: The objective of the present study was to evaluate the hepatoprotective effect of mixture of stem bark, leaf and root extract of Bauhinia acuminata L. using carbon tetrachloride induced hepatotoxicity model. Method: Powdered mixture materials were extracted with distilled water using soxhlet apparatus. The dried extracts were subjected to preliminary phytochemical analysis and the extracts were evaluated for acute oral toxicity by OECD guidelines 423.The aqueous extract at a dose level 200 mg/kg and 400 mg/kg were selected and administered to albino rats for hepatoprotective screening. Hepatotoxicity was induced by Carbon tetrachloride (1 ml/kg with CMC). Silymarin (100 mg/kg p. o) was used as the standard. Result: The preliminary phytochemical examination showed the presence of Alkaloids, Glycosides, Saponins, Flavanoids, Tannins, Aminoacids, Tryptophan, Quinones, Terpinoids, Starch, Vitamin C and Carbohydrates. Furthermore, the acute toxicity study results showed that the extracts were found to be safe up to 2000 mg/kg b. wt. The extract dose dependently shows hepatoprotective potential by restoring the elevated biochemical parameters. Conclusion: The results obtained from the study indicates that mixture of stem bark, leaves and root extract of Bauhinia acuminata L. possess promising hepatoprotective activity. The activity might be due the presence of the phytoconstituents including Alkaloids, Glycosides, Saponins, Flavanoids, Tannins, Aminoacids, Carbohydrates Terpinoids, Starch, Vitamin C, Tryptophan and Quinones in the extract. Further studies are required to identify the active principle responsible for the hepatoprotective activity.

scholarly journals Antihepatotoxic Activity of Phyllanthus atropurpureus Cultivated in Egypt

2011 ◽  
Vol 66 (9-10) ◽  
pp. 447-452 ◽  
Author(s):  
Taha Sarg ◽  
Afaf Abdel Ghani ◽  
Rawia Zayed ◽  
May El-Sayed
Keyword(s):  
Liver Injury ◽  
Active Ingredient ◽  
Ornamental Plants ◽  
Hepatoprotective Activity ◽  
Maximum Activity ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Aerial Parts ◽  
Serum Aspartate Aminotransferase ◽  
Antihepatotoxic Activity

The genus Phyllanthus (family Euphorbiaceae) is considered one of the important medicinal and ornamental plants. A phytochemical analysis of the extracts was performed to search for the active ingredient. Results of the investigation of the hepatoprotective activity of Phyllanthus atropurpureus Boj. Hort. Maurit. revealed that the activities of alcoholic extracts of its aerial parts and roots were quite similar to those of silymarin. Both of them improve the parameters of CCl4-induced liver injury including serum aspartate aminotransferase and alanine aminotransferase. Among the extracts tested, the root extract showed maximum activity compared to the aerial parts extract and to silymarin.

COMPARATIVE EVALUATION OF IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITIES OF ETHANOLIC LEAF AND ROOT EXTRACTS OF CLEOME GYNANDRA

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 50-56
Author(s):  
K Ravishankar ◽  
Y.V.V.M. Lakshmi Prasanna ◽  
G.V.N. Kiranmayi ◽  
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatoprotective Activity ◽  
Root Extract ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Root Extracts ◽  
Cleome Gynandra ◽  
Ic50 Values ◽  
Ethanolic Leaf Extract

In vitro antioxidant and in vivo hepatoprotective activities of Cleome gynandra ethanolic leaf and root extracts were assessed. In vitro antioxidant activity was carried by DPPH, Nitric oxide, hydroxyl radical and phosphomolybdenum assays. Hepatoprotective activity was evaluated by Carbon tetrachloride (CCl4) induced hepatotoxicity in albino rats.The animals were divided into seven groups (Four test groups - Ethanolic Leaf and Root Extracts of Cleome gynandra of 100 mg/kg and 200 mg/kg, standard silymarin (100 mg/kg), toxic control-carbon tetrachloride and vehicle). On the eight day, the blood was collected and parameters like serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), Alkaline phosphatase (ALP) and Total bilirubin (TB) were estimated. Significant antioxidant status with good IC50 values similar to standard ascorbic acid was obtained. A significant decrease in liver enzymes was observed in test groups comparable to silymarin. From the results obtained, ethanolic leaf extract has contributed better hepatoprotection compared with root extract in experimental rats.

scholarly journals WOUND HEALING POTENTIAL OF METHANOLIC EXTRACT OF FLOWERS OF BUTEA MONOSPERMA LINN. IN DIABETIC ANIMALS

2018 ◽  
Vol 8 (5-s) ◽  
pp. 306-310
Author(s):  
Shweta Panwar ◽  
Neetesh Kumar Jain ◽  
MK Gupta
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Methanolic Extract ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Butea Monosperma ◽  
Glucose Levels ◽  
Wound Model ◽  
Soxhlet Apparatus ◽  
Alloxan Monohydrate

The main of our study is to evaluate the Wound Healing Potential of Methanolic Extract of Flowers of Butea monosperma Linn. in Diabetic Animals. Methanolic extract was prepared by continuous hot extraction method by soxhlet apparatus. Preliminary phytochemical screening showed the presence of flavonoids, phenolic compounds and some glycosides. Diabetes was induced by single injection of Alloxan monohydrate in Wistar albino rats and their blood glucose levels were measured. Excision wound model was used for creation of wound in diabetic animals and methanolic extract was administered in diabetic animals to observe its effect. Methanolic extract significantly lowered the blood glucose level and highly significantly showed wound contraction in diabetic animals. The preliminary phytochemical analysis of the Butea monosperma flower extract showed the presence of tannins, flavonoids and triterpenoids. As per previous literature survey, flavonoids are responsible for anti-diabetic and wound healing activity. So this wound healing effect of Butea monosperma may be due to presence of flavonoids in plant. Hence present research supports traditional claims of the plant in wound healing. Keywords: Butea monosperma, Diabetic animals, Alloxan monohydrate, Methanolic Extract, Wound Area

scholarly journals ANTIOXIDANT PROPERTY OF ANTIHYPERTENSIVE DRUGS (CARVEDILOL, ENALAPRIL, AND AMLODIPINE) ON LIVER FUNCTION IN ANIMAL (RATS) MODELS

2016 ◽  
pp. 292
Author(s):  
Pusuloori Rajesh*
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Markers ◽  
Antihypertensive Drugs ◽  
Beta Blockers ◽  
Serum Levels ◽  
Antioxidant Property ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamate Pyruvate Transaminase

<p>ABSTRACT<br />Objective: The antihypertensive drugs (beta-blockers - carvedilol, calcium channel blocker - amlodipine, and angiotensin-converting enzyme<br />inhibitor - enalapril) were investigated for its hepatoprotective effect by antioxidant property against carbon tetrachloride (CCl<br />)-induced acute liver<br />damage on albino rats. <br />Methods: In this study the liver was damaged by giving Carbon tetra chloride (CCl<br />4<br />)-1 ml/kg-p.o. After giving CCl<br /> (1 ml/kg), it significantly elevated<br />the serum levels of biochemical markers such as serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline<br />phosphatase, total bilirubin, protein, and depleted antioxidant enzymes glutathione. After treatment with carvedilol (10 mg/kg), enalapril (0.5 mg/kg),<br />and amlodipine (10 mg/kg) for 2 weeks, these drugs were significantly reduces the elevated levels of biochemical markers mentioned above.<br />Conclusion: These results suggest that these antihypertensive drugs may have the potential therapeutic value in the treatment of CCl<br />4<br />-induced<br />hepatic damage and some liver diseases. Hepatoprotective activity of these drugs may be attributed to the antioxidant principles in it.<br />Keywords: Antioxidant, Carvedilol, Enalapril, Amlodipine, Carbon tetrachloride, Hepatoprotective.<br />4</p><p>4</p>

scholarly journals Hepatoprotective effects of Tagetes lucida root extract in carbon tetrachloride-induced hepatotoxicity in Wistar albino rats through amelioration of oxidative stress

2021 ◽  
Vol 59 (1) ◽  
pp. 986-997
Author(s):  
Samah Ali El-Newary ◽  
Rasha Fouad Ismail ◽  
Nermeen Mohammed Shaffie ◽  
Saber Fayez Hendawy ◽  
Elsayed Omer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Albino Rats ◽  
Root Extract ◽  
Hepatoprotective Effects ◽  
Wistar Albino Rats

scholarly journals PHYTOCHEMICAL EXTRACTIONS, QUALITATIVE ANALYSIS, AND EVALUATION OF ANTIMICROBIAL ACTIVITY IN THE LEAF AND STEM BARK OF SOLANUM PUBESCENS WILLD.

2019 ◽  
pp. 280-284
Author(s):  
SUDHAMA VN ◽  
RAMAKRISHNAN M
Keyword(s):  
Antimicrobial Activity ◽  
Qualitative Analysis ◽  
Fungal Pathogens ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Soxhlet Apparatus ◽  
Solanum Pubescens

Objective: The objective of this study was to extract the phytochemicals from leaf and stem bark of Solanum pubescens, perform qualitative analysis, and evaluate antimicrobial activity of the phytochemicals against certain microbial pathogens. Methods: Leaf and stem bark of S. pubescens was subjected to phytochemical extractions using Soxhlet apparatus with five different solvents, identified the major constituents in different solvent extracts using standard protocols and investigated the potential antimicrobial activities of the extracts against certain selected bacterial and fungal pathogens by agar well diffusion method. Results: The maximum yield was in methanolic extracts of leaf and stem bark of the study plant such as 18.51 and 12.5%, respectively, followed by the hydroalcohol extracts (14.23 and 10.00%). Qualitative phytochemical analysis revealed maximum number of extracted bioactive compounds when compared to other similar studies. Among the five different solvent extracts of S. pubescens, n-hexane extract of stem bark was found to be active against all the bacterial pathogens and stem bark extracts made with methanol and hydroalcohol showed antifungal activity against all the four fungal pathogens tested. Leaf extracts made with solvents such as n-hexane and ethyl acetate showed inhibition against Klebsiella pneumoniae and Staphylococcus aureus, respectively, hydroalcohol extract was active against Pseudomonas aeruginosa and Salmonella typhimurium while none of the leaf extracts of the study plant showed inhibition zones against fungal pathogens tested. Conclusion: Multiple solvent extraction approach yielded the phytochemicals which are not yet reported and the antimicrobial activities of phytochemicals acknowledged their medicinal value.

scholarly journals Curative Effects of Aqueous and Ethanol Stem Bark Extracts of Vitex doniana on Doxorubicin-Induced Cardiotoxicity in Rats

2021 ◽  
pp. 8-17
Author(s):  
Mohammed A. Sulaiman ◽  
Daniel Dahiru ◽  
Mahmoud S. Jada ◽  
Ahmed I. Hayatu
Keyword(s):  
Oral Treatment ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Traditional Healers ◽  
Serum Marker ◽  
Serum Markers ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Vitex Doniana

Background: Cardiovascular diseases (CVDs) constitute the number one cause of mortality at the global level, representing 30% of all global deaths. Therefore, finding ways to reduce deaths due to CVDs remain an important public health goal. Traditional healers in northern Nigeria use the stem bark of Vitex doniana to treat hypertensive patients. This study was aimed to investigate the cardiocurative potential of Vitex doniana on doxorubicin-induced Cardiotoxicity in rats. Methods: Thirty five (35) adult Albino rats weighing 175 ± 25 g were used, of which 30 were induced with cardiotoxicity by intraperitoneal injection of doxorubicin (10 mg/kg) for three consecutive days. Rats were treated by oral administration of Silymarin (100 mg/kg) and Vitex doniana aqueous or ethanol extract (100 mg/kg and 200 mg/kg) for 14 consecutive days and thereafter were sacrificed on the 15th day. Blood, plasma and serum were analyzed for lipid profile and serum markers for cardiotoxicity. Results: Phytochemical analysis of the extracts showed the presence of alkaloids, tannins, flavonoids, steroids, phenols, saponins, terpenoids and glycosides. Oral treatment with Vitex doniana extracts significantly (p<0.05) lowered the elevated levels of total cholesterol, triglycerides and LDL but significantly (p<0.05) increased the level of HDL (18.61 ± 0.55 mg/dl to 57.98 ± 0.78 mg/dl). The extracts also significantly (p<0.05) decreased the levels of serum marker enzymes for cardiotoxicity ALT, AST, CK – mb and LDH. Conclusion: The prophylactic cardiocurative use of Vitex doniana stem bark has been confirmed in this study as the extracts exhibited hypolipidemic and cardiocurative effects in dose dependent manner in doxorubicin-induced cardiotoxicity rat model.

scholarly journals Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains

2010 ◽  
Vol 4 (05) ◽  
pp. 292-300 ◽  
Author(s):  
Rosina Khan ◽  
Mohammad Zakir ◽  
Sadul H Afaq ◽  
Abdul Latif ◽  
Asad U Khan
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Zingiber Officinale ◽  
Multidrug Resistant ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Trachyspermum Ammi ◽  
Soxhlet Apparatus ◽  
Study Results

Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes. Methodology: Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed .Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study. Results: The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 µg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 µg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 µg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed. Conclusions: The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents.

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