IN VITRO ANTHELMINTIC ACTIVITY OF ARTEMISIA MARITIMA LINN.

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (08) ◽  
pp. 39-41
Author(s):  
M. M Hayat ◽  
◽  
S Sharma ◽  
D Vashishth ◽  
R. Kaur
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Aqueous Extracts ◽  
Reference Standard ◽  
Dose Levels ◽  
Artemisia Maritima

Ethanolic and aqueous extracts of the flower heads of Artemisia maritima Linn. were evaluated for anthelmintic activity on Indian earthworms Pheritima posthuma, using albendazole as reference standard. The extracts caused paralysis followed by death of the worms at all tested dose levels. Ethanolic extract was found to be more potent than the reference control albendazole. The result supports the traditional claims of Artimisia maritime as a powerful anthelmintic.

scholarly journals IN VITRO ASSESSMENT OF ANTHELMINTIC AND ALPHA-AMYLASE INHIBITION OF SCHLEICHERA OLEOSA (LOUR.) OKEN LEAF EXTRACTS

2018 ◽  
Vol 11 (9) ◽  
pp. 487
Author(s):  
Shambaditya Goswami ◽  
Ravindra Pal Singh
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Alpha Amylase ◽  
Control Treatment ◽  
Aqueous Extracts ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Schleichera Oleosa

Objective: The present study deals with the effects of Schleichera oleosa (Lour.) Oken leaf extracts on helminths and alpha-amylase inhibition. Identification of phytochemicals and physicochemical analysis were also performed.Methods: Different concentrations (25, 50, and 100 mg/ml) of petroleum ether, acetone, chloroform, ethanol, and aqueous extracts of the leaf were used to examine the effects. For the evaluation of in vitro anthelmintic activity, several earthworms (Eisenia fetida, Perionyx excavates, and Pheretima posthuma) and nematode (Ascaridia galli) were taken, while albendazole was used as a standard drug and Tween 80 (3%) in normal saline (0.9% NaCl) was considered as a control treatment. In vitro alpha-amylase inhibition of different extracts (10–100 mg/ml) was done spectrophotometrically by dinitrosalicylic acid - starch azure method.Results: The ethanolic extract showed the maximum presence of phytochemicals among all the extracts, which included alkaloids, tannins, flavonoids, saponin glycosides, phenolic compounds, resins, and amino acids. The outcomes of the determination of physicochemical parameters and fluorescence characters provided the satisfactory results. Significant anthelmintic activity was established by the ethanolic and aqueous extracts of the leaf among all the extracts and the responses, so observed, were dose responsive. Inhibition of alpha-amylase by ethanolic and aqueous extracts was significant with the IC50 value of 36.63 and 73.94 μg/ml, respectively, when compared to standard acarbose.Conclusion: The ethanolic extract was the more potent candidate for both the effects, and the effect of extract was best against A. galli, P. posthuma, and E. fetida at higher concentration. Isolation and characterization of therapeutic constituents would be the future interest.

In Vitro Antimicrobial and Antioxidant Activities of Organic and Aqueous Extracts of Bacillus clausii KP10

2019 ◽  
Vol 41 (1) ◽  
pp. 161-161
Author(s):  
Farzana Nighat and Zahid Mushtaq Farzana Nighat and Zahid Mushtaq
Keyword(s):  
Hot Springs ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Current Case ◽  
Bacillus Clausii

Humans and plants are experiencing different kinds of diseases. Many of these diseases are mainly caused by microorganisms like fungi, bacteria and viruses that adversely affect their hosts. Bioactive compounds produced by bacteria can be used to treat the infections caused by these microorganisms. Free radicals that can cause serious damage to the cell can be repressed by antioxidants that are produced by bacteria as secondary compounds. The current case study was therefore planned to investigate the antimicrobial and antioxidant potentials of the organic and aqueous extracts of Bacillus clausii KP10 isolated recently by our group from hot springs of Tatta Pani, Poonch, Azad Kashmir. The antimicrobial activities were assessed using well diffusion method against two Gram positive bacteria; B. subtilis, and S. aureus, and two Gram negative bacteria; E. coli and P. multocida and three fungal strains; A. niger, A. parasiticus and A. ustus. Antioxidant activities of the extracts were done by DPPH free radical scavenging assay. In our experiments the maximum zones of inhibition were shown by ethanolic extract against P. multocida (21 mm), S. aureus (13 mm) and methanol soluble fraction showed zone of inhibition against A. ustus (21 mm) as compared to the standard ciprofloxacin for bacteria and as antifungal agents with terbinafine. In our studies n-hexane fraction of B. clausii KP10 showed maximum 88.67% antioxidant activity. Finally, results were analyzed statistically through analysis of variance (ANOVA). Hence it is proved that the bioactive fractions obtained from B. clausii KP10 are good source of antimicrobials and antioxidants and they can be used as medicines in future for the respective treatments.

scholarly journals IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT

2016 ◽  
Vol 9 (1) ◽  
pp. 189
Author(s):  
Pinky Sarmah ◽  
Nako Kobing ◽  
Jyotchna Gogoi ◽  
Ananta Madhab Dutta
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Biological Properties ◽  
Death Time ◽  
Control Sets ◽  
Point Analysis ◽  
Soxhlet Apparatus ◽  
And Control

<p><strong>Objective</strong>:<strong> </strong>The present study aims to investigate <em>in vitro</em> anthelmintic and anti-amylase properties of the ethanolic fruit extract of <em>Garcinia pedunculata</em>.</p><p><strong>Methods</strong>:<strong> </strong>For the study, mature fruits of <em>G. pedunculata</em> were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of <em>G. pedunculata</em> was used for evaluation of <em>in vitro</em> anthelmintic and anti-amylase activities. <em>In vitro</em> anthelmintic activity was evaluated in animal models, <em>Pheretima posthuma</em>, an earthworm species. <em>In vitro</em> anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].</p><p><strong>Results</strong>:<strong> </strong>Ethanolic extract of <em>G. pedunculata</em> showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of <em>G. pedunculata</em> showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.</p><strong>Conclusion</strong>:<strong> </strong>It could be concluded that ethanolic extract of <em>G. pedunculata</em>has biological properties which could be utilised in medicine by characterising its bioactive components.<p> </p>

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

2020 ◽  
Vol 2 (29) ◽  
pp. 32-36
Author(s):  
Semwal Amit Negi Sweta
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Disk Diffusion Method ◽  
Zanthoxylum Armatum ◽  
Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

scholarly journals Anthelmintic and Cytotoxic Activities of Two Medicinal Plants: Polygonum viscosum and Aphanamixis polystachya Growing in Bangladesh

2014 ◽  
Vol 6 (2) ◽  
pp. 339-345 ◽  
Author(s):  
M. N. Amin ◽  
M. S. Majumder ◽  
M. M. R. Moghal ◽  
S. Banik ◽  
A. Kar ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Reference Standard ◽  
Cytotoxic Potential ◽  
Lc50 Value ◽  
Vincristine Sulphate ◽  
Dose Dependent

The present study was designed to investigate in vitro anthelmintic and cytotoxic activities of crude methanolic extract of two plants(Polygonum viscosum and Aphanamixis polystachya) grown in Bangladesh. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. The crude methanolic extract of Polygonum viscosum showed significant cytotoxic potential (LC50 value of 6.34 ?g/ml) among all the fractions comparing with that of standard vincristine sulphate (0.825 ?g/ml). Besides, the LC50 values of crude methanolic extract, pet ether and chloroform extracts of Aphanamixis polystachya showed good cytotoxic activities 11, 10.36, and 16.45 µg/ml, respectively. The other study was undertaken to evaluate anthelmintic activity (using Pheretima posthuma model) where piperazine was used as reference standard. The crude methanolic extract of Polygonum viscosum leaves produced a significant anthelmintic activity in dose dependent manner and the activity of crude extract was comparable with that of standard drugs. Besides, the Aphanamixis polystachya extract revealed moderate anthelmintic activity. Here, the anova testing was done with the P < 0.05. Further studies are suggested to determine the active compounds responsible for the anthelmintic and cytotoxic activities of these two plant extracts.   Keywords: Anthelmintic; Cytotoxic; Medicinal plant; Aphanamixis polystachya; Polygonum viscosu.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   doi: http://dx.doi.org/10.3329/jsr.v6i2.17299 J. Sci. Res. 6 (2), 339-345 (2014) 

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAF RACHIS OF AZADIRACHTA INDICA Linn. (NEEM)

2017 ◽  
Vol 03 (01) ◽  
pp. 8-12
Author(s):  
Gavalapu Venkateswara Rao ◽  
Abhinav kanagarla ◽  
Allamraju Satyakeertana ◽  
Vinukonda Alekhya ◽  
Dammu Saipriya ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Phytochemical Screening

scholarly journals Effectiveness of flor-de-seda and pomegranate aqueous extracts on eggs of the Heterakoidea Superfamily isolated from naturally infected japanese quails

2020 ◽  
Vol 21 ◽  
Author(s):  
Patrícia Araujo BRANDÃO ◽  
David Rwbystanne Pereira da SILVA ◽  
Giuliana Garcia DINIZ ◽  
Vinícius Longo Ribeiro VILELA
Keyword(s):  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Punica Granatum ◽  
Positive Control ◽  
Control Treatment ◽  
Calotropis Procera ◽  
Aqueous Extracts ◽  
Similar Action ◽  
Japanese Quails

ABSTRACT The present study aimed to evaluate the in vitro anthelmintic efficacy of flor-de-seda (Calotropis procera S.W.) and pomegranate (Punica granatum L.) extracts on gastrointestinal nematode eggs of Japanese quails. Stool samples were collected from a herd of 5,000 adult birds raised on the floor at a commercial farm in the state of Ceará, Brazil. The samples were packed in plastic bags, kept refrigerated and transported for laboratory analysis. The eggs were placed in 2.5 ml of the suspension and 2.5 ml of flor-de-seda and pomegranate extracts in concentrations of 50; 25; 12.5; 6.25 and 3.12%. Readings were subsequently taken at 24, 48 and 72 hours. The aqueous pomegranate extract at 25 and 50% was more efficient on the larvae hatching. The flor-de-seda aqueous extract in both concentrations and evaluation periods showed similar action to that of the positive control treatment (Albendazole 10%). The pomegranate and flor-de-seda aqueous extracts have anthelmintic activity on the development of larvae in eggs of the Heterakoidea Superfamily and are therefore presented as an alternative to replace the drugs commonly used to control helminths in Japanese quails.

scholarly journals EVALUATION OF IN VITRO ANTIUROLITHIATIC ACTIVITY OF CHLORIS BARBATA

2018 ◽  
Vol 10 (3) ◽  
pp. 65
Author(s):  
Niharika M. ◽  
Harshitha V. ◽  
Ashwini P. ◽  
Srivinya B. ◽  
Himabindhu J. ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Aqueous Extracts ◽  
Calcium Oxalate Crystals ◽  
Leaf Extracts ◽  
Oxalate Crystals ◽  
Crude Plant Extract ◽  
Chloris Barbata

Objective: The present study was undertaken to evaluate the in vitro antiurolithiatic activity of the medicinal plant Chloris barbata.Methods: The crude plant extract was prepared by Soxhlet extraction method.Results: Both Ethanolic and Aqueous extracts showed their maximum efficiencies in the dissolution of calcium oxalate crystals. Ethanolic extract was even more efficient than Aqueous extract in the dissolution of calcium oxalate crystals. Our results have clearly indicated that the Aqueous and Ethanolic leaf extracts of Chlorisbarbata were quite promising for further studies in this regard. In this study, Neeri was used as standard drug.Conclusion: This study has given primary evidence for Chloris barbata as the plant which possess antiurolithiatic property.

Anthelmintic efficacy ofAchillea millifoliumagainst gastrointestinal nematodes of sheep:in vitroandin vivostudies

2008 ◽  
Vol 82 (3) ◽  
pp. 227-233 ◽  
Author(s):  
K.A. Tariq ◽  
M.Z. Chishti ◽  
F. Ahmad ◽  
A.S. Shawl
Keyword(s):  
Body Weight ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Water Soluble ◽  
Aqueous Extracts ◽  
Anthelmintic Efficacy ◽  
Ethanolic Extracts ◽  
Motility Inhibition

AbstractAchillea millifoliumLinn., commonly called ‘Pehl-ghasa’, is used by farmers in traditional animal health care as a vermifuge. The objective of this study was to evaluate the anthelmintic efficacy of crude aqueous extracts and crude ethanolic extracts of entireA. millifoliumagainst the gastrointestinal nematodes of sheep. The worm motility inhibition assay was used forin vitrostudies and faecal egg count reduction assay was used forin vivostudies.In vitrostudies revealed significant anthelmintic effects of aqueous extracts and ethanolic extracts on liveHaemonchus contortusworms (P < 0.05) as evident from their paralysis and/or death at 8 h post exposure. Aqueous extracts ofA. millifoliumresulted in a mean worm motility inhibition of 94.44%, while ethanolic extracts resulted in mean worm motility inhibition of 88.88%. The mean mortality index of aqueous extracts was 0.95 while for ethanolic extracts it was 0.9. The lethal concentration 50 was 0.05 mg ml− 1for aqueous extracts and 0.11 mg ml− 1for ethanolic extracts. Thein vivoanthelmintic activity of aqueous and ethanolic extracts ofA. millifoliumdemonstrated a maximum (88.40%) nematode egg count reduction in sheep treated with aqueous extracts at 2 g kg− 1body weight on day 15 after treatment. Ethanolic extracts resulted in a maximum of 76.53% reduction in faecal egg counts on day 15 after treatment with 2 g kg− 1body weight. Thus, the aqueous extracts exhibited greater anthelmintic activity under bothin vitroandin vivoconditions, and could be due to the presence of water-soluble active principle/s inA. millifolium. It is concluded that the entire plant ofA. millifoliumpossesses significant anthelmintic activity and could be a potential alternative for treating cases of helminth infections in ruminants.

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