scholarly journals Comparative evaluation of the antibacterial and cytotoxic activity of green synthesized and commercially available ZnO nanoparticles

Biomedicine ◽  
2021 ◽  
Vol 41 (3) ◽  
pp. 565-575
Author(s):  
Sushmit Mukherjee ◽  
Aparna Shil ◽  
Kunal Pal ◽  
Shinjini Pal ◽  
MausumiSikdar (ne’e Bhakta)
Keyword(s):  
Cytotoxic Activity ◽  
Comparative Evaluation ◽  
Zinc Oxide Nanoparticles ◽  
Mammalian Cells ◽  
Metallic Nanoparticles ◽  
Leaf Extract ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Antibacterial Potential

Introduction and Aim:Emergence of different applications of metallic nanoparticles in various field leads to innovation of new synthetic strategies. Besides being non-toxic to mammalian cells, zinc oxide nanoparticles (ZnONPs) has gained paramount attention due to its excellent antibacterial potential. This study illustrates a comparative analysis of antibacterial and cytotoxic activity of both phytochemically synthesized and chemically synthesized commercially available ZnONPs.   Materials and Methods:As a source of reducing agent, leaf extract of Coriander sativum was employed in case of green synthesis of ZnONPs. Several techniques, such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light Scattering (DLS) and Field emission Scanning Electron Microscopy (FESEM) were performed to characterize both green synthesized and commercial ZnONPs.Antibacterial potential of both theZnONPs were investigated on Gram-positive and Gram-negative bacterial strains to draw a correlative outcome. Hepatocellular cell line was used to determine the cytotoxic activity of both ZnONPs.   Results:Both the nanoparticles showed antibacterial and cytotoxic activity with measurable degree of difference.   Conclusion: From these studies it can be concluded, the green synthesized nanoparticles showed greater antibacterial as well as cytotoxic activity in comparison to the commercial ZnONPs.  

Bismuth(III) Thiophosphinates: Understanding How a Small Atomic Change Influences Antibacterial Activity and Mammalian Cell Viability

2020 ◽  
Vol 73 (12) ◽  
pp. 1226
Author(s):  
Dimuthu C. Senevirathna ◽  
Rebekah N. Duffin ◽  
Liam J. Stephens ◽  
Megan E. Herdman ◽  
Melissa V. Werrett ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Viability ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Gram Negative ◽  
X Ray ◽  
X Ray Crystallography ◽  
Biological Studies

Diphenylphosphinothioic acid (HSP(=O)Ph2) and diphenylphosphinodithioic acid (HSP(=S)Ph2) have been used to synthesise four BiIII complexes: 1 [Bi(SP(=O)Ph2)3], 2 [BiPh(SP(=O)Ph2)2], 3 [BiPh2(SP(=O)Ph2)], and 4 [Bi(SP(=S)Ph2)3], using BiPh3 and [Bi(OtBu)3] as bismuth sources. The complexes have been characterised by NMR spectroscopy, mass spectrometry, infrared spectroscopy, powder X-ray diffraction, and singe crystal X-ray crystallography (2–4). Biological studies indicated that despite complexes 2 and 3 reducing mammalian cell viability, their antibacterial activity provides a good degree of selectivity towards both Gram positive and Gram negative bacterial strains. The minimum inhibitory concentrations for complexes 2 and 3 are in the range of 0.52–5.5µM towards the bacteria tested. Homoleptic complexes 1 and 4 were generally less active towards both bacterial and mammalian cells.

Appraisal of innovative phytosynthetic CdO NPs from leaf extract of Ocimum Sanctum and their bactericidal activity against different strains

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Aarth R ◽  
Sudha A P ◽  
Sujatha B ◽  
Sowmya Lakshmi K
Keyword(s):  
Leaf Extract ◽  
Cadmium Oxide ◽  
Morphological Properties ◽  
Precipitation Method ◽  
Ocimum Sanctum ◽  
Oxide Nanoparticles ◽  
Average Crystalline Size ◽  
Face Centered Cubic ◽  
Bacterial Strains ◽  
X Ray

The phytosynthesis of n-type Cadmium Oxide Nanoparticles reduces the toxicity of the substance and makes it Eco-friendly. This Eco-friendly biosynthesis of CdO NPs was synthesized for the first time from the Queen of herbs, Ocimum Sanctum (holy basil).The biosynthesized Cadmium oxide was prepared using Ocimum leaf extract as a reductant and Cadmium Chloride and hydroxide as cadmium and oxide source materials by Co- Precipitation method. Thus obtained Cadmium Oxide Nanoparticles were characterized by different techniques such as X-ray diffraction (XRD), Fourier Transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM),Energy dispersive X-ray spectroscopy(EDS) to study the structural and morphological properties. XRD pattern exhibited the formation of face centered cubic structure of CdO NPs with an average crystalline size of 11.5nm .The chemical bond formation of CdO NPs were confirmed by FTIR spectrum in the range of (400-4000cm-1). The SEM micrographs revealed the predominant formation of Cauliflower shape with a particle size in the range of 61-142nm. The high purity of the biosynthesized nanoparticles were confirmed by EDS analysis. Further it was tested against gram positive and gram negative bacterial strains and showed significant antibacterial activity. This biosynthetic research study opens an innovative window to progress our understanding of how CdO NPs shows resistance to different bacterial strains.

scholarly journals Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Ethanolic Extract ◽  
Spherical Shape ◽  
Bacterial Strains ◽  
X Ray ◽  
Coccinia Indica ◽  
Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

Selenylation, Characterization and Cytotoxic Activity of Seleno-Astragalus Polysaccharide

2013 ◽  
Vol 411-414 ◽  
pp. 3146-3149 ◽  
Author(s):  
Yu Bin Ji ◽  
Fang Dong ◽  
Miao Yu
Keyword(s):  
Cytotoxic Activity ◽  
Human Breast Cancer ◽  
Human Breast ◽  
X Ray Diffraction ◽  
X Ray ◽  
Astragalus Polysaccharide ◽  
Series Of Experiments ◽  
Dose Dependent ◽  
Mcf 7

This paper investigated the selenylation, characterization and cytotoxic activity of seleno-Astragalus polysaccharide. Firstly, Se-APS was synthesized under reaction time at 8 h, reaction temperature at 80 °C, ratio of Na2SeO3 to APS at 1.0 g/g and water bath shaking rate at 40 r/min. Then, a series of experiments were designed to investigate the characterization and cytotoxicity of Se-APS. The result indicated that the characterization of Se-APS was significantly different from APS, except for X-ray diffraction. Additionally, MTT assay conformed that Se-APS could significantly inhibit the proliferation of human breast cancer MCF-7 cells in dose-dependent and time-dependent manners.

scholarly journals Controlled Release of Ropivacaine from Single-Armed (1-PCL) and Four-Armed PCL (4-PCL) Microspheres

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Wei Xiong ◽  
Yue-kun Shen ◽  
Peng Dong ◽  
Ying Xiao ◽  
Xiong-qing Huang ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Cell Proliferation ◽  
Mammalian Cells ◽  
Great Promise ◽  
X Ray Diffraction ◽  
X Ray ◽  
Initial Release ◽  
Release Assay ◽  
Ft Ir

Sustained release of anesthesia has shown great promise in the treatment of chronic pain in patients. In this research, we used neutralized ropivacaine as an anesthesia and poly(ε-caprolactone) (PCL) with different architectures to systematically study how these architectures affect the release of ropivacaine. After optimizing the parameters of the preparation of microspheres, ropivacaine-loaded 1-PCL microspheres and 4-PCL microspheres were obtained. Fourier Transform infrared spectra (FT-IR) and X-ray diffraction spectra (XRD) confirmed that ropivacaine was encapsulated within the microsphere rather than inserted on the surface of the microsphere. Ropivacaine was found to be buried deeper in the 1-PCL microsphere than in the 4-PCL microsphere. In vitro release assay revealed that small crystalline grains interfered with ropivacaine release in 4-PCL microspheres during the initial release period, but then two kinds of microspheres showed a similar ropivacaine release rate. We basically proved that the architecture of PCL has a negligible effect on ropivacaine release. Cell proliferation test revealed that the release of products from the microspheres resulted in insignificant toxicity towards mammalian cells.

scholarly journals Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 38 ◽  
Author(s):  
Banzeer Ahsan Abbasi ◽  
Javed Iqbal ◽  
Riaz Ahmad ◽  
Layiq Zia ◽  
Sobia Kanwal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Energy Dispersive ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
X Ray ◽  
Transmission Electron

This study attempts to obtain and test the bioactivities of leaf extracts from a medicinal plant, Geranium wallichianum (GW), when conjugated with zinc oxide nanoparticles (ZnONPs). The integrity of leaf extract-conjugated ZnONPs (GW-ZnONPs) was confirmed using various techniques, including Ultraviolet–visible spectroscopy, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, energy-dispersive spectra (EDS), scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The size of ZnONPs was approximately 18 nm, which was determined by TEM analysis. Additionally, the energy-dispersive spectra (EDS) revealed that NPs have zinc in its pure form. Bioactivities of GW-ZnONPs including antimicrobial potentials, cytotoxicity, antioxidative capacities, inhibition potentials against α-amylase, and protein kinases, as well as biocompatibility were intensively tested and confirmed. Altogether, the results revealed that GW-ZnONPs are non-toxic, biocompatible, and have considerable potential in biological applications.

scholarly journals Nanangenines: drimane sesquiterpenoids as the dominant metabolite cohort of a novel Australian fungus, Aspergillus nanangensis

2019 ◽  
Vol 15 ◽  
pp. 2631-2643 ◽  
Author(s):  
Heather J Lacey ◽  
Cameron L M Gilchrist ◽  
Andrew Crombie ◽  
John A Kalaitzis ◽  
Daniel Vuong ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biosynthetic Pathway ◽  
Spectroscopic Analysis ◽  
Bioinformatics Analysis ◽  
Biosynthetic Gene Cluster ◽  
In Vitro Activity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Drimane Sesquiterpenoids

Chemical investigation of an undescribed Australian fungus, Aspergillus nanangensis, led to the identification of the nanangenines – a family of seven new and three previously reported drimane sesquiterpenoids. The structures of the nanangenines were elucidated by detailed spectroscopic analysis supported by single crystal X-ray diffraction studies. The compounds were assayed for in vitro activity against bacteria, fungi, mammalian cells and plants. Bioinformatics analysis, including comparative analysis with other acyl drimenol-producing Aspergilli, led to the identification of a putative nanangenine biosynthetic gene cluster that corresponds to the proposed biosynthetic pathway for nanangenines.

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