Application of Ultrasound-Assisted Extraction on the Stem Bark of Rhinachantus Nasutus (L.) Kurz, Total Phenolic, and Its Potential as Antioxidant and Inhibitor of Alpha-Glucosidase Enzyme Activity

Valorization of an artichoke by-product, rich in bioactive compounds, by ultrasound-assisted extraction, is proposed. The extraction yield curves of total phenolic content (TPC) and chlorogenic acid content (CAC) in 20% ethanol (v/v) with agitation (100 rpm) and ultrasound (200 and 335 W/L) were determined at 25, 40, and 60 °C. A mathematical model considering simultaneous diffusion and convection is proposed to simulate the extraction curves and to quantify both temperature and ultrasound power density effects in terms of the model parameters variation. The effective diffusion coefficient exhibited temperature dependence (72% increase for TPC from 25 °C to 60 °C), whereas the external mass transfer coefficient and the equilibrium extraction yield depended on both temperature (72% and 90% increases for TPC from 25 to 60 °C) and ultrasound power density (26 and 51% increases for TPC from 0 (agitation) to 335 W/L). The model allowed the accurate curves simulation, the average mean relative error being 5.3 ± 2.6%. Thus, the need of considering two resistances in series to satisfactorily simulate the extraction yield curves could be related to the diffusion of the bioactive compound from inside the vegetable cells toward the intercellular volume and from there, to the liquid phase.

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Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

International Journal of Food Engineering ◽

10.1515/ijfe-2019-0191 ◽

2020 ◽

Vol 16 (1-2) ◽

Cited By ~ 2

Author(s):

Cassiano Brown da Rocha ◽

Caciano Pelayo Zapata Noreña

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Grape Pomace ◽

Total Phenolic ◽

Microwave Assisted Extraction ◽

Ultrasound Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

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Agro-industrial waste as a source of bioactive compounds: ultrasound-assisted extraction from blueberry (Vaccinium myrtillus) and raspberry (Rubus idaeus) pomace

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v43i1.55567 ◽

2021 ◽

Vol 43 ◽

pp. e55564

Author(s):

Suelen Siqueira dos Santos ◽

Carolina Moser Paraíso ◽

Letícia Misturini Rodrigues ◽

Grasiele Scaramal Madrona

Keyword(s):

Bioactive Compounds ◽

Energy Efficient ◽

Extraction Method ◽

Rubus Idaeus ◽

Total Phenolic ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Conventional Extraction ◽

Gallic Acid Equivalent

Blueberry and raspberry pomace are a rich source of bioactive compounds that have not been commercially utilized yet, and ultrasound-assisted technology can efficiently extract these compounds. Also, the use of water as a solvent added to the ultrasound-assisted technology improves this eco-friendly process. Therefore, an aqueous eco-friendly extraction, including extraction time and ultrasound presence or absence (conventional extraction) was performed in order to extract bioactive compounds from blueberry and raspberry pomace. Response parameters included levels of anthocyanins, phenolic compounds, and flavonoids, and antioxidant activity determined by DPPH, ABTS, and FRAP methods. Analysis of variance results indicated that ultrasound-assisted extraction for 45 min. was feasible to extract the bioactive compounds. The antioxidant content of the extract obtained by the ultrasound-assisted process was 1.4 times higher on average and the total phenolic concentration was 1.6 times higher (for blueberry 5.02 and for raspberry 2.53 mg gallic acid equivalent/g) compared with those obtained by the conventional process. Thus, the ultrasound-assisted extraction method can be a profitable alternative to extract bioactive compounds from blueberry and raspberry pomace, as it is energy efficient, requires fewer chemicals, and produces less effluent. This eco-friendly technology is therefore viable for food, nutraceutical, and cosmetic industries, and also for reducing food waste.

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Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe oleracea Mart.) Using an Experimental Design Methodology. Part 2: Ultrasound-Assisted Extraction

Agronomy ◽

10.3390/agronomy10030326 ◽

2020 ◽

Vol 10 (3) ◽

pp. 326 ◽

Cited By ~ 4

Author(s):

María José Aliaño-González ◽

Estrella Espada-Bellido ◽

Marta Ferreiro-González ◽

Ceferino Carrera ◽

Miguel Palma ◽

...

Keyword(s):

Phenolic Compounds ◽

Bioactive Compounds ◽

Total Phenolic ◽

Solvent Ratio ◽

Total Phenolic Compounds ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Relative Standard ◽

Ultrasound Assisted ◽

Optimized Conditions

Two optimized methods for ultrasound-assisted extraction were evaluated for the extraction of two types of acai bioactive compounds: Total anthocyanins (TAs) and total phenolic compounds (TPCs). For the extraction optimization, a Box Behnken factorial design of different variables in the following intervals was used: Methanol-water (25%–75%) for solvent composition, temperatures between 10 and 70 °C, amplitude in the range between 30% and 70% of the maximum amplitude −200 W), extraction solvent pH (2–7), the ratio for sample-solvent (0.5 g:10 mL–0.5 g:20 mL), and cycle between 0.2 and 0.7 s. The extraction kinetics were studied using different periods between 5 and 30 min. TA and TPC were analyzed by UHPLC and the Folin–Ciocalteu method, respectively. Optimized conditions for TA were: 51% MeOH in water, 31 °C temperature, pH 6.38, cycle 0.7 s, 65% amplitude, and 0.5 g:10 mL of sample-solvent ratio. Optimized conditions for the TPC were: 49% MeOH in water, 41 °C temperature, pH 6.98, cycle 0.2 s, 30% amplitude, and 0.5 g:10 mL of sample-solvent ratio. Both methods presented a relative standard deviation below 5% in the precision study. The suitability of the methods was tested in real samples. It was confirmed that these methods are feasible for the extraction of the studied bioactive compounds from different açai matrices.

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Optimization of Ultrasound Assisted Extraction of Functional Ingredients from Stevia Rebaudiana Bertoni Leaves

International Agrophysics ◽

10.1515/intag-2015-0017 ◽

2015 ◽

Vol 29 (2) ◽

pp. 231-237 ◽

Cited By ~ 38

Author(s):

Jana Šic Žlabur ◽

Sandra Voća ◽

Nadica Dobričević ◽

Mladen Brnčić ◽

Filip Dujmić ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Extraction Procedure ◽

Total Phenolic ◽

Steviol Glycosides ◽

Total Phenolic Compounds ◽

Ultrasound Assisted Extraction ◽

Probe Diameter ◽

Assisted Extraction ◽

Ultrasound Assisted

Abstract The aim of the present study was to reveal an effective extraction procedure for maximization of the yield of steviol glycosides and total phenolic compounds as well as antioxidant activity in stevia extracts. Ultrasound assisted extraction was compared with conventional solvent extraction. The examined solvents were water (100°C/24 h) and 70% ethanol (at 70°C for 30 min). Qualitative and quantitative analyses of steviol glycosides in the extracts obtained were performed using high performance liquid chromatography. Total phenolic compounds, flavonoids, and radical scavenging capacity by 2, 2-azino-di-3-ethylbenzothialozine- sulphonic acid) assay were also determined. The highest content of steviol glycosides, total phenolic compounds, and flavonoids in stevia extracts were obtained when ultrasound assisted extraction was used. The antioxidant activity of the extracts was correlated with the total amount of phenolic compounds. The results indicated that the examined sonication parameters represented as the probe diameter (7 and 22 mm) and treatment time (2, 4, 6, 8, and 10 min) significantly contributed to the yield of steviol glycosides, total phenolic compounds, and flavonoids. The optimum conditions for the maximum yield of steviol glycosides, total phenolic compounds, and flavonoids were as follows: extraction time 10 min, probe diameter 22 mm, and temperature 81.2°C.

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Evaluation of Direct Ultrasound-Assisted Extraction of Phenolic Compounds from Potato Peels

Processes ◽

10.3390/pr8121665 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1665

Author(s):

Shusheng Wang ◽

Amy Hui-Mei Lin ◽

Qingyou Han ◽

Qin Xu

Keyword(s):

Phenolic Compounds ◽

Agricultural Waste ◽

The United States ◽

Total Phenolic ◽

Systematic Research ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Potato Peels ◽

Ultrasound Assisted ◽

The Impact

Potato peels (PPs) are generally considered as agriculture waste. The United States alone generates over one million tons of PPs a year. However, PPs contain valuable phenolic compounds with antioxidant activities. In this study, we evaluated the efficiency of ultrasound-assisted extraction techniques in recovering antioxidants from PPs. These techniques included a direct ultrasound-assisted extraction (DUAE), an indirect ultrasound-assisted extraction (IUAE), and a conventional shaking extraction (CSE). Results of this study showed that DUAE was more effective in extracting phenolic compounds than IUAE and CSE. We also evaluated the factors affecting the yield of total phenolic compounds (TPC) in DUAE, including the temperature, time, acoustic power, ratio of solvent to solids, and size of PPs particles. TPC yield of DUAE was higher, and the extraction rate was faster than IUAE and CSE. Furthermore, TPC yield was strongly correlated to the temperature of the mixture of PPs suspension. SEM images revealed that the irradiation of ultrasound energy from DUAE caused micro-fractures and the opening of PPs cells. The extract obtained from DUAE was found to have antioxidant activity comparable to commercial synthetic antioxidants. Results of this preliminary study suggest that DUAE has the potential to transform PPs from agricultural waste to a valuable ingredient. A future systematic research study is proposed to advance the knowledge of the impact of processing parameters in the kinetics of phenolic compounds extraction from potato peels using various extraction methods.

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Optimization of Ultrasound-Assisted Extraction of Polyphenols from Myrtus communis L. Pericarp

Antioxidants ◽

10.3390/antiox8070205 ◽

2019 ◽

Vol 8 (7) ◽

pp. 205 ◽

Cited By ~ 7

Author(s):

Nadia Bouaoudia-Madi ◽

Lila Boulekbache-Makhlouf ◽

Khodir Madani ◽

Artur M.S. Silva ◽

Sofiane Dairi ◽

...

Keyword(s):

Gallic Acid ◽

Irradiation Time ◽

Dry Weight ◽

Total Phenolic ◽

Ionization Mass ◽

Myrtus Communis ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Gallic Acid Equivalent

Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of Myrtus communis using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MSn). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (p < 0.0001) and amplitude (p = 0.0421) and optimal parameters conditions set by the RSM model were 70% (v/v), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from M. communis pericarp, enabling to reduce the working time and the solvent consumption.

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Optimization of Total Phenol and Tannin Content and Biological Activity of Dittrichia graveolens (L.) GREUTER.

Current Bioactive Compounds ◽

10.2174/1573407214666180730110830 ◽

2020 ◽

Vol 16 (2) ◽

pp. 124-132 ◽

Cited By ~ 2

Author(s):

Mahsa Souri ◽

Alireza Shakeri

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Methanol Concentration ◽

Total Phenolic ◽

Tannin Content ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.

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Ultrasound-assisted extraction of bioactive compounds from lemon balm and peppermint leaves

International Agrophysics ◽

10.1515/intag-2015-0077 ◽

2016 ◽

Vol 30 (1) ◽

pp. 95-104 ◽

Cited By ~ 10

Author(s):

Jana Šic Žlabur ◽

Sandra Voća ◽

Nadica Dobričević ◽

Stjepan Pliestić ◽

Ante Galić ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Radical Scavenging ◽

Ultrasonic Extraction ◽

Total Phenolic ◽

Total Phenols ◽

Ultrasound Assisted Extraction ◽

Lemon Balm ◽

Assisted Extraction ◽

Ultrasound Assisted

Abstract The aim of this study was to investigate the influence of conventional and ultrasound-assisted extraction (frequency, time, temperature) on the content of bioactive compounds as well as on the antioxidant activity of aqueous extracts from fresh lemon balm and peppermint leaves. Total phenols, flavonoids, non-flavonoids, total chlorophylls, total carotenoids, and radical scavenging capacity were determined. Moreover, the relationship between bioactive compounds and antioxidant capacity was studied by linear regression. A significant increase in all studied bioactive compounds during ultrasonic extraction for 5 to 20 min was found. With the classical extraction method, the highest amounts of total phenols, flavonoids, and antioxidant activity were determined, and the maximum amounts of total chlorophylls and carotenoids were determined during 20 min ultrasonic extraction. The correlation analysis revealed a strong, positive relationship between antioxidant activity and total phenolic compounds.

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