Isolation and identification of common food-borne bacteria from spicy puffed rice and determination of their antibiotic resistance profile

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Md Rahman
Keyword(s):  
Antibiotic Resistance ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
Common Food ◽  
Antibiotic Resistance Profile ◽  
Food Borne ◽  
Puffed Rice

scholarly journals Antibiotic resistance profiles of Staphylococci of animal and human origin

2019 ◽  
Vol 3 (1) ◽  
pp. 28-34
Author(s):  
Saoussene Smaali
Keyword(s):  
Antibiotic Resistance ◽  
Reference Method ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Resistant Bacteria ◽  
Preventive Strategy ◽  
Isolation And Identification ◽  
Antibiotic Resistance Profile ◽  
Biological Liquids

The emergence of multidrug-resistant bacteria in animals and their products has highlighted the considerable value of the potential transfer of resistance to the human population via the food chain. The objective of this work was to study the antibiotic resistance profile of staphylococci of human and animal origin and to compare the two profiles in order to define an effective therapeutic and preventive strategy. This work was carried out on a set of 97 strains of staphylococci isolated from animal and human biological liquids. The isolation and identification was done by conventional methods. The determination of the minimum inhibitory concentrations (MIC) was studied by the reference method (dilution in agar medium) and VITEK 2® with respect to eight (8) antibiotic molecules. The results were interpreted according to the ACFSM criteria (2001). The results showed higher resistance of human bacteria to penicillin (72.22% vs. 30.23%) erythromycin (50% vs. 18.6%) and gentamicin (33.33% vs. 9.3%) compared to animal bacteria. On the other hand, the resistance to fosfomycin of staphylococci of animal origin was higher (60.46%) compared to human strains (38.89%). Indeed, it seems that vancomycin and rifampicin are the antibiotics of choice against these pathogenic strains in veterinary and human medicine.

scholarly journals Prevalence and antibiotic resistance profile of Listeria spp. associated with seafoods from fish catchment areas in Kerala, India

2021 ◽  
Vol 14 (3) ◽  
pp. 777-783
Author(s):  
K. Vrinda Menon ◽  
B. Sunil ◽  
C. Latha
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
Fresh Fish ◽  
Antibiotic Resistance Profile ◽  
Catchment Areas ◽  
Antibiotic Sensitivity Test ◽  
Serotype 4B

Background and Aim: Listeria monocytogenes is a ubiquitous, intracellular pathogen which has been implicated as a cause of several foodborne outbreaks. This study aimed to generate information on the prevalence and antibiotic resistance profile of Listeria species isolated from seafood. Materials and Methods: A total of 400 samples of fresh fish, 100 samples of dry fish and 200 samples each of crustaceans and mollusks were collected from the fish catchment areas. All the samples were subjected to isolation and identification of Listeria spp. by two-step enrichment in UVM broth and plating on selective agar media (PALCAM) and then subjected to molecular characterization. L. monocytogenes isolates obtained during the study were subjected to serotyping by multiplex polymerase chain reaction. The isolates were also subjected to antibiotic sensitivity test. Results: The prevalence of L. monocytogenes in seafoods in the present study was 0.55%. The isolates of L. monocytogenes were found to possess all virulence genes, namely, iap, hlyA, actA, prfA, plcA, and inlA. All the isolates belonged to serotype 4b. The occurrence of Listeria innocua was found to be more and was detected in 16.77% of seafoods samples. Antibiotic sensitivity test revealed that all isolates were resistant to cefixime but were sensitive to almost all other commonly used antibiotics. Conclusion: The presence of Listeria spp. in raw seafood samples augments the need for implementation of good hygienic practices during the handling and processing of seafoods to safeguard the health of the consumers.

scholarly journals Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kamana Bantawa ◽  
Shiv Nandan Sah ◽  
Dhiren Subba Limbu ◽  
Prince Subba ◽  
Arjun Ghimire
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Resistance Patterns ◽  
Meat Samples

Abstract Objective Food-borne pathogens are a major cause of illnesses, death and expenses. Their occurrence in meat and other food is considered a global health problem. The burden of food-borne disease is increasing due to antimicrobial resistance which represents a greater risk of treatment failure. However, very little is known about the antibiotic resistance profile of food-borne pathogens in Nepal. This study was conducted to examine the antibiotic resistance profile of common food-borne bacterial pathogens isolated from raw meat sold in Nepal. A total of 83 meat samples were collected from the market and analyzed. Results The prevalence of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella, and Vibrio were 68%, 53%, 35%, 6%, and 6% respectively. The resistance of Salmonella was most frequently observed to amoxicillin (100%), tetracycline (24%), chloramphenicol (11%), and nalidixic acid (11%). S. aureus was resistant to amoxicillin (100%) followed by tetracycline (63%), nalidixic acid (17%), and cefotaxime (13%) respectively. Vibrio isolates resisted amoxicillin (100%), tetracycline (40%) and chloramphenicol (20%). Shigella expressed the highest resistance to amoxicillin (100%), followed by chloramphenicol (80%), tetracycline (60%) and nalidixic acid (20%). E. coli exhibited the highest resistance to amoxicillin (100%), followed by tetracycline (93%), nalidixic acid (25%) and cefotaxime (19%).

scholarly journals Comparison of Antibiotic Resistance Profile of Escherichia coli between Pristine and Human-Impacted Sites in a River

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 575
Author(s):  
Emi Nishimura ◽  
Masateru Nishiyama ◽  
Kei Nukazawa ◽  
Yoshihiro Suzuki
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistant Bacteria ◽  
Downstream Site ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Upstream Site ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Significant Difference

Information on the actual existence of antibiotic-resistant bacteria in rivers where sewage, urban wastewater, and livestock wastewater do not load is essential to prevent the spread of antibiotic-resistant bacteria in water environments. This study compared the antibiotic resistance profile of Escherichia coli upstream and downstream of human habitation. The survey was conducted in the summer, winter, and spring seasons. Resistance to one or more antibiotics at upstream and downstream sites was on average 18% and 20%, respectively, and no significant difference was observed between the survey sites. The resistance rates at the upstream site (total of 98 isolated strains) to each antibiotic were cefazolin 17%, tetracycline 12%, and ampicillin 8%, in descending order. Conversely, for the downstream site (total of 89 isolated strains), the rates were ampicillin 16%, cefazolin 16%, and tetracycline 1% in descending order. The resistance rate of tetracycline in the downstream site was significantly lower than that of the upstream site. Furthermore, phylogenetic analysis revealed that many strains showed different resistance profiles even in the same cluster of the Pulsed-Field Gel Electrophoresis (PFGE) pattern. Moreover, the resistance profiles differed in the same cluster of the upstream and the downstream sites. In flowing from the upstream to the downstream site, it is plausible that E. coli transmitted or lacked the antibiotic resistance gene.

scholarly journals ANTIBIOTIC RESISTANCE PROFILING OF DAIRY WASTEWATER DEGRADING NATIVE EFFICIENT MICROBIAL ISOLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 145
Author(s):  
Anupama Bhardwaj ◽  
Jagtar Singh ◽  
Sonia Chaman ◽  
Amit Joshi
Keyword(s):  
Antibiotic Resistance ◽  
Oxygen Demand ◽  
Dairy Wastewater ◽  
Organic Load ◽  
Diffusion Method ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Surrounding Environment ◽  
Before And After ◽  
Microbial Isolates

Objective: The objective of this study is to make sure biotreatment process used for treatment of dairy wastewater (DWW) is safe for human and its surrounding environment; microbes were evaluated for their antibiotic resistance profile against commonly prescribed antibiotics. Methods: Microbes were isolated using spread plating and streaking method and used to treat DWW. Reduction in organic load in DWW was determined by comparing physicochemical parameters (PCP) of DWW before and after treatment process. After selection of efficient microbial isolates, they were evaluated for their antibiotic resistance profile using antibiotic disc diffusion method. Results: In this work, 53 microbes were isolated from DWW, and these microbial isolates were screened for DWW degradation capacity by analyzing PCP. Four microbial isolates E3, E5, E11 (bacterial isolates) and F5 (fungal isolate) showed highest reduction in chemical oxygen demand (COD), biological oxygen demand (BOD), and dissolved oxygen (DO) were selected for profound degradation of DWW under optimized conditions. Efficient four microbial isolates individually performed better under anaerobic conditions by showing maximum reduction 84%, 75%, and 77% in COD, BOD, and DO, respectively. After 72 hrs of antibiotic susceptibility testing, E3 strain had shown 100%, E5 90%, E11 70%, and F5 80% susceptibility to antibiotics. Conclusion: The present study concluded that four microbial isolates had the potential of reducing the organic load of DWW along with lessor or negligible adverse effect on human or its surrounding environment and they appear to be most promising strains for treatment of DWW. 

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