scholarly journals PREVALENCE OF SUBCLINICAL MASTITIS, ASSOCIATED RISK FACTORS AND ANTIBIOTIC SENSITIVITY OF ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS IN CATTLE IN MULTAN, PAKISTAN

2021 ◽  
Vol 37 (1) ◽  
pp. 48-55
Author(s):  
T. Ahmad ◽  
M. Kashif ◽  
E. Ahmad ◽  
M. Nadeem ◽  
M. Rizwan
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antibiotic Sensitivity ◽  
Subclinical Mastitis ◽  
E Coli ◽  
California Mastitis Test ◽  
Associated Risk Factors ◽  
And Staphylococcus Aureus ◽  
Important Disease ◽  
Teat Dipping

Mastitis is the most widespread and economically important disease of dairy sector worldwide. The present research was, therefore intended to establish the prevalence of mastitis in and around the Multan district. A total of 100 dairy cows were randomly sampled during the period from July 2018 to October 2018 from different local dairy farms at Multan. After screening with California Mastitis Test, milk samples from mastitic animals were cultured and subjected to microbiological examination.The overall prevalence of subclinical mastitis in cows was 38% and quarter-wise prevalence of subclinical mastitis was 36.20%.. Mastitis in front left quarters was 34.40%, right front quarters 31.60%, while left rare quarters 41% and right rear quarters 38%. Bacteria Staphylococcus, Streptococcus, Corynebacterium, Enterococci, Salmonella, Bacillus, Klebsiella, and Pseudomonas were isolated from infected samples. Staphylococcus aureus was the top-ranking isolated bacteria followed by Strept. Agalactiae and E. coli. The lack of proper milking techniques and no teat dipping were the most significantly associated risk factors. In the current study, E. coli isolates were sensitive to Gentamycine, Streptomycin, Chlororamphenicol and Kanamycin while resistant to Vancomycin and Penicillin. Similarly, S. aureus was extremely susceptible to Chloramphenicol, Vancomycin and Kanamycin but resistant to Tetracycline and Penicillin. It was concluded that subclinical mastitis was widely prevalent in Multan city. This study will help the farmers to adopt effective measures to control mastitis regarding the risk factors.

scholarly journals Prevalence, Risk Factors, and Antibiogram of Bacteria Isolated from Milk of Goats with Subclinical Mastitis in Thika East Subcounty, Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Precious Mahlangu ◽  
Naomi Maina ◽  
John Kagira
Keyword(s):  
Risk Factors ◽  
Significant Risk ◽  
Antibiotic Sensitivity ◽  
Subclinical Mastitis ◽  
Cross Sectional Study ◽  
Penicillin G ◽  
Dairy Goats ◽  
Cross Sectional ◽  
California Mastitis Test ◽  
Staphylococcus Intermedius

A cross-sectional study was carried out to determine the prevalence and risk factors of subclinical mastitis in dairy goats in Thika East Subcounty, Kenya. Further the bacterial pathogens and their antibiogram were investigated. Farm level data on risk factors were obtained from 41 farmers using questionnaires. Milk was obtained from 110 lactating dairy goats and tested for submastitis using California Mastitis Test (CMT). The prevalence of subclinical mastitis at goat level was estimated to be at 50.9% using CMT, out of which 86.5% yielded bacteria on culture. The significant risk factors associated with the occurrence of subclinical mastitis were cleaning schedule (p=0.022, OD=1.047) and parity of the goat (p=0048, OD=1.37). Higher prevalence of subclinical mastitis was observed for goats residing in houses cleaned at least once a fortnight. Does in the first parity were least affected. 169 bacterial isolates were obtained from culture, of which 52 isolates from major classes of isolated bacteria were tested for antibiotic sensitivity to six antibiotics. Fourteen different bacteria were isolated and identified from the milk samples. Coagulase-negative Staphylococci (20.7%), Serratia spp. (19.5%), Citrobacter spp. (16%), Klebsiella spp. (11%), Staphylococcus aureus (10.7%), Enterobacter spp. (6.5%), Escherichia coli (5.9%), Proteus spp. (3%), Corynebacterium spp. (1.8%), Morganella spp. (1.8%), Streptococcus spp. (1.2%), Providencia spp. (0.6%), Micrococcus spp. (0.6%), and Staphylococcus intermedius (0.6%) were isolated and identified from the samples. All the isolates were resistant to Penicillin G, while 98% of the isolates were sensitive to Streptomycin. In conclusion, the study showed that a large proportion of goats were affected by subclinical mastitis, with the main bacteria being Staphylococci spp. and coliforms, and that most of the tested antibiotics can be used in the treatment of mastitis. Farmers need to be trained on improved control of mastitis through adoption of good dairy husbandry and milking practices.

scholarly journals Genetic tools and techniques for fish improvement

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
G. Gopikrishna
Keyword(s):  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Threshold Values ◽  
E Coli ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Mastitic Milk ◽  
Tools And Techniques ◽  
Important Disease ◽  
Apparently Healthy

Mastitis is an economically important disease in goats. In the present study, a total of 100 quarter milk samples were collected from 50 apparently healthy Sirohi Goats in Udaipur district. The culture examination of these samples revealed the prevalence of subclinical mastitis in Sirohi goats as 26 (26/100) % and 34 (17/50) % on udder quarter basis and animal basis, respectively. The highest prevalence of subclinical mastitis was observed in 3rd lactation (50 %). Among the different isolates, staphylococci were found as most prevalent organism accounting for 37.50 (12/32) %, followed by streptococci 28.12 (9/32) %, E. coli 18.75 (6/32) %, bacilli 9.37 (3/32) % and Corynebacterium spp. 6.25 (2/32) %. The threshold values of modified California mastitis test, total somatic cell count and electrical conductivity in subclinical mastitic milk samples were observed to be +1, 1.0 million cells/ml of milk and 6.0 mS/cm, respectively.

scholarly journals Impact of Subclinical Mastitis-causing Bacterial Species on the Composition and Chemical Properties of Milk

2021 ◽  
Author(s):  
Tarik Safak ◽  
Ali Risvanli ◽  
Zulal Ascı-Toraman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Bacterial Species ◽  
Chemical Properties ◽  
Subclinical Mastitis ◽  
Milk Composition ◽  
Mineral Matter ◽  
California Mastitis Test ◽  
And Staphylococcus Aureus

Background: Here we investigate whether the composition and some chemical properties of milk can correlate with bacterial species causing subclinical mastitis in cows. Methods: One hundred and eighty cows were used in the study. The California Mastitis Test (CMT) was applied to the selected cows. The cows were divided into four groups: cows with negative CMT (n = 45), the Escherichia coli (n = 45), Streptococcus agalactiae (n = 45) and Staphylococcus aureus (n = 45) groups. Result: Milk composition and some chemical properties were measured. The highest fat ratio (5.82±0.34%) was found in the Escherichia coli group (p less than 0.05). The proportions of solid-non-fat (9.67±0.09%), protein (3.51±0.03%), lactose (5.29±05%) and mineral matter (0.75±0.01%) were lowest in the Escherichia coli group (p less than 0.05). The electrical conductivity of milk was lowest in the CMT-negative group (4.23±0.02 mS/cm), while the Streptococcus agalactiae group had the highest value (4.61±0.02 mS/cm) (p less than 0.05). The specific gravity of the Escherichia coli group was lower than the CMT-negative (1,034.91±0.56 kg/m3) and Staphylococcus aureus (1,034.24±0.65 kg/m3) groups (p less than 0.05). Based on our findings, we propose that milk composition and some chemical parameters are altered in subclinical mastitis. However, these could not be standardized according to the responsible bacterial species. We recommend that these parameters are monitored regularly on dairy farms.

scholarly journals Investigation of prevalence and risk factors of subclinical mastitis of dairy buffaloes at Bhola district of Bangladesh

2021 ◽  
Vol 6 (4) ◽  
pp. 697-704
Author(s):  
Hasan Aliul ◽  
Paul Ashit Kumar ◽  
Riad Mohammad Mahmood ◽  
Rahman Mizanur ◽  
Ahmed Md Selim
Keyword(s):  
Risk Factors ◽  
Management Practices ◽  
Subclinical Mastitis ◽  
Control Measures ◽  
Lactation Period ◽  
California Mastitis Test ◽  
Lactating Buffaloes ◽  
Associated Risk Factors ◽  
On Farm

The subclinical mastitis is more serious and is responsible for much greater loss to the dairy industry in Bangladesh. This study was performed to determine the prevalence of subclinical mastitis and identify the associated risk factors in randomly selected 200 lactating buffaloes (192 local and 8 crossbred) at some selected regions of Bhola district, Bangladesh during the period from March 2018 to February 2019. California Mastitis Test (CMT) was performed on each quarter milk sample of lactating buffaloes at field condition for the determination of subclinical mastitis in dairy buffaloes. Overall prevalence of subclinical mastitis was 10.50% (21/200). The prevalence of subclinical mastitis (SCM) was comparatively higher in crossbred (12.5%) as compared to local breed (10.42%). The highest prevalence of SCM in dairy buffaloes was also found in late lactation period (12.82%), age group 7 to 18 years (13.46%), rainy season (11.1%) and 2nd parity group (10.74%). Moreover, the buffaloes graze in free range areas; large and medium sized farms; poor health conditions and other diseases of post parturient period increasing the susceptibility of SCM. For early diagnosis of subclinical mastitis, CMT can be performed regularly as a control measures and emphasis should be provided on farm management practices; particularly on milking hygiene and udder sanitation. Asian J. Med. Biol. Res. December 2020, 6(4): 697-704

scholarly journals Clinical and subclinical mastitis in cows

2005 ◽  
Vol 21 (1-2) ◽  
pp. 29-39 ◽  
Author(s):  
Slavca Hristov ◽  
Branislav Stankovic ◽  
Renata Relic
Keyword(s):  
Serum Albumin Level ◽  
Antibiotic Sensitivity ◽  
Subclinical Mastitis ◽  
Similar Rate ◽  
Albumin Level ◽  
Similar Data ◽  
E Coli ◽  
California Mastitis Test ◽  
Specific Pathogen ◽  
Klebsiella Spp

The most important aspects of diagnosis and occurrence of clinical and subclinical mastitis are presented in this paper. Mastitis is inflammation of udders parenchyma characterized by pathological changes, and physical chemical and bacteriological changes of milk. Subclinical mastitis could be diagnosed by direct or indirect somatic cell count (SCC) by California Mastitis test and N-acetyl-B-D-glucosamynidasys test, ELISA and Whiteside test, and also by chloride and bovine serum albumin level, as well as by electric conductivity of milk. Therapy of mastitis is more successful with appropriate information about pathogen, which demands special mediums laboratory conditions and stuff, in order to evaluate antibiotic sensitivity of specific pathogen. In the majority of countries, similar rate of morbidity (about 40%) and infection rate (25%) of dairy cows were reported, regardless of the cause. Prevalence investigations show similarity in the most of the countries. Predominant cause of mastitis is Staphylococcus aureus, followed by Streptococcus agalactiae. Available data show that incidence rate of mastitis caused by E. coli, Pseudomonas aeruginosa, Aerobacter aerogenes and Klebsiella spp. is increasing. Similar data on incidence of mastits and prevalence of specific pathogen were established in our country.

scholarly journals Prevalence of Caprine Subclinical Mastitis in Mymensingh Area, Bangladesh and Characterization of Associated Bacterial Agents and the Risk Factors

2013 ◽  
Vol 1 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Khandkar Muhammad Alimul Razi ◽  
Md Bahanur Rahman ◽  
Gerardo H Flores-Gutiérrez ◽  
Md Tanvir Rahman
Keyword(s):  
Risk Factors ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Farming System ◽  
Subclinical Mastitis ◽  
Lactation Period ◽  
Isolation And Identification ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Sensitivity Pattern

The prevalence of caprine subclinical mastitis in Mymensingh area was assessed by California Mastitis Test (CMT). Milk samples were also analyzed for the isolation and identification of bacterial agents that might be associated with caprine subclinical mastitis including their antibiotic sensitivity pattern against commonly used antibiotics. In addition, the influ-ence of certain risk factors such as age of animal, parity, length of lactation period, type of farming system and type of housing floor, on the prevalence of caprine subclinical mastitis was also evaluated. Milk samples were collected from 59 goats (113 udder halves). The overall prevalence of caprine subclinical mastitis as determined by CMT was 18.64%. On an udder half basis the prevalence was 15.04%. Certain risk factors associated with caprine subclinical mastitis were identified. The prevalence was higher in older animals, with greater parity and longer lactation period. The prevalence was also higher in farms where goats were raised under traditional farming system with earthen floors. The predominant bacterial species isolated from milk that were collected from the subclinical mastitis positive cases were Staphylococcus aureus, S. epidermidis and Bacillus subtilis, that showed various degrees of sensitivity to the antibiotics used in the study.DOI: http://dx.doi.org/10.3329/mh.v1i1.13705 Microbes and Health Vol.1(1) June 2012 pp.1-5

Studies on Subclinical Mastitis in Sirohi Goats in Southern Rajasthan with Reference to Prevalence and Diagnostic Aspects

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
S. K. Sharma ◽  
Hitesh K. Meena ◽  
Joshi Monika
Keyword(s):  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Threshold Values ◽  
E Coli ◽  
Diagnostic Aspects ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Mastitic Milk ◽  
Important Disease ◽  
Apparently Healthy

Mastitis is an economically important disease in goats. In the present study, a total of 100 quarter milk samples were collected from 50 apparently healthy Sirohi Goats in Udaipur district. The culture examination of these samples revealed the prevalence of subclinical mastitis in Sirohi goats as 26 (26/100) % and 34 (17/50) % on udder quarter basis and animal basis, respectively. The highest prevalence of subclinical mastitis was observed in 3rd lactation (50 %). Among the different isolates, staphylococci were found as most prevalent organism accounting for 37.50 (12/32) %, followed by streptococci 28.12 (9/32) %, E. coli 18.75 (6/32) %, bacilli 9.37 (3/32) % and Corynebacterium spp. 6.25 (2/32) %. The threshold values of modified California mastitis test, total somatic cell count and electrical conductivity in subclinical mastitic milk samples were observed to be +1, 1.0 million cells/ml of milk and 6.0 mS/cm, respectively.

scholarly journals Disparity in Microbiological Pattern of Cauti in Precisely Tribal Patients in Relation to Impacting Factors from the Known Pattern in the Medical Intensive Care Unit in a Tribal Tertiary Care Centre

2021 ◽  
Author(s):  
Monalisa Subudhi ◽  
PAT Jagatheeswary ◽  
Sudhanshu Kumar Das ◽  
Khetrabasi Subudhi ◽  
Rashmiranjan Rout
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Intensive Care ◽  
Medical Intensive Care Unit ◽  
Tertiary Care ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
E Coli ◽  
Medical Intensive Care ◽  
And Staphylococcus Aureus

The important risk factor for the development of CAUTI, especially in the intensive care units, is the presence, method and length of Duration of urinary catheterization. The other potential significant risk factors are gender, age, uncontrolled diabetes and long hospital stay. There have been no studies on Tribal patients describing the factors (Demographic as well as Risk factors) which influence the deviation in microbiological pattern of CAUTI in of the MICU, from the patients of other area studied till now. This prospective study was conducted over Tribal patients aged ≥ 18 years who developed features of symptomatic urinary tract infection following an indwelling urinary Foley’s catheter more than 48 hours, in the Medical Intensive Care Unit. Geographical and Clinical data were collected. The CAUTI rate in 33 Tribal patients was 19.2 per 1000 device days with an incidence of 17.3% in the MICU. The microbiological trend was Staphylococcus aureus 12 (26.0%), E. coli 11 (23.9%), Candida albicans 9 (19.5%), Klebsiella pneumoniae 5 (10.8%), CONS 3 (6.5%), Streptococcus pneumoniae 3(6.0%), Pseudomonas aeruginosa 2 (4.3%) and Acinetobacter baumannii 1 (2.1%). Staphylococcus aureus was the common cause of CAUTI in low and medium socioeconomic tribal patients of more than 20 to 40 years of age, as compared to E. coli in Nontribal Patients. E.coli was found in the dominated female patients and in age upto 20 years and more than 40to 60 years old patients and Klebsiella pneumonae in above 60 years age. High status group with Acinetobactor baumannii. Candida albicans was associated with use of urinary catheter less than 7 days. Length of ICU stay more than 14 days was associated with E. coli and Staphylococcus aureus in equally proportionately. CONS 2(50%) was found in type-2 Diabetes mellitus and Staphylococcus aureus 7 (53.8 %) with Sickle cell anemia in Tribal patients. Our analysis precisely of this Tribal patients, brings several important and unique findings, which will not only aid in the development of some new or update guidelines but also encourage the researcher to do more study, for the prevention of CAUTI in the MICU of Tribal tertiary care centre.

scholarly journals Assessment of sub-clinical mastitis and its associated risk factors in dairy livestock of Lamjung, Nepal

2013 ◽  
Vol 2 (2) ◽  
pp. 49-54 ◽  
Author(s):  
T Khanal ◽  
A Pandit
Keyword(s):  
Risk Factors ◽  
Management Practices ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cross Sectional Study ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
California Mastitis Test ◽  
Dairy Animals ◽  
Associated Risk Factors

INTRODUCTION: Mastitis is one among the top three threats faced by dairy farmers. The study was carried out to assess sub-clinical mastitis, management practices and associated risk factors for mastitis. MATERIALS AND METHODS: A cross sectional study was conducted in Chandreshwor and Archalbot VDCs of Lamjung district taking 63 dairy livestock randomly each from a herd along with questionnaire survey to respective owner. Tem ml of milk sample from each quarter was taken in a sterilized syringe for further laboratory investigation. California Mastitis Test (CMT) was performed at farmer’s shed. Organisms were identified based on colony characteristics, Gram's staining and various biochemical tests. RESULTS: On CMT, subclinical mastitis was 46.1% (n=29) and 30.15% (n=76) on animal and quarter basis respectively, however, culture showed, 28.6% and 24.2%. Streptococcal mastitis was the commonest (11.1%) followed by coliform (9.5%) and staphylococcal (7.9%). Mastitis was highest in left fore quarter (34.92%) followed by left hind (31.76%), right hind (28.57%) and right fore (25.39%). Coliform & Staphylococcal mastitis was highest in left fore and right hind quarter respectively. Most of dairy animals (86%) were on zero grazing, 30% (n=19) of the farmers had forage trees and only 29% (n=18) had known about subclinical mastitis. The average milk production was 3.5 ±1.47 liters. Subclinical mastitis was associated significantly (p<0.01) with livestock yielding more than 3 lt per lactation. CONCLUSIONS: There was high prevalence of subclinical mastitis in dairy livestock at Lamjung due to poor management, unhygienic shed, and little knowledge on subclinical mastitis.  DOI: http://dx.doi.org/10.3126/ijim.v2i2.8322 Int J Infect Microbiol 2013;2(2):49-54  

scholarly journals Subclinical mastitis in cattle in Algeria: Frequency of occurrence and bacteriological isolates

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Radhwane Saidi ◽  
Djamel Khelef ◽  
Rachid Kaidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Frequency Of Occurrence ◽  
E Coli ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Wide Range ◽  
Mycoplasma Spp

The present study was carried out to determine the prevalence of subclinical mastitis in cattle in eighteen herds in the center region of Algeria. Milk samples were collected from 560 quarters of 140 cows free of clinical mastitis. The samples were subjected to California Mastitis Test (CMT) and the positive samples were analysed by bacteriological culture and Speed ​​Mam® Color. The overall quarter prevalence was 28.77% whilst animal prevalence was 28.57%.Bacteriological analysis showed that there was a wide range of bacteria that cause these infections. Staphylococcus aureus (40%) was found to be the most prevalent organism followed by Streptococcus spp. (12.5%), Enterobacteriaceae (2.5%), Pseudomonas spp. (2.5%), Staphylococcusaureus + Streptococcus spp. (12.5%), Streptococcus spp.+ Escherichia coli (7.5%), S. aureus + Mycoplasma spp.(7.5%), and S. aureus +Streptococcus spp.+ E. coli (5%).

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