scholarly journals High dilutions of Magonia pubescens hidrogel affect germination variables in Sorghum bicolor L. Moench

2021 ◽  
Vol 10 (36) ◽  
pp. 253-258
Author(s):  
Carlos Moacir Bonato ◽  
Beatriz Rigon ◽  
Aleandro Ferreira Souza ◽  
Carolina Bertuzzi Pereira ◽  
Bruno Reis
Keyword(s):  
Experimental Design ◽  
Distilled Water ◽  
Average Speed ◽  
Germination Process ◽  
Germination Speed ◽  
High Dilutions ◽  
Petri Dishes ◽  
Mother Tincture ◽  
Sorghum Bicolor L ◽  
Germinated Seeds

Introduction: In science homeopathic diseases or physiological disorders are not considered just a result of abiotic and biotic factors, but rather a consequence of loss of organic system homeostasis. Homeopathic science is currently being used efficiently in the control of plagues[1], plant diseases[2], in the increase of medicinal plants’ active principles[3] and in plant metabolism[4,5]. Although actual results, both in the academic and field-level, very little is known about physiological mechanisms action of homeopathic medicine on germination process[6]. This work aims to study the effect of M. pubescens hydrogel, on some physiological variables of sorghum germination (Sorghum bicolor L. Moench). Material and methods: The experiment was conducted at Homeopathy and Plant Physiology of Biology Department at UEM in the period from 04/05/06 to 30/12/06. M. pubescens (tingui) seeds were obtained from the region of Montes Claros - Minas Gerais. The M. pubescens hidrogel was obtained from the external centrals wrappers of 4 dry seeds, after they have been disposed in petri dishes with distilled water for a period of 36 hours of soaking (25oC). The hydrogel mother tincture was prepared according to Manual of Technical Standards for Homeopathic Drugstore[7] 3rd ed (2003), in the proportion of a hidrogel part (5g) to ten parts (50g) of absolute alcohol 70% and stored in a glass amber (capped and protected from light). After 15 days of maceration, the solution was filtered and after 48h at rest, the mother tincture was considered ready for use. The dilution 1cH (Centesimal Hahnemannian) was obtained by adding 0.2 ml of the mother tincture in 19.8 ml of distilled water (1/100) and sucussioned 100 times (33 sucussions s-1) by mechanical arm dynamizer with automatic stop (Model Denise 50 - AUTIC). The subsequent dilutions (2cH to 30cH) were obtained from the same procedure, starting from the dilution 1cH. Bioassay: In petri dish containing 15 seeds of sorghum in a circular distributed were added 10 ml with their dilutions (3, 6, 9, 12, 15, 20 and 30cH) and the control containing distilled water. The petri dishes were placed in a growth chamber (type BOD), temperature of (25 ± 2)°C and photoperiod of 16h. The variables were analyzed by germination period of 73.5h as described below: · Germination (%G): %G = (∑ni.N-1)x100 , where ∑ni, is the total number of germinated seeds in relation to the number of seeds put to germinate, expressed in percentage; · Germination average time (GAT): GAT = ∑ni . ti / ∑ni , where ni is the number of germinated seeds within a certain interval of time ti-1 and ti; expressed in hours. · Germination average speed (GAS), expressed in hours): GAS = ∑ni / ∑ni . ti · Germination speed index (GSI): IVG = G1 / N1 + G2 / N2 + ........ Gn / Nn , where G1, G2....Gn is the number of germinated seeds and N1, N2, ... Nn is the number of hours after sowing. The total number of germinated seeds, at each time (12h) was also analyzed. Seeds were considered germinated when the radicle had 1 to 2 mm of lengths. Experimental design: The experimental design was randomized block with 4 replications, totaling 32 experimental units. It was adopted the double-blind methodology, to avoid possible interference or direction by the researcher. Statistical Analysis: The data were analyzed by ANOVA and the averages compared by Scott-Knott test (p≤0.05). The twinning combined data were analyzed for interaction germination x time (G x T) by F test to 5% of probability. Results and discussion: The homeopathy of Magonia pubescens hydrogel affected on the germination kinetic variables of sorghum seeds, when compared with the control (Fig. 1). This effect was most observed in the initial process of germination (from 13h). Research accomplished by Salgado-Labouriau (1973) [8] showed that the hydrogel formed from the external wrapper Magonia pubescens seeds, does not contain inhibiting, but contains factors that accelerate the germination process. Apparently, these results seem contradictory. However, for the homoeopathic optics, some used medicines in a considered way might have determined effect. Already in high diluted doses this behavior can be reversed, as it happens with some drugs. This behavior in pharmacokinetics is denomined Hormesis. When diluted and given dynamism, the product of hydrogel, instead of stimulating, it can delay the germination for the same phenomenon. Hormesis is not yet explained by science. Homeopathy of the Magonia pubescens gel significantly increased the germination average time (GAT) of sorghum seeds and reduced the germination average speed (GAS) and the germination speed index (GSI) (Fig. 2A, B and C). The values of these variables suggest that homeopathy, somehow slowed the speed of sorghum seeds soaking. Conclusion: The results here presented suggest that high dilutions of Magonia pubescens hidrogel can be used in future experiment such as bioherbicide.

scholarly journals Substrate but not seed size affects seed germination of Dypsis onilahensis (Jum. & H. Perrier) Beentje & J. Dransf

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4193
Author(s):  
Petterson Baptista da Luz ◽  
Thalita Neves Marostega ◽  
Bruno Nicchio ◽  
Leonarda Grillo Neves
Keyword(s):  
Seed Size ◽  
Germination Percentage ◽  
Palm Tree ◽  
Tukey Test ◽  
Germination Process ◽  
Germination Speed ◽  
Randomized Experimental Design ◽  
Sowing Seed ◽  
Substrate Types ◽  
Germinated Seeds

Dypsis onilahensis, commonly known as sibara palm, is a palm tree with enormous ornamental potential. Commercial propagation occurs through seeds, but few studies have evaluated the factors that affect the germination process of this species. Thus, we aimed at evaluating the effects of seed size and substrate on D. onilahensis germination. We used a completely randomized experimental design and a 2 x 3 factorial scheme with two seed sizes (> 15 mm and < 15 mm in diameter) and three substrate types (sand, Plantmax®, and vermiculite), with four replications of 15 seeds. We analyzed germination percentage (G%), number of germinated seeds (NGS), and germination speed index (GSI). Data underwent variance analysis, and means were compared by the Tukey test at 5% probability. Germination began 73 days after sowing. Seed size had no effect on germination. Among substrates, vermiculite showed the best results for GSI, NGS, and G%, followed by sand and then by Plantmax®. Our study provides the first data on factors that affect the germination of this palm tree species.

scholarly journals Temperature on Annona cherimola Mill. x Annona squamosa L. seed germination

2019 ◽  
Vol 5 (2) ◽  
pp. 40
Author(s):  
Deived Carvalho ◽  
Maria Cruz ◽  
Elisete Osipi ◽  
Jethro Osipe ◽  
Ronan Colombo ◽  
...  
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Dry Matter ◽  
Germination Percentage ◽  
Annona Squamosa ◽  
Annona Cherimola ◽  
Germination Speed ◽  
Alternating Temperature ◽  
Seed Propagation ◽  
Germinated Seeds

In the production of atemoya rootstocks (Annona cherimola Mill. x Annona squamosa L.), the seed propagation is hindered by dormancy resulting in low germination, similar to temperature that affects the percentage, speed, and uniformity of germination. This study aimed to evaluate the effect of constant and alternating temperature on the seed germination of three atemoya genotypes: ‘Gefner’, ‘Thompson’ and ‘PR-1’ at 25°C and 20-30°C (8-16h) using germination paper in absence of light, with four samples of 25 seeds per treatment. The evaluations occurred each seven days up to 35 days, which were assessed: germinated seeds, seedlings, dormant seeds and germination speed index. The experimental design was completely randomized in a factorial arrangement of 3x2, using four replications. The water content, fresh and dry matter of 100 seeds were analyzed in a completely randomized scheme with four replications. The alternating temperature 20-30°C provided favorable condition and higher germination percentage for atemoya seeds, achieving 0.638 for germination speed index and 37% for percentage of germination at 35 days. ‘Gefner’ and ‘Thompson’ cultivars presented higher percentage of germination than ‘PR-1’.

scholarly journals Substrate but not seed size affects seed germination of Dypsis onilahensis (Jum. & H. Perrier) Beentje & J. Dransf

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4193
Author(s):  
Petterson Baptista da Luz ◽  
Thalita Neves Marostega ◽  
Bruno Nicchio ◽  
Leonarda Grillo Neves
Keyword(s):  
Seed Size ◽  
Germination Percentage ◽  
Palm Tree ◽  
Tukey Test ◽  
Germination Process ◽  
Germination Speed ◽  
Randomized Experimental Design ◽  
Sowing Seed ◽  
Substrate Types ◽  
Germinated Seeds

<p>Dypsis onilahensis, commonly known as sibara palm, is a palm tree with enormous ornamental potential. Commercial propagation occurs through seeds, but few studies have evaluated the factors that affect the germination process of this species. Thus, we aimed at evaluating the effects of seed size and substrate on D. onilahensis germination. We used a completely randomized experimental design and a 2 x 3 factorial scheme with two seed sizes (&gt; 15 mm and &lt; 15 mm in diameter) and three substrate types (sand, Plantmax®, and vermiculite), with four replications of 15 seeds. We analyzed germination percentage (G%), number of germinated seeds (NGS), and germination speed index (GSI). Data underwent variance analysis, and means were compared by the Tukey test at 5% probability. Germination began 73 days after sowing. Seed size had no effect on germination. Among substrates, vermiculite showed the best results for GSI, NGS, and G%, followed by sand and then by Plantmax®. Our study provides the first data on factors that affect the germination of this palm tree species. </p>

Clove Extract (Syzygium aromaticum) in Germination and Sanity of Catingueira Seeds (Poincianella pyramidalis (Tul.) L.P. Queiroz)

2020 ◽  
pp. 1-8
Author(s):  
Ediglécia Pereira de Almeida ◽  
Lenita Gonçalves da Costa ◽  
Nathany Alves de Andrade ◽  
Gilvan José Campelo dos Santos ◽  
Maria José de Holanda Leite
Keyword(s):  
Toxic Effect ◽  
Immersion Time ◽  
Germination Percentage ◽  
Syzygium Aromaticum ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Petri Dishes ◽  
Clove Extract ◽  
Health Test ◽  
Germinated Seeds

This research aimed to evaluate the effects of Clove extract (Syzygium aromaticum) on germination and sanity of Catingueira seeds (Poincianella pyramidalis). Five treatments with Clove extract of India were applied at the following concentrations T1 (Control) = 40 mL of sterile H2O, T2= 10 mL extract + 30 mL of sterile H2O, T3= 20 mL extract + 20 mL of sterile H2O, T4= 30 mL extract + 10 mL of sterile H2O and T5= 40 mL of extract. P. pyramidalis seeds were immersed in due concentrations for 30 seconds, then placed in sterilized Petri dishes and taken to isolation chapel. The sanity test lasted seven days, after this period, the associated microorganisms were evaluated in the seeds. For germination tests, the same treatments used for the health test were used. At the end of the experiment the percentage of germinated seeds, the Germination Speed Index (IVG), Mean Germination Time (TMG), Mean Germination Speed (VMG) and the length of part area and root were calculated. Clove extract (Syzygium aromaticum) showed no toxic effect on germination and development of Poincianella pyramidalis. It was indicated the concentration of 25 and 50% of the extract, because it provided a higher germination percentage, IVG, VMG and TMG to seeds of the species Poincianella pyramidalis. Further studies with immersion time of the seeds higher than those of this research are suggested.

scholarly journals Germination of palm seeds under periods of rehydration

2021 ◽  
Vol 27 (4) ◽  
pp. 446-452
Author(s):  
Kássia Barros Ferreira ◽  
Antonio Maricélio Borges de Souza ◽  
Ana Carolina Corrêa Muniz ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Experimental Design ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Drying Process ◽  
Germination Speed ◽  
Favorable Conditions ◽  
Recalcitrant Species ◽  
Seeds Germination ◽  
Germinated Seeds

Abstract Palm seeds are indicative of being recalcitrant species. Seeds germination for many species, is slow and uneven, even in favorable conditions, often due to the resistance of the woody endocarp that makes them impermeable to water. Most species also have the characteristic of not tolerating the drying process at low moisture levels, thus losing their viability. In view of these considerations, the objective was to evaluate the effect of different periods of rehydration on the germinative process of seeds of royal palm, dwarf palm and jeriva. Seeds were homogenized and immersed in water containing 3 times their volume, with daily replacement. The experimental design used was completely randomized, with 5 treatments (0, 3, 6, 9, and 12 days of rehydration), with 4 replications of 25 seeds. The seeds were placed in transparent “gerbox” plastic boxes containing medium vermiculite as substrate and placed in BOD with a photoperiod of 16-hour of light and their respective optimum temperatures for each species. The number of germinated seeds was counted every 2 days until germination stabilized. The variables analyzed were germination percentage, germination speed index (GSI), average germination time (AGT) and distribution of germination over the days. Rehydration for 3 days increased the germination rate of royal palm seeds. For dwarf and jeriva species, rehydration did not influence the analyzed variables. The GSI and AGT were negatively influenced by the rehydration periods for royal palm. Rehydration prolonged the peak of germination of the studied species.

scholarly journals Germination of radish seeds (Raphanus sativus) treated with homeopathic drugs

2021 ◽  
Vol 10 (36) ◽  
pp. 231-232
Author(s):  
Marcelo Felipe Bezerra Donadon ◽  
Euclides Davidson Bueno Romano ◽  
Walkiria Ruiz De Pinho ◽  
Marina Lopes Vieira De Souza ◽  
Pedro Henrique Alcalde Do Nascimento ◽  
...  
Keyword(s):  
Agricultural Research ◽  
Plant Protection ◽  
Point Of View ◽  
Mechanical Equipment ◽  
Distilled Water ◽  
Important Species ◽  
Economic Point ◽  
Arnica Montana ◽  
High Dilutions ◽  
Mother Tincture

The radish is a short cycle crop, since it is harvested at 25-30 days after direct sowing. Under the economic point of view it is an important species, but there are few studies on germination of radish seeds. The objective of this study was to evaluate the effect of high diluted substances on the germination of radish seeds. The trial was conducted at the Agricultural Research Institute of Paraná (IAPAR) in Londrina / Paraná. It was used a pesticide free cultivar named Cometa. The treatments were: Bryonia, hydroalcoholic solution, Arnica montana, Cina and Lupine + Oat, all of them diluted and agitated at 9x; distilled water and agitated distilled water were used as controls. The mother tincture of Lupine + Oat was prepared from plants collected at the experimental station of IAPAR in Londrina. The mother tincture and all treatments were prepared according to the guidelines in the Brazilian Homeopathic Pharmacopeia, Part I [1]. The agitations of the treatments were made by a mechanical equipment, model Denise 10-50 manufactured by Autic. The water was distilled the day before preparing the treatments. The experiment was performed with 300 seeds per treatment. The seeds were placed in gerbox with germitest paper, and 50 gerbox were used per treatment, with six seeds in each gerbox. The germitest papers were moistened with the treatments and the seeds were soaked for 2 hours previously set up the experiment. The substances in high dilutions were agitated 100 times on the machine before soaking the seeds and moistening the germitest paper. The experimental design was entirely randomized and the gerbox were kept at the bench at the Laboratory of Plant Protection of IAPAR. A person not involved in conduct of the experiment coded (blinded) the treatments solutions with a random letter code. The code was kept secret until all measurements and data processing were finished. Seeds were observed daily for germination and counted only those considered germinated. Seeds were considered germinated when the radicle was at least two millimeters length. Arnica montana 9x increased 5.9% the seed germination when compared with distilled water.

Influence of Heat on Seed Germination of Cistus Laurifolius and Cistus Ladanifer

1992 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
L Valbuena ◽  
R Tarrega ◽  
E Luis
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Exposure Time ◽  
Germination Rate ◽  
Heat Exposure ◽  
Cistus Ladanifer ◽  
Different Temperatures ◽  
Petri Dishes ◽  
Germinated Seeds

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.

scholarly journals Filamentous fungi isolated from sand and water of "Bairro Novo" and "Casa Caiada" beaches, Olinda, Pernambuco, Brazil

2008 ◽  
Vol 68 (3) ◽  
pp. 577-582 ◽  
Author(s):  
DNF. Gomes ◽  
MAQ. Cavalcanti ◽  
MJS. Fernandes ◽  
DMM. Lima ◽  
JZO. Passavante
Keyword(s):  
Water Samples ◽  
Water Surface ◽  
High Tide ◽  
Sand Sample ◽  
Distilled Water ◽  
Sabouraud Agar ◽  
Sand Surface ◽  
Heterotrophic Microorganisms ◽  
Petri Dishes ◽  
Dry And Rainy Seasons

Fungi comprehend a heterogeneous group of heterotrophic microorganisms that act as saprobes or parasites or, less frequently, as symbionts living in association with other organisms. They are cosmopolitan and important components of ecosystems. Considering the small number of Brazilian papers on the filamentous mycota in marine environments, and the need to improve knowledge of the diversity of these microfungi in "Casa Caiada" and "Bairro Novo" beaches, Olinda, PE, the aim of this work was to isolate and identify the fungi from sand and water samples of these ecosystems. Thirty two samplings of sand (surface and 20 cm deep) and water (surface and 1 m deep) were carried out during the dry and rainy seasons, in low and high tide. From each sand sample, a suspension was made with 50 g of sand diluted in 90 mL of sterilized distilled water. From each sand suspension and water sample, 0.5 mL was spread, in triplicate, onto Petri dishes containing Sabouraud Agar added of chloramphenicol. The plates were incubated at 28 °C (±2 °C). Fifty seven species were isolated, identified, and classified in 20 genera. Aspergillus and Penicillium were the most frequent genera in both sand and water, with a total of 11 and 19 species, respectively.

scholarly journals Dormancy break in seeds of "quina" (Strychnos pseudoquina A. St.-Hil.)

2011 ◽  
Vol 13 (4) ◽  
pp. 507-511 ◽  
Author(s):  
J.M. Vasconcelos ◽  
M.A. Rodrigues ◽  
S.C. Vasconcelos Filho ◽  
J.F. Sales ◽  
F.G. Silva ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Medicinal Plant ◽  
Gibberellic Acid ◽  
Plant Species ◽  
Germination Percentage ◽  
Boiling Water ◽  
Medicinal Plant Species ◽  
Distilled Water ◽  
Brazilian Cerrado ◽  
Germination Speed

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.

scholarly journals Can tortoises facilitate the germination of plants with dry fruits? A simulation of the effects of Chelonoidis carbonarius (Testudines: Testudinidae) on the germination of Leucaena leucocephala (Fabaceae)

Rodriguésia ◽  
2016 ◽  
Vol 67 (3) ◽  
pp. 661-666
Author(s):  
João Fabrício Mota Rodrigues ◽  
Sâmia Paiva de Oliveira
Keyword(s):  
Seed Germination ◽  
Digestive Tract ◽  
Leucaena Leucocephala ◽  
Acidic Condition ◽  
Natural Environments ◽  
Germination Process ◽  
Germination Speed ◽  
Negative Effect ◽  
Acidic Conditions

Abstract Ingestion of seeds by vertebrates may influence seed germination and dispersion. However the mechanisms responsible by these effects and the importance of turtles in these interactions are still poorly understood. This study aims to evaluate how Chelonoidis carbonarius influences the germination process of Leucaena leucocephala using an experiment conducted at the Federal University of Ceará, Fortaleza-CE, Brazil, that simulates the passage of the seeds of this plant through the digestive tract of C. carbonarius: stomach acidic condition and seeds sown in feces. We demonstrated that sowing the seeds in feces of Chelonoidis carbonarius has a negative effect on the germination of Leucaena leucocephala, decreasing the germination speed index of the seeds, while the acidic conditions have a neutral one. This study is a first attempt to understand how turtles and tortoises may influence the seed germination, and the implications of these findings in natural environments deserve further investigations.

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