Evaluation of anti-diabetic and anti-inflammatory activities of Fenugreek (Trigonella foenum-graecum) seed extracts on Albino Wistar Rats

Some previous studies mentioned that papaya seed extract has anti-inflammatory activity because it contains polyphenols including phenolic and flavonoid acids. Polyphenols of the papaya seed extracts are known to inhibit the severity of periodontitis. It can prevent the release of excessive osteoclastogenesis which may promote bone resorption aggravating periodontitis. Hence, this study aims to determine the role of papaya seed extract in reducing the number of osteoclasts in rats with periodontitis. Twenty-eight male Wistar rats were divided into 4 groups of induced periodontitis using lipopolisakarida (LPS) Porphyromonas gingivalis (P. Gingivalis) and followed by wire ligature in “8” placed around the mandible mandibular incisor. The first group was given standard food without any papaya extract, while each rat in three other groups with periodontitis (P2, P3, P4) was given with Papaya seed extracts of 200 mg/kgBW, 300 mg/kgBW, and 400 mg/kgBW. Seven rats in one group were not induced by periodontitis and were fed with standard food as a control group. On the 30th day, the rat was sacrificed, and its transverse pieces of alveolar bone around the mandibular incisor were stained with Hematoxylin-Eosin. The data analyzed by one way ANOVA indicated a significant result. Thus, it is conclusive that extract of papaya seed ethanol inhibited osteoclastogenesis in Wistar rats induced with periodontitis.

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3,4,5-Trihydroxybenzoic acid attenuates ligature-induced periodontal disease in Wistar rats.

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523019666200206094335 ◽

2020 ◽

Vol 19 ◽

Author(s):

Ozkan Karatas ◽

Fikret Gevrek

Keyword(s):

Bone Loss ◽

Gallic Acid ◽

Wistar Rats ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Collagenase Activity ◽

Osteoblastic Activity ◽

Cell Counts ◽

Experimental Periodontitis ◽

Anti Inflammatory

Background: 3,4,5-Trihydroxybenzoic acid, which is also known as gallic acid, is an anti-inflammatory agent who could provide beneficial effects in preventing periodontal inflammation. The present study aimed to evaluate the anti-inflammatory effects of gallic acid on experimental periodontitis in Wistar rats. Alveolar bone loss, osteoclastic activity, osteoblastic activity, and collagenase activity were also determined. Methods: 32 Wistar rats were used in the present study. Study groups were created as following: Healthy control (C,n=8) group; periodontitis (P,n=8) group; periodontitis and 30 mg/kg gallic acid administered group (G30,n=8); periodontitis and 60 mg/kg gallic acid administered group (G60,n=8). Experimental periodontitis was created by placing 4-0 silk sutures around the mandibular right first molar tooth. Morphological changes in alveolar bone were determined by stereomicroscopic evaluation. Mandibles were undergone histological evaluation. Matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, bone morphogenetic protein (BMP)-2 expressions, tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and inflammatory cell counts were determined. Results: Highest alveolar bone loss was observed in the periodontitis group. Both doses of gallic acid decreased alveolar bone loss compared to the P group. TRAP-positive osteoclast cell counts were higher in the P group, and gallic acid successfully lowered these counts. Osteoblast cells also increased in gallic acid administered groups. Inflammation in the P group was also higher than those of C, G30, and G60 groups supporting the role of gallic acid in preventing inflammation. 30 and 60 mg/kg doses of gallic acid decreased MMP-8 levels and increased TIMP-1 levels. BMP levels increased in gallic acid administered groups, similar to several osteoblasts. Conclusion: Present results revealed an anti-inflammatory effect of gallic acid, which was indicated by decreased alveolar bone loss and collagenase activity and increased osteoblastic activity.

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Defatted Hydroethanolic Extract of Ammodaucus leucotrichus Cosson and Durieu Seeds: Antidiabetic and Anti-Inflammatory Activities

Applied Sciences ◽

10.3390/app10249147 ◽

2020 ◽

Vol 10 (24) ◽

pp. 9147

Author(s):

Imane Es-Safi ◽

Hamza Mechchate ◽

Amal Amaghnouje ◽

Anna Calarco ◽

Smahane Boukhira ◽

...

Keyword(s):

Wistar Rats ◽

Fasting Blood Glucose ◽

Diabetic Mice ◽

North African ◽

Glucose Levels ◽

The North ◽

Blood Glucose Levels ◽

Anti Inflammatory ◽

Hydroethanolic Extract ◽

Inflammatory Effect

The seeds of Ammodaucus leucotrichus Cosson and Durieu have been used in the North African Sahara as a traditional medicine to treat diabetes. The present study investigates the antidiabetic, antihyperglycemic, and anti-inflammatory properties of the defatted hydroethanolic extract of Ammodaucus leucotrichus (DHEAM). The antidiabetic and the antihyperglycemic studies were assessed on alloxan-induced diabetic with orally administered doses of DHEAM (100 and 200 mg/kg). At the same time, its anti-inflammatory propriety was evaluated by measuring edema development in the Wistar rats paw induced with carrageenan. Treatment of diabetic mice with DHEAM for four weeks managed their high fasting blood glucose levels, improved their overall health, and also revealed an excellent antihyperglycemic activity. Following the anti-inflammatory results, DHEAM exhibited a perfect activity. HPLC results revealed the presence of seven molecules (chlorogenic acid, 3-p-coumaroylquinic acid, gallic acid, ferulic acid, myricetin, quercetin, luteolin). This work indicates that the DHEAM has an important antidiabetic, antihyperglycemic, and anti-inflammatory effect that can be well established as a phytomedicine to treat diabetes.

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41. Anti-Inflammatory Effect of Honey Bee Venom on Wistar Rats Induced Poly Cystic Ovarian Syndrome by Estradiol Valerate

Toxicon ◽

10.1016/j.toxicon.2012.04.042 ◽

2012 ◽

Vol 60 (2) ◽

pp. 115-116

Author(s):

Mohammad Nabiuni ◽

Kazem Parivar ◽

Bahman Zeynali ◽

Azar Sheikholeslami ◽

Latifeh Karimzadeh

Keyword(s):

Honey Bee ◽

Wistar Rats ◽

Bee Venom ◽

Estradiol Valerate ◽

Anti Inflammatory ◽

Ovarian Syndrome ◽

Inflammatory Effect

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EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.16708 ◽

2017 ◽

Vol 9 (5) ◽

pp. 29

Author(s):

Iswariya S. ◽

Uma T. S.

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Aqueous Extract ◽

Seed Extract ◽

Inflammatory Activity ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Anti Inflammatory ◽

Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

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Staining Interactions of Bixa Orellana (Annatto) Seed Extracts with Tissues of Wistar Rats

Annals of Advanced Biomedical Sciences ◽

10.23880/aabsc-16000144 ◽

2019 ◽

Vol 2 (2) ◽

Author(s):

Fasogbon Samuel Ayobami

Keyword(s):

Column Chromatography ◽

Wistar Rats ◽

Blood Smear ◽

Wistar Rat ◽

Ethanol Solution ◽

Giemsa Stain ◽

Staining Reaction ◽

Distilled Water ◽

Bixa Orellana ◽

Seed Extracts

Introduction: Bixa orellana is an ancestral multi use plant popularly known as Achiote or lipstick tree in view of its reddish – orange dye on its seeds, Central and South American populations used these seeds to color their bodies and lips, B. orellana is the only species of Bixaceae family. Aim: The aim of this study was to stain the organs such as skin, liver, kidney, brain, intestines, and bone marrow of a wistar rats with solutions of Bixa orellana (Annatto) seed extracts. Methods: The study design was experimental research, the seeds of Bixa orellana were extracted in distilled water, absolute ethanol and acetone, using maceration methods and the fractions of the extracts were determined using column chromatography while preliminary, the solutions of the extracts were used to stain tissues listed above at varying pH to establish staining interactions of the crude extracts and fractions eluted from the column chromatography. Results: The most significant staining interaction were observed in ethanol solution of bixa extract, followed by acetone and distilled water and staining were also observed at column fraction(9-26), with background yellowish appearance in all the staining solutions which was contrast to controls (haematoxylin and eosin) stained sections and no staining reaction was observed in blood smear of wistar rat. The staining interactions of great quality was observed at pH 5.0 and 7.5, despite its staining ability it was neither superior to eosin nor a substitute to giemsa stain due to the yellowish background appearance and its complete inactivity on blood smear. Conclusion: Bixa orellana dye extracts can be grouped under compound dyes or neutral dyes due to its interaction at both pH 5.0 and 7.5 and cannot be used as substitute for Giemsa stain.

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Evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Enhydra fluctuans on male wistar rats

Journal of Applied Pharmaceutical Research ◽

10.18231/joapr.2021.9.1.37.42 ◽

2021 ◽

Vol 9 (1) ◽

pp. 1-7

Author(s):

Kishor Kumar Roy ◽

Md. Kabirul Islam Mollah ◽

Md. Masud Reja ◽

Dibyendu Shil ◽

Ranjan Kumar Maji

Keyword(s):

Wistar Rats ◽

Pain Threshold ◽

Ethanolic Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Hot Plate ◽

Plate Method ◽

Traditional System ◽

Male Wistar Rats ◽

Anti Inflammatory

Enhydra fluctuations an edible semi-aquatic vegetable plant are widely used in the traditional system of medicine. Ethanolic extract of Enhydra fluctuans was screened for analgesic & anti-inflammatory activity. Analgesic activity was evaluated by the hot plate method and anti-inflammatory activity was evaluated by formalin induce paw edema in Wistar rats (male). Ethanolic extract dose of 300 mg/kg reduce formalin induce paw inflammation and also increased the pain threshold in rats evidenced by hot plate method. The experimental results concluded that Enhydra fluctuans have significant analgesic and anti-inflammatory activity may due to flavonoid and phenolic compound content.

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Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats

Scientific Reports ◽

10.1038/s41598-021-98248-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Larissa de Brito Medeiros ◽

Susana Paula Almeida Alves ◽

Rui José Branquinho de Bessa ◽

Juliana Késsia Barbosa Soares ◽

Camila Neves Meireles Costa ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Gut Microbiota ◽

Wistar Rats ◽

High Fat Diets ◽

Serum Biochemical ◽

Anti Inflammatory ◽

Diet Intake ◽

Fat Diets ◽

Gut Microbiota Composition

AbstractThis study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct effects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amount of fat; HVF-20% of hydrogenated vegetable fat (industrial); and RUM-20% of ruminant fat (natural). After 53 days of treatment, serum biochemical markers, fatty acid composition of liver, heart and adipose tissue, histology and hepatic oxidative parameters, as well as gut microbiota composition were evaluated. HVF diet intake reduced triglycerides (≈ 39.39%) and VLDL levels (≈ 39.49%). Trans-fatty acids levels in all tissue were higher in HVF group. However, RUM diet intake elevated amounts of anti-inflammatory cytokine IL-10 (≈ 14.7%) compared to CONT, but not to HVF. Furthermore, RUM intake led to higher concentrations of stearic acid and conjugated linoleic acid in all tissue; this particular diet was associated with a hepatoprotective effect. The microbial gut communities were significantly different among the groups. Our results show that ruminant fat reversed the hepatic steatosis normally caused by high fat diets, which may be related to the remodelling of the gut microbiota and its anti-inflammatory potential.

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