ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

Abstrak Kandungan daun kelor (Moringa oleifera L) diketahui dapat berkhasiat sebagai antijamur. Penelitian ini bertujuan untuk memformulasi dan mengetahui aktivitas antijamur gel yang mengandung daun kelor terhadap jamur Malasezia furfur. Ekstraksi daun kelor dilakukan menggunakan etanol 70% dengan metode maserasi. Formulasi gel yang mengandung ekstrak etanol daun kelor dilakukan menggunakan hidroksi propil metil selulosa (HPMC) dengan berbagai konsentrasi, yaitu 2, 3, dan 4%., Evaluasi gel yang dilakukan adalah uji organoleptik, homogenitas, pH, daya sebar, daya lekat, dan viskositas. Kemudian dilakukan uji aktivitas antijamur dengan menggunakan metode difusi padat dengan kertas cakram, dan digunakan gel ketokonazol sebagai pembanding. Parameter yang diukur adalah zona hambat antijamur. Hasil kemudian dianalisis statistik Kruskall Wallis dan Mann Withney. Berdasarkan evaluasi gel, diperoleh bahwa peningkatan konsentrasi basis HPMC 2, 3, dan 4% dalam sediaan gel ekstrak etanol daun kelor dapat meningkatkan viskositas gel, daya lekat gel, menyebabkan penurunan daya sebar gel, dan mempengaruhi peningkatan konsistensi sediaan gelpada uji organoleptik tanpa mempengaruhi uji pH gel dan homogenitas gel. Formula yang memiliki sifat fisik gel yang yang baik jika dilihat dari hasil uji sifat fisik gel yang meliputi uji organoleptik,homogenitas, pH, daya sebar daya lekat dan viskositas yaitu formula gel ekstrak etanol daun kelor dengan konsentrasi basis HPMC 2 % karena hampir dari semua uji memenuhi persyaratan literatur. Dari penelitian ini dapat disimpulkan gel ekstrak etanol daun kelor mempunyai aktivitas antijamur M.furfur dan gel yang mengandung HPMC 2% memiliki sifat fisik gel dan aktivitas antijamur lebih baik jika dibandingkan dengan gel konsentrasi HPMC 3 dan 4%. Kata Kunci: Daun Kelor, Moringa oleifera L, gel, antijamur, Malassezia furfur. The effectiveness test of gel of ethanol extract of Moringa oleifera leaves as antifungal of Malassezia furfur Abstract Moringa leaf content (Moringa oleifera L) is known to be efficacious as an antifungal. The aim of this research is to formulate and to know gel antifungal activity containing moringa leaf against Malasezia furfur fungus. Moringa leaf extraction was done using 70% ethanol by maceration method. The gel formulation containing ethanol extract of moringa was conducted using hydroxy propyl methyl cellulose (HPMC) with various concentrations, ie 2, 3, and 4%. Evaluation of gel performed was organoleptic, homogeneity, pH, spreading, and viscosity. Then the antifungal activity was tested using solid diffusion method with paper disc, and used ketoconazole gel as comparison. The measured parameter was the antifungal block zone. The results were then analyzed statistically using Kruskall Wallis and Mann Withney. Based on the gel evaluation, it was found that the increased concentrations of HPMC 2, 3, and 4% base concentrations in the gel preparation of ethanol extract of moringa leaf can increase gel viscosity, gel adhesiveness, decrease the gel power, and influence the consistency of preparation for organoleptic test without affecting the test pH gel and gel homogeneity. Formula which have good physical properties of gel when viewed from the test results of physical properties of gel which include organoleptic test, homogeneity, pH, viscosity sticky power and viscosity is gel gel ethanol extract leaf kelor with 2% HPMC base concentration because almost of all test meet the literature requirements. From this study, it can be concluded that leaf extract gel ethanol gel has antifungal activity of M.furfur and gel containing HPMC 2% has gel physical properties and antifungal activity is better when compared with HPMC 3 and 4% concentration gel Keywords: Moringa olifera L.,gel, antifungal, Malasezia furfur

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ANTI-FUNGAL ACTIVITY OF ACALYPHA WILKESIANA: A PRELIMINARY STUDY OF FUNGAL ISOLATES OF CLINICAL SIGNIFICANCE

African Journal of Infectious Diseases ◽

10.21010/ajid.v16i1.4 ◽

2021 ◽

Vol 16 (1) ◽

pp. 21-30

Author(s):

Katibi Oludolapo Sherifat ◽

Aboh Mercy Itohan ◽

Salawu Oluwakayinsola Adeola ◽

Kola -Mustapha Adeola ◽

Olatunji Lawrence Aderemi

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Trichophyton Rubrum ◽

Seborrheic Dermatitis ◽

Ethanol Extract ◽

Microsporum Canis ◽

Plant Materials ◽

Acalypha Wilkesiana ◽

Anti Fungal

Acalypha wilkesiana (AW), a popular medicinal plant has been used in traditional medicine to treat a variety of skin disorders including pityriasis versicolor and seborrheic dermatitis. As a prelude to clinical trials in humans, an experimental study was carried out to determine the spectrum of antifungal activity of 2 variants of the Acalypha wilkesiana plant. Materials and Methods: The ethanol extract and herbal cream formulation of the dried leaves of 2 cultivars (Macrophylla & Hoffmani) of Acalypha wilkesiana were investigated for in-vitro antifungal activity by disc diffusion and micro-broth dilution techniques. Organisms tested were typed cultures of Malassezia furfur, Candida albicans and Trichophyton rubrum; and clinical strains of Microsporum canis and Epidermophyton floccosum. Results: Both cultivars (Macrophylla and Hoffmanii) of the plant showed good activity against all the fungi tested except Microsporum canis (8.0±0.00; 7.00±0.00 mm). The greatest activity was observed against Trichophyton rubrum (22.0±0.00; 24.00±0.00 mm) while Candida albicans showed the least activity (15.0±0.00; 18.00±0.57 mm). The Minimum Inhibitory Concentration (MIC) of the crude extract ranged between 0.25 and 8 mg/ml for all organisms, while that of the herbal cream was 0.31-8mg/ml. The lowest MIC was seen with Candida albicans for both varieties of the plant. The Acalypha wilkesiana Hoffmanii demonstrated a greater activity against Candida albicans and Malassezia furufur than the A. wilkesiana (Macrophylla). Conclusion: This study reveals Acalypha wilkesiana leaf extract has potential for development as a cream that can be used to treat superficial fungal skin infections.

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Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans

WARTA FARMASI ◽

10.46356/wfarmasi.v8i2.117 ◽

2019 ◽

Vol 8 (2) ◽

pp. 71-80

Author(s):

Ummu Kalsum ◽

Ayu Ayu

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Daucus Carota ◽

Ethanol Extract ◽

Diffusion Method ◽

Daucus Carota L ◽

Zone Diameter ◽

Extract Concentration ◽

Activity Test ◽

Agar Diffusion Method

ABSTRAK Telah dilakukan penelitian Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol umbi wortel sebagai antifungi terhadap pertumbuhan Candida albicans dengan menggunakan variasi konsentrasi yang berbeda yaitu 1% b/v, 3% b/v dan 5% b/v. Ekstrak etanol umbi wortel diperoleh dengan cara maserasi menggunakan pelarut etanol 70%. Pengujian aktivitas antifungi menggunakan metode difusi agar dengan cara sumuran untuk mengetahui aktivitas antifungi dengan mengamati daerah hambatan. Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak 1% b/v, 3% b/v dan 5% b/v dapat memberikan aktivitas yang menghambat pertumbuhan jamur uji. Terdapat penambahan diameter zona hambat pada setiap kenaikan konsentrasi yaitu 1% b/v (15,7 mm), 3% b/v (18,9 mm), dan 5% b/v (19,8 mm) terhadap jamur Candida albicans. Semua variasi konsentrasi ekstrak memiliki aktivitas antifungi yang kuat dalam menghambat pertumbuhan Candida albicans. Kata kunci: Ekstrak, Daucus carota L., Antifungi, Candida albicans ABSTRACT It has conducted a study of activity test of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans. This the study aims at knowing the activity of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans by using different variance concentration they are 1% b/v, 3% b/v and 5% b/v. Carrot tuber ethanol the extract was obtained by maceration by using 70% of ethanol dissolver. Activity test of antifungal by using agar diffusion method with a well method to find out the the activity of antifungal by observing the obstruction zone. The result of this research shows that concentration extract 1% b/v, 3% b/v and 5% b/v can give activity which impedes the growth of fungal test. There is increasing of obstruction zone diameter in every increment of of the concentration they are 1% b/v (15,7 mm), 3% b/v (18,9 mm) and 5% b/v (19,8mm) toward Candida albicans fungi. All extract concentration variation has a strong antifungal activity in impeding the growth of Candida albicans.

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Uji efektivitas gel ekstrak etanol daun kelor (Moringa oleifera L.) sebagai antijamur Malassezia furfur

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.119 ◽

2017 ◽

Vol 5 (2) ◽

pp. 62

Author(s):

Anna L Yusuf ◽

Ecin Nurawaliah ◽

Nurhidayati Harun

Keyword(s):

Antifungal Activity ◽

Physical Properties ◽

Moringa Oleifera ◽

Methyl Cellulose ◽

Ethanol Extract ◽

Diffusion Method ◽

Measured Parameter ◽

Malassezia Furfur ◽

Organoleptic Test ◽

Solid Diffusion

Abstrak Kandungan daun kelor (Moringa oleifera L) diketahui dapat berkhasiat sebagai antijamur. Penelitian ini bertujuan untuk memformulasi dan mengetahui aktivitas antijamur gel yang mengandung daun kelor terhadap jamur Malasezia furfur. Ekstraksi daun kelor dilakukan menggunakan etanol 70% dengan metode maserasi. Formulasi gel yang mengandung ekstrak etanol daun kelor dilakukan menggunakan hidroksi propil metil selulosa (HPMC) dengan berbagai konsentrasi, yaitu 2, 3, dan 4%., Evaluasi gel yang dilakukan adalah uji organoleptik, homogenitas, pH, daya sebar, daya lekat, dan viskositas. Kemudian dilakukan uji aktivitas antijamur dengan menggunakan metode difusi padat dengan kertas cakram, dan digunakan gel ketokonazol sebagai pembanding. Parameter yang diukur adalah zona hambat antijamur. Hasil kemudian dianalisis statistik Kruskall Wallis dan Mann Withney. Berdasarkan evaluasi gel, diperoleh bahwa peningkatan konsentrasi basis HPMC 2, 3, dan 4% dalam sediaan gel ekstrak etanol daun kelor dapat meningkatkan viskositas gel, daya lekat gel, menyebabkan penurunan daya sebar gel, dan mempengaruhi peningkatan konsistensi sediaan gelpada uji organoleptik tanpa mempengaruhi uji pH gel dan homogenitas gel. Formula yang memiliki sifat fisik gel yang yang baik jika dilihat dari hasil uji sifat fisik gel yang meliputi uji organoleptik,homogenitas, pH, daya sebar daya lekat dan viskositas yaitu formula gel ekstrak etanol daun kelor dengan konsentrasi basis HPMC 2 % karena hampir dari semua uji memenuhi persyaratan literatur. Dari penelitian ini dapat disimpulkan gel ekstrak etanol daun kelor mempunyai aktivitas antijamur M.furfur dan gel yang mengandung HPMC 2% memiliki sifat fisik gel dan aktivitas antijamur lebih baik jika dibandingkan dengan gel konsentrasi HPMC 3 dan 4%. Kata Kunci: Daun Kelor, Moringa oleifera L, gel, antijamur, Malassezia furfur. The effectiveness test of gel of ethanol extract of Moringa oleifera leaves as antifungal of Malassezia furfur Abstract Moringa leaf content (Moringa oleifera L) is known to be efficacious as an antifungal. The aim of this research is to formulate and to know gel antifungal activity containing moringa leaf against Malasezia furfur fungus. Moringa leaf extraction was done using 70% ethanol by maceration method. The gel formulation containing ethanol extract of moringa was conducted using hydroxy propyl methyl cellulose (HPMC) with various concentrations, ie 2, 3, and 4%. Evaluation of gel performed was organoleptic, homogeneity, pH, spreading, and viscosity. Then the antifungal activity was tested using solid diffusion method with paper disc, and used ketoconazole gel as comparison. The measured parameter was the antifungal block zone. The results were then analyzed statistically using Kruskall Wallis and Mann Withney. Based on the gel evaluation, it was found that the increased concentrations of HPMC 2, 3, and 4% base concentrations in the gel preparation of ethanol extract of moringa leaf can increase gel viscosity, gel adhesiveness, decrease the gel power, and influence the consistency of preparation for organoleptic test without affecting the test pH gel and gel homogeneity. Formula which have good physical properties of gel when viewed from the test results of physical properties of gel which include organoleptic test, homogeneity, pH, viscosity sticky power and viscosity is gel gel ethanol extract leaf kelor with 2% HPMC base concentration because almost of all test meet the literature requirements. From this study, it can be concluded that leaf extract gel ethanol gel has antifungal activity of M.furfur and gel containing HPMC 2% has gel physical properties and antifungal activity is better when compared with HPMC 3 and 4% concentration gel Keywords: Moringa olifera L.,gel, antifungal, Malasezia furfur

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A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7302 ◽

2018 ◽

Vol 7 (3) ◽

pp. 230-241

Author(s):

Savita Joshi ◽

◽

Parikshit Kumar ◽

Prabha Pant ◽

SC Sati ◽

...

Keyword(s):

Antifungal Activity ◽

Fungal Pathogens ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Total Activity ◽

Maximum Activity ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Cedrus Deodara ◽

Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

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ANALYSIS OF SECONDARY METABOLITES, ANTIBACTERIAL ACTIVITY AND COMPOUND COMPOSITION IN THE SEA CUCUMBER Bohadschia sp. EXTRACT

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v8i2.15831 ◽

2017 ◽

Vol 8 (2) ◽

pp. 645-653 ◽

Cited By ~ 1

Author(s):

Abdullah Rasyid

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Secondary Metabolites ◽

Sea Cucumber ◽

Similarity Index ◽

Diffusion Method ◽

Composition Analysis ◽

Activity Test ◽

Agar Diffusion Method ◽

Color Reactions

Bohadschia sp. is one of the sea cucumber species that has potential to be developed as a source of antibacterial from the sea. Samples of sea cucumber Bohadschia sp. used in this study collected from the Ratai bay waters, Lampung. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition analysis containing in the sea cucumber extract. Identification of secondary metabolites by observation of color reactions, precipitation and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatogaphy-Mass Spectroscopy (GC-MS) method.Top of FormThe results showed that the type of secondary metabolites contained in the extract of sea cucumber Bohadschia sp. were steroids and saponins. The extract of sea cucumber Bohadschia sp. showed antibacterial activity against Bacillus subtilis and Vibrio eltor. Results of GC-MS were 12 compounds and have a similarity index same or more than 90%. All compounds consist of organosilicon cyclic, fatty acid, steroid, cyclo alkene and alkena. The compound with biggest abundance was cholest-5-en-3-yl nonanoate (4.89%) and retention time was 37.370 minutes.

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ANTIFUNGAL ACTIVITY OF PIMENTA DIOICA (L.) MERRIL AN AROMATIC MEDICINAL TREE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i10.9924 ◽

2016 ◽

Vol 8 (10) ◽

pp. 92 ◽

Cited By ~ 2

Author(s):

Akhilesh Bhat ◽

Raveesha K. A.

Keyword(s):

Antifungal Activity ◽

Petroleum Ether ◽

Petroleum Ether Extract ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Microsporum Canis ◽

Ether Extract ◽

Disc Diffusion Method ◽

Rf Values ◽

Pimenta Dioica

Objective: To assess the antifungal activities of the extracts of leaves of Pimenta dioica against human pathogenic fungi and identify the class of phytochemical responsible for the biological activity.Methods: The shade dried, and powdered leaves of Pimenta dioica were subjected to polarity based solvent extraction by soaking. The extracts were dried in a rotary flash evaporator and subjected to antifungal activity assay against Candida albicans, Microsporum canis and M. gypseum, by disc diffusion method. Antifungal active petroleum ether extract was subjected to phytochemical analysis to identify the active compound. The extract was then subjected to Thin Layer Chromatography (TLC) and Bioautography, by agar overlay method. The fractions were collected by Preparative TLC method and subjected to antifungal activity assay by disc diffusion method. Further, the Libermann-Burchard test was conducted to detect the broad group of compounds responsible for the bioactivity.Results: Among the five extracts viz. petroleum ether, chloroform, ethyl acetate, methanol and aqueous, the petroleum ether extract showed significant antifungal activity against Microsporum canis (50 mm), Microsporum gypseum (60 mm) and Candida albicans (23 mm). Terpenoids, tannins and flavonoids were found to be the chief constituents of the petroleum ether extract. The TLC of the extract revealed 7 spots with the Rf values 0.37, 0.42, 0.45, 0.52, 0.59, 0.76, 0.96 with the solvent system of hexane and acetone (1:0.5). The bioautography assay revealed inhibitory activity in the spots at Rf values 0.52 and 0.59. These fractions obtained by preparative TLC also confirmed the same. The phytochemical analysis of the fractions on the TLC plate suggests that the compound is triterpene or steroid.Conclusion: The antifungal activity of Pimenta dioica and the bioactive phytochemical has been reported in the present study. Preliminary phytochemical analysis has indicated that the compounds are triterpenes or steroids. The results indicate that the active compounds are potential lead molecules for new antifungal drugs in the management of infectious fungi.

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Uji Daya Hambat Ekstrak Etanol Batang Saluang Belum (Luvunga sarmentosa Kurz) Terhadap Bakteri Propionibacterium acnes

Jurnal Pharmascience ◽

10.20527/jps.v8i1.9111 ◽

2021 ◽

Vol 8 (1) ◽

pp. 65

Author(s):

Rezqi Handayani ◽

Nurul Qamariah ◽

Muhammad Izmiansyah

Keyword(s):

Secondary Metabolites ◽

Inhibitory Activity ◽

Propionibacterium Acnes ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Inhibition Test ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Percolation Method

Saluang Belum (Luvunga sarmentosa Kurz) adalah tumbuhan yang dikenal sebagai obat tradisional di Kalimantan Tengah. Batang L. sarmentosa Kurz dipercayai memiliki manfaat secara empiris sebagai antioksidan yang dimanfaatkan sebagai anti-aging (anti penuaan dini). Batang L. sarmentosa Kurz mengandung metabolit sekunder yaitu tanin, saponin, steroid dan flavonoid yang diduga memiliki aktivitas sebagai antibakteri. Berdasarkan khasiat empiris dan kandungan metabolit sekunder yang ada dalam tumbuhan L. sarmentosa Kurz, maka dilakukan penelitian tentang aktivitas daya hambat ekstrak etanol tumbuhan ini terhadap bakteri P. acnes yang merupakan salah satu bakteri penyebab jerawat. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas daya hambat ekstrak etanol batang L. sarmentosa Kurz terhadap bakteri P.acnes. Metode penelitian yang dilakukan pada penelitian ini dimulai dari pengambilan simplisia, pembuatan ekstrak etanol dengan metode perkolasi dan uji daya hambat dengan menggunakan metode Disk Difusion yang menggunakan kontrol positif antibiotik klindamicin. Analisis data dilakukan dengan membandingkan lebar zona hambat dari ekstrak etanol batang L. sarmentosa Kurz dengan kategori penghambatan antimikroba klindamisin berdasarkan diameter zona hambat dari Clinical and Laboratory Standards Institute. Hasil uji daya hambat didapatkan zona hambat ekstrak etanol batang Saluang Belum pada semua konsentrasi 0,5%, 1%, 5%, 10% dan 15% secara berturut-turut yaitu Intermediate, Resistant, Intermediate, Susceptible dan Susceptible dan hasil dari zona hambat klindamicin pada semua konsentrasi 0,5%,1%,5%,10%,dan 15% adalah susceptible. Simpulan dari penelitian ini adalah ekstrak etanol batang L. sarmentosa Kurz mempunyai kemampuan dalam menghambat pertumbuhan bakteri P.acnes. Kata Kunci: Obat Tradisional, Jerawat, Uji Daya Hambat, Propionibacterium acnes, Batang Saluang Belum Saluang belum (Luvunga sarmentosa Kurz) is one of traditional medicine in Central Kalimantan. The stem of L. sarmentosa Kurz is believed to have empirical benefits as an antioxidant which is used as anti-ageing (anti-ageing). The stem of L. sarmentosa Kurz contains secondary metabolites, namely tannins, saponins, steroids and flavonoids which are thought to have antibacterial activity. Based on the empirical properties and the content of secondary metabolites in L. sarmentosa Kurz, a study was conducted on the inhibitory activity of this plant's ethanol extract against P. acnes, which is one of the bacteria that causes acne. The purpose of this study was to determine the inhibitory activity of the ethanol extract of L. sarmentosa Kurz stem against P.acnes bacteria. The research method carried out in this study started from taking simplicia, making ethanol extract using the percolation method and the inhibition test using the disk diffusion method which used a positive control of the clindamycin antibiotic. Data analysis was performed by comparing the inhibition zone width of the ethanol extract of L. sarmentosa Kurz stem with the clindamycin antimicrobial inhibition category based on the inhibition zone diameter of the Clinical and Laboratory Standards Institute. The results of the inhibition test obtained the inhibition zone of Saluang Belum stem ethanol extract at all concentrations of 0.5%, 1%, 5%, 10% and 15% respectively, Intermediate, Resistant, Intermediate, Susceptible and Susceptible and the results of the inhibition zone. Clindamycin at all concentrations of 0.5%, 1%, 5%, 10%, and 15% are susceptible. This research concludes that the ethanol extract of L. sarmentosa Kurz stem can inhibit the growth of P.acnes bacteria.

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Uji Daya Hambat Formula Gel Ekstrak Etanol Daun Murbei (Morus alba L.) Sebagai Anti Acne Terhadap Bakteri Propionibacterium acne.

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2019.v5.i2.13663 ◽

2019 ◽

Vol 5 (2) ◽

pp. 206-213

Author(s):

Ahmad Irsyad Aliah ◽

Wahyuni Wahyuni ◽

Nurjannah Bachri

Keyword(s):

Leaf Extract ◽

Ethanol Extract ◽

Bacterial Activity ◽

Morus Alba ◽

Diffusion Method ◽

Clear Zone ◽

Propionibacterium Acne ◽

Mulberry Leaf ◽

Mulberry Leaf Extract ◽

Agar Diffusion Method

A research on the inhibition of formula gel test ethanol extract of leaves of mulberry (Morus alba L.) as an anti-acne against bacteria Propionibacterium acne. This study uses a gel formulation made using mulberry leaf extract (Morus alba L.), which aims to determine the inhibition of antibacterial gel mulberry leaf extract (Morus alba L.) against the bacteria Propionibacterium acne. Gel made three dosage concentrations of 2%, 4% and 6%. Testing inhibition of antibacterial gel agar diffusion method. Test Antibacterial inhibition obtained by looking at the clear zone in the medium and measured using calipers. The results showed that the inhibition of antibacterial gel formulation with a concentration of 2%, 4% and 6% showing that inhibiting bacterial activity.

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UJI DAYA HAMBAT ANTIBAKTERI SALEP EKSTRAK ETANOL DAUN PANDAN HUTAN (Freycinetia sessiliflora Rizki.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan ◽

10.36387/jiis.v5i2.530 ◽

2020 ◽

Vol 5 (2) ◽

pp. 376-386

Author(s):

Fitri Sri Rizki ◽

◽

Ade Ferdinan

Keyword(s):

Secondary Metabolites ◽

Staphylococcus Epidermidis ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibitory Effect ◽

Water Soluble ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Bacteria Inhibition ◽

Soluble Base

Freycinetia sessiliflora Rizki is a plant that has secondary metabolites, namely alkaloids, flavonoids, terpenoids-steroids, saponins, phenols and tannins. This study aims to determine whether there is inhibitory and concentrated ointment extract of thick pandanus leaf of the forest that can inhibit the growth of Stapylococcus epidermidis bacteria. Inhibition testing was carried out by disk diffusion method with different concentrations of 5% and 10% concentrations made 9 ointment formulas with a variety of 3 bases namely hydrocarbon base, absorbency base and water soluble base. The results of inhibitory measurements of ethanol extract ointment leaves of pandanus leaves are good or have the greatest value to the smallest inhibitory effect on the growth of Staphylococcus epidermidis bacteria with an average diameter of inhibition at F6 of 4.11 mm, F1 of 2.78 mm, F3 of 2.68 mm, F5 of 2.47 mm, F4 of 2.25 mm, F2 of 2.23 mm, F7 of 1.29, F8 of 1.13 and F9 of 0.86 mm.

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