Metabolic Profile of and Antimicrobial Activity in the Aerial Part of Leonurus turkestanicus V.I. Krecz. et Kuprian. from Kazakhstan

2017 ◽  
Vol 100 (6) ◽  
pp. 1700-1705 ◽  
Author(s):  
Olga Sermukhamedova ◽  
Krzysztof Kamil Wojtanowski ◽  
Jarosław Widelski ◽  
Izabela Korona-Głowniak ◽  
Hosam O Elansary ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Iridoid Glycosides ◽  
Chloroform Extract ◽  
Bacillus Species ◽  
Alcoholic Extract ◽  
Aerial Parts ◽  
Nitrogen Containing Compounds ◽  
First Time ◽  
Tof Ms

Abstract An HPLC quadrupole time-of-flight (Q-TOF) MS method was developed for the identification of secondary metabolites in Leonurus turkestanicus V.I. Krecz. et Kuprian. Ethanolic and chloroform extracts from the plant’s aerial parts were tested. A total of 16 compounds (iridoid glycosides, phenylpropanoids, flavonoids, and nitrogen-containing compounds, as well as diterpene acetate derivatives) were identified and tentatively characterized based on (or using) their retention times and UV and Q-TOF-MS data. Previously reported aucubin (1),6-deoxy-8-acetylharpagid(2), and stachydrine(13, 15)and homostachydrine isomers(14, 16)were identified, along with a lavandulifolioside isomer(3),verbascoside(4),rutin(5),3-O-kaempferol rutinoside (6), and an unknown diterpene acetate(8).Compounds3–6were detected for the first time in this plant. Additionally. antimicrobial activity was evaluated. No significant differences were found between ethanolic and chloroform extracts of L. turkestanicus; however, the alcoholic extract showed stronger antifungal activity [minimal inhibitory concentration (MIC) of 2.5–5 mg/mL], whereas the chloroform extract showed stronger activity against the tested spore-forming Bacillus species (MIC 1.25–2.5 mg/mL).

scholarly journals DELPHINIUM LEPTOCARPUM NEVSKI AND DELPHINIUM PARADOXSUN BGE AS POTENTIAL SOURCES FOR PEST CONTROL IN CEREALS AND LEGUMES

2020 ◽  
Vol 2 (10(79)) ◽  
pp. 71-74
Author(s):  
S. Turaeva ◽  
U. Kurbanov ◽  
E. Kurbanova ◽  
S. Allakulova
Keyword(s):  
Plant Extracts ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Chloroform Extract ◽  
Alcoholic Extract ◽  
Lc50 Value ◽  
Aerial Parts ◽  
Potential Sources ◽  
Insecticidal Toxicity

In the present study, the insecticidal activity of plant extracts from the aerial parts of Delphinium leptocarpum Nevski and Delphinium paradoxsun Bge against two major important legume insect pests, cowpea weevils (Callosobruchus maculates) and the rice weevil (Sitophilus oryzae), was studied. According to the screening results, the insecticidal toxicity of the chloroform extract of plant D. leptocarpum N. showed LC50 value of 0,70 mg / mL for adult C. maculates, 0,81 mg / mL for S. oryzae, respectively. The toxicity of the alcoholic extract of D. paradoxsun B. is LC50 0,35 mg / mL for adult C. maculates, 0,39 mg/ mL for S. oryzae, respectively. Exposure to the chloroform extract of D. leptocarpum N. at concentration of 5-10 mg / mL for 24 hours resulted in 83.0100% mortality. After 48 h exposure, the pest mortality was 100%. Under the influence of an alcoholic extract of D. paradoxsun B. at dose of 5-10 mg /mL, the mortality of pests reached 99-100%. The probable use of extracts of D. leptocarpum N. and D. paradoxsun B.as bioinsecticides is discussed here.

scholarly journals Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4118 ◽  
Author(s):  
Noha Fathallah ◽  
Marwa M. Raafat ◽  
Marwa Y. Issa ◽  
Marwa M. Abdel-Aziz ◽  
Mokhtar Bishr ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Antibiofilm Activity ◽  
Moderate Activity ◽  
Ammi Majus ◽  
First Time

Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin )4), and flavoglaucin (5). Structure elucidation was carried out using (1H- and 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

scholarly journals Antimicrobial Properties of Bridelia Scandens against Oral Pathogens: In vitro study

2018 ◽  
Vol 08 (02) ◽  
pp. 032-042 ◽  
Author(s):  
Sachidananda Mallya ◽  
Shrikara Mallya ◽  
Venkatakrishna Rao
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Aqueous Extract ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Alcoholic Extract ◽  
Sensitivity Testing ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Oral Pathogens

Abstract Objective: It has been well documented that traditional medicinal plants confer considerable antimicrobial activity against various microorganisms. Species of the genus Bridelia scandens is reported to be used traditionally for treating various oral diseases. Its effect against intestinal and other systemic pathogens have been reported However the beneficial effects of this plant materials against oral pathogens is not evaluated. Therefore we have done the present investigation. Aim is to study the antimicrobial properties of alcoholic and aqueous extract of Bridelia scandens against selected oral pathogens by various microbiological assays. Methods: The leaves after identification and authentication by a Botanist were collected, air dried, pulverized to fine powder using household blender. The water extract was prepared using cold percolation method and alcoholic extract was prepared in Soxhlet apparatus by using ethyl alcohol. The test organisms like Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius Lactobacillus acidophilus and Candida albicans were obtained from Department of Microbiology Yenepoya Medical College, Mangalore. Antimicrobial activity is being determined by various Microbial assays like Kirby Bauer antibiotic sensitivity testing, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) Results: From our study, it was found that all tested organisms were sensitive to both water and alcoholic extract of leaves of Bridelia scandens at varying concentrations.( 200 mg/ml and 400 mg/ml ) with zone of inhibition ranging from 20 mm to 48 mm .Even the Minimum Inhibitory Concentration ( MIC ) was less than 100 mg/ml in most of the organism indicating that extract of leaves of Bridelia scandens is most effective against the oral pathogens. The alcoholic extract is better than aqueous extract. Conclusion: The extract of leaves of Bridelia scandens is highly effective against selected oral pathogens

scholarly journals Flavonoids from Stellaria nemorum and Stellaria holostea

2015 ◽  
Vol 10 (3) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Elena Ancheeva ◽  
Georgios Daletos ◽  
Rini Muharini ◽  
Wen Han Lin ◽  
Leonid Teslov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Acid Hydrolysis ◽  
Two Dimensional ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Aerial Parts ◽  
Crude Extracts ◽  
First Time

Stellaria nemorum L. and S. holostea L. (Caryophyllaceae) were investigated for their flavonoids. The new flavonoid 6- C-[(α-arabinopyranosyl)-(1→2)- O-β-xylopyranosyl]apigenin (1) and the four known C-glycosides, 6- C-[(α-arabinopyranosyl)-(1→2)- O-β-glucopyranosyl]apigenin (2), apigenin 6- C-β-galactopyranoside-8- C-β-glucopyranoside (3), apigenin 6- C-β-glucopyranoside-8- C-α-arabinopyranoside (4), and apigenin 6- C-β-glucopyranoside-8- C-β-xylopyranoside (5) were isolated from the aerial parts of S. nemorum for the first time. Furthemore, five known flavonoids, 3,5,7-trihydroxy-3′,5′-dimethoxyflavone (9), diosmetin 6- C-β-glucopyranoside (8), schaftoside (4), isoorientin (6) and orientin (7) were obtained from the aerial parts of S. holostea. Compounds 4, 8 and 9 are reported for the first time from this species. The structures of all isolated compounds were unambiguously elucidated by one- and two- dimensional NMR and mass spectral analysis, by acid hydrolysis, as well as by comparison with literature data. The crude extracts of the investigated species exhibited antimicrobial activity against Staphylococcus aureus, while none of the isolated compounds was found to be active.

scholarly journals Synthesis, Antimicrobial Properties, and Inhibition of Catalase Activity of 1,4-Naphtho- and Benzoquinone Derivatives Containing N-, S-, O-Substituted

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Semih Kurban ◽  
Nahide Gulsah Deniz ◽  
Cigdem Sayil ◽  
Mustafa Ozyurek ◽  
Kubilay Guclu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Catalase Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Significant Inhibition ◽  
Inhibition Activity ◽  
Low Concentrations ◽  
Test Culture ◽  
First Time

A series of new 1,4-naphtho- and benzoquinone derivatives possessing N-, S-, O-substituted groups which has not been reported yet has been synthesized from 2,3-dichloro-1,4-naphthoquinone 1 and 2,3,5,6-tetrachlorocyclohexa-2,5-diene-1,4-dione 15 involving a Michael addition. In the synthesized compounds, antimicrobial activity at low concentrations against Escherichia coli B-906, Staphylococcus aureus 209-P, and Mycobacterium luteum B-917 bacteria and Candida tenuis VKM Y-70 and Aspergillus niger F-1119 fungi in comparison with controls was identified. 2-Chloro-3-((2-(piperidin-1-yl)ethyl)amino)naphthalene-1,4-dione 3g and 2,5-dichloro-3-ethoxy-6-((2,4,6-trifluorophenyl)amino)cyclohexa-2,5-diene-1,4-dione 17 were the most potent, with a minimum inhibitory concentration value of 15.6 μg/mL against test-culture M. luteum and S. aureus, respectively. Furthermore, in this work, a catalase activity of benzo- and naphthoquinone derivatives was examined for the first time. The catalase activity of benzo- and naphthoquinone derivatives was determined, showing that compound 3g had significant inhibition activity for catalase enzyme.

Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial Parts

2020 ◽  
Vol 10 (1) ◽  
pp. 15-19
Author(s):  
Turgut Taşkın ◽  
Yeliz Akkılıç ◽  
Berna Özbek Çelik ◽  
İsmail Şenkardeş ◽  
Özlem B. Özakpınar
Keyword(s):  
Antimicrobial Activity ◽  
Toxic Effect ◽  
Cytotoxic Activity ◽  
Normal Cell ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Normal Cell Line ◽  
Aerial Parts

Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant’s different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and flavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using microdilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (CUPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicans strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.

scholarly journals Phytochemical studies on the aerial parts of Sarcococca hookeriana (Baillon) of Nepalese origin

1970 ◽  
Vol 21 ◽  
pp. 8-15
Author(s):  
Narayan P. Rai ◽  
Bimala Lama ◽  
Shiva P. Subedi ◽  
W. C. Taylor ◽  
M. D. Manandhar
Keyword(s):  
Spectral Analysis ◽  
Oleanolic Acid ◽  
1H Nmr ◽  
13C Nmr ◽  
Chemical Society ◽  
Chloroform Extract ◽  
Hmbc Spectrum ◽  
Aerial Parts ◽  
Phytochemical Studies ◽  
First Time

Two known pregnane type of alkaloids, axillaridine A and spiropachysine, and an unidentified pregane alkaloid along with oleanolic acid and stigmasteryl glucoside have been isolated from chloroform extract of the aerial parts of Sarcococca hookeriana. The structures of the alkaloids are established on the basis of spectral analysis of 1H-NMR, 13C-NMR, HMQC, COSY and HMBC spectrum. All compounds were isolated for the first time from S. hookeriana. So far, species of Sarcococca was not reported to contain spiropachysine which was initially reported as a novel alkaloid from Pachysandra terminalis SIEB. et ZUCC.(Buxaceae).DOI: 10.3126/jncs.v21i0.215Journal of Nepal Chemical Society Vol.21 2006 pp.8-15

Lignans, Phenylpropanoids and Polyacetylenes from Chaerophyllum aureum L. (Apiaceae)

2003 ◽  
Vol 58 (7-8) ◽  
pp. 553-557 ◽  
Author(s):  
Judith M. Rollinger ◽  
Christian Zidorn ◽  
Michael J. Dobner ◽  
Ernst P. Ellmerer ◽  
Hermann Stuppner
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Activity ◽  
Nmr Spectroscopy ◽  
Plant Material ◽  
High Resolution Mass Spectrometry ◽  
Active Principle ◽  
Aerial Parts ◽  
Tlc Plates ◽  
First Time ◽  
Resolution Mass

Abstract Sub-aerial parts of Chaerophyllum aureum L. yielded two polyacetylenes, falcarinol (1), falcarindiol (2), three lignans, namely nemerosin (3), deoxypodorhizone (4), deoxypodo-phyllotoxin (5), two phenylpropanoids, 1′-hydroxymyristicin (6) and its angeloyl ester (7). Compounds 6 and 7 were isolated for the first time from plant material and their structures were elucidated by means of extensive 1- and 2-dimensional NMR spectroscopy and high resolution mass spectrometry. In bioautographic tests on TLC plates the dichloromethane extract showed a significant antimicrobial activity. Falcarindiol was identified as the main active principle whereas the phenylpropanoids and lignans showed no activity.

scholarly journals Phenylethanoid and Iridoid Glycosides from Veronica Chamaedrys L.

2008 ◽  
Vol 3 (2) ◽  
pp. 101-104
Author(s):  
Alexandra Marchenko ◽  
Pavel Kintia ◽  
Natalia Mashcenco ◽  
Carla Bassarello ◽  
Sonia Piacente ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Iridoid Glycosides ◽  
Iridoid Glycoside ◽  
Phenylethanoid Glycosides ◽  
Aerial Parts ◽  
First Time

Three phenylethanoid glycosides (1, 2, 3) and one iridoid glycoside (4) were isolated from aerial parts of Veronica chamaedrys L. (Scrophulariaceae) for the first time. On the basis of spectral analysis, the structures of these compounds were determined to be acteoside (3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside) (1), ehrenoside (3,4-dihydroxy-β-phenylethoxy-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside) (2), chamaedroside (3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-α-L-arabinopyranoside) (3) and aucuboside (4).

scholarly journals Chemical composition of endemic Centaurea austro-anatolica and studies of its antimicrobial activity against multi-resistant bacteria

2009 ◽  
Vol 59 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Aysel Ugur ◽  
Nurdan Sarac ◽  
Ozgur Ceylan ◽  
M. Duru
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Aerial Parts ◽  
Multiresistant Bacteria ◽  
Paper Disc

Chemical composition of endemicCentaurea austro-anatolicaand studies of its antimicrobial activity against multi-resistant bacteriaHexane, chloroform, ethyl acetate and ethanolic extracts of the aerial parts ofCentaurea austro-anatolicaHub.-Mor. (Asteraceae) were evaluated against microorganisms, including multiresistant bacteria, using a paper disc diffusion method. The chloroform extract exhibited significant antibacterial activity toward all bacteria tested. The chemical composition of the chloroform extract was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major compounds of the extract were caryophyllene oxide (21.32 %), spathulenol (10.86 %),n-tricosanol (9.58 %) and geranyl isovalerate (8.71 %).

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