Verification of the systematic position of California brome (Bromus carinatus Hook. and Arn., Poaceae), cv. 'Broma', on the basis of analysis of issr markers

‘Broma’ is a grass cultivar belonging to the species Bromus carinatus. In the Lists of Agricultural Plant Varieties of the Research Centre for Cultivar Testing (COBORU), it is shown as Bromus willdenowii (= B. catharticus, B. unioloides) (List of Agricultural Plant Varieties 1989-2009), whereas already in 1984 Mirek demonstrated on the basis of morphological analysis that this was a different closely related species – B. carinatus. The aim of the present study was to verify the species affiliation of cv. ‘Broma’. The conducted analysis of ISSR molecular markers included representatives of cv. ‘Broma” as well as of B. carinatus and B. willdenowii. The method used allowed the identification of molecular markers of the above-mentioned taxa. The numerical analysis of the obtained results suggests that cv. ‘Broma’ should be classified in the species B. carinatus, not B. willdenowii.

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Study on Biological Materials with Genetic Diversity of Bupleurum marginatum in Northwest of Hubei Province of China Base on ISSR

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.830.463 ◽

2013 ◽

Vol 830 ◽

pp. 463-468

Author(s):

Shi Ming Du ◽

Gang Wang ◽

Yi Mei Liu ◽

Fang Ye ◽

Rong Jin Sun ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Hubei Province ◽

Germplasm Resources ◽

Closely Related Species ◽

Molecular Features ◽

Exploitation And Utilization ◽

Two Samples ◽

Polymorphic Bands ◽

Issr Molecular Markers

To analyze the genetic diversity of Bupleurum marginatum growing in northwest area of Hubei province of china,and provide the molecular identification method for Bupleurum marginatum and basis for its exploitation and utilization. ISSR Molecular markers were used to analyse the genetic relationship between Bupleurum species in northwest area of Hubei province (NAHP) and the Bupleurum chinense DC.. 12 samples of Bupleurum marginatum were amplified to 81 ISSR bands and there were 42 polymorphic bands, the rate of polymorphic bands was 51.9%. 12 samples of Bupleurum marginatum were clustered into one group, two samples of Bupleurum chinense DC. were clustered into another group. The biodiversity and germplasm resources of Bupleurum marginatum in NAHP is abundant and it is beneficial for the development of Bupleurum marginatum. The method of ISSR marker can significantly distinguish Bupleurum marginatum from its closely related species as the molecular features of the species identification and provide basis for distinguishing different germplasm.

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Identification and Genetic Diversity Analysis of Edible and Medicinal Malva Species Using Flow Cytometry and ISSR Molecular Markers

Agronomy ◽

10.3390/agronomy10050650 ◽

2020 ◽

Vol 10 (5) ◽

pp. 650

Author(s):

Iwona Jedrzejczyk ◽

Monika Rewers

Keyword(s):

Flow Cytometry ◽

Molecular Markers ◽

Genome Size ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Size Estimation ◽

Flow Cytometric ◽

First Choice ◽

Issr Molecular Markers

The Malva genus contains species that reveal therapeutic properties and are mostly important in medicine and the functional food industry. Its breeding, cultivation, and utilization are based on proper germplasm/plant identification, which is difficult using morphological features. For this reason, we applied flow cytometry and inter simple sequence repeat polymerase chain reaction (ISSR-PCR) for fast and accurate species identification. Genome size estimation by flow cytometry was proposed as the first-choice method for quick accession screening. Out of the 12 tested accessions, it was possible to identify six genotypes based on genome size estimation, whereas all species and varieties were identified using ISSR markers. Flow cytometric analyses revealed that Malva species possessed very small (1.45–2.77 pg/2C), small (2.81–3.80 pg/2C), and intermediate (11.06 pg/2C) genomes, but the majority of accessions possessed very small genomes. Additionally, this is the first report on genome size assessment for eight of the accessions. The relationships between the investigated accessions showed the presence of two clusters representing malvoid and lavateroid group of species. Flow cytometry and ISSR molecular markers can be effectively used in the identification and genetic characterization of Malva species.

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Genetic similarity between coriander genotypes using ISSR markers

Horticultura Brasileira ◽

10.1590/s0102-05362011000400014 ◽

2011 ◽

Vol 29 (4) ◽

pp. 526-530 ◽

Cited By ~ 1

Author(s):

Roberto de A Melo ◽

Luciane V Resende ◽

Dimas Menezes ◽

Ana Paula A Beck ◽

José Carlos da Costa ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Similarity ◽

Dna Polymorphism ◽

Genetic Characterization ◽

Issr Markers ◽

Whole Genome ◽

Similarity Data ◽

Improvement Programs ◽

Issr Molecular Markers ◽

New Cultivars

With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.

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Systematic position of Paulsilvella in the Lithophylloideae (Corallinaceae, Rhodophyta) confirmed by molecular data

Phytotaxa ◽

10.11646/phytotaxa.190.1.8 ◽

2014 ◽

Vol 190 (1) ◽

pp. 94 ◽

Cited By ~ 6

Author(s):

Beatriz Nogueira Torrano-Silva ◽

Rafael Riosmena-Rodríguez ◽

Mariana Cabral de Oliveira

Keyword(s):

Molecular Markers ◽

Morphological Analysis ◽

Original Description ◽

Molecular Data ◽

Systematic Position ◽

Ssu Rdna ◽

New Hosts ◽

Columnar Cells ◽

Relevant Range ◽

Thallus Surface

New specimens of Paulsilvella huveorum were collected in Brazil at Baía de Ilha Grande, Rio de Janeiro, and Sebastião Gomes reef, Bahia. This new collections represent a relevant range extension and new hosts for the species (Amphiroa beauvoisii, Jania cubensis and non-identified Hydrozoa and Bryozoa) and enabled the first DNA amplifications for Paulsilvella. The systematic position of Pausilvella in the subfamily Lithophylloideae is confirmed based on SSU rDNA, psbA and rbcL molecular markers. Morphologically and anatomically the specimens are similar to the original description in where basal dimerous thalli with monomeric erect branches characterize the genus. But, the analyzed carposporangial conceptacles express the roof position varying from flush or above the thallus surface, the chambers always buried within tiers of columnar cells, suggesting that this feature is variable within the species and might also suggest that P. huveorum and the fossil P. antiqua should be considered as potential synonyms. We do not want to suggest this clump waiting for more collections from different geographical areas in where new data may support our idea. Our results strongly suggest that the subfamily Lithophylloideae urgently needs to be reviewed to delimit genera based on molecular and morphological analysis because monomeric and dimeric thalli organization have evolved several times in the group.

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Genetic divergence in basil cultivars and hybrids

Horticultura Brasileira ◽

10.1590/s0102-053620190208 ◽

2019 ◽

Vol 37 (2) ◽

pp. 180-187

Author(s):

Rodrigo P Alves ◽

Ana Veruska C Silva ◽

Camila S Almeida-Pereira ◽

Tatiana S Costa ◽

Sheila V Alvares-Carvalho ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Variability ◽

Polymorphic Information Content ◽

Dna Amplification ◽

Issr Markers ◽

Economic Importance ◽

Dissimilarity Index ◽

The World ◽

Issr Molecular Markers

ABSTRACT Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic similarity among individuals was calculated, forming four distinct clusters. Most individuals (40.7%) were allocated in cluster I. The polymorphic information content (PIC) (0.89) indicated considerable levels of genetic diversity among genotypes. In this sense, the ISSR markers were efficient in the detection of polymorphisms between the accessions, suggesting the genetic variability of the collection. This result demonstrates the importance of the use of molecular markers and the advantages that this information provides to the breeding of the species.

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APPLICATIONS OF ISSR MARKERS IN STUDIES OF GENETIC DIVERSITY OF Pityrocarpa moniliformis

Revista Caatinga ◽

10.1590/1983-21252020v33n417rc ◽

2020 ◽

Vol 33 (4) ◽

pp. 1017-1024

Author(s):

FRANCIVAL CARDOSO FELIX ◽

KYVIA PONTES TEIXEIRA DAS CHAGAS ◽

CIBELE DOS SANTOS FERRARI ◽

FÁBIO DE ALMEIDA VIEIRA ◽

MAURO VASCONCELOS PACHECO

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Distance ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Shannon Index ◽

Resolving Power ◽

Development Programs ◽

Diversity Studies ◽

Issr Molecular Markers

ABSTRACT Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (Fabaceae) is a native brazilian species with high potential for economic development programs in semiarid regions, mainly related to the production of honey, animal food and firewood. Thus, the objective of this work was to select Inter-Simple Sequence Repeat (ISSR) molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population. For this, 28 ISSR molecular markers were tested, evaluating the total number of loci, polymorphism rate and the Polymorphism Information Content (PIC) for the selected primers, the “Marker Index”, and the “Resolving Power”. Genetic diversity parameters (Nei genetic distance and Shannon index) were evaluated for 30 individuals located in Macaíba, Rio Grande do Norte State, Brazil. Seven primers were selected, which provided 74 loci, with 82% being polymorphic, while the PIC value was 0.344. The Nei genetic distance was 0.244, and the Shannon index was 0.374. Therefore, ISSR molecular markers (UBC 827, 840, 844, 857, 859, 860 and 873) are considered efficient in studying the genetic diversity of populations for the selection of matrices and germplasm banks, and may contribute to the conservation and genetic improvement of P. moniliformis populations.

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Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers

Journal of Botany ◽

10.1155/2016/7910451 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Vasiliy Chokheli ◽

Boris Kozlovsky ◽

Mikhail Sereda ◽

Vladimir Lysenko ◽

Igor Fesenko ◽

...

Keyword(s):

Molecular Markers ◽

Comparative Analysis ◽

Dna Fingerprinting ◽

Wood Species ◽

Dna Markers ◽

Quercus Robur ◽

Botanical Garden ◽

Issr Markers ◽

Sequence Repeat ◽

Issr Molecular Markers

Quercus robur L. is a valuable wood species having long ontogeny and promising to create long-living artificial plantings of recreational and ameliorative purposes in the steppes zone of Russia and other countries. In this work we have performed the genotyping of varieties of Quercus robur L. obtained from collection of Botanical Garden of Southern Federal University using intersimple sequence repeat (ISSR) molecular markers. The most polymorphic ISSR-marker (GA) 8YC was found in the collection. The polymorphic DNA markers identified in the present study can be used for the future breeding works to obtain valuable genotypes of Quercus genus. In addition we have performed DNA fingerprinting of the prospective sample of the variety Q. robur var. tardiflora Czern.

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Genetic variability among cashew hybrids and prediction of superior combinations based on agronomic performance

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2019.v54.00725 ◽

2019 ◽

Vol 54 ◽

Cited By ~ 2

Author(s):

Maraisa Crestani Hawerroth ◽

Patricia do Nascimento Bordallo ◽

Luis Cláudio Pessoa Oliveira ◽

Egnesio Holanda Vale ◽

Francisco das Chagas Vidal Neto ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Variability ◽

Molecular Level ◽

Issr Markers ◽

Phenotypic Characterization ◽

Agronomic Performance ◽

Cashew Nut ◽

Issr Molecular Markers ◽

Rapd And Issr

Abstract: The objective of this work was to evaluate the use of RAPD and ISSR molecular markers to determine the genetic variability among cashew (Anacardium spp.) genotypes, and to indicate possible promising crosses based on cashew genetic variability and phenotypic performance. Ten hybrids - derived from the crosses CCP 76 x BGC 589, CCP 76 x BRS 226, CCP 76 x HAC 276-1, CCP 76 x Embrapa 51, CCP 76 x BRS 253, CCP 76 x HAC222-4, and BRS226 x Embrapa 51 - and their parents were assessed at the molecular level. The hybrids were evaluated for nut yield, mean nut weight, bored nuts, and powdery mildew on nuts (scale 0-4). The RAPD and ISSR markers were efficient in the determinaton of the genetic variability among cashew genotypes, allowing of the grouping of 21 clusters. Associated with the phenotypic characterization of cashew nut for yield, weight, and health, the used markers can efficiently identify possible combinations with higher genetic variability and higher probability of developing transgressive genotypes in segregating populations.

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A Comparative Study of 5S rDNA Non-Transcribed Spacers in Elaeagnaceae Species

Plants ◽

10.3390/plants10010004 ◽

2020 ◽

Vol 10 (1) ◽

pp. 4

Author(s):

Oleg S. Alexandrov ◽

Olga V. Razumova ◽

Gennady I. Karlov

Keyword(s):

Polymerase Chain Reaction ◽

Molecular Markers ◽

Dna Markers ◽

5S Rdna ◽

Chain Reaction ◽

Closely Related Species ◽

Pcr Products ◽

Polymerase Chain ◽

Species Specific ◽

Shepherdia Canadensis

5S rDNA is organized as a cluster of tandemly repeated monomers that consist of the conservative 120 bp coding part and non-transcribed spacers (NTSs) with different lengths and sequences among different species. The polymorphism in the 5S rDNA NTSs of closely related species is interesting for phylogenetic and evolutional investigations, as well as for the development of molecular markers. In this study, the 5S rDNA NTSs were amplified with universal 5S1/5S2 primers in some species of the Elaeagnaceae Adans. family. The polymerase chain reaction (PCR) products of five Elaeagnus species had similar lengths near 310 bp and were different from Shepherdia canadensis (L.) Nutt. and Sh. argentea (Pusch.) Nutt. samples (260 bp and 215 bp, respectively). The PCR products were cloned and sequenced. An analysis of the sequences revealed that intraspecific levels of NTS identity are high (approximately 95–96%) and similar in the Elaeagnus L. species. In Sh. argentea, this level was slightly lower due to the differences in the poly-T region. Moreover, the intergeneric and intervarietal NTS identity levels were studied and compared. Significant differences between species (except E. multiflora Thunb. and E. umbellata Thunb.) and genera were found. Herein, a range of the NTS features is discussed. This study is another step in the investigation of the molecular evolution of Elaeagnaceae and may be useful for the development of species-specific DNA markers in this family.

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