The Studies on pesticide properties from indigenous medicinal plants; antibacterial activity on plant pathogenic bacteria, Pseudomonas.syringae

2021 ◽  
Vol 31 (3) ◽  
pp. 120-126
Author(s):  
Undarmaa Davaasambuu ◽  
Narantsatsralt Davaadorj

We tested the extracts from plants growing in Mongolia, such as Chelidonium majus, Artemisia annua L, and Hyoscyamus niger L, against bacterial angular spot plant disease, which is the most common and harmful in the greenhouses of Mongolia to determine a bactericidal activity of these plants in vitro on plant pathogenic bacteria, Pseudomonas.syringae The extracts of selected plants were used at  0.3, 0.6, 1.2, 2.5, 5.0, 10.0,15.0  mg/ml concentrates against plant pathogenic P.syringae bacteria, among them 0.3, 0.6 mg/ml concentrates did not show the zones of growth inhibition (ZGI). The zones of growth inhibition were observed at 1.2 mg/ml; it  considered as  minimum inhibition concentrate (MIC).  The extract of Chelidonium majus, at MIC 1.2 mg/ml concentrate, the zones of growth inhibition  9±0.58mm, at 15 mg/ml concentrate 18.6±0.33mm; the extract of Artemisia annua L, at MIC 1.2 mg/ml concentrate, 7.3±0.33 mm, at 15 mg/ml concentrate 17.6±0.33mm, and the extract of Hyoscyamus niger L, at MIC 1.2 mg/ml concentrate,     7.3±0.33 mm, at 15 mg/ml concentrate 17.6±0.33mm were observed respectively.  Antibacterial activity of the extract of Chelidonium majus, 55.1-107.8%, the extract of Artemisia annua L 44.89-97.94%, and the extract of Hyoscyamus niger L 36.74-90.42%   against plant pathogenic bacteria, P.syringae be compared to a positive control (Kanamycin) estimated. Among these plant extracts, the highest antibacterial activity found at 5-15 mg/ml concentrates of the extract of Chelidonium majus, which was over than standard solution (Kanamycin). Ургамлын пестицид шинж чанарын судалгаа; фитопатоген Pseudomonas.syringae бактерийн эсрэг идэвхиийг тодорхойлсон дүн Монгол орны уугууль эмт шинж чанар бүхий тарималжсан их шүүдэргэнэ(Chelidonium majus), байгалийн морин шарилж (Artemisia annua L), хар лантанз (Hyoscyamus niger L) зэрэг ургамлаас өтгөн ханд бэлтгэж, фитопатоген Pseudomonas.syringae бактерийн эсрэг туршиж, пестицид (бактерицид) шинж чанарыг судлав. Ургамлуудын хандыг 0.3, 0.6, 1.2, 2.5, 5.0, 10.0,15.0  мг/мл концентрацитайгаар тус тус авч фитопатоген P.syringae бактерийн эсрэг туршихад, 1.2 мг/мл концентрациас эхлэн мэдэгдэхүйц зоныг үүсгэсэн нь энэхүү бактерийн эсрэг идэвхитэй хамгийн бага концентраци болохыг тодорхойлов. Их шүүдэргэнэ ургамлын ханд нь хамгийн бага идэвхтэй 1.2 мг/мл концентрацидаа 9±0.58 мм, хамгийн их 15 мг/мл-дээ 18.6±0.33мм, Морин шарилж ургамлын ханд нь хамгийн бага идэвхтэй 1.2 мг/мл концентрацидаа 7.3±0.33 мм, хамгийн их 15 мг/мл-дээ 17.6±0.33мм, харин Хар лантанз ургамлын ханд нь хамгийн бага идэвхтэй 1.2 мг/мл концентрацидаа 7.3±0.33 мм, хамгийн их 15 мг/мл-дээ 17.6±0.33мм зоныг тус тус үүсгэсэнээс үзэхэд эдгээр ургамлуудын ханд нь энэхүү фитопатоген бактерийг дарангуйлах өндөр идэвхитэй  байна. Их шүүдэргэнэ ургамлын ханд нь эерэг хяналттай харьцуулахад 55.1-107.8%, морин шарилжийн ханд 44.89-97.94% , хар лантанз ургамлын ханд нь 36.74-90.42% зэрэг бактерийн өнцгөн толбожилт өвчнийг үүсгэдэг P.syringae бактерийг эсрэг идэвхийг үзүүлснээс их шүүдэргэнэ ургамлын ханд нь дарангуйлах шинж чанарын хувьд эерэг хяналтаас давсан өндөр үр дүнг үзүүлэв.  Түлхүүр үг: ургамлын ханд,  пестицид (бактерицид) шинж чанар, in vitro, фитопатоген бактери

2011 ◽  
Vol 6 (34) ◽  
pp. 6829-6834, ◽  
Author(s):  
Tao Ke ◽  
Fan Jieyu ◽  
Shi Guanying ◽  
Zhang Xingang ◽  
Zhao Haoyu ◽  
...  

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Mohamed E. I. Badawy ◽  
Entsar I. Rabea ◽  
Nehad E. M. Taktak ◽  
Mahmoud A. M. El-Nouby

This study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kDa in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria,Agrobacterium tumefaciens,Erwinia carotovora,Corynebacterium fascians, andPseudomonas solanacearum. The antibacterial activity was evaluatedin vitroby the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested. The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria. The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.


2018 ◽  
Vol 78 (4) ◽  
pp. 691-696 ◽  
Author(s):  
G. L. S. Ferreira ◽  
L. M. D. Bezerra ◽  
I. L. A. Ribeiro ◽  
R. C. D. Morais Júnior ◽  
R. D. Castro

Abstract This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium. After incubation for 24 hours in an oven at 37 °C, plate reading was completed and confirmed by visual method using 2,3,5 triphenyl tetrazolium chloride dye. The Minimum Bactericidal Concentration (MBC) was determined from MIC subcultures. Assays were performed in triplicate, and chlorhexidine was used as a positive control. The diameters in mm of the growth inhibition halos ranged between 7.3 and 10.7 for S. mutans, 7.3 and 10.0 for S. oralis, and 8.2 and 9.8 for S. salivarius. The MIC and MBC values obtained converged, ranging from maximum values in the presence of Linalool (1,250.0 mg/mL, 2,500.0 mg/mL and 2,500.0 mg/mL, respectively, for S. mutans, S. oralis, and S. salivarius); and minimum values with Thymol (312.5 μg/ml, 156.2 μg/mL and 156.2 μg/ml, respectively for S. mutans, S. oralis, and S. salivarius). All the tested phytochemicals displayed antibacterial activity, thus representing substances with potential applications in preventing tooth decay.


2018 ◽  
Vol 33 (3-4) ◽  
pp. 185-195 ◽  
Author(s):  
Tatjana Popovic ◽  
Zoran Milicevic ◽  
Violeta Oro ◽  
Igor Kostic ◽  
Vesela Radovic ◽  
...  

Numerous scientific research studies all over the world have addressed the problem of agriculture in the 21st century as being particularly sensitive to climate change, which has caused phytopathogenic bacteria to spread. Therefore, there is a clear and urgent need to contain this kind of risk in agricultural production (both conventional and organic farming). The objective of this study was to determine the antibacterial activity of 30 essential oils (EOs) against three harmful plant pathogenic bacteria of agricultural importance, Erwinia amylovora, Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. syringae. The study included in vitro testing, using an agar-diffusion assay. The EOs of Ceylon cinnamon (leaf and bark), oregano, clove bud and palmarosa revealed antibacterial activity against the test bacteria, and the maximum mean inhibition zone diameters of 35 mm was found against E. amylovora and X. campestris pv. campestris (highly sensitive reaction), while it was smaller in the case of P. syringae pv. syringae, from 18.25-26.25 mm (sensitive to very sensitive reaction). Maximum diameter of the zone of inhibition (35 mm) was obtained using basil and peppermint against E. amylovora, and rosemary, blue gum and camphor tree against X. campestris pv. campestris. Not a single EO inhibited P. syringae pv. syringae with the resulting total diameter zone of 35 mm, and this test bacteria was resultingly classified as the least susceptible bacterium of the three tested. EOs of lemongrass, aniseed, ylang ylang, silver fir, lemon, dwarf mountain pine, bay laurel and scots pine caused sensitive reaction of the tested bacteria. Peppermint, black cumin, Indian frankincense, bergamot orange, common juniper, bitter orange and neem produced variable reactions from total to weakly or no inhibition at all. Weakly activity was found in niaouli and Atlas cedar. Eastern red cedar, patchouli, Indian sandalwood and ginger caused no reaction of any of the test bacteria. The results offer a basis for further work based on in vivo testing for the purpose of developing ?natural pesticides? for control of phytopathogenic bacteria, thus giving a significant contribution to reducing yield losses in agriculture and sustainable development.


2018 ◽  
Vol 17 (6) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Tomasz M. Staniek ◽  
Elżbieta Paduch-Cichal ◽  
Beata Dasiewicz ◽  
Agnieszka Gadomska-Gajadhur ◽  
...  

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.


Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.


2011 ◽  
Vol 6 (2) ◽  
pp. 173 ◽  
Author(s):  
Rosmiati Rosmiati ◽  
Habsah Mohamad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Najiah Musa ◽  
Aziz Ahmad ◽  
...  

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.


2019 ◽  
Vol 57 (3B) ◽  
pp. 9 ◽  
Author(s):  
Tien Cuong Nguyen ◽  
Thi Anh Phuong Chu ◽  
Hai Van Nguyen

ABSTRACT-QMFS2019Poultry production provides source of protein and contributes an important income for Vietnamese farmers. Among the poultry in Vietnam, ducks account for 27.3% of head of poultry and even 55.7% in Mekong Delta region. Along with the development of rearing ducks, bacterial, viral and fungal diseases occurring in the two last decades induced bad effect for poultry producer. Escherichia coli, Salmonella enterica, Streptococcal or Pasteurella act as major pathogenic bacteria in duck. The aims of this study were to investigate the antibacterial activity of garlic Allium sativum against E. coli, Staphylococcus aureus, Salmonella Typhimurium and to evaluate the effect of garlic on growth performance of duck from 1-28 old-days. The results indicated that fresh garlic and dried garlic powder showed inhibitory effect against pathogenic tested strains from 2% and 4% w/v, respectively. The inhibition zones and the minimal inhibitory concentration (MIC) values of garlic extract ranged from 11.3-28.3 mm and 0.02-0.2 g/ml, respectively. After 28 days of diet with garlic supplemented, D3 (2% of fresh garlic in water) showed significantly different in weight gain (WG), feed conversion ratio (FCR), protein efficiency ratio (PER), average daily weight (ADW); whereas, D2 (2% of garlic powder in basal diet) only possessed a difference significant in feed consumption (FC) compared to the D1 (control without garlic supplementation). The obtained results demonstrated the potential of garlic application in poultry production.


Sign in / Sign up

Export Citation Format

Share Document