scholarly journals Bacterial Strain Isolated from High-Salt Environments Can Produce Large Amounts of New Polyhydroxyalkanoate (PHA)

2020 ◽  
Vol 8 (4) ◽  
pp. 1268-1273
Keyword(s):  
Screening Program ◽  
Salt Lake ◽  
Bacterial Species ◽  
Extreme Environment ◽  
Rrna Gene ◽  
Fars Province ◽  
Bacillus Endophyticus ◽  
High Production ◽  
Pha Production ◽  
The 16S Rrna Gene

Since the major problem connected to the industrial production of Polyhydroxyalkanoates (PHAs) is their high production price, this study was performed to inspect the new potential bacterial species for industrial PHA production. The bacterial samples were collected during a screening program from Pink Salt Lake as an extreme environment in the south of Iran, Fars province. The PHA-producing bacteria were isolated. Then further studies on different morphological, cultural, and physiological characteristics of isolates were performed. Among the isolated microorganisms in this study, 18 of 143 bacteria were selected as PHA-producer microorganisms to be studied for analysis along with a partial sequence of the 16S rRNA gene. This study introduces two bacteria; Bacillus endophyticus BCCS 011 and Lysobacter sp. BCCS 052 as new potential PHA producer that has not been reported previously. They could be an ideal option for cheaper PHAs production.

scholarly journals Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources

Biomolecules ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 109 ◽  
Author(s):  
Manel Ben Abdallah ◽  
Fatma Karray ◽  
Sami Sayadi
Keyword(s):  
Gas Chromatography ◽  
Salt Lake ◽  
Maximum Yield ◽  
Carbon Sources ◽  
Extreme Environment ◽  
Industrial Wastes ◽  
High Yield ◽  
Rrna Gene ◽  
Gravimetric Analysis ◽  
Pha Production

The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to Natrinema altunense and Haloterrigena jeotgali, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield.

Differentiation of Staphylococcus spp. by high-resolution melting analysis

2010 ◽  
Vol 56 (12) ◽  
pp. 1040-1049 ◽  
Author(s):  
Michal Slany ◽  
Martina Vanerkova ◽  
Eva Nemcova ◽  
Barbora Zaloudikova ◽  
Filip Ruzicka ◽  
...  
Keyword(s):  
High Resolution ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
High Resolution Melting ◽  
Bacterial Species ◽  
High Resolution Melting Analysis ◽  
Rrna Gene ◽  
Staphylococcus Capitis ◽  
Melting Analysis ◽  
The 16S Rrna Gene

High-resolution melting analysis (HRMA) is a fast (post-PCR) high-throughput method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range PCR within the 16S rRNA gene where sequence differences are minimal. Genomic DNA samples isolated from 12 reference staphylococcal strains ( Staphylococcus aureus , Staphylococcus capitis , Staphylococcus caprae , Staphylococcus epidermidis , Staphylococcus haemolyticus , Staphylococcus hominis , Staphylococcus intermedius , Staphylococcus saprophyticus , Staphylococcus sciuri , Staphylococcus simulans , Staphylococcus warneri , and Staphylococcus xylosus ) were subjected to a real-time PCR amplification of the 16S rRNA gene in the presence of fluorescent dye EvaGreen™, followed by HRMA. Melting profiles were used as molecular fingerprints for bacterial species differentiation. HRMA of S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unrelated strain.

scholarly journals Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Maki Teramoto ◽  
Zhenyu Zhai ◽  
Ayumi Komatsu ◽  
Keigo Shibayama ◽  
Masato Suzuki
Keyword(s):  
Genome Sequence ◽  
Gene Sequence ◽  
Virulence Genes ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Fish Pathogen ◽  
Deep Seawater ◽  
Fish Pathogens ◽  
The 16S Rrna Gene

Some bacterial species of the genus Tenacibaculum , including Tenacibaculum ovolyticum , have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for Tenacibaculum dicentrarchi , which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of T. ovolyticum DSM 18103 T , was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus Tenacibaculum .

Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 3975-3980 ◽  
Author(s):  
Xing-Xing Qiu ◽  
Yun-Zhuang Mou ◽  
Mei-Lin Zhao ◽  
Wen-Jiao Zhang ◽  
Dong Han ◽  
...  
Keyword(s):  
Salt Lake ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Content Type ◽  
Optimum Growth Temperature ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

Two halophilic archaeal strains, YC20T and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l−1 and 0.5–95 g MgCl2 l−1 for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0–95.9 % similarity to the most closely related members of the genus Halobellus of the family Halobacteriaceae . The rpoB′ gene sequence similarity between strains YC20T and XD15 was 98.2 % and these sequences showed 89.6–92.8 % similarity to those of the most closely related members of the genus Halobellus . The DNA G+C contents of strains YC20T and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA–DNA hybridization value between strain YC20T and strain XD15 was 92 %, and the two strains showed low DNA–DNA relatedness to members of the genus Halobellus . The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20T and XD15 represent a novel species of the genus Halobellus , for which the name Halobellus inordinatus sp. nov. is proposed. The type strain is YC20T ( = CGMCC 1.12120T = JCM 18361T) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).

scholarly journals Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 632-637 ◽  
Author(s):  
Song-Ih Han ◽  
Hyo-Jin Lee ◽  
Hae-Ran Lee ◽  
Ki-Kwang Kim ◽  
Kyung-Sook Whang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Bacterial Species ◽  
Angelica Sinensis ◽  
Rrna Gene ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

Three exopolysaccharide-producing bacteria, designated strains DRP28T, DRP29 and DRP31, were isolated from the rhizoplane of Angelica sinensis from the Geumsan, Republic of Korea. Cells were straight rods, Gram reaction-negative, aerobic, non-motile, and catalase- and oxidase- positive. Flexirubin-type pigments were absent. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Mucilaginibacter in the phylum Bacteroidetes. 16S rRNA gene sequence similarities to strains of recognized species of the genus Mucilaginibacter were 93.8–97.4 %. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The strains contained MK-7 as the major isoprenoid quinone. Strains DRP28T, DRP29 and DRP31 formed a single, distinct genomospecies with DNA G+C contents of 41.9–42.7 mol% and DNA hybridization values of 82.6–86.8 %; the strains exhibited DNA–DNA hybridization values of only 20.4–41.3 % with related species of the genus Mucilaginibacter. On the basis of evidence presented in this study, strains DRP28T, DRP29 and DRP31 were considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter polysacchareus sp. nov. is proposed. The type strain is DRP28T ( = KACC 15075T  = NBRC 107757T).

Haloterrigena saccharevitans sp. nov., an extremely halophilic archaeon from Xin-Jiang, China

2005 ◽  
Vol 55 (6) ◽  
pp. 2539-2542 ◽  
Author(s):  
Xue-Wei Xu ◽  
Shuang-Jiang Liu ◽  
Dilbar Tohty ◽  
Aharon Oren ◽  
Min Wu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Extremely Halophilic Archaeon ◽  
Taxonomic Characterization ◽  
Derivatives Of ◽  
The 16S Rrna Gene ◽  
Haloterrigena Turkmenica

A novel extremely halophilic strain, isolated from Aibi salt lake, Xin-Jiang, China, was subjected to polyphasic taxonomic characterization. This strain, designated AB14T, is neutrophilic, motile and requires at least 10 % (w/v) NaCl for growth. Strain AB14T grows at 24–58 °C, with optimal growth at 42–45 °C. Mg2+ is not required, but growth is observed in MgCl2 concentrations as high as 1·0 M. Strain AB14T possesses the diphytanyl (C20C20) and phytanyl-sesterterpanyl diether (C20C25) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6 disulfate 1→2 glucose-glycerol diether. The genomic DNA G+C content is 66·6 mol%. The 16S rRNA gene sequence similarity values of strain AB14T with its nearest phylogenetic neighbours (Haloterrigena thermotolerans and Haloterrigena turkmenica) are 98·6 and 96·0 %, respectively. DNA–DNA hybridization revealed 54 % relatedness between strain AB14T and Haloterrigena thermotolerans JCM 11050T and 21 % between strain AB14T and Haloterrigena turkmenica JCM 9101T. It is therefore proposed that strain AB14T represents a novel species, for which the name Haloterrigena saccharevitans sp. nov. is proposed. The type strain is AB14T (=AS 1.3730T=JCM 12889T).

scholarly journals Proposal of Lentzea deserti (Okoro et al. 2010) Nouioui et al. 2018 as a later heterotypic synonym of Lentzea atacamensis (Okoro et al. 2010) Nouioui et al. 2018 and an emended description of Lentzea atacamensis

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246533
Author(s):  
Mo Ping ◽  
Zhao Yun-Lin ◽  
Liu Jun ◽  
Gao Jian ◽  
Xu Zheng-Gang
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Relationship Of ◽  
Type Strains ◽  
The 16S Rrna Gene

The taxonomic relationship of Lentzea atacamensis and Lentzea deserti were re-evaluated using comparative genome analysis. The 16S rRNA gene sequence analysis indicated that the type strains of L. atacamensis and L. deserti shared 99.7% sequence similarity. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the genomes of two type strains were 88.6% and 98.8%, respectively, greater than the two recognized thresholds values of 70% dDDH and 95–96% ANI for bacterial species delineation. These results suggested that L. atacamensis and L. deserti should share the same taxonomic position. And this conclusion was further supported by similar phenotypic and chemotaxonomic features between them. Therefore, we propose that L. deserti is a later heterotypic synonym of L. atacamensis.

scholarly journals Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete

2005 ◽  
Vol 55 (3) ◽  
pp. 1329-1333 ◽  
Author(s):  
Wen-Jun Li ◽  
Yong-Guang Zhang ◽  
Yu-Qin Zhang ◽  
Shu-Kun Tang ◽  
Ping Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Rrna Gene ◽  
Qinghai Province ◽  
Light Yellow ◽  
Gene Sequence Analysis ◽  
Genotypic Analysis ◽  
Actinomycete Strain ◽  
The 16S Rrna Gene

An alkaliphilic actinomycete, strain YIM 80305T, which was isolated from a muddy sample in Chaka salt lake, Qinghai Province of China, was characterized using a polyphasic approach. The isolate produced light-yellow substrate and yellow–white aerial mycelia on most tested media. Optimum pH for growth was 9·0–10·0 with scant growth at pH 7·0. Results showed that strain YIM 80305T was obligately Na+-dependent, and showed sensitivity to K+. The DNA G+C content was 70·5 mol%. 16S rRNA gene sequence analysis together with these characteristics consistently assigned strain YIM 80305T to the genus Streptomyces. It formed a distinct clade based on analyses of the almost-complete and 120-nucleotide variable γ region of the 16S rRNA gene. It could be differentiated by phenotypic and genotypic analysis from all the Streptomyces species whose names have been validly published. On the basis of polyphasic evidence, Streptomyces sodiiphilus sp. nov. is proposed. The type strain is YIM 80305T (=CCTCC AA 203015T=CIP 107975T).

Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota

2021 ◽  
Vol 368 (18) ◽  
Author(s):  
Cheikh Ibrahima Lo ◽  
Niokhor Dione ◽  
Aminata Mbaye ◽  
Patricia Fernández-Mellado Gómez ◽  
Issa Isaac Ngom ◽  
...  
Keyword(s):  
Anaerobic Bacterium ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Average Nucleotide Identity ◽  
Fecal Flora ◽  
Rrna Gene Sequence ◽  
Human Gut ◽  
The 16S Rrna Gene

ABSTRACT Strain Marseille-P3519T isolated from the fecal flora of a 25-year-old healthy French woman was a Gram-positive anaerobic bacterium, non-motile and non-spore forming. The 16S rRNA gene sequence of Marseille-P3519 showed 97.73% of sequence similarity with Limosilactobacillus reuteri DSM 20016, the closest species, phylogenetically. Furthermore, the average nucleotide identity of strain Marseille-3519 with its closest related species was 75.8% that was very below the recommended threshold (>95–96%). Its genome had 2 237 367 bp with 45.42 mol% of G + C content. Major fatty acids were C16:0 (50.8%), C18:1n9 (18.0%), C18:2n6 (9.8%) and C19:1n9 (8.9%). It was catalase negative and fermented glycerol, glucose, fructose, D-maltose, lactose and mannose. These findings support that strain Marseille-P3519 ( = CSURP3519 = CECT 30110) is a new member of the genus Limosilactobacillus for which the name Limosilactobacillus caccae sp. nov., is proposed.

scholarly journals Haloarchaeobius iranensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline lake

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1021-1026 ◽  
Author(s):  
A. Makhdoumi-Kakhki ◽  
M. A. Amoozegar ◽  
M. Bagheri ◽  
M. Ramezani ◽  
A. Ventosa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Brine Sample ◽  
The Family ◽  
The 16S Rrna Gene

Strain EB21T was isolated from a brine sample from Aran-Bidgol salt lake, a saline playa in Iran. Strain EB21T was an orange–red-pigmented, motile rod and required at least 2 M NaCl but not MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, while it was able to grow at pH 6.0–8.0 and 25–55 °C. Analysis of the 16S rRNA gene sequence revealed that strain EB21T is a member of the family Halobacteriaceae , showing low levels of similarity to other members of the family. The highest sequence similarities, 91.8, 91.7 and 91.5 %, were obtained with the 16S rRNA gene sequences of the type strains of Halobiforma lacisalsi , Haloterrigena thermotolerans and Halalkalicoccus tibetensis , respectively. Polar lipid analyses revealed that strain EB21T contains phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Three unidentified glycolipids and one minor phospholipid were also observed. The only quinone present was MK-8(II-H2). The G+C content of its DNA was 67.7 mol%. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain EB21T is thus considered to represent a novel species in a new genus within the family Halobacteriaceae , order Halobacteriales , for which the name Haloarchaeobius iranensis gen. nov., sp. nov. is proposed. The type strain of Haloarchaeobius iranensis is EB21T ( = IBRC-M 10013T  = KCTC 4048T).

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