scholarly journals The effect of methyl jasmonate and phenolic acids on growth of seedlings and accumulation of anthocyanins in common buckwheat (Fagopyrum esculentum Moench)

2012 ◽  
Vol 62 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Marcin Horbowicz ◽  
Halina Mioduszewska ◽  
Danuta Koczkodaj ◽  
Marian Saniewski
Keyword(s):  
Methyl Jasmonate ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Primary Root ◽  
Fagopyrum Esculentum ◽  
Coumaric Acid ◽  
Common Buckwheat ◽  
Simultaneous Treatment ◽  
Primary Roots ◽  
Growth Of Seedlings

The effect of methyl jasmonate (JA-Me) and phenolic acids: <i>trans</i>-cinnamic acid (<i>t</i>-CA), <i>p</i>-coumaric acid (<i>p</i>-CA), salicylic acid (SA) as well as naringenine (NAR) on growth of seedlings and accumulation of anthocyanins in common buckwheat (<i>Fagopyrum esculentum</i> Moench) were studied. JA-Me and phenolics were applied to growth medium of 4-days etiolated buckwheat seedlings before their exposition to day/night (16h/8h) conditions. The increase of primary roots and hypocotyls length were measured after 3 days of seedling growth in such conditions. At the end of experiment the total anthocyanins contents were measured as well. Methyl jasmonate (JA-Me) and trans-cinnamic acid (<i>t</i>-CA) inhibited growth of the primary root in young buckwheat seedlings, while naringenine (NAR) had a stimulatory influence, and <i>p</i>-coumaric acid had no effect at all. None of investigated phenolics or JA-Me had an effect on the growth of buckwheat hypocotyls, except the mixture of JA-Me and <i>p</i>-coumarcic acid. JA-Me significantly decreased the anthocyanins level in buckwheat hypocototyls, but not in cotyledons. <i>trans</i>-Cinnamic acid, <i>p</i>-coumaric acid and naringenine had no significant influence on the anthocyanin level in hypocotyls and cotyledons of buckwheat seedlings. Simultaneous treatment of buckwheat seedlings with JA-Me and <i>t</i>-CA or <i>p</i>-CA did not change the inhibition of anthocyanins accumulation in buckwheat hypocotyls by JA-Me. In the hypocotyls of buckwheat treated with a mixture of JA-Me and NAR, or SA, a synergistic reduction of anthocyanins was observed.

scholarly journals The effect of cis-jasmone, jasmonic acid and methyl jasmonate on accumulation of anthocyanins and proanthocyanidins in seedlings of common buckwheat (Fagopyrum esculentum Moench)

2011 ◽  
Vol 78 (4) ◽  
pp. 271-277 ◽  
Author(s):  
Marcin Horbowicz ◽  
Halina Mioduszewska ◽  
Danuta Koczkodaj ◽  
Marian Saniewski
Keyword(s):  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Primary Root ◽  
Solution Concentration ◽  
Inhibitory Effect ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Primary Roots ◽  
Strong Inhibitory Effect ◽  
Fagopyrum Esculentum Moench

Effects of various jasmonates (methyl jasmonate, jasmonic acid, cis-jasmone) on anthocyanins and procyanidins content of, as well as on growth of common buckwheat (<em>Fagopyrum esculentum</em> Moench) seedlings were studied. The studied jasmonates were applied as solutions or vapors on four days seedlings, and the seedlings were grown during the next four days in day/night conditions (16/8 h). Afterwards anthocyanins and proanthocyanidins content, as well as elongation of primary roots and hypocotyls were measured. When applied as solutions cis-jasmone (JAS) stimulated the anthocyanins accumulation, but when used as vapors had tendency to decrease its accumulation in buckwheat hypocotyls. Jasmonic acid (JA) solutions slightly stimulated or had no effect on biosynthesis of anthocyanins in buckwheat hypocotyls, but used as vapors caused a decline of anthocyanins in buckwheat hypocotyls. Methyl jasmonate (MJ) clearly inhibited biosynthesis of anthocyanins in hypocotyls of buckwheat seedlings. The studied jasmonates had no influence on anthocyanins level in cotyledons of buckwheat seedlings, except cis-jasmone, which at the lowest solution concentration slightly enhanced biosynthesis of the pigments. Treatment of buckwheat seedlings with solutions of all jasmonates (10<sup>-8</sup> M, 10<sup>-6</sup> M and 10<sup>-4</sup> M) had no influence on the growth of buckwheat hypocotyls. Contrary to that observation vapors of the growth regulators in concentrations 10<sup>-4</sup> M, had a strong inhibitory effect on the growth of hypocotyls of buckwheat seedlings. Solutions of JA and MJ, as well as vapors of JA, MJ and JAS strongly inhibited the primary root growth of buckwheat seedlings, while JAS applied as solution had no such influence. MJ and JA caused much higher stimulation of proanthocyanidin biosynthesis in buckwheat hypocotyls than JAS.

scholarly journals Comparison of the response of seedlings of common buckwheat (Fagopyrum esculentum Moench) to glyphosate applied to the shoot or to the root zone

2018 ◽  
Vol 71 (1) ◽  
Author(s):  
Marcin Horbowicz ◽  
Henryk Dębski ◽  
Magdalena Szwed ◽  
Danuta Koczkodaj ◽  
Józef Klocek
Keyword(s):  
Shoot Growth ◽  
Root Zone ◽  
Photosynthetic Pigment ◽  
Primary Root ◽  
Growth Conditions ◽  
Fagopyrum Esculentum ◽  
Anthocyanin Content ◽  
Common Buckwheat ◽  
Primary Roots ◽  
Root And Shoot Growth

We examined the response of common buckwheat (<em>Fagopyrum esculentum</em> Moench) seedlings, as a nontarget plant, to various doses of glyphosate applied to the root zone or to the shoots. Glyphosate was used at 0.1, 0.5, and 1.0 mM concentrations. The study was conducted on seedlings grown in hydroponic cultures under controlled growth conditions. Primary root and shoot growth, anthocyanin and photosynthetic pigment contents were measured to assess the effects of exposure to glyphosate. Glyphosate applied to shoots had a considerably higher impact on the growth of primary roots and shoots of seedlings. Low glyphosate concentrations produced an increase in anthocyanin content of hypocotyls, regardless of the mode of its application. Increasing the concentration of glyphosate applied to the root zone resulted in a gradual increase in anthocyanin content in cotyledons. Our overall results show that in hydroponically grown common buckwheat seedlings, glyphosate is less phytotoxic when applied to the root zone than when applied to the shoot. Low doses of glyphosate applied to the root zone stimulate root and shoot growth and increase the anthocyanin levels in cotyledons. The phytotoxicity of glyphosate was decreased in the absence of mineral nutrients in the root zone of buckwheat seedlings.

scholarly journals Concentrations of Phenolic Acids Are Differently Genetically Determined in Leaves, Flowers, and Grain of Common Buckwheat (Fagopyrum esculentum Moench)

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1142
Author(s):  
Alena Vollmannová ◽  
Janette Musilová ◽  
Judita Lidiková ◽  
Július Árvay ◽  
Marek Šnirc ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Sinapic Acid ◽  
Fagopyrum Esculentum ◽  
Blood Cholesterol ◽  
Common Buckwheat ◽  
High Concentration ◽  
Phenolic Substances ◽  
Fagopyrum Esculentum Moench

Common buckwheat (Fagopyrum esculentum Moench) is a valuable source of proteins, B vitamins, manganese, tryptophan, phytochemicals with an antioxidant effect, and the natural flavonoid rutin. Due to its composition, buckwheat supports the human immune system, regulates blood cholesterol, and is suitable for patients with diabetes or celiac disease. The study aimed to compare the allocation of selected phenolic acids (neochlorogenic acid, chlorogenic acid, trans-caffeic acid, trans-p-coumaric acid, trans-sinapic acid, trans-ferulic acid) and flavonoids (rutin, vitexin, quercetin, kaempferol) in the leaves, flowers, and grain of buckwheat cultivars of different origin. The content of individual phenolics was determined by the HPLC-DAD method. The results confirmed the determining role of cultivar on the relative content of chlorogenic acid, trans-caffeic acid, trans-sinapic acid, vitexin, and kaempferol in buckwheat plants. A significantly negative correlation among concentrations of phenolic acids in different common buckwheat plant parts shows that there are different mechanisms of genetic influences on the concentration of phenolic substances in common buckwheat flowers, leaves, and grain. These differences should be taken into account when breeding buckwheat for a high concentration of selected phenolic substances.

METABOLISM OF PHENYLPROPANOID COMPOUNDS IN SALVIA: II. BIOSYNTHESIS OF PHENOLIC CINNAMIC ACIDS

1959 ◽  
Vol 37 (1) ◽  
pp. 537-547 ◽  
Author(s):  
D. R. McCalla ◽  
A. C. Neish
Keyword(s):  
Caffeic Acid ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Shikimic Acid ◽  
Sinapic Acid ◽  
Coumaric Acid ◽  
Cinnamic Acids ◽  
Phenyllactic Acid ◽  
Salvia Splendens ◽  
Specific Activities

p-Coumaric, caffeic, ferulic, and sinapic acids were found to occur in Salvia splendens Sello in alkali-labile compounds of unknown constitution. A number of C14-labelled compounds were administered to leafy cuttings of salvia and these phenolic acids were isolated after a metabolic period of several hours and their specific activities measured. Cinnamic acid, dihydrocinnamic acid, L-phenylalanine, and (−)-phenyllactic acid were found to be good precursors of the phenolic acids. D-Phenylalanine, L-tyrosine, and (+)-phenyllactic acid were poor precursors. A kinetic study of the formation of the phenolic acids from L-phenylalanine-C14 gave data consistent with the view that p-coumaric acid → caffeic acid → ferulic acid → sinapic acid, and that these compounds can act as intermediates in lignification. Feeding of C14-labelled members of this series showed that salvia could convert any one to a more complex member of the series but not so readily to a simpler member. Caffeic acid-β-C14 was obtained from salvia after the feeding of L-phenylalanine-β-C14 or cinnamic acid-β-C14, and caffeic acid labelled only in the ring was obtained after feeding generally labelled shikimic acid.

Biosynthesis of phenolic acids in tomato plants infected with Agrobacterium tumefaciens

1974 ◽  
Vol 52 (9) ◽  
pp. 2041-2047 ◽  
Author(s):  
Kailash C. Chadha ◽  
Stewart A. Brown
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Benzoic Acid ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Acid Metabolism ◽  
Oxidation Products ◽  
Causative Organism ◽  
Coumaric Acid ◽  
Tomato Plants ◽  
Qualitative And Quantitative

Tomato plants 21 days old were inoculated with Agrobacterium tumefaciens, the causative organism of crown gall, and 72 h later [3-14C]cinnamic or [carboxyl-14C]benzoic acid was administered by wick-feeding to these and to uninfected control plants. After a further 48 h both groups were examined for incorporation of label into phenolic acids. The pattern of incorporation into bound phenolic acids of the controls was in accord with biosynthetic pathways previously demonstrated in normal plants, but qualitative and quantitative divergence from this pattern was observed in the bound phenolic acids of the infected plants. The data suggest interference by the pathogen with β-oxidation of at least two phenylpropanoid acids of the lignification pathway. In infected plants, lowered incorporation of 14C from cinnamic acid into phenylpropanoid acids of the lignification pathway and their β-oxidation products was accompanied by the appearance of labelled o-coumaric acid, consistent with some diversion of cinnamic acid metabolism toward ortho-hydroxylation. Incorporation of 14C from benzoic acid into gentisic and especially salicylic acid of infected plants was much lower than in the controls.

The effect of methyl jasmonate on accumulation of 2-phenylethylamine and putrescine in seedlings of common buckwheat (Fagopyrum esculentum)

2010 ◽  
Vol 33 (3) ◽  
pp. 897-903 ◽  
Author(s):  
Marcin Horbowicz ◽  
Ryszard Kosson ◽  
Wiesław Wiczkowski ◽  
Danuta Koczkodaj ◽  
Joanna Mitrus
Keyword(s):  
Methyl Jasmonate ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat

METABOLISM OF PHENYLPROPANOID COMPOUNDS IN SALVIA: II. BIOSYNTHESIS OF PHENOLIC CINNAMIC ACIDS

1959 ◽  
Vol 37 (4) ◽  
pp. 537-547 ◽  
Author(s):  
D. R. McCalla ◽  
A. C. Neish
Keyword(s):  
Caffeic Acid ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Shikimic Acid ◽  
Sinapic Acid ◽  
Coumaric Acid ◽  
Cinnamic Acids ◽  
Phenyllactic Acid ◽  
Salvia Splendens ◽  
Specific Activities

p-Coumaric, caffeic, ferulic, and sinapic acids were found to occur in Salvia splendens Sello in alkali-labile compounds of unknown constitution. A number of C14-labelled compounds were administered to leafy cuttings of salvia and these phenolic acids were isolated after a metabolic period of several hours and their specific activities measured. Cinnamic acid, dihydrocinnamic acid, L-phenylalanine, and (−)-phenyllactic acid were found to be good precursors of the phenolic acids. D-Phenylalanine, L-tyrosine, and (+)-phenyllactic acid were poor precursors. A kinetic study of the formation of the phenolic acids from L-phenylalanine-C14 gave data consistent with the view that p-coumaric acid → caffeic acid → ferulic acid → sinapic acid, and that these compounds can act as intermediates in lignification. Feeding of C14-labelled members of this series showed that salvia could convert any one to a more complex member of the series but not so readily to a simpler member. Caffeic acid-β-C14 was obtained from salvia after the feeding of L-phenylalanine-β-C14 or cinnamic acid-β-C14, and caffeic acid labelled only in the ring was obtained after feeding generally labelled shikimic acid.

scholarly journals Effects of Common Forage Phenolic Acids on Escherichia coli O157:H7 Viability in Bovine Feces

2005 ◽  
Vol 71 (12) ◽  
pp. 7974-7979 ◽  
Author(s):  
J. E. Wells ◽  
E. D. Berry ◽  
V. H. Varel
Keyword(s):  
Escherichia Coli ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Corn Silage ◽  
Escherichia Coli O157 ◽  
Coumaric Acid ◽  
E Coli ◽  
Forage Plants ◽  
Ruminant Animals ◽  
Bovine Feces

ABSTRACT Ruminant animals are carriers of Escherichia coli O157:H7, and the transmission of E. coli O157:H7 from cattle to the environment and to humans is a concern. It is unclear if diet can influence the survivability of E. coli O157:H7 in the gastrointestinal system or in feces in the environment. Feces from cattle fed bromegrass hay or corn silage diets were inoculated with E. coli O157:H7, and the survival of this pathogen was analyzed. When animals consumed bromegrass hay for <1 month, viable E. coli O157:H7 was not recovered after 28 days postinoculation, but when animals consumed the diet for >1 month, E. coli O157:H7 cells were recovered for >120 days. Viable E. coli O157:H7 cells in feces from animals fed corn silage were detected until day 45 and differed little with the time on the diet. To determine if forage phenolic acids affected the viability of E. coli O157:H7, feces from animals fed corn silage or cracked corn were amended with common forage phenolic acids. When 0.5% trans-cinnamic acid or 0.5% para-coumaric acid was added to feces from silage-fed animals, the E. coli O157:H7 death rate was increased significantly (17-fold and 23-fold, respectively) compared to that with no addition. In feces from animals fed cracked corn, E. coli O157:H7 death rates were increased significantly with the addition of 0.1% and 0.5% trans-cinnamic acid (7- and 13-fold), 0.1% and 0.5% p-coumaric acid (3- and 8-fold), and 0.5% ferulic acid (3-fold). These data suggest that phenolic acids common to forage plants can decrease viable counts of E. coli O157:H7 shed in feces.

scholarly journals Effect of Methyl Jasmonate on the Terpene Trilactones, Flavonoids, and Phenolic Acids in Ginkgo biloba L. Leaves: Relevance to Leaf Senescence

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4682
Author(s):  
Marcin Horbowicz ◽  
Wiesław Wiczkowski ◽  
Justyna Góraj-Koniarska ◽  
Kensuke Miyamoto ◽  
Junichi Ueda ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Phenolic Acids ◽  
Leaf Senescence ◽  
Ginkgo Biloba ◽  
Basic Research ◽  
Application Site ◽  
Coumaric Acid ◽  
Terpene Trilactones ◽  
The Difference ◽  
Acid Esters

The present study compared the effects of natural senescence and methyl jasmonate (JA-Me) treatment on the levels of terpene trilactones (TTLs; ginkgolides and bilobalide), phenolic acids, and flavonoids in the primary organs of Ginkgo biloba leaves, leaf blades, and petioles. Levels of the major TTLs, ginkgolides B and C, were significantly higher in the leaf blades of naturally senesced yellow leaves harvested on 20 October compared with green leaves harvested on 9 September. In petioles, a similar effect was found, although the levels of these compounds were almost half as high. These facts indicate the importance of the senescence process on TTL accumulation. Some flavonoids and phenolic acids also showed changes in content related to maturation or senescence. Generally, the application of JA-Me slightly but substantially increased the levels of TTLs in leaf blades irrespective of the difference in its application side on the leaves. Of the flavonoids analyzed, levels of quercetin, rutin, quercetin-4-glucoside, apigenin, and luteolin were dependent on the JA-Me application site, whereas levels of (+) catechin and (−) epicatechin were not. Application of JA-Me increased ferulic acid and p-coumaric acid esters in the petiole but decreased the levels of these compounds in the leaf blade. The content of p-coumaric acid glycosides and caffeic acid esters was only slightly modified by JA-Me. In general, JA-Me application affected leaf senescence by modifying the accumulation of ginkogolides, flavonoids, and phenolic acids. These effects were also found to be different in leaf blades and petioles. Based on JA-Me- and aging-related metabolic changes in endogenous levels of the secondary metabolites in G. biloba leaves, we discussed the results of study in the context of basic research and possible practical application.

Sign in / Sign up

Export Citation Format

Share Document