Evaluation of hepatoprotective activity of the ethanolic extract of leaves of Mimosa pudica linn in carbon tetrachloride induced hepatic injury in albino rats

2010 ◽

Vol 38 (01) ◽

pp. 75-88 ◽

Cited By ~ 18

Author(s):

Saleh Alqasoumi

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Liver Tissue ◽

Hepatic Injury ◽

Liver Toxicity ◽

Elevated Serum ◽

Ethanolic Extract ◽

Albino Rats ◽

Eruca Sativa ◽

Protein Sulfhydryl

The hepatoprotective and antioxidant effect of an ethanolic extract of 'Rocket' Eruca sativa L. (EER), on liver injury induced by carbon tetrachloride ( CCl4 ) was investigated. Wistar albino rats were administered 250 and 500 mg/kg body weight extract orally for 10 consecutive days. Marker enzymes GOT, GPT, ALP, GGT and bilirubin were estimated in serum. Whereas, non-protein sulfhydryl (NP-SH), total protein (TP) and malondialdehyde (MDA) were estimated in liver tissue as markers for oxidative stress. Histopathological assessment was also done on liver tissue. CCl4 induced liver poisoning in all treated animals was evident by elevated serum GOT, GPT, ALP, GGT and bilirubin levels. Induction of oxidative stress in the liver tissue by CCl4 was evidenced by a fall in the levels of NP-SH and TP; and an increased level of MDA concentration. EER administration for 10 days prevented the CCl4 induced hepatic injury and oxidative stress. Furthermore, the extract also reduced the pentobarbital-induced prolongation of sleeping time in mice. The ability of rocket extract to protect the liver toxicity in rats was further confirmed by histological findings in the liver tissue. In conclusion, it was observed that Eruca sativa L. extract protects the liver against CCl4 induced hepatic injury through its potent antioxidant activity in rats.

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HEPATOPROTECTIVE EFFECTS OF POLYHERBAL FORMULATION AGAINST CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY IN ALBINO RATS: A TOXICITY SCREENING APPROACH

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i1.14757 ◽

2016 ◽

Vol 10 (1) ◽

pp. 192 ◽

Cited By ~ 1

Author(s):

Bhumika Yogi ◽

Ashutosh Mishra

Keyword(s):

Carbon Tetrachloride ◽

Hepatic Injury ◽

Methanolic Extract ◽

Hepatoprotective Activity ◽

Calotropis Procera ◽

Albino Rats ◽

Gymnema Sylvestre ◽

Toxicity Screening ◽

Lawsonia Inermis ◽

Polyherbal Formulation

ABSTRACTObjective: To investigate the hepatoprotective activity of aqueous methanolic extract of polyherbal formulation and individual extracts of Calotropisprocera, Gymnema sylvestre, and Lawsonia inermis leaves.Methods: The hepatoprotective activity of the formulation and extracts were assessed on the basis of histopathological changes in liver of carbontetrachloride-induced hepatotoxic rats.Results: The result of present studies strongly indicates that the hepatoprotective property of polyherbal formulation as evidenced by less damagedhepatocytes cells.Conclusion: Polyherbal formulation revealed excellent hepatoprotective profile as compared to individual plants due to synergistic effect producedby different phytochemicals present in the formulation.Keywords: Calotropis procera, Gymnema sylvestre, Lawsonia inermis, Carbon tetrachloride.

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Assessment of hepatoprotective activity of coded plant (222) leaf ethanolic extract against carbon tetrachloride-induced hepatotoxicity in Wistar rats – Part IV

Journal of Traditional and Folk Practices ◽

10.25173/jtfp.2017.5.2.78 ◽

2018 ◽

Vol 5 (2) ◽

Author(s):

Krishnakumar N M

Keyword(s):

Carbon Tetrachloride ◽

Biochemical Parameters ◽

Subcutaneous Administration ◽

Ethanolic Extract ◽

Treated Group ◽

Hepatoprotective Activity ◽

Control Group ◽

Albino Rats ◽

Hepatic Enzymes ◽

Standard Drug

The present study was designed to assess the possible hepatoprotective activity of the leaf ethanolic extract of coded plant (Code No. 222**) against carbon tetrachloride (CCl4)-induced hepatic injury in Wistar albino rats. The animals were divided into different groups and treated with 222 leaf ethanolic extract at different concentrations for five days. Silymarin, the known hepatoprotective standard compound (100 mg/kg) was administered for five days. Hepatotoxicity was induced by the subcutaneous administration of a single dose of CCl4: Olive oil (2 mL/kg) on days 2 and 3. The administration of CCl4 resulted in marked increase in serum hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum bilirubin levels. CCl4 intoxication also resulted in a significant (P=0.05) increase in malondialdehyde (MDA), which is a common marker of lipid peroxidation. The other biochemical parameters such as cholesterol, triglycerides, creatinine, urea and uric acid levels were also increased significantly (P=0.05) compared to normal control group. Changes in serum hepatic enzymes, biochemical parameters and MDA levels induced by CCl4 were reversed by the leaf ethanolic extract of 222 (125 mg/kg) dose. The standard drug silymarin treated group also reversed CCl4-induced changes in biomarkers of liver function and MDA levels. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was seen that histopathological damage induced by CCl4 were improved in rat liver, treated with 222 extract. The results of the present study suggested that coded plant (222) leaf ethanolic extract may be used as a hepatoprotective agent against toxic effects caused carbon tetrachloride in the liver.

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Role of Emblica officinalis (amla) in the prophylaxis of hepatic injury by carbon tetrachloride (CCl4) in albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20173284 ◽

2017 ◽

Vol 6 (8) ◽

pp. 1992

Author(s):

Chinmoyee Deori ◽

Swarnamoni Das ◽

Sushanta Kumar Bordoloi

Keyword(s):

Carbon Tetrachloride ◽

Hepatic Injury ◽

Cost Effective ◽

Hepatoprotective Activity ◽

Modern Medicine ◽

Albino Rats ◽

Emblica Officinalis ◽

Oral Toxicity ◽

Standard Drug ◽

Percolation Method

Background: The objective of the study was to evaluate the hepatoprotective activity of fruits extract of Emblica officinalis on carbon tetrachloride induced hepatotoxicity in Albino rats. Liver is vulnerable to wide variety of metabolic, toxic, microbial, circulatory and neoplastic insults. The dominant primary diseases of the liver are viral hepatitis, alcoholic liver disease and hepatocellular carcinoma. Due to absence of reliable drugs management of liver ailments in the modern medicine, plants and natural products are providing to be good hepatoprotectants as evident from voluminous published work on their hepatoprotective potentials.Methods: The required amount of fresh fruits of Emblica officinalis was collected and dried at room temperature. The dried fruit ground into powdered and was prepared by percolation method using 95% ethanol. Acute oral toxicity test of the fruits extract was performed as per OECD 423 guidelines. Carbon tetrachloride was used as the hepatotoxin. Silymarin suspension 100mg was used as a standard drug in this study.Results: The study was carried out with an attempt to evaluate the hepatoprotective activity of Emblica officinalis in carbon tetrachloride induced hepatic injury in albino rats. The results were analysed by using one way ANOVA followed by Dunnett’s test and (p <0.05) was considered significant.Conclusions: The present study shows that the Emblica officinalis has significant hepatoprotective activity in rats with carbon tetrachloride induced liver injury. Emblica officinalis is a commonly used traditional herb, which is safe, cost effective and grows widely in India and used by different ethnic groups for various diseases. That is why Emblica officinalis, a commonly used natural herb, deserve further consideration in order to establish its potential as safe economical hepatoprotective agent.

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Ameliorative effect of Ethanol Extract of Annona Muricata Leaves in Sodium Arsenite Induced- Toxicity in Male Wistar Rats

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2021/v30i230249 ◽

2021 ◽

pp. 8-15

Author(s):

Adegboyega A. M. ◽

Odunola O. A. ◽

Akinwumi K. A. ◽

Babalola O. ◽

Akinwande A.

Keyword(s):

Body Weight ◽

Sodium Arsenite ◽

Hepatic Injury ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Annona Muricata ◽

Ameliorative Effect ◽

Male Wistar Rats

Ingestion of arsenic, a known contaminant in drinking water causes cancer at multiple tissues and there is no cure. Consumption of arsenic contaminated water has been implicated metalloid-induced carcinogenesis. Research is therefore directed at chemoprevention using medicinal herbs for the management of arsenicosis. In this study hepatoprotective activity of ethanolic extract of Annona muricata (AM) leaves was assessed against sodium arsenite (SA) induced hepatic injury in albino rats. The animals were pre-treated with either 250 or 500mg/kg body weight of rat before exposure to SA. SA was dissolved in distilled water and administered at a dose of 5 mg/kg body weight on the 7th, 14th and 21st day of the experiment. SA was observed to induce a significant increase (p < 0.05) in serum aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase activities (ALP). However, pretreatments of rats with various doses of AM significantly (P<0.005) reduced serum enzyme levels to near normal against SA treated rats. Furthermore, histopathological observations revealed that treatment with AM extract protected the animals from SA induced liver damage. The results indicated that the leaves of Annona muricata possess hepatoprotective activity on SA induced hepatic injury in rats.

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Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

BioMed Research International ◽

10.1155/2014/240243 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Kannappan Poornima ◽

Palanisamy Chella Perumal ◽

Velliyur Kanniappan Gopalakrishnan

Keyword(s):

Ethanolic Extract ◽

Treated Group ◽

Hepatic Necrosis ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Protective Effects ◽

Liver Necrosis ◽

Standard Drug ◽

Tabernaemontana Divaricata ◽

Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

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