STUDYING THE ANTIMICROBIAL EFFECT OF DETERGENTS AND DISINFECTANTS ON THE MICROFLORA OF THE EGG SHELL SURFACE

2020 ◽  
pp. 248-257
Author(s):  
S. Gordynets ◽  
L. Charniauskaya ◽  
J. Yakhnovets ◽  
T. Hovzun
Keyword(s):  
Staphylococcus Aureus ◽  
Microbial Contamination ◽  
Antimicrobial Effect ◽  
Hand Washing ◽  
Pathogenic Microorganisms ◽  
Shell Surface ◽  
Manual Treatment ◽  
Egg Shells ◽  
Egg Shell ◽  
Ammonium Compounds

The article presents the results of evaluating the antimicrobial effect of detergents and disinfectants on the microflora of the eggshell surface. Washing and disinfection of eggs was carried out by manual treatment by immersion in washing and disinfecting solutions and subsequent washing with brushes. For washing eggs, an alkaline detergent with a «Sanet BIO» disinfectant effect was used, and for disinfection, one of the four selected disinfectants belonging to different groups of drugs: «SUNWAY DES» disinfectant (peroxide), «Silversil Des» disinfectant (silver-containing), «Lanex» disinfectant (based on Quaternary ammonium compounds), and «Catelon 502» disinfectant (containing peracetic acid). It was found that the treatment of eggs in order to reduce the contamination of the surface of their shells with «SUNWAY DES», «Catelon 502» and «Lanex» disinfectants by hand washing confirmed their effectiveness and leads to the disinfection of the shell from opportunistic and pathogenic microorganisms, as well as reduces the overall microbial contamination. Sanitization of egg shells of edible hen eggs by hand washing with the use of «Silversil Des» disinfectant in the spent modes showed that this tool is not effective in disinfecting egg shells, since immediately after processing, Staphylococcus aureus, Salmonella s.p.p. and Listeria s.p.p. bacteria were found on their surface.

A STUDY OF METHODS TO ENUMERATE THE MICROBIAL FLORA OF THE AVIAN EGG SHELL1

1973 ◽  
Vol 36 (2) ◽  
pp. 101-102 ◽  
Author(s):  
K. W. B. Gunaratne ◽  
John V. Spencer
Keyword(s):  
Pseudomonas Fluorescens ◽  
Microbial Flora ◽  
Shell Surface ◽  
Entire Surface ◽  
Significant Difference ◽  
Egg Shells ◽  
Egg Shell ◽  
Avian Egg

Experiments were done to compare results from three different methods used to enumerate the microbial flora of avian egg shells. The methods compared were: (a) swabbing the shell surface, (b) rinsing the shell surface, and (c) blending the entire shell and membranes after removal of the contents. Sanitized eggs held at 25 C were inoculated with a 24-hr old culture of Pseudomonas fluorescens by immersion for 5 min at 25 C. In the swab method, the entire surface of the inoculated egg was swabbed by rolling the swab in either direction twice as each area was swabbed. For the rinse method, the inoculated egg was held using a metal holder and rinsed with 100 ml of 0.1% peptone solution. With the blender method, the entire emptied inoculated egg shell and shell membranes were blended using a Sorvall Omni-Mixer to which a presterilized Mason jar was attached. As expected, the blender method gave significantly higher counts (P<0.05) than either the swab or rinse methods. There was no significant difference (P<0.05) between counts obtained by the swab and rinse methods.

INFLUENCE OF DETERGENTS AND DISINFECTANTS ON THE QUALITY INDICATORS OF EDIBLE HEN EGGS WASHED AND DISINFECTED

2020 ◽  
pp. 258-269
Author(s):  
S. Gordynets ◽  
L. Charniauskaya ◽  
J. Yakhnovets ◽  
S. Kosyanenko ◽  
A. Kiselev
Keyword(s):  
Control Sample ◽  
Storage Period ◽  
Control Group ◽  
Organoleptic Evaluation ◽  
Air Chamber ◽  
Egg Density ◽  
Egg Shells ◽  
Egg Shell ◽  
Ammonium Compounds ◽  
Experimental Group

The article presents the results of evaluating the effect of detergent and various disinfectants used for sanitary treatment of the surface of egg shells of hen edible on their quality and safety indicators. Four disinfectants belonging to different groups of drugs were selected for research: «SUNWAY DES» (peroxide), «Silversil Des» (silver-containing), «Lanex» (quaternary-ammonium compounds), «Catelon 502» (containing peracetic acid). A decrease of the mass of all samples of eggs during the whole storage period (35 days) 5,6–6,6% of initial, was increasing the height of the air chamber eggs 2,3–2,9 times, with the largest increase was in specimens treated by «Silvercel Des» and «Lanex». The decrease in egg density in salt solution was at the same level in all experimental samples. There was a decrease in the yolk index by 7,7% in the control sample and by 16,3–19,0% in the experimental samples, but the yolk shell did not break in all cases. By the end of the shelf eggs life, the shell thickness and elastic deformation met the requirements. According to microbiological indicators, all egg samples during 33 days of storage met the requirements of the technical normative legal acts. Organoleptic evaluation of eggs at 16 and 35 days of storage allowed us to establish that the best, closest in organoleptics to the eggs of the control group, were the eggs of the 1st experimental group, and the worst, according to the conclusion of the majority of tasters, were the eggs of the 3rd experimental group. Based on a comprehensive analysis of the research, it was found that «SUNWAY DES» and «Catelon 502» disinfectants can be recommended for disinfecting the surface of the egg shell of edible hen eggs.

SEM observations on egg-shell of the Japanese tusser, Antheraea yamamai and Chinese tusser,Antheraea pernyi

1990 ◽  
Vol 48 (3) ◽  
pp. 98-99
Author(s):  
Hu Cui ◽  
Jian Hong ◽  
Gao Oikang ◽  
Wu Xiaojiang ◽  
Ye Gongyin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Surface Structure ◽  
Shell Surface ◽  
Antheraea Pernyi ◽  
Egg Shells ◽  
Egg Shell ◽  
Scanning Electron

The egg-shell surface structure of the Japanese tusser and Chinese tusser was observed by means of scanning electron microscopy. There were a lot of similarities between the two egg-shells, but the fine structure may be easily distinguished. As to the Japanese tusser, the petals of the petaloid pattern around micropyle were elongate and raised in the middle (Fig. 1); micropylar tubes numbered 11-13 (Figs. 2 and 3); the wider and thicker bank formed irregularly shaped net-like structure of the egg-shell surface other than in the vicinity of micropyle (Fig. 5); and the thickness of the egg-shell was about 70 μm. In the Chinese tusser the petals were shorter, wider,and even; micropylar tubes numbered 8-9 (Fig. 4); the narrow and low bank formed hexagonal, pentagonal, heptagonal, or octagonal net-like structure; the aeropyle wall was well developed, almost the same in size (Figs. 8 and 9); and the thickness of the egg-shell was about 40 μm.

scholarly journals Study of the Antimicrobial effect of Melia azedarach L. plant

2013 ◽  
Vol 7 (1) ◽  
pp. 87-90
Author(s):  
Suad M Majeed
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Leaf Extract ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Melia Azedarach ◽  
Pathogenic Microorganisms ◽  
Leaf Extracts ◽  
Aqueous Leaf Extract ◽  
Agar Well Diffusion Method

This study was aimed to evaluate the antimicrobial activity of the leaf extracts of Melia azedarach L. plant against different pathogenic microorganisms such as (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, klebsilla sp., Candida albicans). Two plant extracts (Aqueous and ethanolic) under four different concentrations (25, 50, 75, 100) mg/ml were used by Agar-well diffusion method. Chemical detection of extract showed that the extracts contain tannins, flavonoids, terpins, steriods, alkaloids, and saponins. Aqueous leaf extract showed no effect against all tested microorganisms at (25, 50, 75, 100) mg/ml concentration except Candida albicans which was sensitive to 100mg/ml concentration. While Ethanolic (80%) leaf extract showed sensitivity on Staphylococcus aureus at 100mg/ml concentration.

Antimicrobial effect of some essential oils on Staphylococcus aureus in minced meat

2016 ◽  
Vol 30 (1) ◽  
pp. 183-191
Author(s):  
Hemmat Ibrahim ◽  
Rasha El Sabagh ◽  
Nahla Abou El-Roos ◽  
Hend Abd El Fattah
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Antimicrobial Effect ◽  
Minced Meat

NOTES ON CERTAIN BUTTERFLIES, THEIR HABITS, ETC

1882 ◽  
Vol 14 (3) ◽  
pp. 49-56
Author(s):  
W. H. Edwards
Keyword(s):  
New Born ◽  
Egg Shells ◽  
Egg Shell ◽  
The Way

8. On Young Caterpillars Eating their Egg Shells.Mr. Scudder, Butterflies, p. 101, says, after describing the way in which the caterpillar eats out of the egg: “The taste he has gained of egg-shell seems to allure him; for, strange as it may seem, although placed by the provident parent within immediate reach of choice and succulent food, he will not taste it until he has devoured the last remmant of his prison-walls. Strange food this for a new born babe! The act, however, is plainly a provision of nature by which the tender animal is rid of a sure token to his enemies of his immediate proximity.” Surely here is an error in fact, and a wrong conclusion whatever the fact may be. I read the above statement on the 25th July last, and at once went to my garden to search for eggs of Libythea Bachmanni, on Hackberry leaves. The young caterpillars of this species are green, of a shade so near that of the leaves they feed on, that it is very difficult to discover them. Even where the tip of the leaf has been eaten, and their presence is suspected, it is easy to overlook them. I found at once three eggs and one young caterpillar. The egg from which this caterpillar had come was present at the base of the leaf on the extreme tip of which the little creature rested. A hole was in its side near the top, and no more had been eaten than just enough to permit egress.

scholarly journals Antimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albicans

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1480
Author(s):  
William Chiappim ◽  
Aline da Graça Sampaio ◽  
Felipe Miranda ◽  
Mariana Fraga ◽  
Gilberto Petraconi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Tap Water ◽  
Antimicrobial Effect ◽  
Gliding Arc ◽  
Oxidation Reduction ◽  
Treatment Conditions ◽  
Oxidation Reduction Potential

In this study, the potential antimicrobial activity of plasma-activated tap water (PAW) was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans. For this, PAW was prepared in a gliding arc plasma system using two treatment conditions: stagnant water and water stirring by a magnetic stirrer, called moving water. Subsequently, their oxidation-reduction potential (ORP), pH, electrical conductivity (σ), and total dissolved solids (TDS) were monitored in different areas of the sample divided according to the depth of the beaker. It was observed that PAW obtained in dynamic conditions showed a more uniform acidity among the evaluated areas with pH 3.53 and ORP of 215 mV. Finally, standardized suspensions of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10799), and Candida albicans (SC 5314) were treated with PAW, and the reduction of viable cells determined the antimicrobial effect. Our results indicate that the tap water, activated by plasma treatment using gliding arc, is an excellent inactivation agent in the case of Staphylococcus aureus and Escherichia coli. On the other hand, no significant antimicrobial activity was achieved for Candida albicans.

scholarly journals Effect of barium loading on CaO derived from waste egg shell heterogeneous catalyst for canola oil biodiesel

2018 ◽  
Vol 192 ◽  
pp. 03006
Author(s):  
Jakkrapong Jitjamnong
Keyword(s):  
Microwave Irradiation ◽  
Heterogeneous Catalyst ◽  
Canola Oil ◽  
Catalytic Performance ◽  
Biodiesel Production ◽  
Catalyst Loading ◽  
Impregnation Method ◽  
Egg Shells ◽  
Infrared Spectrometer ◽  
Egg Shell

The purpose of this research was to investigate the catalytic activity of Ba loading on calcium oxide (CaO) catalyst by varying the amount of barium added during the synthesis: 5-15 wt%. The waste egg shells were utilized as a CaO heterogeneous catalyst by calcined at 900 °C for 2 h. The Ba/CaO catalysts were prepared by impregnation method and were used as a catalyst in transesterification reaction of canola oil via microwave irradiation under microwave power 300 W. The characterization of catalyst and FAME composition of biodiesel were determined by X-ray fluorescence (XRF), scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), and gas chromatography (GC-FID). The conditions of biodiesel production were operated at 60 °C, 3 wt% of catalyst loading, 9:1 methanol-to-canola oil ratio, and microwave irradiation power was 300W for 2 min. The experimental results found that, the waste egg shells consist mainly of CaCO3, which was decomposed to CaO more than 88 wt% after cacination step. The 15 wt% Ba/CaO catalysts exhibited the best catalytic performance with the FAME conversion higher than 97.68%.

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