scholarly journals Economically viable isolation and characterization of oleanolic acid from Eugenia caryophyllus (Spreng.)

2020 ◽  
Vol 48 (1) ◽  
Author(s):  
Nilesh B. Banarase ◽  
◽  
Chanchal D. Kaur ◽  
Keyword(s):  
Oleanolic Acid ◽  
Dry Matter ◽  
Raw Materials ◽  
Cost Effective ◽  
Nmr Studies ◽  
Pharmacological Activities ◽  
Isolation And Characterization ◽  
Viable Method ◽  
Selection Of

Oleanolic acid is an interesting molecule found widespread throughout the plant kingdom with promising pharmacological activities. However, the utilization of oleanolic acid in the modern therapy is quite difficult due to the lack of proper isolation method and selection of commercially available raw materials. In the present study, the new economically viable method for the isolation of oleanolic acid from flower buds of Eugenia caryophyllus (Spreng.), commonly known as clove bud, was developed. The yield of oleanolic acid was found to be 1.8 g/ 100 g dry matter (1.8%). The isolated oleanolic acid was characterized by DSC, TLC, FTIR, ESI-HRMS, and NMR studies. The purity of isolated oleanolic acid was determined by HPLC studies, and it was found to be 97.7% pure. The method so developed is simple and cost-effective with industrial applicability.

scholarly journals Measurement system for over-the-air evaluation of UHF RFID tags quality

2016 ◽  
Vol 4 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Riccardo Colella ◽  
Luca Catarinucci ◽  
Luciano Tarricone
Keyword(s):  
Radio Frequency Identification ◽  
Cost Effective ◽  
Automatic Identification ◽  
Rfid Tags ◽  
Uhf Rfid ◽  
Wireless Power ◽  
Measurement Systems ◽  
Frequency Identification ◽  
Selection Of

Radio-frequency identification (RFID) technology is a consolidated example of wireless power transfer system in which passive electromagnetic labels called tags are able to harvest electromagnetic energy from the reader antennas, power-up their internal circuitry and provide the automatic identification of objects. Being fully passive, the performance of RFID tags is strongly dependent on the context, so that the selection of the most suitable tag for the specific application becomes a key point. In this work, a cost-effective but accurate system for the over-the-air electromagnetic characterization of assembled UHF RFID tags is firstly presented and then validated through comparison with a consolidated and diffused measurement systems. Moreover, challenging use-cases demonstrating the usefulness of the proposed systems in analyzing the electromagnetic performance of label-type tags also when applied on different material or embedded into concrete structures have been carried out.

scholarly journals Isolation and Characterization of Secondary Metabilites and Evaluation of Antimicrobial, Antioxidant and Thrombolytic Potentials of Erythrina variegata L. Bark

2019 ◽  
Vol 31 (8) ◽  
pp. 1842-1846
Author(s):  
Fatema Tabassum ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Mehedi Masud ◽  
Md. Imran Nur Manik ◽  
Monira Ahsan
Keyword(s):  
Methanolic Extract ◽  
Authentic Sample ◽  
Stem Bark ◽  
Moderate Activity ◽  
Zone Of Inhibition ◽  
Nmr Studies ◽  
Erythrina Variegata ◽  
Isolation And Characterization ◽  
Ic50 Value

Six compounds, sitosta-4-en-3-one (1), 3β,28-dihydroxyolean-12-en (2), scandenone (3), alpinum isoflavone (4), stigmasterol (5) and lupeol (6) were isolated from the methanol soluble extract of the stem bark of Erythrina variegata. The structure of the compounds was established by extensive NMR studies as well as co-TLC with authentic sample. The petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions of the methanolic extract of Erythrina variegata were evaluated for antimicrobial, antioxidant and thrombolytic properties. In the antimicrobial study, most of the fractions of the extract exhibited mild to moderate antimicrobial activity where the zone of inhibition were ranging from 7.69 to 19.51 mm. The chloroform soluble fraction showed significant antioxidant activity with IC50 value 66.28 μg/mL as compared to standard BHT (IC50 value 23.09 μg/mL). The thrombolytic property of different extracts of E. variegata exhibited moderate activity ranging from 31.25 to 57.78 %.

Seeds of Mung Bean (Vigna radiata (L.)R.Wilczek): Taxonomy, Phytochemistry, Medicinal Uses and Pharmacology

2020 ◽  
Vol 16 ◽  
Author(s):  
Sonali V. Uppalwar ◽  
Rohit Dutt
Keyword(s):  
Bioactive Compounds ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Antioxidant Treatment ◽  
Pharmacological Activities ◽  
Medicinal Uses ◽  
Isolation And Characterization ◽  
Potential Benefits ◽  
Food Grain

Background: Seeds of Mung bean (Vignaradiata (L.)R.Wilczek)is usually identified as a 'Green pearl' of Asia. It has been extensively used as a traditional food in the whole world. This is the best source of protein, minerals, and vitamins. Methods: Literature has been collected through SciFinder, Web of Science, Google Scholar, Pubmed, and a library. This review shares updated information on the botany, distribution, health benefits, phytochemistry and pharmacology of Mung bean seeds. Result: As per the literature survey, Its found that Mung seeds (Vigna radiata (L.) R.Wilczek) has a pharmacological activity such as anticancer, antihyperlipidemic, antihypertensive, antidiabetic, anti-microbial, antioxidant, treatment of alcoholism, reducing obesity, increasing muscular strength ,rheumatism, piles, liver, and neuro- diseases. This curative potential provides various beneficial outcomes in the field of research and increasing scientific interest in the identification of bioactive compounds responsible for various pharmacological activities. It is used in industries like pharmaceutical, food, and Cosmetics. Conclusion: Existing literature authenticates the potential benefits of Mung bean seeds (Vigna radiata (L.) R.Wilczek) from nutritional as well as medicinal perspective. This food grain need to be explored for identification, isolation, and characterization of a bioactive compounds against varied ailments.

scholarly journals Meat starter cultures: Isolation and characterization of lactic acid bacteria from traditional sausages

2019 ◽  
Vol 12 (1) ◽  
pp. 54-69 ◽  
Author(s):  
É. Laslo ◽  
É. György ◽  
A. Czikó
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Raw Materials ◽  
Meat Products ◽  
Starter Cultures ◽  
Probiotic Properties ◽  
Meat Industry ◽  
Bacterial Isolates ◽  
Isolation And Characterization

Abstract Fermented meat products represent an important segment of our alimentation. Obtaining these products is based on beneficial microorganism activity. In the case of traditional food products, these are commercial starters or autochthonous microflora. Fermentation of raw materials is mainly done by sugar metabolization of lactic acid bacteria (LAB). In addition, these microorganisms can have other beneficial properties too such as probiotic properties, antimicrobial compound production abilities, etc. In order to meet consumer demands, starter cultures are continuously developed to produce high-quality, healthy, and tasty products, thus contributing to guaranteeing microbiological safety and to improving one or more sensory characteristics, technological, nutritional, or health properties of the fermented products. The aim of our research is to determine the technological properties of autochthonous lactic acid bacteria originated from commercial fresh sausages in order to select and use them as potential starter cultures in the meat industry. In our work, we determined the relevant characteristics (such as salt tolerance, proteolytic activity, antimicrobial activity, and antibiotic resistance) of bacteria isolated from 16 fresh sausages. Based on our results, the studied bacterial isolates originated from sausages could be potentially used as autochthonous meat starter cultures.

scholarly journals SCREENING OF ASPERGILLUS FUNGI FOR EXTRA CELLULAR PROTEASE AND COLLAGENASE PRODUCTION

REPORTS ◽  
2020 ◽  
Vol 2 (330) ◽  
pp. 36-40
Author(s):  
R.K. Blieva ◽  
A.K. Kalieva ◽  
Zh.B. Suleimenova ◽  
A.S. Zhakipbekova ◽  
I.E. Tapenbayeva
Keyword(s):  
Proteolytic Enzymes ◽  
Raw Materials ◽  
Meat Industry ◽  
Improve Quality ◽  
Meat Processing ◽  
Enzyme Preparations ◽  
Technological Processes ◽  
Fungal Association ◽  
Selection Of

Protease and collagenase are the most important enzymes used for the processing of meat raw materials. In the meat industry, proteolytic enzymes are used to accelerate the maturation of meat and increase its yield. The use of enzyme preparations in meat processing makes it possible to rationally use meat raw materials, intensify technological processes, improve quality and expand the range of products. Collagenase, unlike protease, acts on those connective proteins of meat raw materials that determine its stiffness, breaking down hard-hydrolyzable and non-digestible collagen. The aim of this study was selection of strains of industrially valuable micromycetes from the collection of micromycetes that have the ability to synthesize extracellular protease and collagenase and create a fungal association. A comparative characterization of 7 strains of micromycetes of the genus Aspergillus and Penicillium - potential producers of protease and collagenase enzymes, was carried out. A. awamori 16 and A. awamori 22 showed the highest clearance zones and was used for further studies. The clearance zones of casein of A. awamori 16 on day 5 were 22.8 mm, and collagen 20.8 mm, while the clearance zones of casein of A. awamori 22 were 20.1 mm, and collagen - 19.1 mm.

scholarly journals Isolation and characterization of a transfectant influenza B virus altered in RNA segment 6

1999 ◽  
Vol 80 (9) ◽  
pp. 2353-2359 ◽  
Author(s):  
Kate V. Rowley ◽  
Ruth Harvey ◽  
Wendy S. Barclay
Keyword(s):  
Helper Virus ◽  
Influenza B Virus ◽  
Influenza B ◽  
System A ◽  
Isolation And Characterization ◽  
B Virus ◽  
Infectious Cycle ◽  
Selection Of

This report describes the successful generation of an influenza B transfectant virus altered in RNA segment 6, which encodes the neuraminidase (NA) protein. The procedure for selection of the transfectant virus relies on the use of strain-specific anti-NA monoclonal antibodies to inhibit growth of the helper virus within the system. A transfectant virus has been engineered which has a coding change in the NA protein. This change resulted in attenuated growth in vitro that could be rescued by addition of exogenous bacterial NA. The mutant virus-associated NA activity was unstable as a result of the engineered changes. The ability to genetically manipulate influenza B virus segment 6 will allow us to assess the function of both NA and the small protein NB, also coded from this RNA, within the context of the virus infectious cycle.

scholarly journals Isolation of Triterpenoid Glycoside from Bark ofTerminalia arjunausing Chromatographic Technique and Investigation of Pharmacological Behavior upon Muscle Tissues

2007 ◽  
Vol 4 (4) ◽  
pp. 474-479 ◽  
Author(s):  
Tanushree Patnaik ◽  
R. K. Dey ◽  
Panchanan Gouda
Keyword(s):  
Organic Compounds ◽  
Cost Effective ◽  
Spectroscopic Technique ◽  
Terminalia Arjuna ◽  
Chromatographic Technique ◽  
Isolation And Characterization ◽  
Triterpenoid Glycoside ◽  
Muscle Tissues ◽  
Isolated Compound

This study reports the isolation and characterization of a new triterpenoid glycoside extracted from the bark ofTerminalia arjuna. The isolation of the organic compounds was done using simple chromatographic technique. Compound characterization using various spectroscopic technique identify the final isolated compound as Olean-3β,22β-diol-12-en-28β-D-glucopyranoside-oic acid. The method of isolation is simple, cost effective and efficient. The preliminary bioactivity of the compound was also evaluated.

scholarly journals Isolation and Characterization of Oleanolic Acid Benzoate from the Ethylacetate Leaves Extracts of Vernonia ambigua (Kotschy Ex. Peyr)

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
U. Z. Gwandu ◽  
S. M. Dangoggo ◽  
U. Z. Faruk ◽  
E. M. Halilu ◽  
A. J. Yusuf ◽  
...  
Keyword(s):  
Oleanolic Acid ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Isolation And Characterization ◽  
The Family ◽  
Annual Herb ◽  
Intestinal Worm ◽  
Layer Chromatography ◽  
Leaf Part

Vernonia ambigua, an annual herb which belongs to the family Asteraceae/Compositae has been used in ethnomedicine to treat different ailments such as fungal infection, diarrhea and intestinal worm among others. The aim of the study was to isolate and characterize compound(s) from the leaves of V. ambigua. Powdered leaf of the plant were gradiently extracted with n-hexane, ethylacetate and methanol using Soxhlet extractor. The ethyl acetate extract was gradiently eluted in a silica gel column and further purified using preparative thin-layer chromatography which led to the isolation of colourless solid substance identified as Oleanolic acid benzoate via chemical tests, 1D-NMR analysis and by comparison with reference spectral data. After thorough exploration of the leaf extract of V. ambigua, using available techniques, it can be concluded that the leaf part of the plant contains a chemical compound suggested to be oleanolic acid benzoate.

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