scholarly journals EVALUATION OF THE USE OF DIFFERENT SOLVENTS FOR PHYTOCHEMICAL CONSTITUENTS AND ANTIOXIDANTS ACTIVITY OF THE LEAVES OF MURRAYA KOENIGII (LINN.) SPRENG. (RUTACEAE)

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Aisha Idris Ali ◽  
Virginia Paul ◽  
Amit Chattree ◽  
Ranu Prasad ◽  
Ajit Paul ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Chemical Components ◽  
Murraya Koenigii ◽  
Antioxidants Activity ◽  
Wide Range ◽  
Extraction Solvents ◽  
Phytochemical Constituents ◽  
The Impact ◽  
Promising Source

Murraya koenigii (Rutaceae) is a promising source of bioactive compounds since the leaves of this plant has been traditionally used extensively in the Indian Ayurvedic system of medicine for the treatment of a wide range of diseases and disorders. Although the pharmacological effect of the plant’s bioactive compounds has been extensively studied, however, study on the effect of using different extraction solvents to extract these bioactive componentsis scarce. The aim of the present study was to evaluate the impact of different solvents on extraction yields, phytochemical constituents and antioxidants activity of dehydrated Murrayakoenigi leaves. The results showed that the used solvents play an important role in the yield of extraction and the content of chemical components. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (5.70%) as well as the highest content of phenolic (27.2 mg GAE/g DW) and flavonoid (15.55 mg QE/g DW). The extract obtained from methanol exhibited highest antioxidant scavenging activity (93%), (using 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging assay), and the antioxidant activity of Murraya koenigi leaves extract was found to be higher than ascorbic acid. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants constituents from Murraya koenigi leaves for utilization in pharmacognosy. To best of our knowledge this is the first report that directly compares these 4 extraction solvents for the extraction of bioactive components from Murraya koenigi leaves.

scholarly journals Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Dieu-Hien Truong ◽  
Dinh Hieu Nguyen ◽  
Nhat Thuy Anh Ta ◽  
Anh Vo Bui ◽  
Tuong Ha Do ◽  
...  
Keyword(s):  
Biological Activities ◽  
High Capacity ◽  
Inflammatory Activity ◽  
Chemical Components ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
The Impact ◽  
Promising Source ◽  
Severinia Buxifolia

Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50 value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.

Preliminary phytochemical analysis and evaluation of antioxidant, cytotoxic and inhibition of lipopolysaccaride - induced NOS (iNOS) expression in BALB/c mice liver by Ziziphus oenoplia Mill. fruit

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Ramaraj Thirugnanasampandan ◽  
Gunasekar Ramya ◽  
Gunasekaran Bhuvaneswari ◽  
Srinivasan Aravindh ◽  
Suresh Vaishnavi ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Wide Range ◽  
Mice Liver

AbstractBackground:Mill. is an ethnomedicinal plant and its fruit has been traditionally used by Puliar tribes of Anamalai Hills, Tamil Nadu, India to treat various ailments. Phytochemical analysis, antioxidant, cytotoxic and inducible nitric oxide synthase (Methods:To explore bioactive compounds present in the ripened fruits, high-performance thin-layer chromatography (HPTLC) and gas chromatography/mass spectrometry (GC–MS) analysis were done. Free radical scavenging, hepatoprotective, inhibition ofResults:Total flavonoid content of ZOFwas estimated as 69 µg/mg catechin equivalent. HPTLC densitogram confirmed the presence of quercetin and GC–MS analysis showed a total of 16 compounds of 87.66 % with quinic acid as a major compound which accounted for 22.29 %. Free radical-scavenging activity of ethanolic fruit extract was ranged from 160.12 to 650.23 µg/mL. An amount of 1.5 µg lipopolysaccharide (LPS)- induced severe inflammation in BALB/c mice liver, followed by treatment with ethanolic fruit extract of 100 µg concentration, exhibited significant hepatoprotection and reverse transcriptase polymerase (RT-PCR) analysis showed downregulation ofConclusions:Findings of the present study prove that ZOF is a rich source of bioactive compounds with a wide range of pharmacological activities. Hence, consumption of this wild edible fruit will be a cost-effective and easily available natural nutritional source for health protection.

scholarly journals Phytochemical Constituents Propolis Flavonoid, Immunological Enhancement, Anti-porcine Parvovirus Activities of isolated from the Propolis

2020 ◽  
Author(s):  
Xia Ma ◽  
zhenhuan guo ◽  
zhiqiang zhang ◽  
xianghui li ◽  
yizhou lv ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Underlying Mechanism ◽  
Chemical Components ◽  
Porcine Parvovirus ◽  
Wide Range ◽  
Inhibition Antibody ◽  
Antibody Titers ◽  
Phytochemical Constituents ◽  
Immune Enhancement ◽  
Negative Ionization

Propolis was widely used in health preservation and disease healing, it contains many ingredients. The previous study had been revealed that the propolis has a wide range of efficacy, such as antiviral, immune enhancement, anti-inflammatory and so on, but its antiviral components and underlying mechanism of action remain unknown. In this study, we investigated the chemical composition, and anti-PPV and immunological enhancement of Propolis Flavonoid(PF). Chemical composition of PF was distinguished by UPLC-Q/TOF-MS/MS analysis.The presence and characterized of 26 major components was distinguished in negative ionization modes.To evaluate the effects of PF used as adjuvant on the immune response porcine parvovirus (PPV). Thirty Landrace-Yorkshire hybrid sows were randomly assigned to 3 groups, and the sows in adjuvant groups were intramuscular injected PPV vaccine with 2.0 mL PF adjuvant (PA), oilemulsion adjuvant (OA), respectively. After that, serum hemagglutination inhibition antibody titers, IgM and IgG subclasses, eripheral lymphocyte proliferation activity, and concentrations of cytokines were measured. Results indicated an enhancing effect of PA on IgM, IL-2, IL-4, IFN-γ and the IgG subclass responses. These findings suggested that PA could significantly enhance the immune responses. Furthermore, we screened the chemical components the effective of anti-PPV, Ferulic acid have an excellently anti-PPV effective.

scholarly journals Evaluation of the chemical components and some bioactive compounds of the peppermint essential oil (Mentha arvensis L.) cultivated in Vietnam

2021 ◽  
Vol 63 (7) ◽  
pp. 26-30
Author(s):  
Bao Tram Tran ◽  
◽  
Thi Hien Nguyen ◽  
Thi Chien Truong ◽  
Xuan Binh Minh Phan ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Raw Material ◽  
Chemical Components ◽  
Plant Resources ◽  
Scavenging Capacity ◽  
Research Endeavor ◽  
Diverse Plant

Vietnam has diverse plant resources and many species are considered to be valuable medicinal sources. Studying, seeking, and evaluating the bioactive compounds from plants is a valuable research endeavor for producing human healthcare products. Peppermint (Mentha arvensisL.) is widely cultivated in Vietnam as a raw material for the production of essential oils serving local needs and exports. This study compared the chemical components and some bioactive compounds in essential oil from Mentha arvensisL. cultivated in Thai Binh, Binh Thuan, and An Giang provinces. The essential oil yields in all samples ranged from 0.69 to 0.84%. Gas chromatography analyses identified 29 substances in total. Menthol and menthone were the two main ingredients in the oils, accounting for 53.62-62.61% and 18.81-21.06%, respectively. Furthermore, all essential oils exhibited free radical scavenging capacity in vitroand antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli. The plants collected in Thai Binh had the highest content of essential oil, menthol, and menthone, as well as the strongest bioactivities.

scholarly journals Effect of extraction solvents on antioxidant and wound healing properties of Carica papaya leaves extract

Food Research ◽  
2020 ◽  
Vol 4 (S2) ◽  
pp. 76-83
Author(s):  
H.H. Soib ◽  
H.F. Ismail ◽  
H. Yaakob ◽  
M.K.H. Idris ◽  
A. Abd Aziz
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Bioactive Compounds ◽  
Carica Papaya ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Skin Fibroblast ◽  
Migration Assay ◽  
Extraction Solvents

Carica papaya is a well-known plant that has been reported to exert various pharmacological activities including antioxidant and wound healing properties. However, to date, the lack of scientific evidence has been explored on the efficiency of the solvents towards C. papaya extract as a potential wound healer. The selection of proper extraction solvent plays a pivotal role in extracting the bioactive compounds from the plant. Therefore, the present study was aimed to examine the effect of three types of extraction solvents (methanol, ethanol and aqueous) on the antioxidant activity and wound healing potential of C. papaya leaves. In this study, the effect of different solvents of C. papaya leaves extracts were determined through 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, cytotoxicity assay and scratch migration assay on Human Skin Fibroblast cells (HSF1184). The result showed that the methanolic extract of C. papaya possessed a significant antioxidant activity as compared to ethanol and aqueous extract. The result also demonstrated that methanolic extract significantly stimulates the highest migration rate of HSF1184 cell at a concentration of 3.9 µg/mL, 7.8 µg/mL and 15.6 µg/ mL (p  0.05) after 48 hrs with no cytotoxicity observed at the concentration lower than 1000 mg/mL as compared to other solvents. HPLC analysis of methanol extract identified the presence of two flavonoids (catechin, quercetin) and two phenolic acids (caffeic acid, cinnamic acid). The findings suggest that the methanolic extract of C. papaya is effective in fighting free radicals and it has good wound healing activities. It also has the potential to be further explored for its medicinal values owing to the abundance of bioactive compounds from the extract.

scholarly journals Significance and potential of marine microbial natural bioactive compounds against biofilms/biofouling: necessity for green chemistry

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5049 ◽  
Author(s):  
Mohd Adnan ◽  
Eyad Alshammari ◽  
Mitesh Patel ◽  
Syed Amir Ashraf ◽  
Saif Khan ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Sea Water ◽  
Cell Communication ◽  
Life Forms ◽  
Bioactive Metabolites ◽  
Chemical Components ◽  
Toxic Substances ◽  
Marine Microbes ◽  
Terrestrial Environments ◽  
Promising Source

Natural products from the unique environments of sea water and oceans represent a largely unfamiliar source for isolation of new microbes, which are potent producers of secondary bioactive metabolites. These unique life-forms from the marine ecosphere have served as an important source of drugs since ancient times and still offer a valuable resource for novel findings by providing remedial treatments. Therefore, it can be expected that many naturally bioactive marine microbial compounds with novel structures and bioactivities against those from terrestrial environments may be found among marine metabolites. Biofilms in aquatic environment possess serious problems to naval forces and oceanic industries around the globe. Current anti-biofilm or anti-biofouling technology is based on the use of toxic substances that can be harmful to their surrounding natural locales. Comprehensive research has been done to examine the bioactive potential of marine microbes. Results are remarkably varied and dynamic, but there is an urgent need for bioactive compounds with environmentally friendly or “green” chemical activities. Marine microbes have the potential as upcoming and promising source of non-toxic compounds with sustainable anti-biofouling/anti-biofilm properties as they can produce substances that can inhibit not only the chemical components required for biofilm production but also the attachment, microorganism growth, and/or cell–cell communication.

Interactions of Clethodim and Sethoxydim with Other Pesticides

2013 ◽  
Vol 40 (2) ◽  
pp. 127-134
Author(s):  
Gurinderbir S. Chahal ◽  
David L. Jordan ◽  
Alan C. York ◽  
Rick L. Brandenburg ◽  
Barbara B. Shew ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Adverse Impact ◽  
Chemical Components ◽  
Biotic And Abiotic Stresses ◽  
Solution Ph ◽  
Wide Range ◽  
Annual Grasses ◽  
The Impact ◽  
Large Crabgrass

ABSTRACT A wide range of agrochemicals are applied in peanut to manage biotic and abiotic stresses. Experiments were conducted to evaluate the efficacy of clethodim and sethoxydim applied alone or with dimethenamid-P, pyraclostrobin, S-metolachlor, and 2,4-DB. When applied in combination with clethodim, pyraclostrobin often reduced goosegrass and large crabgrass control compared with clethodim only. Pyraclostrobin did not impact efficacy of sethoxydim in most instances. Dimethenamid-P and S-metolachlor did not negatively affect efficacy of clethodim and sethoxydim. The impact of 2,4-DB on efficacy of clethodim and sethoxydim was inconsistent. The magnitude of adverse impact on graminicides (clethodim and sethoxydim) performance did not exceed 19% and was caused primarily by pyraclostrobin. Conversely, increased efficacy of graminicides caused by chloroacetamide herbicides did not exceed 14%. Graminicides and chloroacetamide herbicides changed solution pH from slightly acidic to highly acidic. Several combinations of clethodim and sethoxydim produced temporary precipitates but no permanent precipitates. Results from these experiments suggest that applying tank mixtures containing up to four chemical components will not dramatically reduce control of emerged annual grasses in peanut.

scholarly journals Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L.

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Thanh Van Ngo ◽  
Christopher James Scarlett ◽  
Michael Christian Bowyer ◽  
Phuong Duc Ngo ◽  
Quan Van Vuong
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Organic Solvents ◽  
Bioactive Compounds ◽  
Total Solid ◽  
Extraction Solvent ◽  
Phytochemical Composition ◽  
Extraction Solvents ◽  
The Impact

This study aimed to study the impact of selected common organic solvents on extractable solids, phytochemical composition, and antioxidant capacity of S. chinensis. The results showed that the tested solvents played an important role in extraction of total solid and phytochemical composition as well as antioxidant capacity of S. chinensis. Acetone (50% v/v) was found to be the optimal extraction solvent for extractable solids (12.2%), phenolic compounds (60 mg GAE/g DW), flavonoids (100 mg CE/g DW), proanthocyanidins (47.4 mg CE/g DW), and saponins (754 mg EE/g DW) as well as antioxidant capacity (ABTS 334 mM TE/g DW, DPPH 470 mM TE/g DW, FRAP 347 mM TE/g DW, and CUPRAC 310 mM TE/g DW). The extract prepared from 50% acetone had high levels of bioactive compounds (TPC 555 mg GAE/g CRE, flavonoids 819 mg CE/g CRE, proanthocyanidins 392 mg CE/g CRE, and saponins 1,880 mg EE/g CRE) as well as antioxidant capacity (ABTS 414 mM TE/g, DPPH 407 mM TE/g, FRAP 320 mg TE/g, and CUPRAC 623 mM TE/g), thus further confirming that 50% acetone is the solvent of choice. Therefore, 50% acetone is recommended for extraction of phenolic compounds, their secondary metabolites, saponins, and antioxidant capacity from the root of S. chinensis for further isolation and utilisation.

scholarly journals Effect of Two Drying Methods on the Bioactive Cashew Apple Varieties Consumed in the City of Garoua (Northern Cameroon)

2021 ◽  
pp. 64-77
Author(s):  
Kouogueu Seuyim Ghislain ◽  
Nguedjo Wandji Maxwell ◽  
Dibacto Kemadjou Ruth Edwige ◽  
Nseme Mboma Yves Didier ◽  
Djouka Nembot Pelagie Marcel ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Drying Methods ◽  
Dpph Radical ◽  
Constant Weight ◽  
Antioxidant Power ◽  
Cashew Apple ◽  
The Impact

Aims: The cashew tree (family Anacardiaceae) grows widely in many parts of African countries, including Cameroon. Its fruit and nut are used for food and several studies have shown their beneficial effects on health. This work aimed to evaluate the impact of two drying methods on the content of bioactive compounds and antioxidant activity. Methodology: Four varieties (VAR 1, VAR 2, VAR 3, VAR 4) of cashew apple samples were collected and drying using sun-dried and oven-dried to a constant weight, and then ground in a blender to a powder, the fresh one was cut up and crushed in a blender. All sample were reconstituted with distilled water and polyphenols, flavonoids, alkaloids contents, and antioxidant activity through different mechanisms (DPPH radical, FRAP and TAC assays) were assessed. Results: Alkaloids ranged from 1.50 mg EQui/g MF to 5.69 mg EQui/g DM for fresh and oven-dried VAR 1 respectively, polyphenols ranged from 786.15 mg EAG/g MF to 2836.92 mg EAG/g DM for fresh and oven-dried VAR 1 respectively, flavonoids ranged from 8.18 mg EAG/g MF to 295.45 mg EAG/g DM for fresh and oven-dried VAR 2 respectively. TAC values ranged from 13.09 mg EAA/g MF to 67.06 mg EAA/g for fresh and oven-dried VAR3 and VAR2 respectively. The highest DPPH radical scavenging value (86.25%) was obtained with fresh VAR 4 and the lowest (25.67%) with fresh VAR 1. The highest ferric reducing antioxidant power (FRAP) was obtained with fresh VAR 1 and VAR 3 (0.27 mg AAE/g MF) and the lowest with VAR 3 and VAR 4 oven-dried (0.23 mg AAE/g MF). Conclusion: In conclusion, the different cashew varieties studied in this work are a good source of antioxidants. The drying method significantly affects bioactive compounds and antioxidant activities. A weak but not significant correlation was obtained between the number of bioactive compounds and antioxidant activities.

scholarly journals Evaluation of phytochemical constituents and antioxidant potential of hydro-alcoholic and aqueous extracts of Murraya koenigii L. and Ficus carica L.

2019 ◽  
Vol 65 (4) ◽  
pp. 7-17
Author(s):  
Shah Asma Farooq ◽  
Randhir Singh ◽  
Vipin Saini
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Ficus Carica ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Murraya Koenigii ◽  
Dried Fruits ◽  
Phytochemical Constituents

SummaryIntroduction:Murraya koenigii L. and Ficus carica L. have been traditionally used in folk medicine for treating several diseases.Objective: The present study was proposed to investigate the phytochemical constituents and antioxidant potential of hydro-alcoholic and aqueous extracts of leaves of M. koenigii and dried fruits of F. carica.Methods: Phytochemical screening was performed using different methods and antioxidant activity was evaluated by measuring total phenolic content, total antioxidant capacity (TAOC), hydrogen peroxide (H2O2) scavenging activity, DPPH radical-scavenging activity, reducing power assay, nitric oxide radical scavenging activity, and superoxide radical-scavenging activity.Results: The results showed that the hydro-alcoholic and aqueous extracts of leaves of M. koenigii and dried fruits of F. carica possess a significant quantity of flavonoids, saponins, terpenoids (9.0%, 0.9%, 0.6% and 7.3%, 8.7%, 0.44%, respectively) and also possess a significant antioxidant activity as evaluated by employing different antioxidant assays.Conclusion: It may be concluded that the hydro-alcoholic and aqueous extracts of leaves of M. koenigii and dried fruits of F. carica exhibit significant antioxidant activity.

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