Physical and Chemical methods of extraction of Bioactive Molecules from Lepidium sativum Linn. and Antioxidant Activity-based screening and selection of extracts-Probable Phytochemical, Chromatography and mass spectroscopy analysis-based correlates

2021 ◽  
pp. 3082-3092
Author(s):  
Rajasekaran R. ◽  
Suresh P. K.
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Molecules ◽  
Superior Performance ◽  
Lepidium Sativum ◽  
Total Phenolic ◽  
Published Data ◽  
Meoh Extract ◽  
Assisted Extraction ◽  
Physical And Chemical ◽  
Lepidium Sativum L

Identification and isolation of active principles from Lepidium sativum L. garden cress seeds and their chemical/biological characterization would aid in drug development (templates for synthesizing derivatives or as a value-added product). Initially, this study dealt with the phytochemical, qualitative and quantitative analysis for phenolic and flavonoid compounds. Subsequently, different physical and chemical extraction techniques -Simple Crude extraction (CRU), Ultra Sonication-Assisted Extraction (UAE), Microwave-Assisted Extraction (MAE) and classical Soxhlet extraction were adopted to select the best Lepidium sativum L. seed extract based on their activity in certain classical, in vitro antioxidant assay systems. The selected best extract was further analyzed for characterization using UPLC, GC-MS. CRU MeOH extracts showed a higher Total phenolic content of 4464.1±349.7mg GAE/100g. The UAE method showed a higher total flavonoid content of 1520.6±182.2mg QUE/100g. CRU-MeOH showed good antioxidant activity based on the free radical scavenging DPPH assay (IC50: 50.61µg/mL). A 100µg/mL concentration of CRU-MeOH was found to be 217.82±12.82 FRAP value. At 400 µg/mL, the NO scavenging assay was reported to be 62.11±4.84%. The present study indicates that extracts from the polar solvents shows better antioxidant scavenging potential than that of the non-polar extracts. Based on the relatively superior performance of the CRU-MeOH extract, UPLC-PDA data was generated. The validity of our chromatography conditions was shown by the presence of quercetin at a retention time 8.555 (validated by our internal standard), apart from the presence of this important bioactive molecule in our extract. As another strand of evidence for the presence of important bioactive molecules, p-coumaric acid, and ferulic acid were also present in our extracts, following correlation of their UPLC-PDA data with published findings. Also, GC-MS analysis of the CRU MeOH extract showed that 24 compounds (8 major and 16 minor peaks). One of the compounds detected (cis-vaccenic acid) is in consonance with published data. The presence of bioactive components, with known antioxidant and cell death potential, validates our experimental flow for the development and/or refinement of crude extract-based drugs. However, the variability perforce warrants an inter-laboratory harmonization of protocols for making meaningful comparisons.

scholarly journals Phytotoxicity of Tires Evaluated in Simulated Conditions

Environments ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Markéta Šourková ◽  
Dana Adamcová ◽  
Jan Winkler ◽  
Magdalena Daria Vaverková
Keyword(s):  
Higher Plants ◽  
Sinapis Alba ◽  
Lepidium Sativum ◽  
Root Growth Inhibition ◽  
Chemical Parameters ◽  
Sinapis Alba L ◽  
Test Kit ◽  
Physical And Chemical ◽  
Aqueous Leachate ◽  
Lepidium Sativum L

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.

scholarly journals Phytochemicals and Antioxidant Activity in Oat-Buckwheat Dough and Cookies with Added Spices or Herbs

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2267
Author(s):  
Małgorzata Starowicz ◽  
Saruhan Arpaci ◽  
Joanna Topolska ◽  
Małgorzata Wronkowska
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Weight Basis ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Wide Range ◽  
Confectionery Products ◽  
Ferric Reducing Antioxidant Power ◽  
Phytochemical Contents

The aim of this study was to determine the phytochemicals and antioxidant activity in oat-buckwheat doughs and cookies with the addition of ten selected spices or herbs (2 g/100 g flours weight basis). The used spices and herbs, as was expected, showed a wide range of bioactive molecules, namely phenolic acids and flavonoids, and they are a rich source of components with antioxidant potential. All analysed oat-buckwheat dough showed higher antioxidant activity potential and higher total phenolic content (TPC) compared to cookies. The highest TPC was found in clove, both dough and cookies, with its addition showing the highest ferric reducing antioxidant power. Generally, cookies with the addition of spice/herbs showed higher phytochemical contents and antioxidant activity compared to oat-buckwheat cookies without the condiment. The technology of obtaining confectionery products, like oat-buckwheat cookies, that will favor the protection of bioactive compounds should still be improved.

scholarly journals Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 582
Author(s):  
Inês Mansinhos ◽  
Sandra Gonçalves ◽  
Raquel Rodríguez-Solana ◽  
José Luis Ordóñez-Díaz ◽  
José Manuel Moreno-Rojas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Industrial Applications ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasonic Assisted

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Comparison of Different Extraction Methods for the Determination of the Antioxidant and Antifungal Activity of Cynara Scolymus and C. Cardunculus Extracts and Infusions

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Eleni Kollia ◽  
Panagiota Markaki ◽  
Panagiotis Zoumpoulakis ◽  
Charalampos Proestos
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Globe Artichoke ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Scavenging Capacity ◽  
Maximum Inhibition

Extracts and infusions of wild artichoke ( Cynara cardunculus L.) and globe artichoke ( C. scolymus L.) (heads, bracts and stems) were examined for their total phenolic content (TPC) and their antioxidant activity after performing Classical Extraction (CE) and Ultrasound-Assisted Extraction (UAE). UAE proved to be more effective, since extracts exhibited higher antioxidant activity and TPC values than CE extracts and infusions. Moreover C. cardunculus heads extract using UAE, displayed the maximum TPC values (1.57 mg gallic acid equivalents (GAE) g−1 fresh weight (fw)), the highest DPPH• scavenging activity (IC50; 0.91mg mL−1) and the highest ABTS•+ radical scavenging capacity (2.08 mg Trolox Equivalents (TE) g−1 fw). Moreover, the effect of different concentrations of C. cardunculus head extracts (showing the highest TPC and antioxidant activity) on Aspergillus parasiticus growth was estimated in AFPA medium. The maximum inhibition was found to be ~42.1% in comparison with the control.

scholarly journals Optimization of Ultrasound Assisted Extraction of Functional Ingredients from Stevia Rebaudiana Bertoni Leaves

2015 ◽  
Vol 29 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Jana Šic Žlabur ◽  
Sandra Voća ◽  
Nadica Dobričević ◽  
Mladen Brnčić ◽  
Filip Dujmić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Procedure ◽  
Total Phenolic ◽  
Steviol Glycosides ◽  
Total Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Probe Diameter ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Abstract The aim of the present study was to reveal an effective extraction procedure for maximization of the yield of steviol glycosides and total phenolic compounds as well as antioxidant activity in stevia extracts. Ultrasound assisted extraction was compared with conventional solvent extraction. The examined solvents were water (100°C/24 h) and 70% ethanol (at 70°C for 30 min). Qualitative and quantitative analyses of steviol glycosides in the extracts obtained were performed using high performance liquid chromatography. Total phenolic compounds, flavonoids, and radical scavenging capacity by 2, 2-azino-di-3-ethylbenzothialozine- sulphonic acid) assay were also determined. The highest content of steviol glycosides, total phenolic compounds, and flavonoids in stevia extracts were obtained when ultrasound assisted extraction was used. The antioxidant activity of the extracts was correlated with the total amount of phenolic compounds. The results indicated that the examined sonication parameters represented as the probe diameter (7 and 22 mm) and treatment time (2, 4, 6, 8, and 10 min) significantly contributed to the yield of steviol glycosides, total phenolic compounds, and flavonoids. The optimum conditions for the maximum yield of steviol glycosides, total phenolic compounds, and flavonoids were as follows: extraction time 10 min, probe diameter 22 mm, and temperature 81.2°C.

scholarly journals Ultrasound and Microwave Assisted Extraction of Opuntia Fruit Peels Biocompounds: Optimization and Comparison Using RSM-CCD

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3618 ◽  
Author(s):  
Bruno Melgar ◽  
Maria Inês Dias ◽  
Lillian Barros ◽  
Isabel C.F.R. Ferreira ◽  
Antonio D. Rodriguez-Lopez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Bioactive Molecules ◽  
Specific Response ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ic50 Values ◽  
Effective Models

Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) of bioactive compounds, peels from Opuntia engelmannii cultivar (cv.) Valencia were optimized by response surface methodology. Randomized extraction runs were performed for each of the technologies employed in order to build effective models with maximum (bioactive molecules content and yield) and minimum (antioxidant activity) responses. A 5-level, 4-factor central composite design was used to obtain target responses as a function of extraction time (t), solid to liquid ratio (S/L), methanol concentration (metOH), and temperature (T). Specific response optimization for each technology was analyzed, discussed, and general optimization from all the responses together was also gather. The optimum values for each factor were: t = 2.5 and 1.4 min, S/L = 5 and 5 g/L, metOH = 34.6 and 0% of methanol and T = 30 and 36.6 °C, achieving maximum responses of 201.6 and 132.9 mg of betalains/g, 13.9 and 8.0 mg of phenolic acids/g, 2.4 and 1.5 mg of flavonoids/g, 71.8% and 79.1% of extractable solid and IC50 values for the antioxidant activity of 2.9 and 3.6, for UAE and MAE, respectively. The present study suggested UAE as the best extraction system, in order to maximize recovery of bioactive compounds with a high antioxidant activity.

On the Expression of Several Lhc Genes in Garden Cress (Lepidium sativum L.)

1994 ◽  
Vol 49 (11-12) ◽  
pp. 802-810 ◽  
Author(s):  
Harald Brunner ◽  
Wolfhart Rüdiger
Keyword(s):  
Lepidium Sativum ◽  
Published Data ◽  
Coding Region ◽  
Chain Reaction ◽  
Transcript Levels ◽  
Coding Regions ◽  
B1 Gene ◽  
Polymerase Chain ◽  
Lhc Genes ◽  
Lepidium Sativum L

The polymerase chain reaction was used to prepare gene-specific probes for several Lhc genes coding for chlorophyll a/b-binding proteins of cress (Lepidium sativum L.). Due to the presence of about 150 basepairs of the coding region, the isolated clones could be attributed to Lhc a3 (1 clone), Lhc b1 (5 clones), Lhc b2 (1 clone) and Lhc b3 (1 clone) genes. Probes prepared from the 3′ non-coding regions of the clones did not cross-hybridize; they were specific for 3 different Lhc b1 transcripts and one each of Lhc b2, Lhc b3 and Lhc a 3 transcripts. The transcript levels were higher in leaves than in cotyledons of light-grown seedlings; they decreased significantly in cotyledons from week 1 to week 4. The levels of 2 Lhc b1 transcripts (detected with probes cd 1 and cd 2) changed from 1 week old cotyledons (30% c d l, 28% cd 2) to 3 months old leaves (14% c d l), 44% cd 2), stems (11% c d l, 56% cd 2) and fruits (15% cd 1, 62% cd 2, all values percent of total transcripts), whereas transcript levels of another Lhc b1 gene (detected with probe cd 3) and of a Lhc a 3 gene remained nearly constant. The level of Lhc b2 and Lhc b3 transcripts were 1 - 2 orders of magnitude smaller than those of the other Lhc transcripts. The data obtained with cress plants are compared with published data from other plants.

scholarly journals Physical and Chemical Characteristics of Pomegranates

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1200-1203 ◽  
Author(s):  
Pavlina D. Drogoudi ◽  
Constantinos Tsipouridis ◽  
Zisis Michailidis
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Total Phenolic ◽  
Chemical Characteristics ◽  
Total Anthocyanin ◽  
Physical And Chemical Characteristics ◽  
Red Color ◽  
Hue Angle ◽  
Leaf Dimensions ◽  
Physical And Chemical

Twenty pomegranate (Punica granatum L.) accessions were collected from different regions in northern Greece and evaluated under uniform conditions for leaf dimensions, frost resistance, and fruit physical and chemical characteristics, such as the juice antioxidant activity [ascorbate equivalent antioxidant activity (AEAC)], using the radical DPPH, ascorbic acid, total phenolic and total anthocyanin contents. Considerable variation in the characteristics studied was found and valuable pomological traits were exhibited. Cluster analysis produced cases of homonymy between some accessions. Principal component analyses showed that the component explaining the greatest variability positively correlated with percent juice, fruit chroma, AEAC, total anthocyanins, and soluble solids content (SSC), but negatively correlated with fruit fresh weight (FW), fruit and seed hue angle (less red color), total acidity, and leaf dimensions. Fruit size was positively correlated with acidity, while acidity was negatively correlated with SSC. Skin thickness and FW were correlated with fruit hue angle and chroma, suggesting that red color may better develop in thick-skinned and/or small-sized pomegranates. Total anthocyanin content was negatively correlated with FW and fruit hue angle. AEAC was positively correlated with total anthocyanin and ascorbic acid contents, the latter one constituted a 15% contribution to AEAC. The associations found among physical and chemical traits suggest that consumers interested in a healthier produce should be directed to small and red pomegranates.

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