Clinical and parasitological assessment in mice treated with highly diluted Atropa belladonna

2021 ◽  
Vol 13 (47) ◽  
pp. 122-124
Author(s):  
Patricia Flora Sandri ◽  
Angela Rigo Portocarrero ◽  
Larissa Ciupa ◽  
Franciele karina Da Veiga ◽  
Gislaine Falkowski Sanchez Falkowski ◽  
...  
Keyword(s):  
Oral Treatment ◽  
Survival Rates ◽  
Public Health Problem ◽  
Clinical Parameters ◽  
Patent Period ◽  
Immunomodulatory Effects ◽  
Atropa Belladonna ◽  
Infection Treatment ◽  
Ad Libitum ◽  
Mother Tincture

Introduction: The infection by Trypanosoma cruzi is a public health problem and there is no effective treatment currently. Immunomodulatory effects of Atropa belladonna may offer benefits1 Objective: To evaluate the effect of A. belladonna in murine infection by T. cruzi. Methodology: The experiment was blind, controlled and randomized by draw. Eighty five Swiss male mice, at 8 weeks of age, were infected with 1400 blood trypomastigotes of T. cruzi Y strain (via IP) and divided into the following groups: without treatment (CI), treated with the mother tincture of A. belladonna (GTM-HN Cristiano), treated with A. belladonna 5cH (G5cH), treated with A. belladonna 6cH (G6cH), treated with A. belladonna 30cH (G30cH). Cereal alcohol 70 ° GL was used for dilutions as well as water in final preparations (Sigma-SP-Brazil). Oral treatment, diluted with water (1mL/100mL water), offered ad libitum 48 hours before infection, available during 16h. After infection, treatment of 56/56h for 16h, until the 9th day of infection2. Parasitological parameters: Curve of parasitemia, total parasitemia (PT), Maximum Peak of Parasites (PMP), Pre-Patent Period (PPP), Patent Period (PP), and Survival. Clinical parameters: water, food, excreta, weight and temperature. Results: G6cH and G30cH groups displayed better survival rates (1.54 and 1.42 times versus IC), and higher curve of parasitemia - G6cH (p = 0.00), G30cH (p = 0.02) – when compared to CI. PMP was lower in GTM (P = 0.01) and G5cH (p = 0.04) groups; PT was lower in GTM (p = 0.01), G5cH (p = 0.05) and G6cH (p = 0.05) groups when compared to CI. There was no difference in PPP and PP parameters in all groups, with a tendency of a higher PPP in G5cH and G6cH groups and lower PPP in G30cHgroup.The mice weight was higher in GTM (

Abstract 038: American Heart Association Mission: Lifeline Breakthrough Series Collaborative Improving the Survival Rates for Out of Hospital Cardiac Arrest by Focusing on Early CPR With an Emphasis on Chest Compressions

2017 ◽  
Vol 10 (suppl_3) ◽  
Author(s):  
Charles R Siegel ◽  
Anjan Chakrabarti ◽  
Lewis Siegel ◽  
Forrest Winslow ◽  
Thomas Hall
Keyword(s):  
Cardiac Arrest ◽  
Quality Improvement ◽  
Performance Measures ◽  
Effective Diffusion ◽  
Survival Rates ◽  
Public Health Problem ◽  
Heart Association ◽  
Performance Measure ◽  
Compression Rate ◽  
Hospital Cardiac Arrest

Introduction: Out-of-hospital cardiac arrest (OHCA) remains a highly morbid public health problem. Despite improving practices and clear guidelines, mortality from this condition remains high at 90%, with survivors often suffering from poor neurologic outcomes. To determine the feasibility of quality improvement collaboratives to narrow gaps between evidence-based practice and patient care for OHCA, we conducted a pilot study of the AHA Resuscitation Collaborative. Methods: Eight emergency medical service agencies participated in the quality improvement collaborative pilot project. We identified several OHCA performance measures to assess the quality of care, guide collaborative activities, and monitor change in performance over time. Over the course of four learning sessions, participants were trained in quality improvement and performance measurement, analyzed performance measure results, and shared successes and challenges. Results: Five remaining agencies underwent the process outlined in Figure 1. Adherence to performance measures, including compression rate compliance (Figure 2), improved over the course of the collaborative. Compression rate compliance in Figure 2 corresponds to the process improvement efforts of the Chesapeake Fire Department with achievement of goals for optimal range of chest compression rate between 100 and 120 compressions per minute during resuscitations. Conclusion: As demonstrated in Virginia, the collaborative approach was an effective framework to improve OHCA care. Improvement in performance measures, the evident commitment of dedicated peers and colleagues, consistent collaboration, and the effective diffusion of best practices all support the continued use of this model.

scholarly journals Survival rate of incoming patients with cardiac arrest at the cardiology infirmary of the emergency department of a public hospital in Greece

2019 ◽  
Vol 5 (2) ◽  
pp. 53
Author(s):  
Styliani Papadopoulou ◽  
Olympia Konstantakopoulou ◽  
Antonia Kalogianni ◽  
Martha Kelesi-Stavropoulou ◽  
Theodore Kapadohos
Keyword(s):  
Emergency Department ◽  
Cardiac Arrest ◽  
Survival Rate ◽  
Emergency Room ◽  
Survival Rates ◽  
Public Health Problem ◽  
Spontaneous Circulation ◽  
Shockable Rhythm ◽  
Discharged Patients ◽  
Hospital Cardiac Arrest

Introduction: Cardiac arrest is an urgent situation that, despite the improved resuscitation capabilities, the survival rate of out-of-hospital cardiac arrest victims remains low.Aim: Τo investigate the survival rate of the incoming patients with cardiac arrest in the cardiology infirmary of the emergency department of a public hospital.Material-Method: The study included 210 patients who were transferred pulseless and breathless at the cardiology infirmary of the emergency department of “Tzaneio” Hospital, Piraeus, during the period April 2017 - November 2018. Data was collected from the National Center of Emergency Dispatch's printed forms, as well as from the patients’ admission book of the emergency department.Results: More than 10% (11.9%) of patients with cardiac arrest returned to spontaneous circulation in the emergency department, of which 16% was discharged. Patients with known cardiac history, (p=0.002), with a shockable rhythm (p<0.001), and especially ventricular fibrillation (p<0.001) upon arrival at the emergency room, and patients who were defibrillated at the ambulance during admission and at the emergency room, were more likely to survive (p<0.001). No statistically significant correlation was found between the factors studied and survival after cardiac arrest, in the group of patients that were discharged.Conclusions: The survival rate of the incoming patients with cardiac arrest at the emergency department of “Tzaneio” Hospital, Piraeus, was low. As for most health systems, this issue constitutes a fairly complex public health problem. Cardiopulmonary resuscitation and corresponding guidelines require further improvement in order for the survival rates of out-of-hospital cardiac arrest patients to increase.

scholarly journals Effect of biotherapy T. cruzi 7x in several therapeutic schemes on experimental infection by Trypanosoma cruzi

2021 ◽  
Vol 10 (36) ◽  
pp. 125-127
Author(s):  
Fabiana Nabarro Ferraz ◽  
Valdir Antônio Gonçalves ◽  
Denise Lessa Aleixo ◽  
Angélica Sayuri Mizutani ◽  
Silvana Marques de Araújo
Keyword(s):  
Water Intake ◽  
Experimental Infection ◽  
Treatment Plan ◽  
Ethics Committee ◽  
Patent Period ◽  
Body Hair ◽  
Important Data ◽  
Swiss Mice ◽  
Food And Water Intake ◽  
Ad Libitum

Background: Biotherapy is used against infectious diseases treatment and prophylaxis and has been investigated by many researchers [1,2]. Aim: Assess the effect of biotherapy 7x T. cruzi on several treatment schemes, upon experimental infection by T. cruzi. Methodology: A blind, controlled and randomized by drawing experiment was performed. Male Swiss mice, four weeks old were utilized. Groups evaluated: IC – Infection Control (treated with water – 9 animals); TBBA7x3days – Treated with biotherapy 7x 3 days before and 3 days after infection (5 animals); TBB7x3days – Treated with 7x biotherapy 3 days before infection (5 animals); TBBAI7x3days – Treated with 7x biotherapy 3 days before infection and after infection indefinitely (6 animals). Animals were inoculated intraperitoneally with 1400 blood trypomastigotes Y strain. Biotherapy: prepared according to Farmacopéia Homeopática Brasileira [3]. Treatment plan: offered ad libitum, in the water (10µL/mL). Parasitological parameters: parasitemia was assessed according Brener’s technique. [4]. Clinical parameters: body hair aspect, edema, movement, diarrhea, body weight, temperature, food and water intake. Ethics: Registration 030/2008 UEM Ethics Committee for Experiments in Animals. Statistical analysis: was performed using the tests Kruskal Wallis and Mann-Whitney testes, significance 5%. Results: The best effect obtained was with the TBBA7x3days, both for clinical and parasitological parameters. It was expressed by lower parasitemia curve (p=0.04) and decrease of patent period tendency, of total parasitemia, of mortality and survival of the animals increase (Table 1). Evolution of parasitemia was distinct for the several treatment schemes. Survival of at least one mouse by treated groups is an extremely important data, since Y strain causes 100% mortality in Swiss mice. TBBAI7x3days group showed begger tendency in raising total parasitemia compared with IC. Although it might have occurred, this group presented 80% mortality rate compared with other groups. Animals from TBBA7x3days also showed better evolution of weight body, temperature, food (p=0.078-10%) and water intake, body hair aspect and edema development. Diarrhea and paralysis were only observed in IC group mice, highlighting the biotherapy use benefits. Conclusions: Best effect was obtained TBBA7x3days, both for clinical and parasitological parameters. It’s possible to speculate that in this regimen, biotherapy was able to modulate, more effectively, the host’s immune system, decreasing the number of parasites.

scholarly journals Long-term outcomes following lateral alveolar ridge augmentation using a collagenated xenogeneic bone block: a monocenter, prospective single-arm clinical study

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Frank Schwarz ◽  
Didem Sahin ◽  
Sara Civale-Schweighöfer ◽  
Jürgen Becker
Keyword(s):  
Survival Rates ◽  
Bone Block ◽  
Clinical Parameters ◽  
Alveolar Ridge ◽  
Alveolar Ridge Augmentation ◽  
Pocket Depth ◽  
Ridge Augmentation ◽  
Probing Pocket Depth ◽  
Implant Placement

Abstract Objectives To assess the long-term clinical outcomes following lateral alveolar ridge augmentation using a collagenated xenogeneic bone block (CXBB) and staged implant placement. Material and methods A total of n = 9 patients (9 implants) were available for the analysis. Each subject had received lateral ridge augmentation using a size-adapted rigidly fixed CXBB and contour augmentation at single-tooth gaps. Implant placement was performed after 24 weeks of submerged healing. Clinical parameters (e.g., bleeding on probing (BOP), probing pocket depth (PD), mucosal recession (MR)) were recorded at 16 to 20 weeks after the cementation of the crown (baseline) and scheduled for 0.5 (visit 1 (V1)), 1.5 (V2), 2.5 (V3), 3.5 (V4), and 4.5 (V5) years after implant loading. Results Changes in clinical parameters commonly remained low throughout the entire observation period. Significant changes to baseline were merely noted for mean BOP scores at V4 (19.14 ± 17.75%; n = 7; P = 0.029) and mean PD scores at V2 (0.78 ± 0.98 mm; n = 9; P = 0.044) and V3 (1.33 ± 1.05 mm; n = 9; P = 0.009), respectively. Conclusion CXBB was associated with high clinical implant success and survival rates on the long-term.

Time Difference of Arrival Based Cancer Tumor Localization Using Magnetic Nano Particles Induced Acoustic Signals

2012 ◽  
Vol 1486 ◽  
Author(s):  
Adi Tsalach ◽  
Idan Steinberg ◽  
Israel Gannot
Keyword(s):  
Survival Rates ◽  
Public Health Problem ◽  
Acoustic Signals ◽  
Time Difference ◽  
Nano Particles ◽  
Tumor Localization ◽  
Major Public Health Problem ◽  
Localization Algorithm ◽  
Time Difference Of Arrival ◽  
Tumor Identification

ABSTRACTCancer is a major public health problem worldwide, especially in developed countries.Early detection of the cancer can greatly increase both survival rates and quality of life for patients. A magnto-acoustic based method had been previously proposed for early tumor detection, in a minimal invasive procedure, using magnetic nanoparticles (MNPs). However, in order to accompany tumor identification with immediate treatment, a complementary tumor localization algorithm was needed. Therefore, our objective was to measure the acoustic signals generated by the MNP conjugated tumor in an optimal multi sensor array and estimate the 3D location of the tumor in real time. A Time Difference of Arrival (TDOA) based localization algorithm was developed, and implemented in computerized simulations on the breast tissue geometry. Tumor localization feasibility and the hyperbolic positioning algorithm performance were evaluated. Overall performance yielded localization with a median Euclidean distance of 2.8mm. Such performance indicates that tumor localization was estimated with high accuracy, and suggests that the combination of magneto-acoustic detection along with a TDOA based localization algorithm can produce an efficient tumor diagnostic system. It enables the detection of tumor presence, as well as the triangulation of its location, and can be further developed into a powerful “image and treat” system.

scholarly journals Synthetic bovine lactoferrin peptide Lfampin kills Entamoeba histolytica trophozoites by necrosis and resolves amoebic intracecal infection in mice

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
César Díaz-Godínez ◽  
Ximena González-Galindo ◽  
Thuluz Meza-Menchaca ◽  
Raúl J. Bobes ◽  
Mireya de la Garza ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Oral Treatment ◽  
Public Health Problem ◽  
Annexin V ◽  
Docking Studies ◽  
Binding Activity ◽  
Bovine Lactoferrin ◽  
Therapeutic Effects ◽  
Intestinal Amoebiasis

AbstractAmoebiasis caused by the protozoan parasite Entamoeba histolytica remains a public health problem in developing countries, making the identification of new anti-amoebic compounds a continuing priority. Previously, we have shown that lactoferrin (Lf) and several Lf-derived peptides exhibit in vitro anti-amoebic activity independently of their iron-binding activity. Here, we evaluated the amoebicidal effect of synthetic Lf-derived peptides Lfcin-B, Lfcin 17-30, and Lfampin, analyzed the mechanism of death induced by the peptides and determined their therapeutic effects on murine intestinal amoebiasis. MTT assays in trophozoite cultures of E. histolytica exposed to each peptide (1–1000 μM) showed that Lfampin is far more amoebicidal than Lfcins. Lfampin killed 80% of trophozoites at doses higher than 100 μM in 24 h, and FACs analysis using Annexin V/propidium iodide showed that death occurred mainly by necrosis. In contrast, Lfcin-B and Lfcin 17-30 appeared to have no significant effect on amoebic viability. FACs and confocal microscopy analysis using FITC-labeled peptides showed that all three peptides are internalized by the amoeba mainly using receptor (PI3K signaling) and actin-dependent pathways but independent of clathrin. Docking studies identified cholesterol in the amoeba’s plasma membrane as a possible target of Lfampin. Oral treatment of intracecally infected mice with the abovementioned peptides at 10 mg/kg for 4 days showed that Lfampin resolved 100% of the cases of intestinal amoebiasis, whereas Lfcin 17-30 and Lfcin-B were effective in resolving infection in 80 and 70% of cases, respectively. These data show that although synthetic bovine Lf-derived peptides exhibit varying amoebicidal potentials in vitro, they do resolve murine intestinal amoebiasis efficiently, suggesting that they may be useful as a therapeutic treatment.

A Toxicity Method with Calcium Cyclamate for Chronic Carcinogenesis Experiments

1972 ◽  
Vol 58 (3) ◽  
pp. 137-142 ◽  
Author(s):  
Bela Toth
Keyword(s):  
Drinking Water ◽  
Experimental Approach ◽  
Survival Rates ◽  
Maximum Tolerated Dose ◽  
Histological Changes ◽  
Swiss Mice ◽  
Body Weights ◽  
Ad Libitum ◽  
Dose Levels

An experimental approach for determining the maximum tolerated dose of chemicals administered continuously or repeatedly ad libitum for the lifespan of animals is described. Five dose levels of calcium cyclamate were given in the drinking water for 35 days to Swiss mice, 8 animals in each group. By taking into account 4 parameters — survival rates, body weights, chemical consumption figures and histological changes — this method enables one to choose with certainty the proper dose, which is prerequisite to designing a chronic carcinogenesis experiment.

Effects of post-weaning stress and feeding management on return to oestrus and reproductive traits during early pregnancy in swine

1976 ◽  
Vol 23 (1) ◽  
pp. 103-110 ◽  
Author(s):  
M. H. Fahmy ◽  
J. J. Dufour
Keyword(s):  
Survival Rates ◽  
Reproductive Traits ◽  
Ovulation Rate ◽  
Embryo Survival ◽  
Feeding Management ◽  
Weaning Stress ◽  
Feeding Regimes ◽  
Ad Libitum ◽  
Breed Crosses ◽  
Interval Weight

SUMMARYA total of 177 sows representing five two-breed crosses were used in a 2×2 factorial experiment to study the effects of post-weaning stress (changing environment and group housing) and feeding regime (flushing by feeding ad libitum until mating) on the weaning-oestrus interval and other reproductive traits up to 30 days of pregnancy.The group which was stressed had about 10% (P<0·05) fewer reproductive failures than those not stressed, while little difference was found between the two feeding regimes. About 61% of the flushed sows returned to oestrus within 7 days after weaning, 9% higher than for those not flushed. The stress treatment had little effect on the weaning-oestrus interval. Neither treatment affected ovarian and follicular-fluid weights, percentage of follicles of different sizes, ovulation rate nor number of viable embryos. The flushed group, however, had 9% lower embryo survival than the group not flushed. A positive relation was found between the number of days on ad libitum feeding and ovulation rate and embryonic mortality.Differences among breeds were significant for all the traits, except weaning-oestrus interval, weight of follicular fluids, percentage of large follicles, ovulation and survival rates. The weaning-oestrus interval was not significantly correlated with any of the reproductive traits studied.

scholarly journals Medidas De Prevención De Infecciones Y Colonizaciones Nosocomiales Por Enterococcus Faecium Resistente A Vancomicina

2021 ◽  
Vol 17 (25) ◽  
pp. 193
Author(s):  
Sandra Alvarez Hidalgo ◽  
Ruth Magdalena Gallegos Torres ◽  
Juan Campos Guillén ◽  
Maribel Liñan Fernandez ◽  
Aide Teran Alcocer ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Prevention And Control ◽  
English Language ◽  
Positive Impact ◽  
Public Health Problem ◽  
Epidemiological Surveillance ◽  
Global Public Health ◽  
Infection Treatment ◽  
Health Care Associated Infections ◽  
And Control

Las Infecciones Asociadas a la Atención de la Salud (IAAS) son un problema de salud pública global, causado por microorganismos oportunistas, dentro de los cuales los Enterococcus faecium resistentes a vancomicina (ERV) son una de las tres principales especies de bacterias responsables. La colonización intestinal por ERV es considerada requisito y factor de riesgo para el desarrollo de estas infecciones. La presencia de ERV se ha asociado a multi-terapia antimicrobiana, largas estancias hospitalarias, inmunosupresión y enfermedades graves. Se ha propuesto que la diseminación intrahospitalaria entre pacientes, por el personal de salud o vía fómites, es el mecanismo de transmisión en infecciones y brotes hospitalarios. Éste es un artículo de revisión que resume las publicaciones en habla inglesa de la última década, acerca de las principales recomendaciones de prevención y control de brotes por ERV. Las medidas para la prevención y contención de la colonización e infecciones por ERV se clasificaron en: 1) medidas de vigilancia epidemiológica y detección temprana; 2) prevención de la colonización y eliminación de reservorios; 3) Manejo de pacientes colonizados y/o infectados; y 4) recomendaciones para el uso razonados de antimicrobianos y tratamiento empírico inicial de las infecciones por ERV. El impacto positivo de la implementación de este tipo de medidas ha sido demostrado por distintos autores. Health Care Associated Infections (HAI) are a global public health problem caused by opportunistic microorganisms, among which vancomycin resistant Enterococcus faecium (VRE) are considered one of the three main responsible bacteria. The colonic colonization by VRE is considered a requirement as well as risk factor for the development of these infections. The presence of VRE has been associated with multiple antimicrobial therapy, prolonged hospitalization, immunosuppression, or serious diseases. Intrahospital dissemination between patients, through healthcare workers or via fomites, has been described as the transmission mechanism in endemic infections and hospital outbreaks. This is a review article in Spanish that compiles the English-language publications of the last decade, about the main recommendations for the prevention and control of VRE outbreaks. Recommendations for prevention and control of VRE colonization and infection are classified in: 1) epidemiological surveillance and early detection measures; 2) prevention of colonization and reservoirs elimination; 3) management of colonized and / or infected patients; and 4) recommendations for rational use of antimicrobials and initial empirical VRE infection treatment. The positive impact of the implementation of this type of measures has been demonstrated by different authors.

scholarly journals Menthol for the Treatment of Erectile Dysfunction: Generation and Technology Export

2021 ◽  
pp. 5747-5763
Author(s):  
Rafael Leonne Cruz De Jesus
Keyword(s):  
Erectile Dysfunction ◽  
Trp Channels ◽  
Technological Development ◽  
Oral Treatment ◽  
Public Health Problem ◽  
New Drugs ◽  
Trpm8 Channel ◽  
Innovation Potential ◽  
Clinical Complications ◽  
Novel Target

Erectile dysfunction (ED) is considered a public health problem with numerous risk factors, such as hypertension. Besides the existing oral treatment for ED, there are individuals that do not respond to or present numerous side effects to this therapy. Changes in the TRP channels can lead to several clinical complications, such as hypertension. More specifically, the cooling-sensing TRPM8 channel could be a novel target for the development of new drugs. In this line, menthol, a natural product TRPM8 channel agonist may be a molecule for use to treat erectile dysfunction. Then, we conducted a patent review to evaluate the application of menthol focusing on the treatment of erectile dysfunction. The search was conducted on Espacenet® associating A61K31/045 and A61K36/534 codes or using keywords, “erectile dysfunction AND menthol”. We analyzed 1,331 patents, which fourteen patents were found with the use of menthol in the genitourinary system. Although the patents had menthol in their formulations, none went directly to the development of pharmaceutical applications to treat erectile dysfunction. Taking these data into account, the use of menthol in the treatment of erectile dysfunction has been underexplored and is an opportunity for research and technological development based on a high innovation potential.

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