The Antidiabetic, Antioxidant properties in vitro of Moringa oleifera Flowers extracts grown in Sahara of Algeria

Moringa oleifera Flowers extracts grown in Algerian Sahara were evaluated antidiabetic and antioxidant activity by means using divers established in vitro systems, such as α-Amylase inhibitory assay, DPPH radical scavenging assay, Phosphomolybdenum assay (PM) and Ferric reducing power assay (FRAP). Moreover, the total content of phenols, flavonoids and tannins from (MFCE) and various fractions was measured using colorimetric methods. Results demonstrated that TPC varied between 95,50  0,42 and 10, 49  0,053 mg GAE/g WE, while TFC was between 17,00  0, 011 and 2,47  0,014 mg GAE/g WE, In this study TTC ranged between 2,96 0,016 and 1,30 0, 014 mg GAE/g WE. All capacities of DPPH radical scavenging, Phosphomolybdenum (PM) and Ferric reducing power (FRAP) were found best in (MFEF) (IC50= 0,159± 0,004mg/ml, AEAC = 42.37 ± 0.28 mM and AEAC =104.05±0.41 mM respectively). Add to this (MFEF) showed the highest α-amylase inhibitory activity (I= 38,92 %).

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Antioxidant and Anti-inflammatory Activities of Cynaroside from Elsholtiza bodinieri

Natural Product Communications ◽

10.1177/1934578x1801301122 ◽

2018 ◽

Vol 13 (11) ◽

pp. 1934578X1801301 ◽

Cited By ~ 4

Author(s):

Ying Zou ◽

Min Zhang ◽

Tingrui Zhang ◽

Junwen Wu ◽

Jun Wang ◽

...

Keyword(s):

Radical Scavenging ◽

Reducing Power ◽

Total Content ◽

Preparative Hplc ◽

Dpph Radical ◽

Anti Inflammatory ◽

Flavonoid Fraction ◽

Reducing Power Assay

The flavonoid fraction was obtained from Elsholtiza bodinieri Vaniot (EBV) by ethanol-reflux and liquid-liquid extraction. The total content of flavonoid was 179.55 mg/g, and the purity was 64.6%. Then cynaroside with the purity of 94% was isolated from the fraction by preparative HPLC and characterized by the combined usage of HPLC, ESI-MS, and NMR. The antioxidant activity of cynaroside was determined using 2 complementary methods, namely, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assay. The anti-inflammatory effect of cynaroside was investigated based on in-vitro and in-vivo experiment. The results showed that cynaroside from EBV scavenged DPPH radical and reduced Fe3+ to Fe2+ effectively, inhibited NO and ROS production in LPS-stimulated RAW264.7 cells and attenuated the inflammation in the mouse model significantly ( p < 0.01), which showed it to be a nutraceutical product in the food industry.

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Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3308 ◽

2020 ◽

Vol 11 (4) ◽

pp. 6262-6267

Author(s):

Krishnamoorthy Meenakumari ◽

Giridharan Bupesh ◽

Mayur Mausoom Phukan

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Biological Properties ◽

Scavenging Activity ◽

Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

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In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

BioMed Research International ◽

10.1155/2018/1824790 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Gauhar Rehman ◽

Muhammad Hamayun ◽

Amjad Iqbal ◽

Saif Ul Islam ◽

Saba Arshad ◽

...

Keyword(s):

Glucose Uptake ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Yeast Cells ◽

In Vitro Assays ◽

Dpph Radical ◽

Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

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Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

10.21203/rs.3.rs-930356/v1 ◽

2021 ◽

Author(s):

V. Renuka ◽

Chandragiri Nagaraja Rao Ravishankar ◽

Elavarasan Krishnamoorthy ◽

Abubacker Aliyamveetil Zynudheen

Keyword(s):

Thermal Behavior ◽

Antioxidant Activities ◽

Proteolytic Enzymes ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Structural Difference ◽

Dpph Radical ◽

Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

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Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502012000100004 ◽

2012 ◽

Vol 48 (1) ◽

pp. 31-37 ◽

Cited By ~ 9

Author(s):

Ariana Costa-Mugica ◽

Ana Elsa Batista- Gonzalez ◽

Diadelis Mondejar ◽

Yosdel Soto-López ◽

Victor Brito-Navarro ◽

...

Keyword(s):

Antioxidant Properties ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Ldl Oxidation ◽

Dpph Radical ◽

Polyphenol Content ◽

Reducing Activity ◽

Dpph Radical Scavenging ◽

And Oxidative Stress

LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural antioxidants have been studied as promising candidates. In the present study, the LDL oxidation inhibition activity of bioactive compounds from Halimeda incrassata seaweed. associated to antioxidant capacity, was evaluated in vitro. Experimental work was conducted with lyophilized aqueous extract and phenolic-rich fractions of the seaweed and their effect on LDL oxidation was evaluated using heparin-precipitated LDL (hep-LDL) with exposure to Cu2+ ions and AAPH as the free radical generator. H. incrassata had a protective effect for hep-LDL in both systems and the presence of phenolic compounds contributed to the activity where phenolic-rich fractions showed significant capacity for inhibition of oxidation mediated by Cu2+ ions. The observed effect could be related to the antioxidant potential of polar fractions evidenced by reducing activity and DPPH• radical scavenging. The results obtained in vitro further support the antioxidant and LDL oxidation inhibition properties of H. incrassata and further knowledge toward future phytotherapeutic application of the seaweed.

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Synthesis of 1-(5-Chloro-2-hydroxyphenyl)-5-oxopyrrolidine-3-carboxylic Acid Derivatives and Their Antioxidant Activity

Molecules ◽

10.3390/molecules24050971 ◽

2019 ◽

Vol 24 (5) ◽

pp. 971 ◽

Cited By ~ 3

Author(s):

Ingrida Tumosienė ◽

Kristina Kantminienė ◽

Ilona Jonuškienė ◽

Artūras Peleckis ◽

Sergey Belyakov ◽

...

Keyword(s):

Antioxidant Activity ◽

Carboxylic Acid ◽

Radical Scavenging ◽

Reducing Power ◽

Analysis Data ◽

Dpph Radical ◽

X Ray Diffraction ◽

X Ray ◽

Reducing Power Assay ◽

Dpph Radical Scavenging

A series of novel 1-(5-chloro-2-hydroxyphenyl)-5-oxopyrrolidine-3-carboxylic acid derivatives containing chloro, hydroxyl, isopropyl, nitro, nitroso, and amino substituents at benzene ring and 1-(5-chloro-2-hydroxyphenyl)-5-oxopyrrolidine-3-carbohydrazide derivatives bearing heterocyclic moieties were synthesized. Antioxidant activity of the synthesized compounds was screened by DPPH radical scavenging method and reducing power assay. A number of compounds were identified as potent antioxidants. Antioxidant activity of 1-(5-chloro-2-hydroxyphenyl)-4-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)pyrrolidin-2-one has been tested to be 1.5 times higher than that of a well-known antioxidant ascorbic acid. 1-(5-Chloro-2-hydroxyphenyl)-4-(4-methyl-5-thioxo-4,5-dihydro-1H-1,2,4-triazol-3-yl)pyrrolidin-2-one has shown 1.35 times higher antioxidant activity than that of vitamin C by DPPH radical scavenging method and optical density value of 1.149 in reducing power assay. The structure of 1-(5-chloro-2-hydroxyphenyl)-N-(1,3-dioxoisoindolin-2-yl)-5-oxopyrrolidine-3-carboxamide was unambiguously assigned by means of X-ray diffraction analysis data.

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COMPARATIVE EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT POTENCY OF DIFFERENT EXTRACTS OBTAINED FROM MEMECYLON SPECIES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15695 ◽

2017 ◽

Vol 9 (2) ◽

pp. 187

Author(s):

Tumkur Ramasetty Bharathi ◽

Harishchandra Sripathy Prakash

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Methanol Extracts ◽

Soxhlet Apparatus ◽

Antioxidant Potency ◽

Reducing Power Assay ◽

Different Doses

Objective: Memecylon species is being extensively used in traditional medicine for the treatment of skin disorders and it is proved to possess antidiabetic and anti-inflammatory properties. The present investigation was to study the effect of different solvent extracts of five Memecylon species such as M. umbellatum, M. talbotianum, M. edule, M. malabaricum and M. wightii on antidiabetic and antioxidant effects.Methods: Plant extracts were prepared using soxhlet apparatus using different solvents such as hexane, ethyl acetate, methanol and water and obtained extracts were subjected to antidiabetic (α-amylase and α-glucosidase inhibition assays) and antioxidant (2, 2-Diphenyl-2-Picryl Hydrazyl hydrate (DPPH), 2,2-Azino-bis (3-ethyl benzothiazoline-6-Sulfonic acid)diammonium salt (ABTS), Superoxide radical scavenging assay (SRSA) and reducing power assays) evaluated at different doses.Results: Methanol extracts of all five Memecylon species exhibited effective antidiabetic and antioxidant properties among them methanol extracts of M. malabaricum and M. talbotianum have highest biological activity. For α-amylase IC50 value for both M. malabaricum and M. talbotianum was found to be 100 and 130 µg/reaction and IC50 value for α-glucosidase was found to be 6.1 and 7.8 µg/reaction respectively. For DPPH the IC50value was found to be 190 µg/reaction, for ABTS 31-39 µg/reaction, for SRSA 950-1200 µg/reaction and for reducing power assay 420-490 µg/reaction respectively.Conclusion: The results indicate that methanol extracts of M. malabaricum and M. talbotianum possess potent in vitro antidiabetic and antioxidant activities compared to other Memecylon species.

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Experimental Assessment ofMoringa oleiferaLeaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential UsingIn VitroandIn VivoAssays

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/519084 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 63

Author(s):

Suaib Luqman ◽

Suchita Srivastava ◽

Ritesh Kumar ◽

Anil Kumar Maurya ◽

Debabrata Chanda

Keyword(s):

Aqueous Extract ◽

Moringa Oleifera ◽

Evaluation Studies ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Ethanolic Extract ◽

Dependent Manner ◽

Concentration Dependent Manner

We have investigated effect ofMoringa oleiferaleaf and fruit extracts on markers of oxidative stress, its toxicity evaluation, and correlation with antioxidant properties usingin vitroandin vitroassays. The aqueous extract of leaf was able to increase the GSH and reduce MDA level in a concentration-dependent manner. The ethanolic extract of fruit showed highest phenolic content, strong reducing power and free radical scavenging capacity. The antioxidant capacity of ethanolic extract of both fruit and leaf was higher in thein vitroassay compared to aqueous extract which showed higher potentialin vivo. Safety evaluation studies showed no toxicity of the extracts up to a dose of 100 mg/kg body weight. Our results support the potent antioxidant activity of aqueous and ethanolic extract ofMoringa oleiferawhich adds one more positive attribute to its known pharmacological importance.

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Chemical Composition and Antioxidant Properties of Essential Oils from Peppermint, Native Spearmint and Scotch Spearmint

Molecules ◽

10.3390/molecules24152825 ◽

2019 ◽

Vol 24 (15) ◽

pp. 2825 ◽

Cited By ~ 9

Author(s):

Zhaohai Wu ◽

Bie Tan ◽

Yanhong Liu ◽

James Dunn ◽

Patricia Martorell Guerola ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oils ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

C Elegans ◽

Reducing Power Assay

Natural antioxidants have drawn growing interest for use in animal feed and the food industry. In the current study, essential oils (EOs) obtained from hydrodistillation of three mentha species, including Mentha piperita (peppermint), Mentha spicata (native spearmint) and Mentha gracilis (Scotch spearmint), harvested in the Midwest region in the United States, were analyzed for their chemical composition using gas chromatography-mass spectrometry, and their antioxidant properties were assessed through chemical assays, in vitro cell culture modeling and in Caenorhabditis elegans (C. elegans). The activity of ferric iron reduction and free-radical scavenging capacity were assessed through chemical-based assays, including the reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and Trolox equivalent antioxidant capacity assay (TEAC). Subsequently, the capacity of EOs to mitigate lipid peroxidation was analyzed at various doses using fresh liver homogenates from pigs. A porcine jejunum epithelial cell line (IPEC-J2) was employed as in vitro model to study the cellular antioxidant activity of the mint EOs. Finally, the effectiveness of mint EOs to alleviate acute systemic oxidative damage were evaluated in vivo using C. elegans. Data were analyzed by the MIXED procedure of SAS. Contrast statement was performed to assess linear or quadratic effects of mint EOs given at various doses. All three EOs are mostly composed of monoterpenes and their derivatives (76–90%), but differed in the major compounds, which are menthol and menthone (50%) in peppermint EO and carvone (70%) in spearmint EOs. Three mint EOs demonstrated prominent radical scavenging and Fe3+ reducing activity in chemical-based assays. In comparison with native and Scotch spearmint EOs, peppermint EO had the lowest (p < 0.05) half maximal effective concentration (EC50) in DPPH and TEAC assays and higher efficacy in the reducing power assay. All three EOs exhibited equivalent activity in mitigation of chemical-induced lipid peroxidation in liver tissues in a dose-dependent manner (linear, p < 0.001). The maximal cellular antioxidant activity (CAA) was observed at 5 µg/mL for peppermint, and 100 µg/mL for native and Scotch spearmint EOs. The addition of 25 µg/mL of both spearmint EOs increased (p < 0.05) cellular concentrations of glutathione in H2O2-treated IPEC-J2 cells, suggesting enhanced endogenous antioxidant defense. Supplementation of 100 µg/mL of peppermint or Scotch spearmint EO significantly increased (p < 0.05) the survival rate of C. elegans in response to H2O2-induced oxidative stress. The protective effect is comparable to that of supplementation of 10 µg/mL of ascorbic acid. However native spearmint EO failed to reduce the death rate within the same supplementation dose (10–200 μg/mL).

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ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF CYCLOSORUS INTERRUPTUS (WILLD.) H. ITÔ AND PRONEPHRIUM NUDATUM (ROXB. EX GRIFF.) HOLTTUM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i9.32039 ◽

2019 ◽

pp. 87-94

Author(s):

ABHIJIT MITRA ◽

MANABENDRA DUTTA CHOUDHURY ◽

PRAKASH ROY CHOUDHURY ◽

DEEPA NATH ◽

SUBRATA DAS ◽

...

Keyword(s):

Methanol Extract ◽

Radical Scavenging ◽

Reducing Power ◽

Natural Antioxidants ◽

Total Phenolic ◽

Phytochemical Analysis ◽

In Vitro Systems ◽

Reducing Power Assay ◽

Phenolic And Flavonoid

Objectives: The work aims to screen the antioxidative potentials of different crude extracts of the fronds of two medicinally important pteridophytes of Southern Assam, India, namely, Cyclosorus interruptus (Willd.) H. Itô and Pronephrium nudatum (Roxb. ex Griff.) Holttum. Methods: Frond extracts of the pteridophytes were prepared by Soxhlet hot extraction method. Total phenolic content (TPC) and total flavonoid content (TFC) of the hexane, ethyl acetate, acetone, and methanol extracts of the fronds of the plants were done by following standard protocol. In vitro assessment of the antioxidative behavior of the extracts was performed using standard 2,2-diphenyl-1-picrylhydrazyl scavenging assay, reducing power assay, metal chelating assay, hydroxyl, superoxide, and 2,2’- azino-bis (3-ethylbenzothiazoline - 6 – sulfonic acid) radical scavenging methods in different in vitro systems. Results: Preliminary phytochemical analysis implicated the presence of phenolic and flavonoid compounds in all the frond extracts. The methanol extract of the fronds of both the plants showed maximum phenolic and flavonoid contents in comparison to the other extracts, however, that of C. interruptus was found to be higher than P. nudatum. Antioxidative potentials of the said extracts were also found to be impressive and noteworthy. The decreasing order of the antioxidative efficacies of the extracts was found to be same as that of TPC and TFC of the extracts. Conclusion: It is pertinent to comment that the methanol extract of the fronds of both the plants may be treated as a potential source of natural antioxidants.

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