scholarly journals Nano selenium treatment effects on thyroid hormones, immunity and antioxidant status in rabbits

2019 ◽  
Vol 27 (2) ◽  
pp. 93 ◽  
Author(s):  
Sherif Yousif Eid ◽  
Hussein Mustafa El-Zaher ◽  
Sana Sayed Emara ◽  
Omar Abdel-Hamed Farid ◽  
Michael Ibrahim Michael
Keyword(s):  
Liver Tissue ◽  
Positive Influence ◽  
Control Groups ◽  
Thyroid Activity ◽  
Live Body Weight ◽  
Selenium Treatment ◽  
Total Antioxidant ◽  
Serum Aspartate Aminotransferase ◽  
And Control ◽  
Gpx Activity

<p>The present study was conducted to compare the effect of Nano-Selenium (Nano-Se) and sodium selenite (SSe) on antioxidant enzyme activity, immunity and thyroid activity of growing New Zealand White (NZW) rabbits. In this study, 72 male rabbits (5 wk old) were divided randomly into 3 groups (24 rabbits each). The first group served as a placebo; in groups 2 and 3, each rabbit was intramuscularly injected once a week with 4 mL solution of Nano-Se or SSe, respectively, for a 2-mo period. The solution was adjusted to provide 30 μg Se/kg/live body weight. Results showed that Nano-Se treatment significantly (<em>P</em>&lt;0.0001) increased in superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) more than control, but decreased significantly each of glutathione disulphide (GSSG) and nitric oxide (NO) levels in serum. Likewise, supplementation of SSe increased (<em>P</em>&lt;0.0001) GPx activity and significantly decreased both malondialdehyde (MDA) and GSSG levels more than control. Nano-Se significantly enhanced serum IgG and IgM more than SSe and control groups. Serum aspartate aminotransferase increased (<em>P</em>&lt;0.0001) due to Nano-Se treatment as compared to control and SSe, although the lowest (<em>P</em>&lt;0.0001) activity of alanine aminotransferase was recorded due to SSe supplementation. Nano-Se treatment increased (<em>P</em>&lt;0.0001) both T3 and T4 concentrations more than other groups. Furthermore, administration of Nano-Se increased SOD, GPx, GSH, total antioxidant capacity (TAC) and adenosine triphosphate (ATP) in liver tissue of growing rabbits, while it decreased MDA and 8-hydroxy-2’deoxyguanosine (8-oHdG) levels in liver tissue compared with control. Also, SSe showed an increase (<em>P</em>&lt;0.0001) in GSH, and ATP, but significantly decreased TAC and MDA levels compared with control. It can be concluded that Nano-Se supplementation significantly enhanced the activity of antioxidant enzymes in both serum and liver tissues, with a greater positive influence on immunoglobulin production and thyroid activity in growing NZW rabbits than SSe.</p>

scholarly journals Impact of Ileal Transposition Surgical Intervention on Antioxidant Status Measured in Liver Tissue of Obese Zucker Rats (Crl:ZUC-Leprfa)

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Bronisława Skrzep-Poloczek ◽  
Dominika Stygar ◽  
Tomasz Sawczyn ◽  
Ewa Romuk ◽  
Elżbieta Chełmecka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Tissue ◽  
Antioxidant Status ◽  
Zucker Rats ◽  
Control Group ◽  
Ileal Transposition ◽  
Control Groups ◽  
Sham Procedure ◽  
Total Antioxidant ◽  
And Control

Background. The main factor characteristic for low-grade systemic inflammation typical for obesity is oxidative stress (OS). Reactive oxygen species (ROS) production is higher and more increased in time in the obese patients than in lean subjects. Aims. To assess the effect of ileal transposition (IT) and sham types of bariatric procedures on the antioxidative systems in the liver tissue of Zucker rats (Crl:ZUC Leprfa). Method. 21 animals were divided into the experimental groups: control group (n=7), sham group (n=7), and IT group (n=7). Sham and IT animals underwent selected surgery. The concentration of total antioxidant capacity (TAC), total antioxidant status (TOS), and activity of glutathione reductase (glutathione-disulfide reductase, GR, GSR), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total superoxide dismutase activity (SOD) were assessed in liver tissue 3 months after surgery. Results. IT procedure significantly increased TAC when compared to sham and the control group. Animals after IT showed higher levels of TOS when compared to sham procedure. The total amount of TOS was similar in IT and control groups. GPx activity was increased in the groups submitted to the sham and IT surgery in relation to control. GR and CAT activities were significantly higher after IT in comparison to control and sham procedures. Total SOD and MnSOD were significantly higher in sham-operated animals in comparison to IT intervention and control groups. Conclusions. IT procedure had a positive impact on the diminishing of oxidative stress measured by TAC and TOS markers. The dynamic, adaptive, and protective mechanisms of enzymatic antioxidant systems were observed after the IT but not sham procedure. Nevertheless, 3 months after surgery, the midterm effect of bariatric surgery was observed, which might not fully balance the antioxidative response.

scholarly journals The Influence of ProbioticLactobacillus caseiin Combination with Prebiotic Inulin on the Antioxidant Capacity of Human Plasma

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Paulina Kleniewska ◽  
Arkadiusz Hoffmann ◽  
Ewa Pniewska ◽  
Rafał Pawliczak
Keyword(s):  
Antioxidant Capacity ◽  
Human Plasma ◽  
Antioxidant Properties ◽  
Positive Influence ◽  
Reducing Ability ◽  
Plasma Antioxidant Capacity ◽  
Synbiotic Supplementation ◽  
Plasma Antioxidant ◽  
And Control ◽  
Gpx Activity

The aim of the present study was to assess whether probiotic bacteriaLactobacillus casei(4 × 108 CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n=32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containingL. caseiplus Inulin resulted in a significant increase in FRAP values (p=0.00008) and CAT activity (p=0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containingL. casei(4 × 108 CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes.

scholarly journals Influence of Increased Dietary Selenium on Glutathione Peroxidase Activity and Glutathione Concentration in Erythrocytes of Lactating Sows

2014 ◽  
Vol 14 (4) ◽  
pp. 869-882 ◽  
Author(s):  
Pavel Horký
Keyword(s):  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Glutathione Peroxidase Activity ◽  
Control Groups ◽  
Large White ◽  
Large White Breed ◽  
Lactating Sows ◽  
White Breed ◽  
And Control ◽  
Gpx Activity

Abstract We studied the influence of different selenium (Se) levels on some parameters of the antioxidant potential of lactating sows. The experiment involved 15 sows of the Large White breed divided into 3 equal groups. The addition of Se (from Se-enriched yeast) amounted to 0.0, 0.25 and 0.50 mg per kg of diet in the control, Se1 and Se2 groups, respectively. Erythrocyte Se concentration, glutathione peroxidase (GPx) activity and reduced/oxygenated glutathione ratio were measured as some of the indicators of the sow’s antioxidant status. Supplementation of Se at 0.25 mg/kg of diet increased Se concentration (by 50.0%; P<0.01), GPx activity (by 17.0%: P<0.05) and reduced/ oxygenated glutathione ratio (by 29.7%; P<0.05). Addition of Se at 0.50 mg/kg of diet increased Se concentration (by 63.6%; P<0.01), GPx activity (by 34.7%; P<0.01) and reduced/oxygenated glutathione ratio (by 64.0%; P<0.01). A significant relationship (P<0.01) was found between the Se concentrations and activity of GPx in erythrocytes from sows in the Se1 and Se2 groups (r=0.76 and 0.77, respectively). Percentage of piglets weaned/born was higher (P>0.05) in the Se2 than in the Se1 and control groups. In summary, addition of 0.25 or 0.50 mg Se/kg of diet can improve antioxidant status of lactating sows, but addition of 0.25 mg Se/kg may be also sufficient.

scholarly journals Evaluation of the T-SPOT.TB test, oxidation-related factors, and antimicrobial peptide LL-37 in the diagnosis of pulmonary tuberculosis with type 2 diabetes

2021 ◽  
Vol 49 (12) ◽  
pp. 030006052110644
Author(s):  
Liangliang Ma ◽  
Xuelin Chen ◽  
Shengfeng Weng ◽  
Xinting Yang
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Pulmonary Tuberculosis ◽  
Antimicrobial Peptide ◽  
Diagnostic Value ◽  
Control Groups ◽  
Related Factors ◽  
Total Antioxidant ◽  
And Control

Objective To investigate the diagnostic value of the T cell spot (T-SPOT.TB) test, oxidation-related factors (ORF), and antimicrobial peptide LL-37 in patients with pulmonary tuberculosis (PTB) with type 2 diabetes. Methods A total of 560 patients with PTB admitted to our hospital from January 2019 to April 2021 were retrospectively included in this study. Of these, 232 patients with PTB and type 2 diabetes were assigned to the combined group, and 328 patients without complications were assigned to the PTB group. Results Areas under the curve (AUCs) for the number of spot-forming cells in CFP10 and ESAT-6 test panels detecting PTB with type 2 diabetes were 0.892 (95% confidence interval [CI] 0.831–0.921) and 0.893 (95% CI 0.841–0.935), respectively. CFP10 combined with ESAT-6 had the highest diagnostic value, with sensitivity and specificity levels and an AUC of 87.73%, 88.93%, and 0.942 (95% CI 0.907–0.967), respectively. The levels of total antioxidant capacity, superoxide dismutase, and catalase in the combined group were lower than in PTB and control groups. Conclusion The combination of T-SPOT.TB, ORF, and LL-37 in the diagnosis of pulmonary tuberculosis with type 2 diabetes mellitus has a high diagnostic value and clinical application value.

scholarly journals Optimization of movement activity and the mental state of students by dance aerobics

2020 ◽  
Vol 3 (1) ◽  
pp. 121-131
Author(s):  
Natalia Chuprun ◽  
Igor Yurchenko
Keyword(s):  
Positive Influence ◽  
Female Students ◽  
Data Sets ◽  
Control Groups ◽  
Average Standard Deviation ◽  
Arithmetic Means ◽  
Arithmetic Average ◽  
Examination Time ◽  
State Of Rest ◽  
And Control

PURPOSE: to test the effectiveness of dance aerobics to optimize the motor activity and the psychophysical state of female students. METHODS: medical and biological: body weight (kg), BPM and BPD(mmHg), ChSS in a state of rest and the Stange test. All the data obtained by the study were processed by the procedures of descriptive and comparative statistical methods. From the area of the descriptive statistics the following parameters were defined: representative central and dispersive parameters: arithmetic average –; standard deviation –; initial and final measuring. Unpaired test, applied in comparative statistics, was performed in order to compare the arithmetic means of two independent data sets (experimental and control groups). Statistical analysis was performed by applying SPSS statistical software.RESULTS: During the studying, and especially the examination time, students experiencestrong psycho-emotional stress andthephysical state becomes worse. The use of dance aerobics has a positive influence on the psychophysical state of female students and the optimization of their physical activity.CONCLUSIONS:Comparative analysis of data confirmed the effectiveness of dance aerobics tools not only in the absence of negative changes during the examination session, but also improvement of the psychophysical state (state of health by the method of WAM in KG – 3,8 points, EG1 – 4,3 points, EG2 – 4,5 points, ЕG3 – 4.8 points) and the level of somatic health of students (in KG 0.23 ± 0.04 points, ЕG1 8.78 ± 0.50 points, ЕG2 8.77 ± 0.61 points, ЕG3 11, 65 ± 0.55 points)

scholarly journals The markers of stress in swine oral fluid

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marta Giergiel ◽  
Małgorzata Olejnik ◽  
Artur Jabłoński ◽  
Andrzej Posyniak
Keyword(s):  
Oral Fluid ◽  
Acute Stress ◽  
Statistical Significance ◽  
Alpha Amylase ◽  
Control Group ◽  
Protein Markers ◽  
Control Groups ◽  
Total Antioxidant ◽  
Acute Stress Response ◽  
And Control

Abstract Introduction The study measured the hormonal and protein markers of acute stress, those of oxidative stress and total antioxidant capacity (TAC) in swine oral fluid, determined which of these parameters would be the most appropriate for future livestock welfare assessment and established the time when the samples should be taken. Material and Methods Stress was induced in 7 out of 14 castrated six-week-old Danbred×Duroc pigs by immobilisation on a nasal snare at 8 a.m., 1 p.m., and 6 p.m. and samples were taken both directly after the stressor was applied and 30 min later. The remaining pigs were the control group, which were not immobilised; their samples were taken at the same times. The concentrations of hormones and malondialdehyde (MDA) were measured using liquid chromatography with tandem mass spectrometry, while those of alpha-amylase and TAC were measured using spectrophotometry. Results The levels of cortisol and cortisone increased with statistical significance immediately after the acute stress response and 30 min later. A cut-off value set at 0.25 ng/mL cortisol concentration was capable of distinguishing between the stressed and control groups with 100% accuracy in evening samples and 95% accuracy overall. Prednisolone was not present, and the levels of testosterone and corticosterone were low and not distinctive. Alpha-amylase became significantly more concentrated during stress induction and 30 min later. The TAC and MDA levels rose after the stress but without statistical significance. Conclusion The most suitable markers of acute stress were cortisol, cortisone and alpha-amylase. Oral fluid is a reliable material for monitoring the level of pigs’ stress and should be collected in the evening.

scholarly journals Die invloed van reuke op die werksatmosfeer van saalpersoneel binne 'n gespesialiseerde verpleegopset

1996 ◽  
Vol 22 (3) ◽  
Author(s):  
M. Jacobs ◽  
A. L. Barnard
Keyword(s):  
Healthcare Setting ◽  
Positive Influence ◽  
Test Method ◽  
Positive Contribution ◽  
Ward Staff ◽  
Control Groups ◽  
Two Samples ◽  
Work Atmosphere ◽  
Ward Personnel ◽  
And Control

The influence of odours on the work atmosphere of ward personnel within a specialised healthcare setting. The purpose of the investigation was to determine whether pleasant odours could make a positive contribution to the disposition of ward staff and their experience of the work ambience. A random test method was used to select two samples from a possible four wards. The sixteen men and women were allocated to experimental and control groups at random. The results indicate that odours could make a positive difference to the disposition of workers. No such positive influence was, however, observed with regard to the work ambience. Opsomming Die doel van die ondersoek was om vas te stel of aangename reuke die gemoedstoestand en belewenis van die werksatmosfeer van saalpersoneel positief kan beinvloed. Die lotingsmetode is gebruik om uit vier sale, twee ewekansige groepe te selekteer. Die sestien mans en vroue is ewekansig toegedeel aan 'n eksperimentele en kontrolegroep. Die resultate wys dat reuke wel 'n positiewe verskil aan die gemoedstoestand van 'n werker kan maak. Geen sodanige positiewe invloed is egter ten opsigte van werksatmosfeer waargeneem nie.

scholarly journals Influence of Synbiotics on Selected Oxidative Stress Parameters

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Paulina Kleniewska ◽  
Rafał Pawliczak
Keyword(s):  
Oxidative Stress ◽  
Lactobacillus Casei ◽  
Positive Influence ◽  
Control Groups ◽  
Oxidative Stress Parameters ◽  
Stress Markers ◽  
Sh Group ◽  
Synbiotic Supplementation ◽  
And Control ◽  
Stress Parameters

The aim of the present study was to assess synbiotic (Lactobacillus casei+ inulin) influence on oxidative stress parameters such as concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione, and free sulfhydryl groups content. Experiments were carried out on healthy volunteers (n=32). The subjects were divided into women group (n=16) and men group (n=16) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 wks, at the end of the study. The administration of synbiotic resulted in a significant decrease in MDA (p<0.01), H2O2(p<0.01), and GSSG concentrations (p<0.05) as compared with the control groups and significant increase in the concentrations of GSHt (p<0.001), GSH (p<0.01), and -SH group content (p<0.05) versus control. Synbiotics containingL. caseiplus inulin may have positive influence on selected oxidative stress markers.

Total Antioxidant Capacity of Saliva and its Relation with Early Childhood Caries and Rampant Caries

2009 ◽  
Vol 33 (3) ◽  
pp. 231-234 ◽  
Author(s):  
Amitha Hegde ◽  
Kavita Rai ◽  
Vivek Padmanabhan
Keyword(s):  
Oxidative Stress ◽  
Dental Caries ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Communicable Disease ◽  
Control Groups ◽  
Total Antioxidant ◽  
Unstimulated Saliva ◽  
And Control ◽  
Rampant Caries

Dental caries is an infectious and communicable disease and multiple factors influence the initiation and progression of the disease. Recently it has been claimed that oxidative stress may play an important role in the onset and the development of several inflammatory oral pathologies and dental caries may also be included. Saliva could constitute a first line of defense against free radical- mediated oxidative stress. This is the reason why antioxidant capacity of saliva has led to increasing interest and hence the need for this study. The antioxidant capacity of saliva was investigated in 100 children who were divided into four groups. Two of which comprised the study and control groups of children with ECC (below 71months of age) and the other two groups comprised of the study and control groups of the children with rampant caries [(RC) (6-12yrs)]. Unstimulated saliva samples were collected from all the groups. Total antioxidant capacity of saliva was evaluated by spectrophotometric assay. The results indicated that the total antioxidant capacity (TAC) of saliva increased in children with caries. TAC also increased with the age of the children.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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