scholarly journals Research on degradation of silk fibroin by combination of electron beam irradiation and hydrothermal processing

2014 ◽  
Vol 4 (2) ◽  
pp. 42-49
Author(s):  
Thi Kim Lan Nguyen ◽  
Van Phu Dang ◽  
Anh Quoc Le ◽  
Quoc Hien Nguyen
Keyword(s):  
Electron Beam ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Sodium Hydroxide Solution ◽  
Silk Protein ◽  
Hydrothermal Processing ◽  
X Ray Diffraction ◽  
Silk Fibers ◽  
Sds Page ◽  
Ft Ir

Silk fibers and silk proteins have been demonstrated to be useful to apply in the textile industry, biomedical, cosmetics, pharmaceuticals. In this study, the effects of electron beam (EB) irradiation combined with hydrothermal processing to the solubility of silk fibroin and generation of soluble silk protein were investigated. The solubility of unirradiated and irradiated fibroin samples were greater than 80 % when hydrothermal degradation was performed in the sodium hydroxide solution at an appropriate concentration of 0.05 M. However, the solubility of irradiated fibroin was greater than that of unirradiated sample. The soluble silk protein content increased from 0.462 to 0.653 mg protein/mg silk fibbroin when irradiation doses increased from 0 to 200 kGy, respectively. The molecular weight of protein was determined by SDS-PAGE method. The characteristics of silk protein were confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and X-ray diffraction (XRD).

Copper (II) Removal by Using Manganese Ferrite in PVA-Alginate Beads

2015 ◽  
Vol 735 ◽  
pp. 177-181
Author(s):  
Ee Ting Wong ◽  
Pei Cheng Teh ◽  
Kian Hwa Chan ◽  
Ani Idris
Keyword(s):  
Sodium Hydroxide Solution ◽  
Alginate Beads ◽  
Precipitation Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
Drinking Water Standard ◽  
Ft Ir ◽  
Co Precipitation ◽  
Control Study ◽  
Alginate Matrix

The magnetic nanoparticles of manganese-doped magnetite (Mn-Fe2O4) were synthesized by the simple co-precipitation method. The stable Mn2+ and Fe3+ salts in the ratio of 1:2 in aqueous solution, were added into the sodium hydroxide solution to form the Mn-Fe2O4 precipitate at temperature of 95°C. The synthesized nanoparticles were then characterized by Fourier Transform-Infrared Spectroscopy (FT-IR) and X-ray diffraction spectroscopy (XRD). It was then entrapped in the PVA-alginate matrix to form the nanophotoadsorbent in beads form. The synthesized nanoparticles embedded bead was characterized by Scanning Electron Microscopy (SEM). The effects of various parameters, such as contact time, pH, nanoparticles dosage were investigated. The control study was also performed to reveal the performance of photo-adsorbent towards the Cu (II) removal under the light and dark conditions. It was found that the removal efficiency of the Cu (II) achieved 97.07% (1.46ppm) which complied to the WHO drinking water standard of less than 1.5ppm after 180 min treatment.

scholarly journals Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Huijing Zhao ◽  
Siyong Xiong ◽  
Mingzhong Li ◽  
Qiang Zhang ◽  
Guiyang Liu
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Gelation Time ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
X Ray ◽  
Silk Fibroins ◽  
Ft Ir ◽  
Additional Formation ◽  
Ir And Raman

Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of theAntheraea pernyi(A. pernyi) SF solution was significantly shorter than that of theBombyx mori(B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins ofA. pernyiandB. mori. The gelation time of bothA. pernyiSF andB. moriSF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation ofβ-sheets.

Preparation of Transparent Water-Insoluble Silk Fibroin Films

2011 ◽  
Vol 175-176 ◽  
pp. 79-84
Author(s):  
Min Qing Luo ◽  
Cen Cen Zhang ◽  
Shen Zhou Lu
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Light Transmittance ◽  
Human Cornea ◽  
X Ray Diffraction ◽  
X Ray ◽  
Blend Films ◽  
Structure Surface ◽  
Ft Ir ◽  
Transparent Water

The transparent water-insoluble silk fibroin(SF) films were casted from the mixture solution of silk fibroin and xylitol/mannitol. The structure, surface morphology, solubility, mechanical properties and light transmittance of the blend films were measured. FT-IR, X-ray diffraction retuslts indicated that the films were mainly composed of Silk I structure. SEM showed the blend films with xylitol were miscible, whereas the blend films with mannitol had phase-separated structure. There were lots of nanopores in the blend films in the wet state. The insoluble SF /xylitol films had excellent mechanical properties while the SF / mannitol films were brittle. The mechanical property of SF/alcohol blend films were consistent with the human cornea in wet state.When the contents of xylitol were 10% and 20%, the blend films had high light transmittance which were similar to human cornea. In summary, the SF /xylitol film containing 10% xylitol provides a great potential to act as repairing materials for cornea.

High Functional Silk Fibroin Polyester Fabric

2011 ◽  
Vol 332-334 ◽  
pp. 799-803
Author(s):  
Gang Xian Zhang ◽  
Nan Han ◽  
Wei Hu ◽  
Feng Xiu Zhang
Keyword(s):  
Silk Fibroin ◽  
Glycidyl Ether ◽  
Polyester Fabric ◽  
Air Permeability ◽  
Sucrose Ester ◽  
X Ray Diffraction ◽  
Softening Function ◽  
X Ray ◽  
Polyester Fibers ◽  
Ft Ir

Though polyester fabric has favorable characteristics, it is not comfortable to wear because of its hydrophobic characteristics Silk fibroin has lots of polar and electric groups, and its biocompatible is very good. In this paper polyester fabric was encased sucrose ester to endue polyester fibers lots of –OH, then sucrose eater glycidyl ether (SEGE) which not only had crosslink function but also had softening function was used to graft silk fibroin on polyester fabric. FT-IR spectra showed silk fibroin was grafted on polyester fabric; DSC showed thermostability of fabric kept very well; X-ray diffraction spectra showed the structure of some silk fibroin grafted on polyester fabric was -sheet. With the increase of grafting rate of silk fibroin on polyester fabric, the broken strength of polyester fabric grafted silk fibroin did not decrease; cockle elastics kept very well; antistatic property and moisture regain increased greatly, whiteness and air permeability kept very well and fabric was still soft enough. The polyester fabric grafted silk fibroin was high functional.

PEG Decorated Glycine Capped Mn-Ferrite Nanoparticles Synthesized by Co-Precipitation Method for Biomedical Application

2013 ◽  
Vol 829 ◽  
pp. 274-278 ◽  
Author(s):  
Mahdokht Faham ◽  
Hooman Shokrollahi ◽  
Gholamhossein Yousefi ◽  
Sahar Abbasi
Keyword(s):  
Particle Size ◽  
Biomedical Application ◽  
Sodium Hydroxide Solution ◽  
Physicochemical Characteristics ◽  
Precipitation Method ◽  
X Ray Diffraction ◽  
Ft Ir ◽  
Surface Particle ◽  
Poly Ethylene ◽  
Co Precipitation

Our goal is to develop methoxy poly (ethylene glycol) (m-PEG) decorated, glycine capped magnetic nanoparticles (MNPs) with proper physicochemical characteristics including particle size and magnetic property. MNP were synthesized by a biocompatible chemical co-precipitation of Mn2+and Fe3+in a sodium hydroxide solution. In order to covalently modify nanoparticles surface by previously prepared m-PEG aldehyde, glycine was used as linker. X-ray diffraction analysis showed successful formation of pure nanocrystalline single phase of Mn-Ferrite and FT-IR spectroscopy approved that m-PEG and glycine were covalently bound to nanoparticles surface. Particle size study showed significant change after modification. In addition, the Vibrating sample magnetometry was done for measuring the magnetic properties (Ms: 52 emu/g) and showing the superparamagnetism behavior.

scholarly journals Structural, optical, morphological and SHG studies on 8 MeV electron beam irradiated Sr(HCOO)2·2H2O crystal

2020 ◽  
Vol 38 (2) ◽  
pp. 236-247
Author(s):  
S. Muthupoongodi ◽  
S. Theodore David Manickam ◽  
C.K. Mahadevan ◽  
J. Angel Mary Greena ◽  
S. Balakumar ◽  
...  
Keyword(s):  
Electron Beam ◽  
Second Harmonic ◽  
Solvent Evaporation Method ◽  
X Ray Diffraction ◽  
Crystalline Perfection ◽  
X Ray ◽  
Ft Ir ◽  
Shg Efficiency ◽  
Thermal Stability Of ◽  
Different Doses

AbstractStrontium formate dihydrate single crystals have been grown by the slow solvent evaporation method and subjected to 8 MeV electron beam (EB) irradiation at different doses, viz. 1 kGy, 10 kGy, 50 kGy and 100 kGy. The irradiated crystals have been studied by various techniques such as FT-IR, Raman, and UV-Vis-NIR spectral analyses, powder, and high resolution X-ray diffraction, thermogravimetric analysis (TG/DTA) and second harmonic generation (SHG) measurements. The morphological features of the non-irradiated and irradiated crystals were analyzed by SEM and optical microscopy studies. It has been noticed that the SHG efficiency increases while the crystalline perfection and thermal stability of the irradiated crystals decrease with the increase of EB irradiation dose. The results indicate a significant overall tuning of physical properties of strontium formate dihydrate crystals due to irradiation.

scholarly journals Preparation and Characterization of TiO2 Nanostructure by TiCl4 Hydrolysis with Additive NaOH

2018 ◽  
Vol 28 (2) ◽  
pp. 100 ◽  
Author(s):  
Rashed Taleb Rasheed
Keyword(s):  
Sodium Hydroxide Solution ◽  
Water Solution ◽  
Ultra Violet ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Atomic Force ◽  
Alcohol Water ◽  
Ft Ir ◽  
Hydrolysis Of

Titanium dioxide (TiO2) nanostructures were synthesized via the hydrolysis of TiCl4 in alcohol / water solution/with sodium hydroxide solution in the ice-bath (0-5 ◦C). The particles were char-acterized by using X-ray diffraction technique (XRD), spectroscopy of Ultra Violet-Visible (UV / Visible) and infrared (FT-IR), atomic force microscope (AFM) and scanning electron micro-scope (SEM) analysis were used in order to gain information about the material, morphology, size and the shape of the particles

scholarly journals High Molecular Weight Silk Fibroin Prepared by Papain Degumming

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2105
Author(s):  
Yanfei Feng ◽  
Jiaming Lin ◽  
Longxing Niu ◽  
Ying Wang ◽  
Zhiling Cheng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Molecular Weight ◽  
Amino Acid ◽  
High Molecular Weight ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Picric Acid ◽  
Complete Removal ◽  
Silk Fibers ◽  
Yellow Color

A major challenge for the silk textile industry and for the process of silk-based biomaterials is to find a degumming method that can completely remove sericin while avoiding obvious hydrolysis damage to the silk fibroin. In this study, papain was used to degum Bombyx mori silk fibers under nearly neutral conditions based on the specificity of papain to sericin. The degumming efficiency was investigated, as well as the mechanical properties and molecular weight of the sericin-free silk fibroin. The results indicated that increasing the papain concentration aided in sericin removal, as the concentration increased to 3.0 g/L, the degummed fibers showed a clean, smooth surface morphology and exhibited a yellow color when stained by picric acid and carmine, confirming the complete removal of sericin from silk fibroin. Furthermore, an analysis of the amino acid composition indicated that the silk fibroin suffered less damage because papain specifically cleaved the binding sites between L-arginine or L-lysine residue and another amino acid residue in sericin, leading to a significantly higher molecular weight and improved tensile strength compared to traditional sodium carbonate degumming. This study provides a novel degumming method which cannot only completely remove sericin, but also maintain the original strong mechanical properties and high molecular weight of silk fibroin.

scholarly journals THE FINE STRUCTURE OF SILK FIBROIN

1967 ◽  
Vol 32 (2) ◽  
pp. 289-295 ◽  
Author(s):  
M. G. Dobb ◽  
R. D. B. Fraser ◽  
T. P. Macrae
Keyword(s):  
Fine Structure ◽  
Silk Fibroin ◽  
Crystalline Material ◽  
Fiber Axis ◽  
X Ray Diffraction ◽  
Silk Fibers ◽  
X Ray ◽  
Considerable Length ◽  
Bombyx Mori Silk ◽  
Lateral Extent

The fine structure of Bombyx mori silk fibroin was investigated by electron microscopy and X-ray diffraction techniques. Examination of silk fibers fragmented with ultrasonic radiation and negatively stained revealed the presence of ribbon-like filaments of well-defined lateral dimensions. Analysis of the breadths of the equatorial reflections in the X-ray diffraction pattern of fibroin yielded similar dimensions for the lateral extent of the crystallites. It is concluded that the crystalline material in B. mori silk fibroin is in the form of ribbon-like filaments of considerable length parallel to the fiber axis and of lateral dimensions approximately 20 x 60 A.

Structure and Mechanical Property of Silk Fiber under Gamma Radiation

2011 ◽  
Vol 175-176 ◽  
pp. 85-89 ◽  
Author(s):  
Wei Wei Yao ◽  
Zhi Wu Liu ◽  
Hong Gen Yi ◽  
Jian Nan Wang
Keyword(s):  
Gamma Radiation ◽  
Radiation Intensity ◽  
Breaking Strength ◽  
Biological Application ◽  
X Ray Diffraction ◽  
Molecular Conformations ◽  
Silk Fibers ◽  
X Ray ◽  
Sds Page ◽  
Intermolecular Bonding

An attempt to change the structure of silk fibers and their properties for the biological application was studied by utilizing gamma radiation in various Co60 intensities (0 kGy, 30 kGy, 50 kGy, 100 kGy, 200 kGy, 500 kGy, 1000 kGy, 2000 kGy, 3000 kGy). With the increase of the gamma radiation intensity, SEM result shows that cracks and fragments were formed between microfibrils of the irradiated fiber significantly. Simultaneously SDS-PAGE results give the evidence that the molecular weight of the fibroin diminished. Furthermore, the breaking strength and elongation of irradiated fibers decreased gradually with the increasing Co60 intensity. Although no significant changes of the molecular conformations were found by FTIR and X-ray diffraction, the effects on molecular interactions of the silk fibroin, such as peptide bonding, hydrogen bond and intermolecular bonding force, were obviously observed and enhanced gradually with the increase of gamma radiation intensity.

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