Effect of Larvicidal Extract N-Hexane Lime Leaves (Citrus hystrix) on Larva Instar III Mosquito (Culex quinquefasciatus)

Culex quinquefasciatus mosquitoes are a vector of transmission of several types of diseases, such as filariasis, Japanese encephalitis, and dirofilariasis. Larval control is the key strategy of disease control programs caused by vectors around the world because it can prevent larvae to enter the adult stage. Control of mosquito larvae that are often used is chemically controlled. Citrus hystrix is a natural plant and contains chemical compounds that have biological activity, such as flavonoids, carotenoids, and limonoids. The present study aimed to determine the effect of citrus leaf extract (Citrus hystrix) on the mortality of the larva Culex quinquefasciatus. The method used in the current study was a laboratory experimental study method with the experimental design using a completely randomized design. The research plot carried out was the rearing larvae of Culex quinquefasciatus, the manufacture of citrus leaf extract, the manufacture of larvicidal material, and the observation phase after treatment. Extract N-hexane omplet name hystrix leaf has high toxicity because it contains essential oils, flavonoids, alkaloids, terpenoids, saponins, and Limonoids. The Optimal concentration of N-hexane extracts of citrus leaves that lead to larval mortality of Culex quinquefasciatus in vitro amounted to 93.33% on 4 hours of observation with a concentration of 4000 ppm. Extract N-hexane lime leaf has a larvicidal effect on the larva Culex quinquefasciatus in vitro.

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Potensi Ekstrak Karamunting (Melastoma malabathricum L.) sebagai Insektisida Nabati untuk Mengendalikan Ulat Grayak (Spodoptera litura F.)

Agrotekma Jurnal Agroteknologi dan Ilmu Pertanian ◽

10.31289/agr.v4i1.2806 ◽

2019 ◽

Vol 4 (1) ◽

pp. 28

Author(s):

Kartina Kartina ◽

Shulkipli Shulkipli ◽

Mardhiana Mardhiana ◽

Saat Egra

Keyword(s):

Spodoptera Litura ◽

Leaf Extract ◽

Feeding Activity ◽

Larval Mortality ◽

Wild Plant ◽

Botanical Insecticides ◽

Botanical Insecticide ◽

Melastoma Malabathricum ◽

Randomized Design ◽

Completely Randomized Design

<h1>Spodoptera litura F. is one of the important pests in Horticulture commodities which can cause damage 80% and up to 100%. Karamunting (Melastome malabathricum) is a wild plant that grows in Tarakan City. This plant is known to contain secondary metabolites which are thought to have the potential to control pests. This study aims to determine the potential of Karamunting extract as a botanical insecticide material to control armyworms. This study uses a completely randomized design (CRD) method with 1 treatment factor, namely the concentration of Karamunting leaf extract. Potential extracts as botanical insecticides were tested by dye method using 4 concentrations, namely 0 (control); 500; 1000; 2000; 4000; and 8000 ppm. The results showed that Karamunting leaf extract was able to control armyworm pests. Giving extracts of 8000 ppm (P5) in feed caused the highest decrease in eating activity up to 41,2%, larval mortality up to 85%, and the highest mortality rate after 7 days of observation. Decreased feeding activity and larval mortality were thought to be influenced by the presence of phenol compounds, fatty acids, terpenoids, sterols and alkaloids identified in the extract. Thus, Karamunting leaf extract has the potential to be developed as an active ingredient in botanical insecticides to control armyworm.<strong></strong></h1>

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Uji Ekstrak Daun Pepaya (Carica Papaya) Terhadap Larva Plutella xylostella (Lepidoptera: Plutellidae)

Agrologia ◽

10.30598/a.v3i2.251 ◽

2018 ◽

Vol 3 (2) ◽

Cited By ~ 1

Author(s):

Victor G Siahaya ◽

Ria Y Rumthe

Keyword(s):

Plutella Xylostella ◽

Leaf Extract ◽

Carica Papaya ◽

Larval Mortality ◽

Lower Surface ◽

Severe Damage ◽

Plant Pests ◽

Randomized Design ◽

Completely Randomized Design ◽

Contact Poison

Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the major pests on chinese cabbage (Brassica pekinensis). It damage the 2-6 week old plants. Larvae damage crops by biting, chewing and then eating the lower surface of the leaves. The leaves will become transparent white, severe damage leaves behind only leaf bone. Papaya leaf (Carica papaya) could be used as a pesticide for crops because it contains substances that can inhibit eating even to kill insects pests. The purpose of this study was to verify the efficacy of the papaya leaf extract against Plutella xylostella. The study was conducted in the laboratory of Plant Pests Faculty of Agriculture, Pattimura University. The design used was a completely randomized design with four papaya leaf extract concentration at 3 instar larvae through the contact and stomach poison test. Observations were made on the early symptoms of poisoning, the percentage of larval mortality, and further Plutella xylostella stadium. The results showed that the highest mortality in the treatment of stomach and contact poison was occured at a concentration of 40 g/100 ml of water by 100%, while the lowest mortality at a concentration of 10 g / 100 ml of water, respectively by 35% and 20%.

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Daya Hambat Ekstrak Daun Sirsak, Kirinyuh, dan Rimpang Lengkuas terhadap Pertumbuhan Koloni Colletotrichum acutatum

Jurnal Agro ◽

10.15575/1373 ◽

2017 ◽

Vol 4 (2) ◽

pp. 80-89

Author(s):

Ida Hodiyah ◽

Elya Hartini ◽

Amir Amilin ◽

Moch Fauzian Yusup

Keyword(s):

Incubation Period ◽

Research Design ◽

Leaf Extract ◽

Colletotrichum Acutatum ◽

Randomized Design ◽

Completely Randomized Design

Several plants that are potentially used as bio-pesticides are soursop, siam weed, and galangal. The research objective was to find out the effectiveness of leaf extract of soursop and C. odorata, and extract of galangal rhizome in inhibiting the growth of Colletotrichum acutatum colonies, causing antracnose on chilli, in in vitro. The experiment was conducted in the laboratory of Agriculture Faculty, Universitas Siliwangi Tasikmalaya from July until August 2016. The research design used was a completely randomized design consisted of nine treatments and three replications. The treatments were A ( control); B (soursop leaf extract, 0,5%); C (soursop leaf extract, 1%); D (C. odorata leaf extract, 0,5 %); E (C. odorata leaf extract 1%); F (galangal rhizome extract 0,5%); G (galangal rhizome extract 1%); H (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 0,5%; and I (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 1%). The results showed that the mixture of each 1% soursop leaf extract, C.odorata leaf extract and galangal rhizome extract was effective in inhibiting the growth of C. acutatum colonies at 7 and 14 days incubation period by 66,19% and 69,94% respectively. The three extracts are potentials as anti-fungus of C. acutatum.

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Evaluation of efficacy of false yam (Icacina oliviformis) as surface protectant against rot pathogens of white yam (Dioscora rotundata Poir)

Ghana Journal of Agricultural Science ◽

10.4314/gjas.v54i1.1 ◽

2019 ◽

Vol 54 (1) ◽

pp. 1-9

Author(s):

E. N.K. Sowley ◽

F. Kankam ◽

R. M. Nsarko

Keyword(s):

Leaf Extract ◽

Tap Water ◽

Structural Features ◽

Leaf Extracts ◽

Randomized Design ◽

White Yam ◽

Completely Randomized Design ◽

In Vitro Tubers ◽

Tuber Rot

Rots in yam tubers are the dominant cause of postharvest losses. Therefore, studies were conducted to identify the rot-causing organisms in yam and to evaluate the efficacy of false yam (Icacina oliviformis) plant extracts as surface protectants in the storage of yam. The experiment was laid out in a completely randomized design (CRD) design with five treatments (fruit, root and leaf extract of false yam, Mancozeb and tap water) replicated three times. Two concentrations (50% and 100%) of each extract were also tested. Species were identified based on the structural features, the characteristics and properties of the spore and mycelium. A spore suspension of yam rot fungi was sprayed on healthy yam tubers that had been pretreated with the extracts. Results showed that fungi isolated from rotted yams were Aspergillus niger, A. flavus and Penicillium sclerotigenum. Leaf extracts (both 50% and 100%) had the highest growth inhibitions on all the three fungi isolated in vitro. Tubers treated with root and leaf extracts of false yam had a decreased tuber rot lengths of 1.80 mm2 and 2.17 mm2, respectively. The leaves and roots of false yam can be used as a surface protectant of yam in place of Mancozeb. Keywords: Icacina oliviformis; Dioscora rotundata; Mancozeb; Pathogenicity; Aspergillus spp.

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INHIBITOR PERTUMBUHAN Pyricularia oryzae DENGAN EKSTRAK CAIR DAUN PRONOJIWO (Euchresta horsfeildii) SECARA IN VITRO

STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa ◽

10.36456/stigma.vol10.no2.a1035 ◽

2018 ◽

Vol 10 (02) ◽

Author(s):

Astini Astini ◽

Tatang Sopandi

Keyword(s):

Experimental Study ◽

Leaf Extract ◽

Inhibition Zone ◽

Pyricularia Oryzae ◽

Liquid Extract ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

Inhibitory Power

Has been done research that aims to test the inhibitory power of pronojiwo (Euchresta horsfieldii) leaf extract to the fungus P.oryzae. the experimental study was conducted using a completely randomized design with 9 concentrations of liquid extract of pronojiwo leaf and 1 control. Ie the concentration of each pepeated 4 times and in duplo. Application is done by the method of well by means of liquid extract dripped on the diffusion well, dilusion method by means of liquid extract mixed on the media PDA, method of calculating the amount of fungus conidia by means of liquid extract mixed with media PDB, to determine the inhibition zone of the fungus, the growth of the diameter of the fungus, the number of living conidia. Observations were made after incubation for 7 days. The results showed that liquid extract pronojiwo leaves can inhibit and reduce the growth of fungus P.oryzae, at a concentration of 6,25% with a resistor zone result of 0,9 mm, a growth diameter percentage of 71,2%, and may reduce by 2,9 conidia/ml. Keywords : pronojiwo leaves, growth, reduce , fungus P.oryzae

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Biolarvacide of Culex quinquefasciatus

Biota ◽

10.20414/jb.v13i1.234 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Rian Oktiansyah ◽

Anggun Wicaksono ◽

Fahmy Armanda ◽

Amin Nurokhman ◽

Ummi Hiras Habisukan ◽

...

Keyword(s):

Analysis Of Variance ◽

Culex Quinquefasciatus ◽

Carica Papaya ◽

Larval Mortality ◽

Psidium Guajava ◽

Mimosa Pudica ◽

Randomized Design ◽

Leaf Powder ◽

Completely Randomized Design ◽

Guava Leaves

Biolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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PERBANDINGAN DAYA HAMBAT BAKTERI Pseudomonas fluorescens ASAL TOMAT, KEDELAI, DAN JAGUNG TERHADAP Ralstonia solanacearum SECARA IN VITRO

Agrotek ◽

10.30862/agt.v5i6.602 ◽

2018 ◽

Vol 5 (6) ◽

Author(s):

Aco Roni Kirihio ◽

Ivonne Fitria Mariay ◽

Cipta Meliala

Keyword(s):

Pseudomonas Fluorescens ◽

Filter Paper ◽

Ralstonia Solanacearum ◽

Randomized Design ◽

Completely Randomized Design

<em>Inhibition of Pseudomonas fluorescens isolates the origin of tomato, soybean and corn against Ralstonia solanacearum tested using a completely randomized design (CRD). P. fluorescens growth was measured at King's B medium by way of suspension antagonist put on filter paper of 0.5 cm in diameter. Inhibition of P. fluorescens is done by placing the antagonist suspension of 0.5 cm diameter filter paper on NA media that has been deployed R. solanacearum. The results showed that: (a) the growth of P. fluorescens origin of tomato, soybean and corn on King's B media were not significantly different, (b) the inhibition of P. fluorescens isolates against R. solanacearum not significantly different and, (c) the inhibition of isolates P. fluorescens origin of tomato, soybean and corn against R. solanacearum in vitro relatively strong</em>

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PENGARUH EKSTRAK DAUN MIMBA TERHADAP PERKEMBANGAN HAMA Callosobruchus chinensis L. (Coleoptera : Bruchidae)

AGRICA ◽

10.37478/agr.v1i2.306 ◽

2019 ◽

Vol 1 (2) ◽

pp. 32-37

Author(s):

Yustina M.S.W Puu ◽

Hildegardis Nalti Nansi

Keyword(s):

Mung Bean ◽

Leaf Extract ◽

Callosobruchus Chinensis ◽

Spawning Activity ◽

Green Beans ◽

Leaf Extracts ◽

Randomized Design ◽

Completely Randomized Design ◽

Contact Poison ◽

Neem Leaf Extract

Callosobruchus Chinensis is a pest that damages mung bean seeds in storage and causes damage to both the quality and quantity of seeds. This study aims to determine the effectiveness of neem leaf extracts in suppressing the development of the Callosobruchus Chinensis pest as one of the postharvest pests in the commodity green beans. This research conducted at the Laboratory of the Faculty of Agriculture, University of Flores, by using a Completely Randomized Design (CRD) with five treatments and three replications. The concentration of neem leaf extract treatment is 0 ml / l, 25 ml / l, 30 ml / l, 35 ml / l, and 40 ml / l. The results showed that the concentration of neem leaf extract 40 ml / l caused mortality of C. Chinensis imago as contact poison by 99% and nerve poison by 47%. While the effectiveness of the limb leaf extract on spawning activity was 86%.

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