scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 7 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS

2020 ◽  
pp. 39-42
Author(s):  
SWETA KUMARI ◽  
VIRENDRA VAISHNAV
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
High Efficiency ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Cinnamomum Tamala ◽  
Medicinal Plant Extracts

Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli. Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC). Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria. Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.

scholarly journals THE FUNCTIONAL CHARACTER OF Auricularia auricula CRUDE POLYSACCHARIDES: ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY

2020 ◽  
Vol 19 (3B) ◽  
Author(s):  
Rizki Rabeca Elfirta ◽  
Iwan Saskiawan
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Functional Food ◽  
Hot Water ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Auricularia Auricula ◽  
Potential Source

The food stuffs can be clasified as functional food since the foods can improve the human health. One of them are the food stuffs which have function as antioxidant and antibacterial. These activities were studied on crude polysaccharides from Auricularia auricula. The sample was subsequently isolated using hot water and 1 M NaOH to obtain water and alkali soluble of crude polysaccharides. The antioxidant activity was evaluated using β-caroten-linoleat assay. The results showed that the alkali soluble of crude polysaccharides had the highest antioxidant activity (85.82%) at 350 μg/ml. The water and alkali soluble of crude polysaccharides from A.auricula were evaluated for their antibacterial activity using disc diffusion method. The alkali soluble of crude polysaccharides was found to have the highest antibacterial activity at 100 mg/ml against Staphylococcus aureus InaCC B4 and Escherichia coli InaCC B5 with clear zone values of 3.18 mm and 5.10 mm, respectively. The findings indicated that the alkali soluble of crude polysaccharides from A. auricula could potentially be used in part of well-balanced diets and could be considered as a potential source of natural antioxidant and antibacterial products.  

scholarly journals Uji Aktivitas Antimikroba Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol 96% Terhadap Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 41-44
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Staphylococcus Aureus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Traditional Medicines ◽  
Paper Disc

ABSTRAKStaphylococcus aureus merupakan bakteri yang relatif sering dijumpai pada manusia. Beberapa infeksi yang disebabkan oleh Staphylococcus aureus antara lain, bisul, infeksi luka dan pneumonia. Salah satu obattradisional yang dapat digunakan untuk mengobati infeksi yang disebabkan oleh Staphylococcus aureus adalah jamur lingzhi (Ganoderma lucidum). Penelitian dilakukan dengan 5 konsentrasi ekstrak etanol jamur lingzhi, yaitu kontrol negatif (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml dan 100 µg/ml dan dilakukan 6 kali pengulangan. Metode yang digunakan dalam uji aktivitas antimikroba yaitu metode difusi kertas cakram. Data zona hambat dianalisis dengan uji Anova One-Way. Hasil penelitian menunjukkan bahwa ekstrak etanol jamurlingzhi (Ganoderma lucidum) dengan konsentrasi 100 µg/ml memiliki rata-rata daya hambat tertinggi yaitu sebesar 18,0 mm (sangat aktif). Dapat disimpulkan bahwa semakin besar konsentrasi ekstrak jamur lingzhi (Ganoderma lucidum), maka akan berpengaruh besar pula terhadap zona hambat bakteri Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.ABSTRACTStaphylococcus aureus is a bacteria that can cause infections , such as boils , wound infections and pneumonia. One of the traditional medicines that can be used to treat these infections are fungal lingzhi (Ganodermalucidum). The study was conducted with five concentrations of negative control (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml, and 100 µg/ml with six repeats. The method used in the test of antimicrobial activity is paper disc diffusion method. Data of inhibition zone were analyzed by Anova-One Way test. The results showed that the ethanol extract of lingzhi mushroom (Ganoderma lucidum) at concentration 100 µg/ml has highest average of inhibitory regions as big as 18,0 mm. It can be concluded that the greater the concentration of extract of lingzhi mushroom (Ganoderma lucidum) , it will affect the greater the inhibition zone against Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN PANGI (Pangium edule Reinw. ex Blume) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Pseudomonas aeruginosa

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 275
Author(s):  
Glorya Sakul ◽  
Herny E. I. Simbala ◽  
Gerald Rundengan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
North Sulawesi ◽  
Staphyloccocus Aureus ◽  
Inhibitory Strength

ABSTRACTOne of the natural ingredients that are often used as a medicinal plants is Pangi (Pangium edule Reinw. Ex Blume) plant. The part of pangi that are often used are leaves, which is known have antibacterial activity. North Sulawesi people empirically use this plant as food and to cure various diseases such as treating itching on the skin caused by bacteria found on the skin. The aim of this research was to determine and study the strength of antibacterial inhibition based on the category of inhibition by Davis and Stout of ethanol extract from pangi (Pangium edule Reinw. ex Blume) leaf performed by disc diffusion Kirby and Bauer method againts Staphylococcus aureus, Escherichia coli and Pseudomonos aeruginosa bacterias. The result showed that the ethanol extract from pangi (Pangium edule Reinw. Ex Blume) leaf has medium inhibitory strength at concentrations of 4%, 6% and 8%.Keywords: Pangium edule Reinw. ex Blume leaf, Antibacterial, Disc diffusion method  ABSTRAKSalah satu bahan alam yang sering digunakan sebagai tumbuhan obat adalah tumbuhan pangi (Pangium edule Reinw. ex Blume). Bagian dari tumbuhan pangi yang sering digunakan adalah bagian daun, dimana telah diketahui mempunyai aktivitas antibakteri. Masyarakat Sulawesi Utara secara empiris menggunakan tumbuhan ini sebagai bahan makanan serta untuk menyembuhkan berbagai penyakit seperti mengobati penyakit gatal-gatal pada kulit yang disebabkan oleh bakteri yang terdapat pada kulit. Penelitian ini bertujuan untuk mengetahui kekuatan daya hambat antibakteri berdasarkan kategori penggolongan daya hambat oleh Davis dan Stout dari ekstrak etanol daun Pangium edule Reinw. ex. Blume dengan metode difusi agar (difusi disk Kirby dan Bauer) terhadap bakteri uji Staphyloccocus aureus, Escherichia coli dan Pseudomonas aeruginosa. Hasil penelitian didapati bahwa ekstrak etanol daun Pangium edule Reinw ex. Blume mempunyai kekuatan daya hambat kategori sedang pada konsentrasi 4%, 6% dan 8%.Kata kunci: Daun Pangium edule Reinw. ex Blume, Antibakteri, Metode difusi disk

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