scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

scholarly journals ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT

2018 ◽  
Vol 5 (1) ◽  
pp. 51 ◽  
Author(s):  
Edy Fachrial ◽  
Harmileni Harmileni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Palm Oil ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

<p><em>The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.</em></p>

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals Screening of the antimicrobial activity of marine sponge extracts from Martinique

2021 ◽  
Author(s):  
Julie Piron ◽  
Jessica Pastour ◽  
Niklas Tysklind ◽  
Juliette Smith-Ravin ◽  
Fabienne Priam
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Marine Sponges ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

scholarly journals Antibacterial Activity of Cissus quadrangularis Stem Extract on the Pathogenic and Industrial Waste Watered Bacteria

2020 ◽  
pp. 28-38
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Industrial Waste ◽  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Cissus Quadrangularis

Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem widespread throughout Bangladesh. The in vitro antimicrobial activity of C. quadrangularis extracts was studied against selected pathogenic bacteria, industrial wasted bacteria, and broth dilution assay. The most commonly used method of microbiological assay is the disc diffusion method. C. quadrangularis stem extracted with four solvents (Petroleum spirit, methanol, ethyl acetate, and dichloromethane) were tested for antimicrobial activities against some pathogenic microorganisms Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and some industrial (Tannery, Tobacco, and Sugar mill) waste watered bacteria by disc diffusion method. Among the four extracts, ethyl acetate showed moderate antibacterial activity against X. campestris (004-1) and industrial watered bacteria. But, the commercial disc Oxicycline doesn’t show any antibacterial activity against the industrial waste watered bacteria. Petroleum spirit, methanol, and dichloromethane extract were ineffective against all of the tested bacteria.

scholarly journals Antimicrobial Properties of 6-Bromoeugenol and Eugenol

2016 ◽  
Vol 53 ◽  
pp. 57-64
Author(s):  
Radia Mahboub ◽  
Faiza Memmou
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

scholarly journals Antioxidant and antimicrobial effect of alkaloid bulbs extract of Polianthes tuberosa L. (Amaryllidaceae) cultivated in Algeria

2020 ◽  
Vol 10 (4) ◽  
pp. 44-48
Author(s):  
Nihed BARGHOUT ◽  
Nada CHEBATA ◽  
Saida MOUMENE ◽  
Seddik KHENNOUF ◽  
Abdelaziz GHARBI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Scavenging Effect ◽  
Alkaloid Extracts

Polianthes tuberosa L. (Amaryllidaceae) is an ornamental and medicinal plant. Its flowers and bulbs are used traditionally as a diuretic, emetic, against rashes and gonorrhea. The aim of this work was, to evaluate the antioxidant and antimicrobial activities of bulbs and bulbils alkaloid extracts of P. tuberosa. Antiradical effect was assessed against DPPH radical. However, antimicrobial activity was measured through the disc diffusion method against Escherichae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Staphylococcus aureus resistant to Methicillin (MRSA) and Candida albicans ATCC 90028. The scavenging effect against DPPH showed that the bulbs and bulbils alkaloids extracts exhibited an antiradical effect with IC50 = 0.231±0.017 mg/mL and 0.233±0.093 mg/mL respectively, less than the effect of vitamin C with IC50 = 0.0194±0.0002 mg/mL. Antimicrobial activity results reveal that both alkaloid bulbs extracts at 50 mg/mL did not have any inhibitory effect against the studied strains using the disc diffusion method. According to this work, bulbs and bulbils alkaloid extracts show a moderate antioxidant effect; that could be recommended as a natural antioxidant. Although tuberose bulbs were used traditionally as a soap substitute; bulbs alkaloid extract has no antimicrobial effect. Keywords: Polianthes tuberosa L., bulbs, bulbils, alkaloids, antiradical activity, antimicrobial activity.

scholarly journals Antibacterial Activities of Some Schiff Bases Involving Thiosemicarbazide and Ketones

2014 ◽  
Vol 26 ◽  
pp. 53-61 ◽  
Author(s):  
S.M.S. Shariar ◽  
M. Jesmin ◽  
M.M. Ali
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Schiff Bases ◽  
Cytotoxic Effect ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Three Schiff bases derived from thiosemicarbazide and ketones (Vanillin, Benzophenone and Acetophenone) were used to study their antibacterial activities against some pathogenic bacteria by disc diffusion method. Of these, benzophenone thiosemicarbazone showed significant antibacterial activity as compared with that of Kanamycin. All these three compounds were found to possess cytotoxic effect. Minimum inhibitory concentration of these compounds was also determined.

scholarly journals AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA”

2019 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Aloysius Aloysius ◽  
Anjurniza Ulfa ◽  
Anggita Kasih Fianti Situmorang ◽  
Harmileni Harmileni ◽  
Edy Fachrial
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Fermented Food ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Gram Staining ◽  
Potential Source

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.

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