scholarly journals Novel phytotherapy for tinea versicolor by extracting zingiber wightianum thwaites

2021 ◽  
pp. 143-150
Author(s):  
Ms.Sini Baby ◽  
Dimal Jose ◽  
Agnes K Mathew ◽  
Biniya John ◽  
Antony Varghese Antony
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Side Effects ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Soxhlet Extraction ◽  
Economic Benefits ◽  
Malassezia Furfur ◽  
Phytochemical Screening ◽  
Tinea Versicolor

Herbal medicines are gaining more interest both in terms of medicinal and economic benefits and they are getting more patient compliance due to its fewer side effects as compared to allopathic medicines. The present research was aimed at phytochemical screening and antifungal evaluation of rhizomic extract of Zingiber wightianum Thwaites belonging to Zingiberaceae family. The rhizomes were subjected for Soxhlet extraction and the preliminary phytochemical screening of this extract proved the presence of alkaloids, carbohydrates, tannins, flavonoids, saponins, phenols. Subsequently, these extracts were evaluated for antifungal activity and minimum inhibitory concentration was determined. Hence revealed the potential to treat a fungal infection Tinea versicolor, caused by fungus Malassezia furfur. This investigation was also undertaken with a view to formulate and evaluate the herbal gel using Zingiber wightianum rhizomic extract. Thus, the overall studies concluded that the rhizomic extract of Zingiber wightianum Thwaites and its formulation would be a breakthrough in the field of modern antifungal drug.

Phytochemical Screening and Antifungal Activity of Kulantrillo De Allambre (Adiantum capillus-veneris) Extract as a Formulated Vaginal Cream Against Candida albicans

10.17158/234 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Kathleen G. Bersabal ◽  
Kyrie Marie D. Deluao ◽  
Meg C. Ibarra ◽  
Nessa Karla C. Jerusalem
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Physical Properties ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Antifungal Property ◽  
Vaginal Cream ◽  
Adiantum Capillus Veneris

Candida albicans is a fungus that is normally present on the skin and in mucous membranes such as vagina, mouth, or rectum. This fungus also can travel through the blood stream and affect the throat, intestines, and heart valves. It becomes an infectious agent when there is some change in the body environment that allows it to grow out of control; thus, causing a modern day disease brought on by antibiotics, drugs, processed foods, sugar and poor diet. Folkloric practice suggests that Kulantrillo de Allambre (Adiantum capillus-veneris) is effective as antifungal medication against Candida albicans; therefore, this study was undertaken to verify such claim. This effort included the phytochemical screening of active constituents, susceptibility and minimum inhibitory concentration tests involving the plant. To prove that Kulantrillo de Allambre possesses antifungal activity, a formulation of vaginal cream out of the plant extracts was tested. Alkaloids, saponins, steroids, flavonoids, tannins and anthraquinones were performed following the method described by Guevara et al. (2005). Kirby-Bauer Method was employed for the susceptibility test and two-fold agar dilution was employed for the MIC. Vaginal cream was formulated following the method of Ansel (2004). Tests revealed the presence of flavonoids only, which exhibit the antifungal property. Kirby-Bauer Method revealed that both test extract and positive control showed strong inhibition equivalent to 17.71 ± 6.3183 mm and 23.54 ± 4.9629 mm, respectively. Minimum Inhibitory Concentration of the plant extract was 50,000 ug/mL. Statistical analysis showed that there is no significant difference between the antifungal property of plant extract and positive control. Both test drugs have similar antifungal property, suggesting that the plant material is a good antifungal agent. The physical properties of vaginal cream conformed to the standard of physical properties of commercialized product available in the market.

scholarly journals EVALUATION OF PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT AND ANTIFUNGAL ACTIVITY OF PITHECELLOBIUM DULCE LEAVES’ EXTRACT

2016 ◽  
Vol 10 (1) ◽  
pp. 370 ◽  
Author(s):  
Suman Kumari
Keyword(s):  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Side Effects ◽  
Inhibitory Concentration ◽  
Water Extract ◽  
Pathogenic Fungi ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Solvent Fractionation

ABSTRACTObjective: Diseases due to pathogenic fungi represent a critical problem to human health which is a major cause of morbidity and mortalityworldwide. Synthetic drugs such as Amphotericin B mitigate many of the side effects associated with liver and kidney dysfunctions. Widely distributedtraditional medicinal plants-based antimicrobial drugs are cost effective in the treatment of infectious diseases having no side effects. Pithecellobiumdulce (Roxb.) belongs to the family of Leguminosae, is an evergreen tree widely distributed in the greater part of India which has been widely used intraditional medicine, but lacks scientific scrutiny. The present study revealed P. dulce extract effectiveness against A. fumigates and Aspergillus nigerand showed antioxidant activity.Methods: Leaves’ extract was prepared according to the polarity of solvent, i.e., hexane, chloroform, acetone, methanol, and water. Phytochemicalanalysis, antioxidant activity, and minimum inhibitory concentration (MIC) were tested against selected fungus.Results: Phytochemical analysis of the extract revealed the presence of alkaloids, flavonoids, saponins, coumarin, tannins, anthocyanin, andtriterpenoids. Methanol and water extract was found to contain significant amounts of alkaloids, phenols, and flavonoids. Methanolic extract showedMIC against tested fungus, and extract was further fractionated by solvent-solvent fractionation and MIC was tested. MIC for A. fumigatus was0.62 mg/ml and for A. niger was 1.25 mg/ml, and the results were comparable with effective synthetic drug Amphotericin B.Conclusion: Extract possesses potent fungicidal activity which, in turn, may be due to the presence of biologically active ingredient.Keywords: Antioxidant activity, Phytochemical activity, Antifungal activity, Minimum inhibitory concentration, Fractionation.

scholarly journals Two Symmetrical Squarylium Cyanine Dyes: Synthesis, Photophysics and Antifungal Activity in Saccharomyces cerevisiae

2020 ◽  
Vol 3 (1) ◽  
pp. 106
Author(s):  
Vanessa S. D. Gomes ◽  
João C. C. Ferreira ◽  
Renato E. F. Boto ◽  
Paulo Almeida ◽  
Maria João M. F. Sousa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Emission Spectroscopy ◽  
Inhibitory Concentration ◽  
Analytical Techniques ◽  
Cyanine Dyes ◽  
Absorption And Emission ◽  
Nir Absorption ◽  
Absorption And Emission Spectroscopy

Two squarylium cyanine dyes were synthesized and characterized by the usual analytical techniques, including Vis-NIR absorption and emission spectroscopy. Their antifungal activity was evaluated, through the obtention of minimum inhibitory concentration (MIC) values, using yeasts of the species Saccharomyces cerevisiae as a biological model.

ANTIFUNGAL TOPICAL GEL OF LEAF EXTRACT OF AMARANTHUS VIRIDIS L. FOR TREATMENT OF CUTANEOUS CANDIDIASIS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 39-44
Author(s):  
A. P. Pandit ◽  
◽  
K. S. Khandagale ◽  
V. C Nakhate ◽  
N. N Dharmadhikari
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
High Viscosity ◽  
Good Choice ◽  
Zone Of Inhibition ◽  
Minimum Fungicidal Concentration ◽  
Topical Gel ◽  
Cutaneous Candidiasis

The objective of the study was to prepare antifungal gel using leaves of Amaranthus viridis for the treatment of cutaneous candidiasis. The leaves were studied for pharmacognostic evaluation. The powder of leaves was tested for phytoconstituents. The plant extract was evaluated for the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and antifungal activity. Gel was prepared and evaluated for pH, viscosity, homogeneity and grittiness. MIC and MFC of extract were both found to be 600 mg. The zone of inhibition of extract was obtained at 21.2±0.2mm, which confirmed antifungal activity, due to presence of phenolic compound. Gel exhibited good antifungal activity, good spreadability, extrudability and high viscosity. Thus, gel loaded with leaves extract of A. viridis is a good choice for the treatment of cutaneous candidiasis.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

In vitro antifungal activity of luliconazole against nondermatophytic moulds

2019 ◽  
Vol 58 (5) ◽  
pp. 703-706 ◽  
Author(s):  
Jun Maeda ◽  
Hiroyasu Koga ◽  
Kou Yuasa ◽  
Daisuke Neki ◽  
Yasuko Nanjoh ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Drug Resistant ◽  
Fusarium Spp ◽  
Microdilution Method ◽  
In Vitro Antifungal Activity

Abstract In vitro antifungal activity of luliconazole against nondermatophytic moulds causing superficial infections was compared with that of five classes of 12 topical and systemic drugs. The minimum inhibitory concentration (MIC) of the drugs against the genera of Neoscytalidium, Fusarium, Aspergillus, Scedosporium, and Alternaria was measured via modified microdilution method. In results, the nondermatophytic moulds were found to be less susceptible to drugs to which Neoscytalidium spp. and Fusarium spp. were typically drug resistant. However, luliconazole was effective against all the genera tested, including afore-mentioned two species, and had the lowest MICs among the drugs tested.

scholarly journals Inclusion Complexes of Concentrated Orange Oils and β-Cyclodextrin: Physicochemical and Biological Characterizations

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5109
Author(s):  
Cynthia Torres-Alvarez ◽  
Sandra Castillo ◽  
Eduardo Sánchez-García ◽  
Carlos Aguilera González ◽  
Sergio Arturo Galindo-Rodríguez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inclusion Complexes ◽  
Inhibitory Concentration ◽  
Entrapment Efficiency ◽  
Ft Ir ◽  
Ir Analysis

Concentrated orange oils (5x, 10x, 20x) are ingredients used in different industries as components of flavors and aromas due to their great organoleptic qualities. This research focuses on the search for alternative uses for their application through encapsulation in inclusion complexes with β-cyclodextrin (β–CD). Inclusion complexes of concentrated orange oils (COEO) and β–CD were developed by the co-precipitated method in ratios of 4:96, 12:88, and 16:84 (w/w, COEO: β–CD). The best powder recovery was in the ratio 16:84 for the three oils, with values between 82% and 84.8%. The 20x oil in relation 12:88 showed the highest entrapment efficiency (89.5%) with 102.3 mg/g of β–CD. The FT-IR analysis may suggest an interaction between the oil and the β–CD. The best antioxidant activity was observed in the ratio 12:88 for the three oils. The antifungal activity was determined for all the inclusion complexes, and the 10x fraction showed the highest inhibition at a concentration of 10 mg/mL in ratios 12:88 and 16:84. Antibacterial activity was determined by the minimum inhibitory concentration (MIC) and was found at a concentration of 1.25 mg/mL in ratios 12:88 and 16:84 for 5x and 20x oils.

scholarly journals Antifungal Activity of Essential Oil ofEucalyptus camaldulensisDehnh. against SelectedFusariumspp.

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Martin Muthee Gakuubi ◽  
Angeline W. Maina ◽  
John M. Wagacha
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Inhibitory Concentration ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Minimum Fungicidal Concentration ◽  
Potential Use

The objective of this study was to evaluate the antifungal activity of essential oil (EO) ofEucalyptus camaldulensisDehnh. against fiveFusariumspp. commonly associated with maize.The essential oil had been extracted by steam distillation in a modified Clevenger-type apparatus from leaves ofE. camaldulensisand their chemical composition characterized by gas chromatography mass spectrometry. Poisoned food technique was used to determine the percentage inhibition of mycelial growth, minimum inhibitory concentration, and minimum fungicidal concentration of the EO on the test pathogens. Antifungal activity of different concentrations of the EO was evaluated using disc diffusion method. The most abundant compounds identified in the EO were 1,8-cineole (16.2%),α-pinene (15.6%),α-phellandrene (10.0%), and p-cymene (8.1%). The EO produced complete mycelial growth inhibition in all the test pathogens at a concentration of 7-8 μL/mL after five days of incubation. The minimum inhibitory concentration and minimum fungicidal concentration of the EO on the test fungi were in the range of 7-8 μL/mL and 8–10 μL/mL, respectively. These findings confirm the fungicidal properties ofE. camaldulensisessential oils and their potential use in the management of economically importantFusariumspp. and as possible alternatives to synthetic fungicides.

scholarly journals Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Fresh Water Fish ◽  
Minimum Fungicidal Concentration ◽  
Kaempferia Galanga ◽  
Activity Test

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.

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