scholarly journals THE MICROCIRCULATION PARAMETERS IN PATIENTS WITH ODONTOGENIC INFLAMMATORY DISEASES OF MAXILLOFACIAL AREA

2015 ◽  
pp. 46-49
Author(s):  
A. A. Kabanova ◽  
V. I. Kozlovskiy
Keyword(s):  
Blood Plasma ◽  
Inflammatory Diseases ◽  
Erythrocyte Deformability ◽  
Hospitalized Patients ◽  
Similar Comparison ◽  
Deformability Of Erythrocytes ◽  
Healthy Donors ◽  
Comprehensive Survey ◽  
Healthy Persons

The aim of the study was to determine changes of certain microcirculation parameters in patients with odontogenic inflammatory diseases of maxillofacial area. Material and methods. The study included the results of a comprehensive survey of 31 patients with infectious and inflammatory diseases of maxillofacial area of odontogenic etiology and 30 healthy donors. We studied the deformability of erythrocytes and aggregation of the suspension of leukocytes and platelets. Results. The parameters of the level and speed of the aggregation of the suspension of leukocytes and platelets in the development of infectious inflammatory diseases of maxillofacial area of odontogenic pathology increase in regard to those in healthy persons. At the same time, the similar comparison of the erythrocyte deformability in patients reliably decrease. Conclusion. There is a decrease in the erythrocyte deformability and increase in the rate and degree of aggregation of the suspension of leukocytes and platelets with the development of odontogenic infectious and inflammatory diseases in maxillofacial area. During the treatment of the patients the microcirculation abnormalities are preserved. The index of erythrocytes deformability in the blood plasma rises towards the end of the treatment relative to the values determined for just hospitalized patients.

scholarly journals Carriage ofStaphylococcus aureusamong 104 healthy persons during a 19-month period

1995 ◽  
Vol 115 (1) ◽  
pp. 51-60 ◽  
Author(s):  
N. H.Riewerts Eriksen ◽  
F. Espersen ◽  
V.Thamdrup Rosdahl ◽  
K. Jensen
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Statistical Difference ◽  
Phage Type ◽  
Hospitalized Patients ◽  
Index Number ◽  
Carrier Rate ◽  
Type Distribution ◽  
Nasal Carrier ◽  
Healthy Persons

SummaryThe present study was undertaken to investigate the frequency of the nasal carrier rate ofStaphylococcus aureus. The investigation was performed on 104 healthy persons. The total number of swabs performed was 1498 and this resulted in isolation of 522S. aureusstrains. All strains have been identified, tested for antibiotic susceptibility, and phage-typed. The carrier-index (number of positive swabs/number of total swabs for each individual person) was compared with different sampling and culturing methods, phage type, age, and resistance to antibiotics. There was statistical difference in carrier rate according to sex (P·05). Among the 104 persons 15 (14·4%) were persistent carriers, 17 (16·3%) intermittent carriers, 55 (52·9%) occasional carriers and 17 (16·3%) non-carriers. Among intermittent and occasional carriers the phage-type distribution was different from theS. aureusstrains isolated from Danish hospitalized patients in 1992, while the persistent carriers had similar phage-type distribution.

scholarly journals The Use of Natural Agents to Counteract Telomere Shortening: Effects of a Multi-Component Extract of Astragalus mongholicus Bunge and Danazol

Biomedicines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 31 ◽  
Author(s):  
Isabelle Guinobert ◽  
Claude Blondeau ◽  
Bruno Colicchio ◽  
Noufissa Oudrhiri ◽  
Alain Dieterlen ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Telomerase Activity ◽  
Peripheral Blood Lymphocytes ◽  
Root Extract ◽  
Telomere Shortening ◽  
Patients With Cancer ◽  
Natural Agents ◽  
Age Related ◽  
Healthy Donors ◽  
Aging People

A link between telomere shortening and oxidative stress was found in aging people and patients with cancer or inflammatory diseases. Extracts of Astragalus spp. are known to stimulate telomerase activity, thereby compensating telomere shortening. We characterized a multi-component hydroethanolic root extract (HRE) of Astragalus mongholicus Bunge and assessed its effects on telomeres compared to those of danazol. Astragalosides I to IV, flavonoids, amino acids and sugars were detected in the HRE. Samples of peripheral blood lymphocytes with short telomeres from 18 healthy donors (mean age 63.5 years; range 32–86 years) were exposed to a single dose of 1 µg/mL HRE or danazol for three days. Telomere length and telomerase expression were then measured. Significant elongation of telomeres associated to a less toxicity was observed in lymphocytes from 13/18 donors following HRE treatment (0.54 kb (0.15–2.06 kb)) and in those from 9/18 donors after danazol treatment (0.95 kb (0.06–2.06 kb)). The rate of cells with short telomeres (<3 kb) decreased in lymphocytes from all donors after exposure to either HRE or danazol, telomere elongation being telomerase-dependent. These findings suggest that the HRE could be used for the management of age-related diseases.

scholarly journals ITIH5 and ECRG4 DNA Methylation Biomarker Test (EI-BLA) for Urine-Based Non-Invasive Detection of Bladder Cancer

2020 ◽  
Vol 21 (3) ◽  
pp. 1117 ◽  
Author(s):  
Michael Rose ◽  
Sarah Bringezu ◽  
Laura Godfrey ◽  
David Fiedler ◽  
Nadine T. Gaisa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Bladder Cancer ◽  
Inflammatory Diseases ◽  
Cell Detection ◽  
Detection Range ◽  
Non Invasive ◽  
Specific Pcr ◽  
Healthy Donors ◽  
Methylation Sensitive Restriction Enzyme ◽  
Spiking Experiment

Bladder cancer is one of the more common malignancies in humans and the most expensive tumor for treating in the Unites States (US) and Europe due to the need for lifelong surveillance. Non-invasive tests approved by the FDA have not been widely adopted in routine diagnosis so far. Therefore, we aimed to characterize the two putative tumor suppressor genes ECRG4 and ITIH5 as novel urinary DNA methylation biomarkers that are suitable for non-invasive detection of bladder cancer. While assessing the analytical performance, a spiking experiment was performed by determining the limit of RT112 tumor cell detection (range: 100–10,000 cells) in the urine of healthy donors in dependency of the processing protocols of the RWTH cBMB. Clinically, urine sediments of 474 patients were analyzed by using quantitative methylation-specific PCR (qMSP) and Methylation Sensitive Restriction Enzyme (MSRE) qPCR techniques. Overall, ECRG4-ITIH5 showed a sensitivity of 64% to 70% with a specificity ranging between 80% and 92%, i.e., discriminating healthy, benign lesions, and/or inflammatory diseases from bladder tumors. When comparing single biomarkers, ECRG4 achieved a sensitivity of 73%, which was increased by combination with the known biomarker candidate NID2 up to 76% at a specificity of 97%. Hence, ITIH5 and, in particular, ECRG4 might be promising candidates for further optimizing current bladder cancer biomarker panels and platforms.

Protease/protease inhibitor balance in blood plasma to predict postoperative complications in operated patients with pancreatic head cancer.

2018 ◽  
Vol 36 (4_suppl) ◽  
pp. 258-258
Author(s):  
Oleg Ivanovich Kit ◽  
Elena Frantsiyants ◽  
Larisa S. Kozlova ◽  
Valeria A. Bandovkina ◽  
Evgeniy N. Kolesnikov ◽  
...  
Keyword(s):  
Postoperative Complications ◽  
Protease Inhibitor ◽  
Blood Plasma ◽  
Pancreatic Head ◽  
Head Cancer ◽  
Pancreatic Head Cancer ◽  
Postoperative Pancreatitis ◽  
Healthy Donors ◽  
Before And After ◽  
Microsoft Office

258 Background: Our purpose was to study the protease/protease inhibitor (P/PI) balance in the blood plasma of patients with cancer of the head of the pancreas before and after pancreatoduodenal resection (PDR) with postoperative complications. Methods: The study was performed using clinical observation, biochemical examinations and statistical analysis in Microsoft Office Excel 2010. The blood plasma of 92 patients with pancreatic head cancer (53 men and 39 women aged 45-76 years, Т2-4N0M0) was studied before the surgery (b/s) and on days 1, 7, 14 and 17 after PDR. The patients were divided into two groups: g1 – 69 patients without postoperative (p/o) complications and g2 – 23 patients with p/o complications: generalization – 7, thrombosis - 8, acute postoperative pancreatitis - 2, gastrostasis - 2, anastomotic leakage - 4 patients. Kinetics of trypsin-like proteases (TLP) and α-1-proteinase inhibitor (α1PI) was studied by spectrophotometry. The data were compared with the blood plasma of 39 healthy donors (N). Results: TLP activity b/s exceeded N in g1 and g2 by 4.1 and 10.6 times; TLP in g2 was 2.6 times higher than in g1. The α1PI activity b/s was higher than N by 1.2 times (p < 0.05) in g1 and lower than N by 2.0 times in g2; α1PI in g2 was 2.4 times lower than in g1. After PDR, activity of TLP increased in all patients on day 1 but decreased on days 7-14 in g1 remaining 2.7 times higher than N by the discharge. The TLP activity in g2 by the discharge was similar to levels b/s and exceeded g1 by 4.4 and N by 12.1 times. The α1PI activity after PDR increased in all patients on days 1-17, but in g1 by the discharge it was similar to N and in g2 it was 1.5 times lower than N. The TLP/α1PI ratio was higher in g2 than in g1 at all times. Conclusions: A high TLP activity and a low α1PI activity, compared to N, were maintained in the blood plasma of all patients with p/o complications, despite their types. The P/PI balance in g2 was shifted to the left being 5.5-9.0 times higher than in g1 at all times which allowed the prognosis of postoperative complications before the surgery, perioperatively or on day 1 after the surgery.

scholarly journals Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Inessa Skrypkina ◽  
Liudmyla Tsyba ◽  
Kateryna Onyshchenko ◽  
Dmytro Morderer ◽  
Olena Kashparova ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cancer Patients ◽  
Renal Cancer ◽  
Cpg Islands ◽  
Tumor Development ◽  
Diagnostic Markers ◽  
Cell Free Dna ◽  
Free Dna ◽  
Healthy Donors ◽  
Fluorescence Test

The critical point for successful treatment of cancer is diagnosis at early stages of tumor development. Cancer cell-specific methylated DNA has been found in the blood of cancer patients, indicating that cell-free DNA (cfDNA) circulating in the blood is a convenient tumor-associated DNA marker. Therefore methylated cfDNA can be used as a minimally invasive diagnostic marker. We analysed the concentration of plasma cfDNA and methylation of six tumor suppressor genes in samples of 27 patients with renal cancer and 15 healthy donors as controls. The cfDNA concentrations in samples from cancer patients and healthy donors was measured using two different methods, the SYBR Green I fluorescence test and quantitative real-time PCR. Both methods revealed a statistically significant increase of cfDNA concentrations in cancer patients. Hypermethylation on cfDNA was detected for theLRRC3B(74.1%),APC(51.9%),FHIT(55.6%), andRASSF1(62.9%) genes in patients with renal cancer. Promoter methylation ofVHLandITGA9genes was not found on cfDNA. Our results confirmed that the cfDNA level and methylation of CpG islands ofRASSF1A,FHIT, andAPCgenes in blood plasma can be used as noninvasive diagnostic markers of cancer.

scholarly journals Investigation of platelet aggregation in patients with laryngeal cancer

2016 ◽  
Vol 71 (1) ◽  
pp. 20-23
Author(s):  
Yu. Burlaka ◽  
N. Gryn ◽  
S. Verovka
Keyword(s):  
Platelet Aggregation ◽  
Comparative Study ◽  
Blood Plasma ◽  
Laryngeal Cancer ◽  
Number Of Patients ◽  
Platelet Hyperaggregation ◽  
Healthy Persons

A comparative study of the induced platelet aggregation in patients with laryngeal cancer to determine the most revealing informative violations. It was compared evaluation of results of platelet aggregation in the blood plasma of patients with laryngeal cancer compared to the healthy persons. It was found intensification of ADP induced platelet aggregation in the concentration range which was used. At the same time with the increase the number of patients with platelet hyperaggregation reliable platelet decrease in blood and increase of patients with thrombocytopenia at the III-rd stage of laryngeal cancer are observed. In patients with lI-nd and III-rd stage of laryngeal cancer was found increase in the level, rate and aggregation time compared to the healthy persons. The most significant violations observed in the II-nd stage of the cancer process.

scholarly journals Peculiarities of Free-Radical Processes and Neuropsychological Status in Patients with Chronic Generalized Periodontitis and Possibility of Correcting their Impairments with Antioxidant Therapy

2019 ◽  
Vol 20 (2) ◽  
pp. 55-64
Author(s):  
Mihail Saulin ◽  
Sergej Bolevich ◽  
Tatjana Savateva-Liubiova ◽  
Konstantin Sivak ◽  
Ekaterina Silina
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Plasma ◽  
Malonic Dialdehyde ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Healthy Donors ◽  
Surgical Methods ◽  
Antiperoxide Activity ◽  
In Blood Plasma

Abstract This clinical study included a total of 71 patients with chronic generalized periodontitis (CGP). The chemiluminescence method was used to determine the level of generation of reactive oxygen species by leukocytes (basal and stimulated), as well as the content of malonic dialdehyde in blood plasma and antiperoxide activity of plasma. The anxiety level was measured using the Spielberger’s test. Patients with chronic generalized periodontitis in the exacerbation phase were found to have a natural significant increase in both basal and stimulated PICL, as well as an increase in blood plasma malonic dialdehyde 1.6-, 3.9- and 1.4-fold, respectively, compared with apparently healthy donors (all p<0.05), and a significant 2.1-fold decrease in plasma APA compared with healthy donors (p<0.001). In the remission phase, all parameters were normalized, however, they did not reach the norm. We revealed significant dependence of reactive oxygen species generation by leukocytes, lipid peroxidation and antiperoxide activity of plasma on the severity of the course of chronic generalized periodontitis, whereas plasma antiperoxide activity decreased. In patients with the severe course, after the treatment with AO, situational and personal anxiety increased, depression deteriorated, unlike patients with mild-to-moderate course in whom this symptomatology virtually disappeared. In patients with the severe course of periodontitis after surgical manipulations, the parameter in the Hounsfield units increased gradually, on days 14, 30 and 90 on average, 1.3, 2.4 and 3.2-fold, respectively, thus suggesting that bone tissue density increased faster after surgical methods of treatment in patients with severe chronic generalized periodontitis.

scholarly journals SARS-CoV-2 serological analysis of COVID-19 hospitalized patients, pauci-symptomatic individuals and blood donors

2020 ◽  
Author(s):  
Ludivine Grzelak ◽  
Sarah Temmam ◽  
Cyril Planchais ◽  
Caroline Demeret ◽  
Christèle Huon ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Donors ◽  
Hospitalized Patients ◽  
High Antibody ◽  
Serological Analysis ◽  
Healthy Donors ◽  
Response Profile ◽  
Antibody Titers ◽  
Antibody Profiling ◽  
Nucleoprotein N

AbstractIt is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their antibody response profile. Here, we performed a pilot study to assess the levels of anti-SARS-CoV-2 antibodies in samples taken from 491 pre-epidemic individuals, 51 patients from Hôpital Bichat (Paris), 209 pauci-symptomatic individuals in the French Oise region and 200 contemporary Oise blood donors. Two in-house ELISA assays, that recognize the full-length nucleoprotein (N) or trimeric Spike (S) ectodomain were implemented. We also developed two novel assays: the S-Flow assay, which is based on the recognition of S at the cell surface by flow-cytometry, and the LIPS assay that recognizes diverse antigens (including S1 or N C-terminal domain) by immunoprecipitation. Overall, the results obtained with the four assays were similar, with differences in sensitivity that can be attributed to the technique and the antigen in use. High antibody titers were associated with neutralisation activity, assessed using infectious SARS-CoV-2 or lentiviral-S pseudotypes. In hospitalized patients, seroconversion and neutralisation occurred on 5-14 days post symptom onset, confirming previous studies. Seropositivity was detected in 29% of pauci-symptomatic individuals within 15 days post-symptoms and 3 % of blood of healthy donors collected in the area of a cluster of COVID cases. Altogether, our assays allow for a broad evaluation of SARS-CoV2 seroprevalence and antibody profiling in different population subsets.

scholarly journals Prevalence and Causes of Anemia in Hospitalized Patients: Impact on Diseases Outcome

2020 ◽  
Vol 9 (4) ◽  
pp. 950 ◽  
Author(s):  
Maria Luigia Randi ◽  
Irene Bertozzi ◽  
Claudia Santarossa ◽  
Elisabetta Cosi ◽  
Fabrizio Lucente ◽  
...  
Keyword(s):  
Internal Medicine ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Inflammatory Diseases ◽  
Specific Treatment ◽  
Hospitalized Patients ◽  
Medical Attention ◽  
Loss Of Function ◽  
Discharge Letter ◽  
A Minor

Anemia is extremely common in hospitalized patients who are old and often with multiple diseases. We evaluated 435 consecutive patients admitted in the internal medicine department of a hub hospital and 191 (43.9%) of them were anemic. Demographic, historic and clinical data, laboratory tests, duration of hospitalization, re-admission at 30 days and death were recorded. Patients were stratified by age (<65, 65–80, >80 years), anemia severity, and etiology of anemia. The causes of anemia were: iron deficiency in 28 patients, vitamin B12 and folic acid deficiencies in 6, chronic inflammatory diseases in 80, chronic kidney disease in 15, and multifactorial in 62. The severity of the clinical picture at admission was significantly worse (p < 0.001), length of hospitalization was longer (p < 0.001) and inversely correlated to the Hb concentration, re-admissions and deaths were more frequent (p 0.017) in anemic compared to non-anemic patients. A specific treatment for anemia was used in 99 patients (36.6%) (transfusions, erythropoietin, iron, vitamin B12 and/or folic acid). Anemia (and/or its treatment) was red in the discharge letter only 54 patients. Even if anemia is common, in internal medicine departments scarce attention is paid to it, as it is generally considered a “minor” problem, particularly in older patients often affected by multiple pathologies. Our data indicate the need of renewed medical attention to anemia, as it may positively affect the outcome of several concurrent medical conditions and the multidimensional loss of function in older hospitalized patients.

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