scholarly journals Leptospirosis in free-living capybaras (Hydrochaeris hydrochaeris) from a university campus in the city of Araras in São Paulo, Brazil

2020 ◽  
Vol 41 (1) ◽  
pp. 159
Author(s):  
Daniela Dib Gonçalves ◽  
Karoline Franciane Cardoso Lopes ◽  
Roberta Torres Chiderolli ◽  
Bruno Rodrigues Sampieri ◽  
Vlamir José Rocha ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Animal Species ◽  
Sao Paulo ◽  
São Paulo ◽  
University Campus ◽  
Serum Samples ◽  
Free Living ◽  
Leptospira Spp ◽  
The City ◽  
Hydrochaeris Hydrochaeris

The capybara (Hydrochaeris hydrochaeris L. 1766) is the largest existing rodent in the world. This animal species, being synanthropic, may serve as a transmitter of different diseases and parasitic infections in animals and humans as well. Leptospirosis is a cosmopolitan infectious disease with a high prevalence in tropical and subtropical regions that can affect humans and other domestic and wild animals. Due to the absence of regional data and the importance of this animal species in transmitting diseases to animals and humans, the aim of this study was to analyze DNA and anti-Leptospira spp. antibodies in free-living capybaras (Hydrochaeris hydrochaeris) from a university campus in the city of Araras in São Paulo, Brazil. A total of 31 capybaras (Hydrochaeris hydrochaeris) were captured for collecting their blood samples. The collected sera were analyzed using the microscopic agglutination test (MAT). For the detection of Leptospira spp. DNA, the serum samples were used to extract genomic DNA for the nested-PCR analysis. Out of the 31 serum samples, 29 (93.55%) were reactive for MAT, with titers ranging from 25 to 400. The antibody could be identified against the most probable serovar in 26 (89.65%) samples, namely: Grippotyphosa (69.23%), Autumnalis (26.92%), and Bratislava (3.85%). Presence of Leptospira via nested-PCR was found only in 3.22% of serum samples. This study revealed the presence of DNA and anti-Leptospira spp. antibodies in free-living capybara. Characterization of these animals as possible carriers and disseminators of the etiological agent in the environment is necessary for identification of infection in other animals and campus visitors.

scholarly journals Detection of anti-Toxoplasma gondii antibodies in wild free-living birds and mammals from the northwest region of São Paulo state, Brazil

2021 ◽  
Vol 58 ◽  
pp. e176683
Author(s):  
Lilian Castiglioni ◽  
Luiz Paulo Nogueira Aires ◽  
Vinícius Matheus Ferrari ◽  
Fernando Henrique Antunes Murata ◽  
Herbert Sousa Soares ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Animal Species ◽  
Sao Paulo ◽  
São Paulo ◽  
Wild Animals ◽  
Free Living ◽  
Paulo State ◽  
Two Samples ◽  
São Paulo State ◽  
Northwest Region

Toxoplasmosis is a protozoonosis caused by an obligate intracellular parasite named Toxoplasma gondii, which can infect humans and a large number of homeothermic animal species with worldwide distribution. The present study aimed to detect anti T. gondii antibodies from serological samples of free living wild animals from the northwest region of São Paulo state, Brazil. Thirty-two samples (eight from birds and 24 from mammals) were analyzed by the modified agglutination test (MAT) using 5 cut-off points for birds and 25 for mammals. Seropositivity was observed in 25% (2/8) of birds, including the species Rupornis magnirostris (roadside hawk) and Caracara plancus (southern caracara), and 29.2% (7/24) animals were seropositive among mammals, including one hoary fox (Lycalopex vetulus), two maned wolves (Chrysocyon brachyurus), one black howler monkey (Alouatta caraya), two crab-eating foxes (Cerdocyon thous) and one gray brocket deer (Mazama gouazoubira). The results obtained with the present study indicate the exposure to T. gondii of free-living wild animals from the northwest region of São Paulo state and, therefore, that they probably play a role in the transmission and maintenance of T. gondii in the environment they inhabit. Thus, identification of the infection in several animal species in the region indicates the environmental contamination of the area. Studies of this nature may help to understand the importance of the prevention and control of this disease in Brazil.

scholarly journals Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques

2010 ◽  
Vol 19 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Fernanda Sartori Lima de Godoi ◽  
Sandra Mayumi Nishi ◽  
Hilda Fátima de Jesus Pena ◽  
Solange Maria Gennari
Keyword(s):  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Natural Infection ◽  
Columba Livia ◽  
Sao Paulo ◽  
Molecular Techniques ◽  
Southeastern Brazil ◽  
Complete Agreement ◽  
São Paulo ◽  
Free Living

This study aimed to diagnose experimental and natural Toxoplasma gondii infection in pigeons (Columba livia) by serological, biological and molecular techniques. Twelve pigeons, free of infection, were inoculated with 50 sporulated oocysts of T. gondii (VEG sample) and four remained uninfected controls. Four birds (three infected and one control) were euthanized at 15, 30, 45 and 60 days post-infection (dpi), and their tissues were used to perform a bioassay in mice and nested-PCR using B1 gene as target. Blood was obtained weekly and it was tested for the presence of anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT). Seven (58.3%) out of 12 inoculated pigeons were positive by serological techniques and titers ranged between 1:40 and 1:5120 by MAT and between 1:512 and 1:4096 by IFAT. Complete agreement was seen between the results obtained by serological techniques and nested-PCR in seven positive birds. In the bioassay in mice, five (41.7%) out of 12 pigeons inoculated were positive to T. gondii. Only one pigeon died at 23 dpi due to toxoplasmosis. A second study with free-living pigeons was performed for detection of anti-T. gondii antibodies. Birds were captured in the municipalities of São Paulo, Ibiúna and Sorocaba, São Paulo State, Southeastern Brazil. All 126 free-living birds were negative to anti-T. gondii antibodies by MAT (titer < 1:5). Bioassays were performed in mice with tissues from all captured birds and T. gondii was not isolated in any pigeon.

scholarly journals Fifteen years of human leptospirosis in São Paulo, Brazil

2015 ◽  
Vol 2 (1) ◽  
pp. 56 ◽  
Author(s):  
Roberta M. Blanco ◽  
Eliete C. Romero
Keyword(s):  
Public Health ◽  
Age Groups ◽  
Sao Paulo ◽  
São Paulo ◽  
Serum Samples ◽  
Public Health Importance ◽  
Pathogenic Leptospira ◽  
Human Leptospirosis ◽  
Leptospira Spp ◽  
Seasonal Analysis

Objective: Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. The aim of this study was to report theincidence of leptospirosis from 1998 to 2012 in the state of São Paulo, Brazil to show the importance of human leptospirosis andto describe some epidemiological characteristics.Methods: From January 1998 to December 2012, sera from patients with suspected leptospirosis were analyzed. The microscopicagglutination test (MAT) was used for serological investigations and MLST, serotyping and PFGE methods for the identificationof leptospires. The descriptive seasonal analysis was performed with Excel Microsoft version 2007. Pearson’s correlation wasused to assess the association between rainfall and the number of cases.Results: Among 22,795 serum samples, 2,430 cases of leptospirosis were laboratory confirmed, giving an average incidence rateof 1.35/100,000 inhabitants. Of these patients, 2,032 (83.62%) were male with a predominance in the age groups of 21-50 years.The highest incidence and rainfall were from December to April. There was correlation between the rainfall and the number ofcases. Icterohaemorrhagiae was the predominant serogroup.Conclusions: This study shows that leptospirosis is a seasonal disease in São Paulo with most cases occurring during the rainyseason, and thus, will continue to be a disease of public health importance. 

scholarly journals Zoonosis associated to Rattus rattus and the impacts of the public actions to control the species

2016 ◽  
Vol 83 (0) ◽  
Author(s):  
Lilian dos Santos Babolin ◽  
Maria Jeovânia Freire de Almeida-Silva ◽  
Marcos Roberto Potenza ◽  
Claudia Del Fava ◽  
Vanessa Castro ◽  
...  
Keyword(s):  
Rattus Rattus ◽  
Winter Season ◽  
Control Program ◽  
Sao Paulo ◽  
Effective Control ◽  
São Paulo ◽  
Rodent Control ◽  
Serum Samples ◽  
Black Rat ◽  
The City

ABSTRACT: The occurrence of the black rat, Rattus rattus , in major Brazilian cities has increased in the recent years. To evaluate if the efforts of public rodent control have been reaching the species in the city of São Paulo, a survey was conducted in flooding areas using live trapping before and after implementation of the control program. Captures were carried out from 2009 to 2011, and animals were evaluated for their risk of zoonosis transmission. The number of R. rattus captured after control did not differ from the number of animals captured before the control program in the Spring/Summer season, but, in the Autumn/Winter season, effective control was observed. Low infestations of Ctenocephalides felis (Siphonaptera) and Echinolaelaps echidninus (Acarina) were observed, and antibodies against Leptospira spp. were detected in just 1 of 65 serum samples. Ten out of 18 serum samples were sororeactive for Vaccinia virus, suggesting contact between R. rattus and the pathogen Calodium hepaticum (= Capillaria hepaticum ) (Nematoda) was found in the liver of 73.4% of captured R. rattus. In conclusion, R. rattus may not be effectively controlled by the rodent Control Program in the city of São Paulo, and represents a threat to human health, justifying permanent environmental management and educational programs.

Seasonal Variation of Suicide in the City of São Paulo, Brazil, 1996–2010

Crisis ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Daniel Hideki Bando ◽  
Fernando Madalena Volpe
Keyword(s):  
Seasonal Pattern ◽  
Total Sample ◽  
Sao Paulo ◽  
Seasonal Patterns ◽  
São Paulo ◽  
Health Statistics ◽  
Males And Females ◽  
June July ◽  
The City ◽  
Over Time

Background: In light of the few reports from intertropical latitudes and their conflicting results, we aimed to replicate and update the investigation of seasonal patterns of suicide occurrences in the city of São Paulo, Brazil. Methods: Data relating to male and female suicides were extracted from the Mortality Information Enhancement Program (PRO-AIM), the official health statistics of the municipality of São Paulo. Seasonality was assessed by studying distribution of suicides over time using cosinor analyses. Results: There were 6,916 registered suicides (76.7% men), with an average of 39.0 ± 7.0 observed suicides per month. For the total sample and for both sexes, cosinor analysis estimated a significant seasonal pattern. For the total sample and for males suicide peaked in November (late spring) with a trough in May–June (late autumn). For females, the estimated peak occurred in January, and the trough in June–July. Conclusions: A seasonal pattern of suicides was found for both males and females, peaking in spring/summer and dipping in fall/winter. The scarcity of reports from intertropical latitudes warrants promoting more studies in this area.

scholarly journals AEROSOLS OBSERVATIONS BY AN ELASTIC LIDAR SYSTEM OVER THE CITY OF SÃO PAULO, BRAZIL

2001 ◽  
Vol 32 ◽  
pp. 407-408
Author(s):  
E. LANDULFO ◽  
A. PAPAYANNIS ◽  
A. ZANARDI DE FREITAS ◽  
M.P.P.. M. JORGE ◽  
N.D. VIEIRA JÚNIOR
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Lidar System ◽  
The City

scholarly journals Porcine Circovirus 3a Field Strains in Free-Living Wild Boars in Paraná State, Brazil

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1634
Author(s):  
Tatiana Carolina Gomes Dutra de Souza ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Raquel Arruda Leme ◽  
Gisele da Silva Porto ◽  
...  
Keyword(s):  
Animal Species ◽  
Porcine Circovirus ◽  
Whole Genome ◽  
Living Animal ◽  
Pcr Assay ◽  
Serum Samples ◽  
Viral Dna ◽  
Free Living ◽  
Wild Boars ◽  
The World

Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-3 genome. The three full-length sequences obtained were grouped in PCV-3 clade “a”, and the sequences exhibited 100% nucleotide similarity among them. The PCV-3 field strains of this study showed nucleotide and amino acid similarities of 98.5–99.8% and 98.8–100%, respectively, with whole-genome PCV-3 sequences from around the world.

scholarly journals The Impact of COVID-19 on Urban Agriculture in São Paulo, Brazil

2021 ◽  
Vol 13 (11) ◽  
pp. 6185
Author(s):  
André Ruoppolo Biazoti ◽  
Angélica Campos Nakamura ◽  
Gustavo Nagib ◽  
Vitória Oliveira Pereira de Souza Leão ◽  
Giulia Giacchè ◽  
...  
Keyword(s):  
Urban Agriculture ◽  
Technical Assistance ◽  
Community Gardens ◽  
Sao Paulo ◽  
Food Chains ◽  
São Paulo ◽  
Sales Channels ◽  
Paulo State ◽  
The Impact ◽  
The City

During the initial months of the COVID-19 pandemic, farmers worldwide were greatly affected by disruptions in the food chain. In 2020, São Paulo city experienced most of the effects of the pandemic in Brazil, with 15,587 deaths through December 2020. Here, we describe the impacts of COVID-19 on urban agriculture (UA) in São Paulo from April to August 2020. We analyzed two governmental surveys of 2100 farmers from São Paulo state and 148 from São Paulo city and two qualitative surveys of volunteers from ten community gardens and seven urban farmers. Our data showed that 50% of the farmers were impacted by the pandemic with drops in sales, especially those that depended on intermediaries. Some farmers in the city adapted to novel sales channels, but 22% claimed that obtaining inputs became difficult. No municipal support was provided to UA in São Paulo, and pre-existing issues were exacerbated. Work on community gardens decreased, but no garden permanently closed. Post COVID-19, UA will have the challenge of maintaining local food chains established during the pandemic. Due to the increase in the price of inputs and the lack of technical assistance, governmental efforts should be implemented to support UA.

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