scholarly journals Screening of Heat Stress Tolerant in Early Stage of Wheat Seedling using Morphological Parameters

2022 ◽  
pp. 667-672
Author(s):  
Pooja Sharma ◽  
Raj Singh ◽  
Monika Dahiya ◽  
Vikas Kumar ◽  
Amit Kumar ◽  
...  
Keyword(s):  
Heat Stress ◽  
Early Stage ◽  
Wheat Seedling ◽  
Morphological Parameters

scholarly journals Trabeculae microstructure parameters serve as effective predictors for marginal bone loss of dental implant in the mandible

2020 ◽  
Author(s):  
Hengguo Zhang ◽  
Jie Shan ◽  
Ping Zhang ◽  
Hongbing Jiang
Keyword(s):  
Machine Learning ◽  
Bone Loss ◽  
Trabecular Bone ◽  
Dental Implant ◽  
Early Stage ◽  
Superior Performance ◽  
Morphological Parameters ◽  
Marginal Bone Loss ◽  
Microstructure Parameters ◽  
Functional Loading

Abstract Background : To investigate the effectiveness and feasibility of machine learning models based on trabecular microstructure parameters for predicting the occurrence of marginal bone loss (MBL) of the submerged dental implant in mandible. Methods : Clinical variables and morphological parameters of trabecular bone were collected from 81 subjects with submerged implants in the mandible (41 cases of abnormal MBL and 40 as normal controls). We measured the peri-implant MBL level by a cone-beam computed tomography (CBCT) at the follow-up of 20.95±2.67 months after functional loading. The morphological parameters and possible factors associated with MBL were collected in a mean of 3.98±1.06 months at the early loading stage. All variables were analyzed using correlation and covariance matrices. Support vector machine (SVM), artificial neural network (ANN), logistic regression (LR) model and random forest (RF) were actualized to predict abnormal MBL. Results : At the early stage of functional loading, the abnormal MBL cases showed a significant increase of structure model index (SMI) and trabecular pattern factor (Tb.Pf) in peri-implant. Meanwhile, SMI and Tb.Pf simultaneously revealed a significantly high positive correlation with MBL. The LR model exhibited the best outcome in predicting MBL (AUC = 0.956), followed by SVM (AUC = 0.928), RF (AUC = 0.917), ANN (AUC = 0.900), SMI alone (AUC = 0.705) and Tb.Pf alone (AUC = 0.663). Compared with one single predictor, all algorithm models yielded significantly superior performance. Conclusion : Abnormal MBL cases demonstrated the premonitory morphological variation in trabecular bone at the early stage. MBL prediction could be achieved by machine learning methods.

scholarly journals Classification of Sugarcane Genotypes Based on Heat Stress and Morphological Parameters

2013 ◽  
Vol 1 (2) ◽  
pp. 114 ◽  
Author(s):  
Syed Rizwan Abbas ◽  
Asad Hussain Shah ◽  
Aurangzeb Rao ◽  
Syed Dilnawaz Ahmad Gardazi ◽  
Syed Mubashir Sabir ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Heat Stress ◽  
Membrane Stability ◽  
Pigment Concentration ◽  
Moisture Loss ◽  
Morphological Parameters ◽  
Phenolic Contents ◽  
Relative Tolerance

Leaves of thirteen genotypes of sugarcane were treated in the oven for heat stress. The treated leaves were used for the estimation of phenolic contents and the measurement of lipid peroxidation. Minimum moisture loss is showed by CSSG-668 and maximum moisture loss showed by NSG-45. Maximum phenolic contents were observed in HSF-240 (65.7 mg GAE/100 ml) and minimum results showed by Lho 83-153 and CP-43-33 (32.98 and 33.78 mg GAE/100 ml). Lho 83-153, HSF-242 and S-2002-US-133 were showed high tolerant against heat therefor, showed higher membrane stability, maintenance of high fv/fm ratio under heat stress and lower lipid peroxidation of membranes. Hence, the relative tolerance of genotype to heat stress as reflected by its lower lipid peroxidation, higher membrane stability and pigment concentration is related to the levels of activity of its antioxidant enzymes. 

Trabeculae microstructure parameters serve as effective predictors for marginal bone loss of dental implant in the mandible 

2020 ◽  
Author(s):  
Hengguo Zhang ◽  
Jie Shan ◽  
Ping Zhang ◽  
Hongbing Jiang
Keyword(s):  
Machine Learning ◽  
Bone Loss ◽  
Trabecular Bone ◽  
Dental Implant ◽  
Early Stage ◽  
Superior Performance ◽  
Morphological Parameters ◽  
Marginal Bone Loss ◽  
Microstructure Parameters ◽  
Functional Loading

Abstract Background: To investigate the effectiveness and feasibility of machine learning models based on trabecular microstructure parameters for predicting the occurrence of marginal bone loss (MBL) of the submerged dental implant in mandible. Methods: Clinical variables and morphological parameters of trabecular bone were collected from 81 subjects with submerged implants in the mandible (41 cases of abnormal MBL and 40 as normal controls). We measured the peri-implant MBL level by a cone-beam computed tomography (CBCT) at the follow-up of 20.95±2.67 months after functional loading. The morphological parameters and possible factors associated with MBL were collected in a mean of 3.98±1.06 months at the early loading stage. All variables were analyzed using correlation and covariance matrices. Support vector machine (SVM), artificial neural network (ANN), logistic regression (LR) model and random forest (RF) were actualized to predict abnormal MBL.Results: At the early stage of functional loading, the abnormal MBL cases showed a significant increase of structure model index (SMI) and trabecular pattern factor (Tb.Pf) in peri-implant. Meanwhile, SMI and Tb.Pf simultaneously revealed a significantly high positive correlation with MBL. The SVM model exhibited the best outcome in predicting MBL (AUC = 0.967), followed by ANN (AUC = 0.928), LR (AUC = 0.906), RF (AUC = 0.842), SMI alone (AUC = 0.705) and Tb.Pf alone (AUC = 0.663). Compared with one single predictor, all algorithm models yielded significantly superior performance.Conclusion: Abnormal MBL cases demonstrated the premonitory morphological variation in trabecular bone at the early stage. MBL prediction could be achieved by machine learning methods.

scholarly journals PAR1-mediated c-Jun activation promotes heat stress-induced early stage apoptosis of human umbilical vein endothelial cells

2017 ◽  
Vol 15 (5) ◽  
pp. 2595-2603 ◽  
Author(s):  
Shuang Zhang ◽  
Yanan Liu ◽  
Zhenglian Wang ◽  
Jingxian Liu ◽  
Zhengtao Gu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Heat Stress ◽  
Early Stage ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals Effect Of Physical And Chemical Mutagens On Morphological Behavior Of Tomato (Solanum Lycopersicum) CV. “Rio Grande” Under Heat Stress Conditions.

2014 ◽  
Vol 70 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Naheed Akhtar
Keyword(s):  
Heat Stress ◽  
Gamma Radiation ◽  
Block Design ◽  
Research Work ◽  
Rio Grande ◽  
Stress Conditions ◽  
Morphological Parameters ◽  
Tomato Production ◽  
Chemical Mutagens ◽  
Physical And Chemical

Abstract Given the importance to tomato production under heat stress conditions in hot climates of Pakistan, the objective of this research work was to study the influence of temperature and application of physical and chemical mutagens on tomato (Lycopersicon esculentum L.,) CV. “Rio grande” Seeds were treated with chemical mutagen (Ethyl Methane Sulphonate) and physical mutagen (Gamma radiation). Plants were grown in open field conditions under day/night temperatures regimes at the experimental area of Department of Horticulture, faculty of crop and food sciences, PMAS-AAUR. The experiment was set twenty treatments and four replications in Complete Randomize Block Design. It is observed that by increasing dose of mutagens shows lethal effect and morphological parameters reduced significantly. But lower doses of EMS and Gamma radiation improve thermotolerance capacity significantly. Plants were investigated for different morphological parameters i.e Plant height, no. of leaves, leaf area, relative water contents of leaves, chlorophyll contents, cell viability (TCC Assay) and pollen germination. Among gamma rays 5Kr were the only dose which survived and bear fruits. Among EMS 4mM, 8 mM, 16mM performed better under heat stress conditions than other treatments.

154 Exposure of bulls to heat stress had deleterious effects on embryo development

2019 ◽  
Vol 31 (1) ◽  
pp. 202
Author(s):  
N. Llamas Luceño ◽  
M. Van Poucke ◽  
M. Batlle Perez ◽  
K. J. Szymanska ◽  
D. Angrimani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Embryo Development ◽  
Sperm Morphology ◽  
Early Stage ◽  
Sperm Quality ◽  
Advanced Stage ◽  
Cell Ratio ◽  
Frozen Semen ◽  
Significant Difference

Concerns about global climate change reducing animal fertility are arising. Therefore, our objective was to determine the effects of increased environmental temperature on Holstein bulls and its effects on sperm quality and embryo development. Frozen semen samples were obtained from 6 bulls exposed to natural heat stress (HS) in August 2016, compared with a lower temperature (control) in March 2016 (temperature-humidity index of up to 74.5 and 40.6, respectively). We evaluated sperm morphology, embryo development, gene expression, inner cell mass/trophectoderm ratio (ICM/TE), and apoptosis cell ratio of Day-8 blastocysts. Sperm morphology was evaluated using eosin/nigrosin staining. Blastocysts were produced by routine in vitro methods (Wydooghe et al. 2014 Reprod. Fertil. Dev.). Cleavage rates were determined 48h after insemination, and blastocyst rates were determined on Days 7 and 8. Expression of NANOG, SOX2, POU5F1, DNMT (1, 3a, 3b), HSP (A1a, A2, A8, 10, 60, 90), HSF1, IFNT2, H19, SNRPN, IGF2, IGF2R, MEST, PHLDA2, MEG3, MEG9, PEG10 and PLAGL1 were analysed. Total RNA was extracted from Day-8 blastocysts for gene expression analysis using RNeasy Micro Kit (Qiagen, Valencia, CA, USA). Reverse transcription and qPCR were performed with iScript (BioRad, Hercules, CA, USA) and SsoAdvanced™ Universal SYBR® Green Supermix (BioRad) in a CFX Connect system (BioRad). Blastocysts were differentially stained (Wydooghe et al. 2011 Anal. Biochem.) and analysed using a Leica TCS-SP8×confocal microscope (Leica Microsystems, Wetzlar, Germany). Data analyses included a GLM procedure and paired-samples Student’s t test (P ≤ 0.05). A normal distribution was verified with a UNIVARIATE procedure and Shapiro-Wilk test. A Wilcoxon signed rank test was used to analyse qPCR data. Detached heads (P=0.006) and coiled tails (P=0.018) were significantly lower in the HS group (4.9 and 0.2%, respectively) compared with the control (5.5 and 0.5%). Moreover, proximal droplets (P=0.051) were lower in the HS group (0.7%) compared with the control (1.3%). Remarkably, cleavage and blastocyst rates at Days 7 and 8 were significantly higher in control (78.4, 19.6 and 29.5%, respectively) compared with HS (75, 14.5 and 23.2%). Early and normal blastocysts were grouped as early stage, whereas expanded, hatching and hatched blastocysts were grouped as advanced stage. There was a significant reduction in the HS group of early stage blastocysts on Day 7 and of advanced stage blastocysts on Days 7 and 8. However, in Day-8 blastocysts, there was no significant difference in gene expression for any target gene. Moreover, there were no significant differences in total number of cells or apoptosis cell ratio in blastocysts. However, the ICM/TE ratio was significantly higher (P=0.021) in control (0.7) compared with HS (0.56). Sperm samples collected in August had reduced fertility compared with those obtained in March. Although fewer sperm abnormalities were present in HS, based on decreased blastocyst rates and ICM/TE ratio in embryos produced with HS semen, we inferred that molecular mechanisms for advanced blastocyst development were affected. However, those mechanisms did not involve our target genes. This work was funded by the European Union, Horizon 2020 Marie Sklodowska-Curie Action, REPBIOTECH 675526.

scholarly journals In VitroAnalyses of Mild Heat Stress in Combination with Antifungal Agents against Aspergillus fumigatus Biofilm

2013 ◽  
Vol 58 (3) ◽  
pp. 1443-1450 ◽  
Author(s):  
Rong Zeng ◽  
Min Li ◽  
Qing Chen ◽  
Le Wang ◽  
Ping Zhan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Aspergillus Fumigatus ◽  
Late Stage ◽  
Biofilm Formation ◽  
Early Stage ◽  
Antifungal Drugs ◽  
Laser Scanning Microscopy ◽  
Content Type ◽  
Biomass Formation ◽  
Mild Heat

ABSTRACTAspergillus fumigatusbiofilms still present a challenge for effective treatment in clinical settings. While mild heat stress has been introduced as a treatment for infectious diseases, the effectiveness of mild heat stress onA. fumigatusbiofilm formation and antifungal susceptibility is still unknown. In the present study, confocal laser scanning microscopy (CLSM) was used to image and quantifyAspergillus fumigatusbiofilm formation under three different regimens of continuous mild heat stress: at 37, 39, and 41°C. Furthermore, fungal growth has been investigated under the above conditions in combination with antifungal drugs (amphotericin B [AMB], micafungin [MCF], and voriconazole [VOC]) at early and late stages. CLSM analysis showed that higher temperatures induce earlier germination and greater hyphal elongation but poorer polar growth and reduced biofilm thickness. In the early stage of biofilm formation, the combination of treatment at 39 or 41°C with MCF or VOC produced no visible difference in biomass formation from similar treatments at 37°C with the same drug. Interestingly, AMB treatment at 37°C inhibited early stage biofilm formation to a much greater extent than at 39 and 41°C. At the late stage of biofilm formation, the mild heat treatments at 39 and 41°C with AMB, MCF, and VOC inhibited biomass formation compared to that at 37°C. The present data show that mild heat stress has a negative regulatory effect on biofilm formationin vitro, and antifungal drug improvement with mild heat treatment at late-stage biofilm formation provides useful indications of possible effective strategies for clinical management of aspergillosis.

scholarly journals Factors affecting the rapid changes of protein under short-term heat stress

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Bingjin Wu ◽  
Jianwen Qiao ◽  
Xiaoming Wang ◽  
Manshuang Liu ◽  
Shengbao Xu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Protein Expression ◽  
Protein Content ◽  
Early Stage ◽  
Protein Translation ◽  
Short Term ◽  
Factors Affecting ◽  
Thermal Environments ◽  
Rapid Changes ◽  
The Impact

Abstract Background Protein content determines the state of cells. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. Results We quantified 47,535 mRNAs and 3742 proteins in the filling grains of wheat in two different thermal environments. The impact of mRNA abundance and sequence features involved in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency were the main drivers of changes in protein expression under heat stress, and their combined contribution explains 58.2 and 66.4% of the protein variation at 30 and 40 °C (20 °C as control), respectively. Transcription contributes more to alterations in protein content at 40 °C (31%) than at 30 °C (6%). Furthermore, the usage of codon AAG may be closely related to the rapid alteration of proteins under heat stress. The contributions of AAG were 24 and 13% at 30 and 40 °C, respectively. Conclusion In this study, we analyzed the factors affecting the changes in protein expression in the early stage of heat stress and evaluated their influence.

scholarly journals Application of Reflectance Spectroscopy in `Fuji' Apple Sunburn Early Detection

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 772B-772
Author(s):  
Pinghai Ding* ◽  
Leslie H. Fuchigami
Keyword(s):  
Heat Stress ◽  
Early Detection ◽  
Early Stage ◽  
Reflectance Spectroscopy ◽  
The Sun ◽  
Symptom Development ◽  
Natural Conditions ◽  
Controlled Conditions ◽  
Fuji Apple ◽  
Temperature Controlled

Sunburn can be caused either by heat stress or by UV-B radiation. Under natural conditions fruit sunburn is usually caused by the combination of both heat stress and UV-B radiation. To evaluate the use of reflectance spectroscopy in early detection of fruit sunburn, 5-year-old `Fuji' apple bearing trees growing in pot-in-pot system at the Lewis-Brown Horticulture Farm of Oregon State Univ. were used in the experiment. Fruit sunburn was monitored either under natural conditions or treated with UV-B, heat or both under controlled conditions after detached from the tree. Under natural conditions, the sun-exposed side of Fuji fruit has much higher anthocyanins than the shaded side. The increase in anthocyanins at the sun-exposed side is to protect the fruit from sunburn. When the temperature higher than 40 °C the sunburn symptom initiated first by change the color of the sun-exposed side from red to tan, then to yellow patches, and finally turn to brown and dark brown patches. The peel pigments analysis results indicated that anthocyanins decreased earlier than chlorophyll in the symptom development. The results of detached fruit exposure to 30,000μW/cm2 UV-B lights or to 40 °C at control conditions for 2, 4, 6, 8, 10, and 12 hours indicated that high intensity UV-B lights or high temperature alone can cause sunburn respectively. The UV-B and temperature combination treatment enhanced the sunburn processes. The apples with sunburn caused under natural conditions or under UV-B and temperature controlled conditions were scanned by using reflectance spectroscopy of FOSS NIR system at different stage of the sunburn. The results indicted that Fuji apple sunburn can be efficiently detected at the early stage of sunburn in both natural and controlled conditions.

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