scholarly journals Bioactive compounds and antioxidant potential of soft wheat and oat bran on the Algerian market

Author(s):  
Samira Meziani ◽  
Souad Saidani ◽  
Lahouaria Labga ◽  
Rawda Benguella ◽  
Imene Bekhaled

Background: Rich in dietary fibers and beneficial to health, wheat and oats have been a popular part of the human diet. The whole grain is rich in protein, lipid, starch and phenolic compounds concentrated at the level of the peripheral layer of the bran. Aims: The natural compounds and the antioxidant potential of two different species of soft wheat and oat bran on the Algerian market have been studied in this work. Wheat bran was furnished by Azzouz’s Cereal and Dried Vegetable Cooperative (CCLS) being the most commercialized oat bran in Algeria. Material and Methods: Some parameters and bran biochemical compounds such as proteins, cellulose, ash content, phenolics, and antioxidant potential (DPPH) were determined using different techniques and methods (infra-red approach spectrophotometer, and flame spectrophotometer). Results: The results obtained showed that studied soft wheat bran was rich in protein (17.36%). Concerning cellulose, a high value was recorded for this bran variety 11.3%, which was lower for oat bran (2.7%). The maximum concentration of potassium and sodium was observed in the soft wheat bran variety; 3.16 mg/L, 30.36 mg/L respectively. The levels of the phenolic compounds were 0.720 ± 0.050 mg EAG / g and 1.101 ± 0.01mg EAG / g for the oat bran and the soft wheat bran respectively. These results underline that both studied brans contain significant levels of compounds essential for consumer needs. Conclusions: The studied soft wheat bran variety is considered to be an important source of phytonutrients. Keywords: Wheat, oat, antioxidants, radical scavenging, phenolic contents.

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 26
Author(s):  
Vigasini Subbiah ◽  
Biming Zhong ◽  
Malik A. Nawaz ◽  
Colin J. Barrow ◽  
Frank R. Dunshea ◽  
...  

Berries are grown worldwide with the most consumed berries being blackberries (Rubus spp.), blueberries (Vaccinium corymbosum), red raspberries (Rubus idaeus) and strawberries (Fragaria spp.). Berries are either consumed fresh, frozen, or processed into wines, juices, and jams. In recent times, researchers have focused their attention on berries due to their abundance in phenolic compounds. The current study aimed to evaluate the phenolic content and their antioxidant potential followed by characterization and quantification using LC-ESI-QTOF-MS/MS and HPLC-PDA. Blueberries were highest in TPC (2.93 ± 0.07 mg GAE/gf.w.) and TFC (70.31 ± 1.21 µg QE/gf.w.), whereas the blackberries had the highest content in TTC (11.32 ± 0.13 mg CE/gf.w.). Blueberries had the highest radical scavenging capacities for the DPPH (1.69 ± 0.09 mg AAE/gf.w.), FRAP (367.43 ± 3.09 µg AAE/gf.w.), TAC (1.47 ± 0.20 mg AAE/gf.w.) and ABTS was highest in strawberries (3.67 ± 0.14 mg AAE/gf.w.). LC-ESI-QTOF-MS/MS study identified a total of 65 compounds including 42 compounds in strawberries, 30 compounds in raspberries, 28 compounds in blueberries and 21 compounds in blackberries. The HPLC-PDA quantification observed phenolic acid (p-hydroxybenzoic) and flavonoid (quercetin-3-rhamnoside) higher in blueberries compared to other berries. Our study showed the presence of phenolic acids and provides information to be utilized as an ingredient in food, pharmaceutical and nutraceutical industries.


2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 371-371
Author(s):  
Josue Bolanos ◽  
Sun-Ok Lee ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Shahidul Islam ◽  
...  

Abstract Objectives Sweetpotato leaf (SPL) is a natural source of phenolic compounds with potential utility as an antioxidant. The study aimed to measure the impacts of the years on SPL total phenolic content and antioxidant capacity and to identify and quantify the individual phenolic compounds. Methods Sweetpotato leaves in 2018 (27 varieties) and 2019 (24 varieties) were grinded and lyophilized. Phenolic compounds were extracted with 70% ethanol from SPLs. Total phenolic content was determined by Folin-Ciocalteu method and antioxidant potential was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Five SPL samples with statistically highest antioxidant capacity were identified and quantified by HPLC. All statistical analyses were carried out by SAS software using ANOVA. Statistical significance was accepted at P < 0.05. Results The average of total phenolic contents in 2018 SPL samples was 43 ± 13 mg gallic acid equivalent (GAE)/g dry weight (DW) SPL whereas in 2019, it was 53 ± 9 mg GAE/g dry weight SPL. Antioxidant capacity in 2018 was 110 ± 75 µmol Trolox equivalent (TE)/g dry weight SPL whereas in 2019, it was 132 ± 32 µmol TE/g dry weight SPL. SPL1 had the highest antioxidant potential, followed by SPL9, SPL3, SPL28, and SPL11 (P < 0.05). The concentrations of major identified phenolics from the five SPL samples (#1, 9, 3, 28, and 11) in dry weight of SPL were: chlorogenic acid at 3.05 ± 0.35 mg/g, 5-monocaffeoylquinic acid (CQA) at 0.71 ± 0.06 mg chlorogenic acid equivalent (ChAE)/g, 4-CQA at 0.99 ± 0.07 mg ChAE/g, 3,4-diCQA at 1.22 ± 0.18 mg ChAE/g, 3,4,5-triCQA at 1.2 ± 0.18 mg ChAE/g, and 3,5-diCQA at 15.5 ± 4.05 mg ChAE/g. 3,5-diCQA, the predominant phenolic, was present in the highest amounts in SPL1. Conclusions The results showed that sweetpotato leaves collected in 2019 contained higher total phenolics and antioxidant capacity than the ones collected in 2018. Phenolic compounds have strong antioxidant activity in Arkansas-grown sweetpotato leaves. This study warrants further investigation of sweetpotato leaves to be utilized as an antioxidant. Funding Sources The work was supported by USDA-NIFA.


2017 ◽  
Vol 39 (1) ◽  
pp. 43 ◽  
Author(s):  
Mayara Neves Santos Guedes ◽  
Rafael Pio ◽  
Luana Aparecida Castilho Maro ◽  
Fabíola Fonseca Lage ◽  
Celeste Maria Patto de Abreu ◽  
...  

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested. 


2021 ◽  
Vol 12 (1) ◽  
pp. 877-883
Author(s):  
Rangarajan N ◽  
Sampath V ◽  
Dass Prakash M V ◽  
Mohanasundaram S

Notable growth in the usage of herbal remedies could be seen in recent days as an effective alternative choice of drugs. In particular, phenolic compounds have gained the highest attention and priority among the scientific community in exploring them as a novel drug for a wide range of communicable and non-communicable diseases. It has been studied well and proven extensively that the antioxidant potential of plants is largely depending on its phenolic contents. This study was focused to compare and analysis the biochemical composition, antioxidant activity and characterization of phenolic compounds in Glycyrrhiza glabra  and Zingiber officinale root extracts. The ethanolic and chloroform extracts of Glycyrrhiza glabra  and Zingiber officinale roots  were subjected to preliminary screening. The qualitative Phytochemical tests of the Glycyrrhiza glabra  and Zingiber officinale root showed the existence of polyphenols, alkaloids, saponins, terpenoids, flavanoids, amino acids and proteins. The DPPH assay of Glycyrrhiza glabra and Zingiber officinale root tissues showed a significant antioxidant property. FT-IR and UV Spectral studies of Glycyrrhiza glabra and Zingiber officinale roots showed the presence of different biologically significant flavonoids. The results of this study exemplifies that the selected plants are notable sources of phenolic compounds and hence observed that significant antioxidant potential.


2020 ◽  
Vol 23 ◽  
Author(s):  
Penha Patrícia Cabral Ribeiro ◽  
Francisco Canindé de Sousa Júnior ◽  
Cristiane Fernandes de Assis ◽  
Bruno Oliveira de Veras ◽  
Carlos Eduardo de Araújo Padilha ◽  
...  

Abstract The objective of the present study was to assess the phenolic compounds and antioxidant capacity of faveleira seed and press cake extracts. Phenolic profiles were assessed by Ultra-High Performance Liquid Chromatography (UHPLC). Furthermore, the Total Phenolic Content (TPC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging were evaluated. The faveleira seed and press cake extracts are sources of natural phenolic compounds in human diet and have potent antioxidant activity. Gallic acid was the predominant phenolic compound in seed and press cake extracts. The study showed that faveleira seed and press cake extracts can be considered functional foods as well as a potential interest to the food industry.


2020 ◽  
Vol 12 (3) ◽  
pp. 741-751
Author(s):  
Samira MEZIANI ◽  
Souad SAIDANI ◽  
Noreddine MENADI ◽  
Hayet MEHIDDA ◽  
Mohammed ZAIRI ◽  
...  

From this context we have been interested in biochemical and phytochemical parameters of local and foreign varieties of wheat bran whose purpose is to search to see if there are intraspecific and interspecific varietal differences. The study concerned five varieties of soft wheat bran selected at CCLS Sidi Bel Abbés and consumed in many regions of Algeria. The biochemical compounds of grains (proteins, cellulose, ash content and phytochemical (total phenols, flavonoids and antioxidant activity) were determined in all varieties. The wheat bran, the subject and material of this study, was obtained from whole wheat grains collected from three varieties of soft wheat and used in our case to produce and extract the maximum of soft wheat bran. The varieties used were ‘HD’ (SWBHD) and ‘Anzar’ (SWBA) local variety and one imported, ‘Habbour’ variety (SWBHB). Two samples of marketed soft wheat bran, imported, were provided in this work to compare their nutritional components. Different techniques and methods were used in this experimental study (infra-red approach spectrophotometer, flame spectrophotometer). The results obtained show that ‘Eriad’ soft wheat bran (SWBE) is rich in protein with a variation of 15.78% at 18.07%; and is slightly elevated compared to other samples. Regarding the results obtained for cellulose, a high value was recorded for (SWBTAZ) variety 11.3%, and a lower one for (SWBHB) marketed at a value of 3.6%. The maximum concentration of potassium and sodium was obtained in the SWBAZ variety with a level of 3.16 mg/l and 30.36 mg/l respectively. The evaluation of phytochemicals has shown the presence of considerable amounts of polyphenols and flavonoids qualitatively and quantitatively. SWBAZ variety was very rich in total polyphenols, flavonoids and DPPH respectively with values of (1.101±0.01 mg EAG/g) and (0.174±0.001 EC/g) (1.39±0.01 EAG/g) compared to the different varieties studied. In conclusion, the SWBAZ variety could thus be considered, in our case, as an important source of phytonutrients.


2012 ◽  
Vol 554-556 ◽  
pp. 1103-1109 ◽  
Author(s):  
Gui Zhi Zhang ◽  
Bao Ping Ji ◽  
Gang Chen

Phenolic antioxidants have multiple benefits to human health. Polyphenols are responsible for the antioxidant activity in apples. Antioxidant activities were assessed using the ferric reducing/antioxidant power (FRAP) and 2,2-dipheny l-1-picrylhydrazyl (DPPH) radical scavenging assays in different cultivars and different parts of apples grown in China. The phenolic compounds and antioxidant activities differed significantly among the four apple cultivars. Guoguang had the highest phenolic concentration and antioxidant activity and Golden Delicious had the lowest. The peels had the highest total phenolic and flavonoid contents followed by the cores and flesh. Anthocyanins were detected only in red apple peels. Peel and core had greater antioxidant activities than apple flesh. FRAP values were inversely correlated with phenolic contents, whereas no clear relationship could be observed between DPPH values and phenolic contents. The higher levels of phenolic compounds and antioxidant activities of apple peels and cores than flesh may be of technological interest as a valuable source of antioxidants.


2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Irene Gouvinhas ◽  
Raúl Domínguez-Perles ◽  
Amadeo Gironés-Vilaplana ◽  
Teresa Carvalho ◽  
Nelson Machado ◽  
...  

Olive fruits, as well as their corresponding oil, represent an interesting source of phytochemicals, mainly phenolic compounds, which arise as secondary metabolites, resulting from the plant’s response to biotic and abiotic stresses. Therefore, olive fruits from three distinct cultivars (“Cobrançosa,” “Galega Vulgar,” and “Picual”) grown in Portugal and displaying different degree of resistance to biotic and abiotic stresses have been studied in relation to the concentration of total phenolic compounds, orthodiphenols and flavonoids, besides antioxidant capacity (DPPH, ABTS, FRAP, and ORACFL), in three maturation stages and two distinct harvest seasons (2012-2013). Generally, a decrease of all phenolic contents throughout the maturation stages has been observed, while, concerning cultivar, green olives of “Cobrançosa” showed the highest values for all contents assessed, denoting a strong influence of the genetic background. The same trend has not been observed regarding antioxidant activity, since Cobrançosa and Galega Vulgar cultivars presented the highest values only for the DPPH and ORACFL assays. Moreover, multivariate analyses pointed to the preponderance of the cultivars’ phenolic composition in the semiripe stage for the resistance to biotic stress, with “Galega” the most susceptible cultivar, presenting the lowest contents at this maturation stage, whereas “Picual” displayed the most pronounced phytochemical response.


Author(s):  
Muhammad Younus ◽  
Muhammad Mohtasheem ul Hasan ◽  
Shakeel Ijaz ◽  
Razia Riaz ◽  
Khalil Ahmad ◽  
...  

Euphorbia nivulia (EN) one of the members of Euphorbiaceae family, is a medicinal plant of Cholistan Desert (Punjab, Pakistan) that is traditionally used for a number of diseases. The plant is enriched with many phyto-constituents including flavonoids, triterpenes and polyphenols. In present study, crude extract as well as various fractions were assessed for phytochemical analysis, HPLC profiling, radical scavenging property and total phenolic and flavonoid contents. Hydro-alcoholic (70%) crude extract of EN was subjected to fractionation using different solvents including n-hexane, chloroform, butanol and aqueous. After performing the phytochemical screening and HPLC profiling, antioxidant activity was estimated by using DPPH and FRAP assays. Total flavonoid and phenolic contents were also estimated. HPLC analysis confirmed the presence of flavonoids and polyphenols like quercitin, gallic acid, caffeic acid, vanillic acid, benzoic acid, chlorogenic acid, syringic acid and ferulic acid in crude extract as well as butanol and aqueous fractions. Results revealed that butanol fraction showed maximum phenolic (143.26±2.65 mg/g GA/g) and crude extract showed maximum flavonoid (69.80±1.212 mg/g Q) contents. Maximum antioxidant potential was displaced by butanol fraction which was IC50=0.04±0.02 by DPPH and 1193.77±12.4 μmol TE/ml by FRAP respectively. Current study is the first information about the HPLC profiling of phenolic compounds in EN and its antioxidant potential that could be a step forward towards ethno-pharmacological based phyto-medicine.


2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.


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