Investigations and Concerns about the Fate of Transgenic DNA and Protein in Livestock

2021 ◽  
Author(s):  
Ahmet Aykaç ◽  
İzel Ok
Keyword(s):  
Zinc Oxide Nanoparticles ◽  
Wound Dressing ◽  
Mesoporous Silica Nanoparticles ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Release Time ◽  
Wound Healing Process ◽  
Zno Nps ◽  
And Control ◽  
Scanning Electron

Hydrogels are biocompatible and swollen materials that have been used as a wound dressing for years. Among them, chitosan-based hydrogels have become popular in the wound healing process owing to their low toxic, biocompatible, biodegradable, antibacterial properties. Chitosan (CS) has been used either as a pure form or incorporated with polymers or nanoparticles to increase antimicrobial activity and stability. In this context, zinc oxide nanoparticles (ZnO NPs) have been used to enhance antibacterial activity and mesoporous silica nanoparticles (MSN) have been employed to develop mechanical strength and control of drug release time. In this study, we report the synthesis and fully characterizations of ZnO NPs, MSN and the hydrogel by using dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). We have also prepared and characterized chitosan-based hydrogels functionalized by MSNs and ZnO NPs.

scholarly journals Starch/hyaluronic acid/sorbitol composite films as potential wound dressing materials that protect against P. Aeruginosa

2019 ◽  
Vol 10 (SPL1) ◽  
Author(s):  
R. M. Zaki ◽  
K. Ramasamy ◽  
R. K. Mishra ◽  
A. B. A. Majeed ◽  
S. M. Lim
Keyword(s):  
Hyaluronic Acid ◽  
Bacterial Infection ◽  
Wound Dressing ◽  
Healing Process ◽  
Composite Films ◽  
Antibacterial Properties ◽  
Wound Healing Process ◽  
Protection Against Infection ◽  
Dressing Materials ◽  
Wound Dressing Materials

Bacterial infection is one of the key challenges to the wound healing process. Ideal wound dressing materials should, therefore, be able to inhibit bacterial infection. The present study evaluated the antibacterial properties of composite films for use as wound dressing materials. The composite films, which were made up of starch, sodium hyaluronic acid (SHA), and sorbitol, were prepared at varying concentrations of hyaluronic acid (25-100mg) by solvent casting method. The composite films were tested against gram-negative Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhimurium as well as gram-positive Staphylococcus aureus for 24 hours by using the disc diffusion assay. Chloramphenicol and ciprofloxacin were included as positive controls. Amongst the tested pathogens, composite films with SHA > 25mg inhibited only P. aeruginosa. It is noteworthy that the antibacterial effect of composite films with SHA > 50mg were comparable to those of chloramphenicol and ciprofloxacin. The present findings implied the possible incorporation of starch/ hyaluronic acid/ sorbitol composite films as part of wound dressing for protection against infection caused by P. aeruginosa.

A Comprehensive Review On Alginate As Wound Dressing Biomaterial

2020 ◽  
Vol 04 ◽  
Author(s):  
Sourav Mohanto ◽  
Prithviraj Chakraborty ◽  
Chidambaram Soundra Pandian ◽  
Shubhradeep Manna ◽  
Joni Dutta
Keyword(s):  
Therapeutic Efficacy ◽  
Wound Dressing ◽  
Chronic Wound ◽  
Healing Process ◽  
Wound Healing Process ◽  
Detailed Knowledge ◽  
Pathophysiological Mechanisms ◽  
Moist Environment ◽  
Bioactive Agents ◽  
Mechanical Nature

Background: The design and characteristics of alginate biomaterial have a significant role in wound dressing and tissue regeneration. The ideal biomaterial for wound dressing must have biodegradable, biocompatible, non-inflammatory, non-toxic. Objective: Wound dressing should promote the re-epithelization and protect the wound for further infection by creating a moist environment. The physical and mechanical nature of the alginate biopolymer has the potential to influence the pathophysiological mechanisms in the chronic wound actively. The application of this biomaterial provides an ampule advantage to synthetic polymers. Method: The wound healing process is a critical step involved in different phases. The presence of cross-linkers, polymers along with alginate lead to a decrease in the mechanical property of dressing. Hence the effective material choosing along with alginate is a very critical decision. The therapeutic efficacy of the alginate dressing system (film, hydrogel, wafer, etc.) influenced by the incorporation of different materials such as bioactive agents, nanoparticles, cross-linker, nature of the excipients, etc. Results: The ion exchange occurring between dressing and exudates resulted in the formation of gel, due to the glucuronic acid presence in alginate. This gel absorbs moisture and maintains an appropriate moist environment and actively influence the pathophysiological mechanisms in the chronic wound. Conclusion: This review gives a detailed knowledge of the researchers to work with alginate and provides knowledge about the incorporation of the appropriate material and their therapeutic efficacy in wound dressing.

scholarly journals Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2554
Author(s):  
Marek Konop ◽  
Anna K. Laskowska ◽  
Mateusz Rybka ◽  
Ewa Kłodzińska ◽  
Dorota Sulejczak ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

Lichen Based Synthesis of Zinc Oxide Nanoparticles and Evaluation of its Neurotoxic Effects on Human Neuroblastoma Cells

2019 ◽  
Vol 59 ◽  
pp. 15-24
Author(s):  
Fatih Doğan Koca ◽  
Gökhan Ünal ◽  
Mehmet Gökhan Halici
Keyword(s):  
Hydrogen Peroxide ◽  
Zinc Oxide Nanoparticles ◽  
Neuroblastoma Cells ◽  
Oxide Nanoparticles ◽  
Human Neuroblastoma Cells ◽  
Zno Nps ◽  
Human Neuroblastoma ◽  
Neurotoxic Effects ◽  
Higher Doses ◽  
Scanning Electron

Nanoparticles, smaller than 100 nm are synthesized by chemical and physical methods. Biological synthesis of nanoparticles is very popular in science recently. The aim of the study is green synthesis of zinc oxide nanoparticles (ZnO NPs) using the lichen extract (Ramalina fraxinea) and investigating the cytotoxic effects of ZnO NPs on human neuroblastoma cells (SHSY-5Y). Despite the widespread use of ZnO NPs, a limited number of studies have investigated the neurobiological effects of ZnO NP. Therefore, we tested the neurotoxic effect of green synthesized ZnO NPs administration and its neuroprotective effect against hydrogen peroxide-induced cell damage on SH-SY5Y human neuroblastoma cell line. The absorbance peak of the ZnO NPs was detected by UV–visible spectroscopy (UV-Vis) at 330 nm. The average diameter of ZnO NPs was measured as about 21 nm by Scanning Electron Microscope (SEM) and Field Emission Scanning Electron Microscope (FE-SEM) images. According to X-ray Diffraction (XRD) diagram, ZnO NPs were hexagonal in structure. The peaks observed in the Fourier Transform Infrared (FT-IR) test showed functional groups in the structure of the nanoparticles. According to our results, ZnO NPs may have beneficial effects at the low concentrations while neurotoxic effects at the higher doses in SH-SY5Y. In addition, we indicate that hydrogen peroxide-induced cell death could not reverse by ZnO NPs and its higher doses potentiated the neurodegenerative effect of hydrogen peroxide. In conclusion, here we report that ZnO NPs, widely used in various products, may have beneficial or harmful effects in a dose-dependent manner and play a role in neuropsychiatric disease, especially neurodegenerative diseases. This is the first study dealing with neurotoxicity on SHSY-5Y of Ramalina fraxinea extract based ZnO NPs.

scholarly journals Effect of Carica papaya Extract toward Incised Wound Healing Process in Mice (Mus musculus) Clinically and Histologically

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Rachmi Fanani Hakim ◽  
Fakhrurrazi ◽  
Dinni
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Carica Papaya ◽  
Healing Process ◽  
Antibacterial Properties ◽  
The Body ◽  
Wound Healing Process ◽  
Protective Function ◽  
Incised Wound ◽  
Anterior Teeth

Wound healing entails a sequence of complex biological processes, which is a protective function of the body that focuses on a quick recovery. Reducing wound healing time is crucial in a wound as it lowers the chance of infection and decreases complications and costs. Papaya extract was obtained by a maceration method. It contains papain enzyme, flavonoid, saponin, and alkaloid, which act as an anti-inflammatory, astringent substance, vasodilator, antioxidant, analgesic, with antifungal, and antibacterial properties, and increase the collagen synthesis. This study aimed to assess the effect of Carica papaya extract application toward incised wound healing process in mice (Mus musculus) clinically and histologically. This experimental laboratory study was performed using 24 mice weighing between 30 and 40 grams and 12 and 14 weeks of age as experimental animals. Each group was incised along 5 mm at the labial gingiva under both of mandible anterior teeth with a depth reaching alveolar bone. Papaya extract was applied topically in the morning and evening for 14 days. The clinical result was obtained by assessing the length of wound closure measured every day for 14 days. Based on the statistic test result, it showed that the papaya extract has a significant effect (p≤0.05) toward the healing process of an incised oral wound in mice. Histologically at day 14, 75% concentration papaya extract showed perfect epithelial layer and fibrillation.

Studies of mechanical properties of electrospun zein/poly(ɛ-caprolactone) composites and antibacterial properties against Listeria monocytogenes strains of zein/poly(ɛ-caprolactone)/ poly(ɛ-lysine) films

2017 ◽  
Vol 88 (24) ◽  
pp. 2800-2809 ◽  
Author(s):  
Xiyu Hu ◽  
Liang liang Yang ◽  
Kaijun Wang ◽  
Yanli Zixuan Wei ◽  
Hongwu Deng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Listeria Monocytogenes ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Mechanical Analysis ◽  
Hydrophilic Surface ◽  
Packing Materials ◽  
Antimicrobial Films ◽  
Scanning Electron

Ultra-fine fibers of zein/poly(ɛ-caprolactone) (PCL) composites were prepared by oriented electrospun technology to enhance the toughness and ductility of zein. The mechanical, morphological and thermal properties of fibrous films of zein/PCL were studied by texture analysis and nanoindentation tests, scanning electron microscopy and dynamic mechanical analysis. Wettability of fibrous films was also measured and discussed. The mechanical properties of these fibrous films were significantly improved with the addition of PCL. Research on the antibacterial activities of poly(ɛ-lysine) (EPL) loaded films of zein/PCL/poly(ɛ-lysine) shows that the films could inhibit the growth of Listeria monocytogenes. Films of zein/PCL blend systems with a hydrophilic surface do not dissolve in water and have excellent toughness and ductility. This is very favorable for applications in biodegradable materials, food packing materials, facial masks and as antimicrobial films for wound dressing.

scholarly journals Assessing Nursing Practices Regarding Standard Approaches on Post-Operative Wound Care Dressing

2020 ◽  
Vol 24 (2) ◽  
pp. 179-186
Author(s):  
SAIMA SALEEM ◽  
MUHAMMAD HUSSAIN ◽  
MUHAMMAD AFZAL ◽  
SYED AMIR GILANI
Keyword(s):  
Nursing Education ◽  
Wound Dressing ◽  
Wound Care ◽  
Healing Process ◽  
Wound Healing Process ◽  
Cross Sectional ◽  
Before And After ◽  
The Mean ◽  
Female Nurses ◽  
Operative Wound

Background and Objective:  Post-operative delay wound dressing causes a wound contamination or delay wound healing process. Wound leakage and smell produce acute wounds changed into chronic wound conditions. This study assessed the nursing practices regarding standard approaches used for post-operative wound dressing and its care. Materials and Methods:  A cross-sectional observational study designed with study population n = 150 in female nurses of two intensive care units and three surgical wards units: I-II-III. Data was collectedusing an adopted, observation checklist. Reliability tested by Cronbach alpha, 0.92. The mean scorewas categorized following rules, less than (1.5) was considered low (L), from (1.5-2.5) considered moderate (M), and greater than (>2.5) was considered high (H). Results:  The results indicated that 55.3% of nurses had 32 – 38 of age and 48.7% were BS nursing education. According to the mean score majority of nurses had medium (M) and low (L) wound dressing practices observed. Plaster not present (52%) during dressing. 73.3% of nurses had not ensured privacy. The commonpractices were found, no handwashing before and after wound dressing. Conclusion:  The majority of the nurses were not following the standardized wound dressing checklist. According to the study, findings recommended the education sessions, seminars, frequent departmental observation, and psychomotor simulation practices that need to be discussed with clinical professionals.

scholarly journals Intelligent wound dressing for diagnostic & therapeutic applications - critical to wound infection

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Zahid Mahmood ◽  
Anne-Marie Salisbury ◽  
Rui Chen ◽  
Stephen Rimmer ◽  
Steven Percival
Keyword(s):  
Wound Dressing ◽  
Antimicrobial Agents ◽  
Detection System ◽  
Healing Process ◽  
Wound Dressings ◽  
Core Material ◽  
Wound Healing Process ◽  
Polymer Complex ◽  
Triggered Release ◽  
Antibiofilm Agents

A medical device comprising of biomaterials responsive to biochemical stimuli: channel for indicating the infective states of wounds and ensuring delivery of smart antimicrobial and antibiofilm agents to promote tissue regeneration and healing. The importance of providing diagnostic wound dressings that can inform healthcare professionals on the state of infection within wounds but also provide some of the treatment required in response to at risk or infected wounds is of key interest. The aim is to investigate an innovative proof of concept diagnostic and detection system, an intelligent hydrogel wound dressing that responds to specific biochemical stimuli in wounds (MMPs and pH) enabling the selective and triggered release of antibiofilm and antimicrobial agents (‘Detect and Treat’)to the trauma site. The dressing is made of a sterile alginate core material covered in a biocompatible dry or hydrated peptide-polymer-complex film and may include a fluorescent dye which upon release during the wound healing process indicates when a change in dressing is necessary. Efficacy studies of the hydrogel dressing were performed within a drip-flow bioreactor in which regression of Pseudomonas aeruginosa biofilm was observed. A 5-log reduction in biofilm was observed in comparison to an untreated control biofilm. The hydrogel dressing indicated a clear response when in contact with biofilms produced only by pathogenic strains of bacteria when analysed. This further confirmed the adequate release and function of the antimicrobial and antibiofilm agents within the peptide-polymer-complex formulation of the hydrogel wound dressing.

scholarly journals Collagen-Based Nanofibers for Skin Regeneration and Wound Dressing Applications

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4368
Author(s):  
Zintle Mbese ◽  
Sibusiso Alven ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Cellular Proliferation ◽  
Healing Process ◽  
Skin Regeneration ◽  
Wound Dressings ◽  
Wound Management ◽  
Major Constituent ◽  
Wound Healing Process ◽  
Frequent Change

Skin regeneration after an injury is very vital, but this process can be impeded by several factors. Regenerative medicine is a developing biomedical field with the potential to decrease the need for an organ transplant. Wound management is challenging, particularly for chronic injuries, despite the availability of various types of wound dressing scaffolds in the market. Some of the wound dressings that are in clinical practice have various drawbacks such as poor antibacterial and antioxidant efficacy, poor mechanical properties, inability to absorb excess wound exudates, require frequent change of dressing and fails to offer a suitable moist environment to accelerate the wound healing process. Collagen is a biopolymer and a major constituent of the extracellular matrix (ECM), making it an interesting polymer for the development of wound dressings. Collagen-based nanofibers have demonstrated interesting properties that are advantageous both in the arena of skin regeneration and wound dressings, such as low antigenicity, good biocompatibility, hemostatic properties, capability to promote cellular proliferation and adhesion, and non-toxicity. Hence, this review will discuss the outcomes of collagen-based nanofibers reported from the series of preclinical trials of skin regeneration and wound healing.

scholarly journals Gambaran Pengetahuan Perawat Tentang Perawatan Luka Modern Dressing di Satu Rumah Sakit Swasta di Indonesia Barat

2019 ◽  
Vol 1 (2) ◽  
pp. 77-86
Author(s):  
Anggiriani Meilin Br. Sidabutar ◽  
Rika Anggelina Patty ◽  
Sonta Simanjuntak ◽  
Lia Kartika ◽  
Sumiaty Aiba
Keyword(s):  
Wound Dressing ◽  
Wound Care ◽  
Rapid Development ◽  
Healing Process ◽  
Univariate Analysis ◽  
Wound Healing Process ◽  
Assessment And Treatment ◽  
Quantitative Descriptive ◽  
June July ◽  
Nurses Knowledge

Currently, the wound care technique is experiencing rapid development, especially modern wound dressing treatments that maintain the moist dressing principle. Wound care is one of the nurses' skills that should be known by all nurses. Data shows that five out of eight nurses are not aware of modern wound dressing techniques. This condition was becoming a cause for concern because inadequate knowledge can make a non-comprehensive wound assessment and treatment that can slow down or even hinder the client's wound healing process. This study aimed to determine the description of nurses' knowledge of modern dressing wound care. Quantitative descriptive methods with total sampling techniques were conducted on 50 respondents who worked in the inpatient rooms on 5th, 6th, and 7th floor in June-July 2019. Univariate analysis was used to obtain an overview of nurses' knowledge of modern dressing wound care techniques. Most of the nurses' knowledge about modern wound dressing care has sufficient knowledge, namely 33 people (66%). Special training and seminars on modern dressing need to be carried out to increase knowledge and ensure the authority of nurses in wound care. Further researchers are advised to identify factors that influence nurses' knowledge of modern dressing wound care.                                                        

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