Influence of long-term cold stress on enzymatic antioxidative defense system in chickpea (Cicer arietinum L.)

Abiotic stresses such as cold, heat, and drought are the main causes of universal crop losses. Plants have generated adaptive responses which prevent them from oxidative damage caused by environmental stresses. The present research aimed to evaluate the effect of cold stress on lipid peroxidation and antioxidant enzyme activity in the leaves of eight cultivars / advanced lines of chickpea (<em>Cicer arietinum</em> L.). Three-week-old plantlets were subjected to cold stress (0°C) for 24 or 48 hours. Selected antioxidant enzyme activity and oxidative status of chickpea plantlets under cold stress were determined. In most genotypes, catalase and ascorbate peroxidase activities were increased and guaiacol peroxidase activity decreased under stress conditions but the activity of superoxide dismutase remained almost constant. Based on its ranking, <em>Cicer arietinum</em> ‘Saral’, a newly released cold-resistant Iranian chickpea cultivar, had the strongest, and FLIP 05-77C had the weakest antioxidative defense system under low temperature stress.

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Coronatine alleviates cold stress by improving growth and modulating antioxidative defense system in rice (Oryza sativa L.) seedlings

Plant Growth Regulation ◽

10.1007/s10725-021-00775-9 ◽

2021 ◽

Author(s):

Tianyu Feng ◽

Yan Zhang ◽

Min He ◽

Mingcai Zhang ◽

Zhaohu Li ◽

...

Keyword(s):

Oryza Sativa ◽

Cold Stress ◽

Oryza Sativa L ◽

Antioxidative Defense ◽

Defense System ◽

Antioxidative Defense System

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Study of the effect of abiotic stress on the antioxidant enzyme activity of cereals under regulated environmental conditions

Acta Agronomica Hungarica ◽

10.1556/aagr.61.2013.3.1 ◽

2013 ◽

Vol 61 (3) ◽

pp. 175-183

Author(s):

B. Varga ◽

S. Bencze ◽

T. Janda ◽

O. Veisz

Keyword(s):

Enzyme Activity ◽

Drought Stress ◽

Winter Wheat ◽

Antioxidant Enzyme ◽

Agricultural Research ◽

Hungarian Academy ◽

Antioxidant Enzyme Activity ◽

Guaiacol Peroxidase ◽

Wheat Varieties ◽

Winter Wheat Varieties

The impacts of climate modification were examined in terms of changes in the stress tolerance of winter wheat varieties. The enzyme reactions of two winter wheat varieties to drought stress, simulated by water withholding in three different phenophases, were analysed in a phytotron experiment in the Centre for Agricultural Research, Hungarian Academy of Sciences. Plants were raised either at ambient CO2 level or at twice this concentration. The quantities of glutathione reductase (GR), glutathione-S-transferase (GST), catalase (CAT), guaiacol peroxidase (POD) and ascorbate peroxidase (APX) were determined from leaf samples collected at the end of the drought treatment.The results showed that antioxidant enzymes may help to counterbalance the reactive oxygen species induced by stress during various stages of the vegetation period. Although there were substantial differences in the changes induced in the activity of individual enzymes by modifications in environmental factors, this activity and its response to stress depended not only on these factors, but also on the developmental stage of the plant. Modifications in enzyme activity could indicate that enhanced CO2 concentration delayed the development of drought stress up to first node appearance, and stimulated antioxidant enzyme activity when drought occurred during ripening.

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Changes in the growth and antioxidant enzyme activity of seedlings originating from wheat seeds subjected to accelerated ageing test

Agricultural Science and Technology ◽

10.15547/ast.2020.03.034 ◽

2020 ◽

Vol 12 (3) ◽

pp. 216-220

Author(s):

R. Chipilski ◽

B. Kyosev ◽

R. Cholakova

Keyword(s):

Enzyme Activity ◽

Antioxidant Enzyme ◽

Chlorophyll Content ◽

Germination Rate ◽

Morphological Characteristics ◽

Dry Weight ◽

Antioxidant Enzyme Activity ◽

Accelerated Ageing ◽

Guaiacol Peroxidase ◽

Wheat Seeds

Abstract. The objective of this study was to investigate the reaction processes of seedlings originating from wheat seeds after they were subjected to accelerated ageing, which imitated low-temperature storage. Germination, vigor, morphological characteristics, relative chlorophyll content and antioxidant enzyme activity were measured in seedlings and young plants of wheat (Triticum aestivum L.) after processes of accelerated ageing of the seeds. The ageing procedures of the seeds were done according to the standard conditions (40±0.5°C and 100% air relative humidity) of the International Seed Testing Association for 72 and 120 hours of treatments of the Bulgarian varieties Geya-1 and Sadovo 772. For the control were used seeds, which were not subjected to the accelerated ageing conditions. The coleoptile of 5 days seedlings and second leaf of 10 and 15 days plants in seedling stage, following the seed accelerated ageing procedure, was measured in the laboratory and the greenhouse. The seed germination rate and vigor, fresh and dry weight of growing coleoptiles were inhibited after different periods of ageing exposure and well correlated with increased accumulation of total hydrogen peroxide, malondialdehyde content, guaiacol peroxidase activity and rate of cells membrane stability index. The negative changes of chlorophyll content index, fresh and dry weight and leaf area of the leaves of young plants descending from aged seeds sowed in pots were found. It was established that the modern variety Geya-1 was more tolerant to the applied ageing condition than the older Sadovo 772. In conclusion, we considered that accelerated ageing could be used as a model for estimation of seed deterioration in wheat after long-term storage.

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