The influence of the preanalytical factors on the results of a Complete Blood Count

2016 ◽  
Vol 51 (4) ◽  
pp. 271-276
Author(s):  
Barbara Kościelniak ◽  
Paulina Kowalczyk ◽  
Anna Manda ◽  
Krystyna Sztefko ◽  
Przemysław Tomasik
Keyword(s):  
Room Temperature ◽  
Blood Count ◽  
Complete Blood Count ◽  
Storage Conditions ◽  
Sample Volume ◽  
Preanalytical Phase ◽  
The Stability ◽  
The Impact ◽  
Adult Volunteers

Background: The credibility of the result of a complete blood count (CBC) is closely connected with the preanalytical phase. Aim of the study: This study evaluated the impact of improper filling of microtubes and assessed the effect of storage conditions on results of CBC. Materials and methods: Blood samples collected into microtubes provided to the laboratory were studied. To determine the effects of sample volume on CBC result, blood from 14 adult volunteers were drawn, and test-tubes were filled with 200, 300, 400, 500, 600 and 700 μl. In the stability studies, overfilled samples stored at ambient temperature were analysed at: 1, 2 and 3 hours after phlebotomy. The analyses were made with the Sysmex KX-21N and XT-1800i analysers. Results: More than 75% of the analysed samples for CBC were incorrectly filled and mainly overfilled (60%). An excess collection of blood volume resulted in the decrease of haemoglobin, haematocrit, red blood cells and mean corpuscular volume as well as in an increase in the number of platelets. The storage of overfilled microtubes for CBC for 3 hours at room temperature had no further effect on the results of this test. Conclusion: Medical staff does not keep the instructions of the manufacturers. It might lead directly to a reduction of the results credibility and a decrease of the quality of the analysis.

scholarly journals Leucopenia during paliperidone treatment in a patient with schizoaffective disorder – a case study

2020 ◽  
Vol 20 (3) ◽  
pp. 207-209
Author(s):  
Bertrand Janota ◽  
◽  
Paulina Michalska ◽  
Anna Szymanik-Kostrzewska ◽  
Katarzyna Janota ◽  
...  
Keyword(s):  
Schizoaffective Disorder ◽  
Dosage Form ◽  
Blood Count ◽  
Leucocyte Count ◽  
Complete Blood Count ◽  
Auditory Hallucinations ◽  
The Impact ◽  
Severe Anxiety

The case study presents a 51-year-old woman diagnosed with a schizoaffective disorder who was initially treated with risperidone. After 7 days of hospitalisation, the patient was discharged at her own request. On the same day, after a few hours, she returned to the emergency room, reporting severe anxiety and auditory hallucinations. Risperidone was replaced by paliperidone. During the treatment, a decrease in leucocyte count after paliperidone administration was observed. During further hospital stay, the treatment was switched to olanzapine in depot form. Correct symptomatic response to the drug used was observed. The leucocyte counts were gradually compensated, which confirms the drug-related cause of previously observed complete blood count abnormalities. The case indicates the possibility of side effects in the treatment with certain drugs, and underlines the impact of the dosage form of the drug being taken on the patient’s quality of life.

Impact of preheparinization and sample volume on routine hematology findings in healthy cats

2020 ◽  
pp. 1098612X2092635
Author(s):  
Gary D Norsworthy ◽  
Audrey K Cook ◽  
Christopher J Lanier
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Hemoglobin Concentration ◽  
Sample Volume ◽  
Mean Cell Volume ◽  
Edta Blood Sample ◽  
Patient Stress ◽  
The Impact ◽  
Specific Volumes ◽  
Edta Blood

Objectives It can be challenging to collect sufficient blood from feline patients for both a biochemical profile and a complete blood count (CBC). The ability to generate accurate hematologic and biochemical data from a single, small (<2 ml) sample could reduce patient stress and improve clinical efficiency. The objective of this study was to determine the impact of preheparinization and/or sample size on routine hematology findings in cats. Methods Blood was collected from 20 healthy cats; measured aliquots were placed directly into tubes containing either EDTA or lithium–heparin (Hep). Within 2 mins, specific volumes were removed from the Hep tubes and placed in additional EDTA tubes. Four distinct sample sizes/types were created from each cat: (1) 1.3 ml EDTA (criterion standard); (2) 0.5 ml EDTA; (3) 1.3 ml Hep + EDTA; and (4) 0.5 ml Hep + EDTA. Three CBCs were performed on each sample using an automated bench-top hematology analyzer. Drops of blood were contemporaneously used to create three air-dried stained slides from each tube. Triplicate results were averaged for statistical analysis; results were compared across all sample types and against the criterion standard. Significance was set at P <0.05. Results Preheparinization did not significantly impact determinations of erythrocyte number, hematocrit, hemoglobin concentration, mean cell volume and neutrophil count. Platelet counts for the non-traditional samples correlated poorly with the criterion standard, although numbers could be effectively estimated using stained slides. Cell morphology was well preserved across all sample types. Conclusions and relevance These results indicate that a 0.5 ml preheparinized EDTA blood sample can generate clinically useful hematologic data (excluding platelet count) in cats, using a bench-top analyzer. Our findings support the collection of a single small (<2 ml) sample that can be used for both biochemical and hematologic analyses. Further studies are needed to verify these findings using other hematology machines and in diseased cats.

Stability of extemporaneously prepared rosuvastatin oral suspension

2017 ◽  
Vol 74 (19) ◽  
pp. 1579-1583 ◽  
Author(s):  
Abdel Naser Zaid ◽  
Rania Shtayah ◽  
Ayman Qadumi ◽  
Mashour Ghanem ◽  
Rawan Qedan ◽  
...  
Keyword(s):  
Relative Humidity ◽  
High Performance ◽  
Room Temperature ◽  
Microbial Contamination ◽  
Active Pharmaceutical Ingredient ◽  
Storage Conditions ◽  
Dissolution Profile ◽  
Stability Studies ◽  
Organoleptic Properties ◽  
The Stability

Abstract Purpose The stability of an extemporaneously prepared rosuvastatin suspension stored over 30 days under various storage conditions was evaluated. Methods Rosuvastatin suspension was extemporaneously prepared using commercial rosuvastatin tablets as the source of active pharmaceutical ingredient. The organoleptic properties, dissolution profile, and stability of the formulation were investigated. For the stability studies, samples of the suspension were stored under 2 storage conditions, room temperature (25 °C and 60% relative humidity) and accelerated stability chambers (40 °C and 75% relative humidity). Viscosity, pH, organoleptic properties, and microbial contamination were evaluated according to the approved specifications. High-performance liquid chromatography was used for the analysis and quantification of rosuvastatin in selected samples. Microbiological investigations were also conducted. Results The prepared suspension showed acceptable organoleptic properties. It showed complete release of rosuvastatin within 15 minutes. The pH of the suspension was 9.8, which remained unchanged during the stability studies. The microbiological investigations demonstrated that the preparation was free of any microbial contamination. In addition, the suspension showed stability within at least the period of use of a 100-mL rosuvastatin bottle. Conclusion Extemporaneously prepared rosuvastatin 20-mg/mL suspension was stable for 30 days when stored at room temperature.

The Impact of Storage Conditions on the Stability of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12 in Human Milk

2017 ◽  
Vol 12 (9) ◽  
pp. 566-569 ◽  
Author(s):  
Anastasia Mantziari ◽  
Juhani Aakko ◽  
Himanshu Kumar ◽  
Satu Tölkkö ◽  
Elloise du Toit ◽  
...  
Keyword(s):  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Storage Conditions ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis ◽  
The Stability ◽  
The Impact

Structural and functional analysis of reconnaissance and fire system and decomposition of its functions and subsystems

2021 ◽  
pp. 38-48
Author(s):  
O. Maistrenko ◽  
O. Karavanov ◽  
A. Shcherba
Keyword(s):  
Functional Analysis ◽  
Fire Damage ◽  
Expert Group ◽  
Expert Evaluation ◽  
Military Conflicts ◽  
Main Factors ◽  
The Stability ◽  
The Impact

It is established that the role of reconnaissance and fire systems in the implementation of enemy fire damage in military conflicts occurring at the beginning of the XXI century. Increased  to 75%. However, it was found that the effectiveness of these systems depends on the quality of their equipment. The main factors that are often not taken into account in the acquisition are the possibility, interoperability, stability of each element, the impact of elements on the stability of each other and the functioning of the RVS as a whole. The analysis of recent research and publications has shown that as of today, the approaches to determining the composition of reconnaissance fire systems taking into account these factors are not justified. Given the above, it was decided to conduct a structural and functional analysis of the reconnaissance fire system and the decomposition of its functions and subsystems. To this end, based on the method of cluster analysis, an algorithm for combining a set of tasks performed by reconnaissance and fire systems into groups has been developed and substantiated. The method of expert evaluation was used to obtain substantiated data on the implementation of such a merger. According to the proposed algorithm, the main tasks were first identified, which involve reconnaissance and fire systems. Subsequently, an expert group was formed and the coefficient of relative competence of each member of the expert group was determined. The experts were then asked to combine the identified tasks according to their characteristics into an arbitrary number of groups. After processing the opinions of experts, 10 characteristic groups were identified. Finally, the experts were asked to combine the obtained groups in such a way that each association consisted of similar objects and the objects of different associations differed significantly. The results of the work revealed the functional interconnectedness of the tasks, processes and subsystems of the reconnaissance and fire system. It is also established that a typical reconnaissance fire system will consist of three subsystems (control, reconnaissance and fire influence), which perform specific functions for each of them.

scholarly journals The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study

Diagnostics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
Lilla Pawlik-Sobecka ◽  
Katarzyna Sołkiewicz ◽  
Izabela Kokot ◽  
Aleksandra Kiraga ◽  
Sylwia Płaczkowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Sample ◽  
Storage Conditions ◽  
Wilcoxon Test ◽  
Reference Ranges ◽  
Sample Storage ◽  
Serum Samples ◽  
The Stability ◽  
The Individual ◽  
The Impact

The present work aims at accessing the stability of biological material stored for diagnostic and scientific purposes. The influence of the temperature, storage time, and cyclic thawing on concentration stability of selected oxidative stress parameters in human serum was investigated. The study group consisted of 20 serum samples collected from healthy volunteers aged 18–52. The parameters whose reference ranges were not determined and to which validated determination methods did not correspond were examined by manual methods (FRAP and AOPP). Automatic methods were used to determine routine laboratory tests (albumin, total protein, bilirubin, uric acid) using the Konelab 20i® analyzer. The samples were stored at various temperatures (room temperature, 4 °C, −20 °C, −80 °C) for max 6 months and were subjected to cyclic thawing at 1 month intervals. In order to check whether any differences between the concentrations of the studied parameters existed when the samples were stored in various conditions, the paired Student t-test or Wilcoxon test and comparison to desirable bias were applied. Based on the obtained results, it was found that the temperature and time of serum sample storage significantly affected the stability of the analyzed parameters and determined different shelf lives of serum samples for oxidative stress examination. Therefore, continuing the investigation concerning the impact of storage conditions on various serum parameters seems justified due to the discrepancy between the individual results obtained by different researchers and the inconsistencies between the results of scientific research and the applicable recommendations.

Influence of exogenous and endogenous factors on the quality of the preanalytical stage of laboratory tests (review of literature)

2020 ◽  
Vol 65 (12) ◽  
pp. 778-784
Author(s):  
D. P. Piskunov ◽  
L. A. Danilova ◽  
A. S. Pushkin ◽  
S. A. Rukavishnikova
Keyword(s):  
Laboratory Testing ◽  
Cell Metabolism ◽  
Endogenous Factors ◽  
Preanalytical Phase ◽  
Exogenous Factors ◽  
The Stability ◽  
The Right ◽  
Needle Diameter ◽  
And Storage

A literature review in the article presents an analysis of the influence of endogenous and exogenous factors on quality of preanalytical phase of laboratory testing. The review shows significance of external and internal factors influencing blood samples at preanalytical phase of laboratory testing. Among the exogenous factors considered: phlebotomy, test tubes for samples, transportation and storage. A number of factors exist at this phase that significantly affect test results. We examined these aspects of phlebotomy process: staff training, disinfectant contamination, needle diameter, needle material contamination. The review considers possible contamination with tube components and the importance of choosing the right anticoagulants and excipients. Transportation and storage of biological samples can be a source of errors at the preanalytical phase of laboratory testing. We analyzed the problem of determining the stability of analytes during storage and aspects of transportation samples by modern means. Among the endogenous factors considered: hemolysis, lipemia, icterricity, cell metabolism.. Hemolysis is one of the most frequent consequences of errors at the preanalytical phase. We analyzed importance of choosing a method for identifying hemolized tubes and the heterogeneity of bias results on different analytical systems. The review shows contribution of various classes of lipoproteins to turbidity of sample, possible preanalytical errors and impact on analytical tests. We examined possible effects of high bilirubin concentrations on analyte measurements. In the review, we also examined metabolism of some cells and its effect on samples.

scholarly journals Stability of Screening Compounds in Wet DMSO

2008 ◽  
Vol 13 (10) ◽  
pp. 999-1006 ◽  
Author(s):  
Caroline Engeloch ◽  
Ulrich Schopfer ◽  
Ingo Muckenschnabel ◽  
Francois Le Goff ◽  
Hervé Mees ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Medicinal Chemistry ◽  
Storage Conditions ◽  
Pragmatic Approach ◽  
Analytical Quality ◽  
The Past ◽  
Effect Of Water ◽  
The Impact

The impact of storage conditions on compound stability and compound solubility has been debated intensely over the past 5 years. At Novartis, the authors decided to opt for a storage concept that can be considered controversial because they are using a DMSO/water (90/10) mixture as standard solvent. To assess the effect of water in DMSO stocks on compound stability, the authors monitored the purity of a subset of 1404 compounds from ongoing medicinal chemistry projects over several months. The study demonstrated that 85% of the compounds were stable in wet DMSO over a 2-year period at 4 °C. This result validates the storage concept developed at Novartis as a pragmatic approach that takes advantage of the benefits of DMSO/water mixtures while mediating the disadvantages. In addition, the authors describe how purity data collected over the course of the chemical validation of high-throughput screening actives are used to improve the analytical quality of the Novartis screening deck. ( Journal of Biomolecular Screening 2008:999-1006)

Thermal and Chemical Stability of Micron Aluminum Powders Subjected to Gaseous Water

2016 ◽  
Vol 712 ◽  
pp. 241-245
Author(s):  
Sergey V. Zmanovskiy ◽  
Alexander M. Gromov ◽  
Valentina V. Smirnova ◽  
Vadim F. Petrunin ◽  
Jin Chun Kim
Keyword(s):  
Room Temperature ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Aluminum Powders ◽  
X Ray ◽  
The Stability ◽  
Heating Up ◽  
The Impact ◽  
Thermal And Chemical Stability ◽  
Composition Of Products

The paper studies the impact of gaseous water on the stability of micron aluminum powders in time at room temperature using the method of gravimetric analysis. The stability was studied using methods of thermal analysis during heating up to 1200 °С in air. The composition of products was analyzed using X-ray diffraction analysis. It was found out that the stability of micron aluminum powders depends on partial pressure of water vapor: the increase of pressure results in decreased stability of powders. The work gives recommendations for storing micron aluminum powders.

scholarly journals Physicochemical Characteristics and Oil Keeping Quality of Two Cultivars of Sunflower (Helianthus annuus) Seeds

2019 ◽  
pp. 1-10
Author(s):  
A. Kafi ◽  
S. Gheyasuddin ◽  
M. H. Rashid
Keyword(s):  
Crude Protein ◽  
Room Temperature ◽  
Physicochemical Characteristics ◽  
Storage Conditions ◽  
Sunflower Seeds ◽  
Peroxide Values ◽  
Keeping Quality ◽  
Edible Purpose ◽  
Iodine Values

The work was conducted on sunflower seeds of two cultivars namely ‘Kironi’ and ‘Hysun-46’. Proximate composition of the seeds, chemical characteristics and fatty acid composition of the oils, and its keeping quality at different storage conditions were studied. Moisture content of Kironi seeds was nearly twice than Hysun-46 (8.03 vs 4.46%). Crude fat in Hysun-46 seeds was somewhat higher than Kironi. Kironi had significantly higher crude protein whereas Hysun-46 contained significantly higher percent of starch than Kironi (7.05 vs 3.90%). Physical characteristics of oil such as viscosity, colour and transparency changed with time during storage; specific gravity and smoking temperature, however, remained unchanged. Acid values of the freshly extracted oil from Hysun-46 were unexpectedly high (98.75). Iodine values were found to be higher in Kironi than Hysun-46, so the former had greater proportion of unsaturation. Saponification values of the oils decreased with the time in open vessel, in amber coloured bottle at 4°C and also in boiled oil kept at room temperature. However, these values registered an increase in oils stored in closed vessel and amber coloured bottle at room temperature. Peroxide values increased in oils under all conditions except in amber bottle at 4°C. The ratio of linoleic acid to oleic acid in Kironi (2.3:1) was higher than that in Hysun-46 (1.9:1), indicating that Kironi had more semidrying capacity and suitable for edible purpose. The freshly extracted oil had attractive appearance. Between the two oil samples, Kironi seems somewhat superior to Hysun-46.

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