scholarly journals IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS

2020 ◽  
pp. 39-42
Author(s):  
SWETA KUMARI ◽  
VIRENDRA VAISHNAV
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
High Efficiency ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Cinnamomum Tamala ◽  
Medicinal Plant Extracts

Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli. Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC). Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria. Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

2018 ◽  
Vol 34 (5) ◽  
pp. 2495-2501 ◽  
Author(s):  
Sarmd D. Noori ◽  
Mazin N. Mousa ◽  
Shaker A. N. Al-Jadaan
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Beta Lactam ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nur Azfa Shuib ◽  
Anwar Iqbal ◽  
Fatimatul Akmal Sulaiman ◽  
Izzatie Razak ◽  
Deny Susanti
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disc Diffusion ◽  
Flavonoid Content ◽  
Disc Diffusion Method ◽  
Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

In Vitro Evaluation of Antifungal Activity of Three Traditionally Used Medicinal Plants; Umbilicus Intermedius Boiss, Cuminum Cyminum and Zingiber Officinale Extracts

2019 ◽  
Author(s):  
Ameneh Takesh ◽  
Mahnaz Fatahinia ◽  
Ali Zarei Mahmoudabadi
Keyword(s):  
Antifungal Activity ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Cuminum Cyminum ◽  
Medicinal Plant Extracts ◽  
Alternaria Sp ◽  
Medium Method

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods.  Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium spp., Mucor spp., Stemphylium spp., Drechslera spp., Alternaria spp., Cladosporium spp., and Aureobasidium pullulans. Furthermore, 17 candida isolates including, C. albicans, C. glabrata and C. dubliniensis were tested. In the present study two methods of disc diffusion method, agar wells diffusion method were used for assay. Then, the mixing with culture medium method was used for assessment of the antifungal activity of extracts against Alternaria sp.(as black mold), A. terreus (as hyaline mold) and C. albicans (as yeast) to compare methods as well. Results: No fungi were susceptible to extracts in disc diffusion method and agar wells diffusion method. But, this study showed that in mixing with culture medium method, cumin extract has valuable anti-fungal property and Umbilicus intermedius boiss has the inhibitory properties against the black fungi. Furthermore, it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods. Alternaria sp. and C. albicans were susceptible and resistant to all extracts.  Conclusions: it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods and inhibitory potency of the extracts varies according to the type of extraction and their concentration.

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