Antioxidant and anticancer activities of methanolic extract of Trollius chinensis Bunge

The plant Indigofera aspalathoides is a traditional medicine with tremendous therapeutic potential which finds it use in treatment of various ailments such as antibacterial, antioxidant, anti-inflammatory, antidiabetic, and anticancer activities. There are no reports that related to the use of this plant in treating patients with Alzheimer’s disease (AD). Hence present study was aimed to scientifically evaluate the neuroprotective effect of the methanolic extract of Indigofera aspalathoides against scopalamine induced Alzheimer’s disease in experimental rats using behavioral tests like elevated plus maze, Y-maze, and rota-rod tests. In addition to this, biochemical evaluation for acetylcholinesterase activity and histopathological evaluation of brain were done. The results suggests that methanolic extract Indigofera aspalathoides (200mg/kg B.wt and 400mg/kg B.wt) used in this study shows significant improvement of various behavioral parameters like locomotion, anxiety, memory, motor integrity and coordination etc when compared to control group. MEIA inhibited brain AChE enzyme, thereby elevating Ach concentration in brain homogenate and ultimately improved memory of rats. Further, more or less normal histological structure of the hippocampus and all amyloid plaques and neurofibrillary tangles that are formed under the influence of scopolamine disappeared in the rats pretreated with MEIA (200mg/kg B.wt and 400mg/kg B.wt). It can be concluded that our results strongly support the anti-Alzheimer’s potential of the methanolic extract of the plant I.aspalathoides and its use in traditional medicine.

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Anticancer Activities of the Methanolic Extract from Lemon Leaves in Human Breast Cancer Stem Cells

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2015.034 ◽

2015 ◽

Vol 58 (3) ◽

pp. 219-226 ◽

Cited By ~ 1

Author(s):

Jeong Yong Moon ◽

Linh Thi Thao Nguyen ◽

Ho Bong Hyun ◽

Ahmed Osman ◽

Minwhan Cho ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Human Breast Cancer ◽

Methanolic Extract ◽

Breast Cancer Stem Cells ◽

Human Breast ◽

Anticancer Activities

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Erratum to: Anticancer Activities of the Methanolic Extract from Lemon Leaves inHuman Breast Cancer Stem Cells

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2016.031 ◽

2016 ◽

Vol 59 (2) ◽

pp. 171-171

Author(s):

Jeong Yong Moon ◽

Linh Thi Thao Nguyen ◽

Ho Bong Hyun ◽

Ahmed Osman ◽

Kwang SeokAhn ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Methanolic Extract ◽

Breast Cancer Stem Cells ◽

Anticancer Activities

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Antiproliferative Activity and Apoptotic Efficiency of Syzygium cumini Bark Methanolic Extract against EAC Cells In Vivo

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200811122137 ◽

2020 ◽

Vol 20 ◽

Author(s):

Mst. Ayesha Siddika ◽

Plabon K. Das ◽

Saharia Y. Asha ◽

Suraiya Aktar ◽

Abu Rahyan M. Tareq ◽

...

Keyword(s):

Anticancer Activity ◽

Antiproliferative Activity ◽

Methanolic Extract ◽

Chemical Constituents ◽

Antioxidant Properties ◽

In Vitro Assays ◽

Syzygium Cumini ◽

Anticancer Activities ◽

Eac Cells

Background: Syzygium cumini, one of the evidence-based traditional medicinal plant used in the treatment of various ailments. Objectives: Herein, the antioxidant property and anticancer property of Syzygium cumini against Ehrlich Ascites Carcinoma (EAC) were examined in search of effective chemotherapeutics. Methods: In vitro assays, phytochemical and chromatographic analysis were used to determine antioxidant properties and chemical constituents of Syzygium cummini bark methanolic extract (SCBME). Functional assays were used to measure the anticancer activity of SCBME. Fluorescence microscopy and RT-PCR were used to examine morphological and molecular changes of EAC cells followed by SCBME treatment. Results: Phytochemical and GC–MS analysis confirmed the presence of compounds with antioxidant and anticancer activities. Accordingly, we have noted a strong antioxidant activity of SCBME with an IC50 value of 10μg/ml. Importantly, SCBME exerted a dose-dependent anticancer activity with significant inhibition of EAC cell growth (71.08 ± 3.53%; p<0.001), reduction of tumour burden (69.50%; p<0.01) and increase of life span (73.13%; p<0.001) of EACbearing mice at 75mg/kg/day. Besides, SCBME restored the blood toxicity towards normal in EAC-bearing mice (p<0.05). SCBME treated EAC cells showed apoptotic features under a fluorescence microscope and fragment DNA in DNA laddering assay. Moreover, up-regulation of the tumour suppressor p53 and pro-apoptotic Bax and down-regulation of NF-κB and anti-apoptotic Bcl-2 genes, implied induction of apoptosis followed by SCBME treatment. Conclusion: The antiproliferative activity of SCBME against EAC cells is likely due to apoptosis, mediated by regulation of p53 and NF-κB signalling. Thus, SCBME can be considered as a useful resource in cancer chemotherapy.

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V. NEGUNDO, L. CAMARA AND B. VARIEGATA PLANTS LEAF EXTRACT EXHIBIT CONSIDERABLE IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITIES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.17721 ◽

2017 ◽

Vol 9 (5) ◽

pp. 227

Author(s):

Nutan Badgujar ◽

Kinnari Mistry ◽

Jagdish Patel

Keyword(s):

Anticancer Activity ◽

Methanolic Extract ◽

Assay Method ◽

Human Neuroblastoma Cell ◽

Anticancer Activities ◽

Human Neuroblastoma ◽

Antioxidant Power ◽

Methanolic Extracts ◽

Ic50 Value

Objective: The study was planned to investigate antioxidant and anticancer activities with the preliminary phytochemical analysis of methanolic extracts of Vitex negundo (V. negundo), Lantana camara (L. camara) and Bauhania variegata (B. variegata) plants leaf extracts.Methods: Phytochemical evaluation was performed for all the extracts, as per the standard methods. In vitro antioxidant activities were performed by using DPPH (2,2-Diphenyl-1-Picrylhydrazyl), ABTS (2, 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid) and FRAP (Ferric reducing antioxidant power assay) method and compared with standard antioxidants. The anticancer activity of plant extract was assessed using MTT colorimetric assay.Results: The study of preliminary phytochemical proved the existence of alkaloids, flavonoids and phenolic types of phytochemicals in high amount. Methanolic extract of L. camara shows minimum IC50 value for DPPH assay (48.75±2.34 µg/ml) and FRAP assay (274.66±3.65 µg/ml). In ABTS assay B. variegata extract exhibit minimum IC50 value (60.48±3.01 µg/ml). Lower the IC50 value of extract, higher the effectiveness of the plant. Methanolic extract of all plants methanolic extracts showed anticancer activity against SH-SY-5Y cells (human neuroblastoma cell) but V. negundo was more effective against SH-SY-5Y cells with IC50 value (209 µg/ml) compared to remaining extracts.Conclusion: The current finding accomplished the in vitro activities, so that plant could be a superior source of antioxidant and anticancer drugs. But further in vivo assessment was needed before adding it into the pharma industry.

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Erratum to: Anticancer Activities of the Methanolic Extract from Lemon Leaves in Human Breast Cancer Stem Cells

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2015.062 ◽

2015 ◽

Vol 58 (4) ◽

pp. 389-389

Author(s):

Jeong Yong Moon ◽

Linh Thi Thao Nguyen ◽

Ho Bong Hyun ◽

Ahmed Osman ◽

Kwang Seok Ahn ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Human Breast Cancer ◽

Methanolic Extract ◽

Breast Cancer Stem Cells ◽

Human Breast ◽

Anticancer Activities

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High-Resolution UPLC-MS Profiling of Anthocyanins and Flavonols of Red Cabbage (Brassica oleracea L. var. capitata f. rubra DC.) Cultivated in Egypt and Evaluation of Their Biological Activity

Molecules ◽

10.3390/molecules26247567 ◽

2021 ◽

Vol 26 (24) ◽

pp. 7567

Author(s):

Khaled Ahmed Mansour ◽

Sherifa Fahmy Moustafa ◽

Soad Mohamed Abdelkhalik

Keyword(s):

High Resolution ◽

Brassica Oleracea ◽

Methanolic Extract ◽

Statistical Significance ◽

Anticancer Activities ◽

Red Cabbage ◽

Ionization Mode ◽

Negative Ionization ◽

First Time ◽

Brassica Oleracea L

In this paper, biological investigations and a high-resolution UPLC-PDA-ESI-qTOF-HRMS technique were employed for Brassica oleracea L. var. capitata f. rubra DC. (red cabbage) of the family Brassicaceae (Cruciferae), cultivated in Egypt, for the first time. The positive ionization mode is usually performed to identify anthocyanins. However, this technique cannot differentiate between anthocyanins and corresponding non-anthocyanin polyphenols. Thus, the negative ionization mode was also used, as it provided a series of characteristic ions for the MS analysis of anthocyanins. This helped in identifying five kaempferol derivatives for the first time in red cabbage, as well as nine—previously reported—anthocyanins. For the biological investigations, the acidified methanolic extract of fresh leaves and the methanolic extract of air-dried powdered leaves were examined for their antioxidant, antimicrobial, and anticancer activities. The freshly prepared phenolic extract was proven to be more biologically potent. Statistical significance was determined for its anticancer activity in comparison with standard doxorubicin.

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GC-analysis, and Antioxidant, Anti-inflammatory, and Anticancer Activities of Some Extracts and Fractions of Linum usitatissimum

Current Bioactive Compounds ◽

10.2174/1573407216666200206095954 ◽

2020 ◽

Vol 16 (9) ◽

pp. 1306-1318

Author(s):

Eman A. Morsi ◽

Hend O. Ahmed ◽

Heba Abdel-Hady ◽

Mortada El-Sayed ◽

Mohamed A. Shemis

Keyword(s):

Ethyl Acetate ◽

Anticancer Activity ◽

Cell Line ◽

Linum Usitatissimum ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Anticancer Activities ◽

Anti Inflammatory ◽

Phenolic And Flavonoid

Context: Linum usitatissimum (Linn); known as Flaxseed, is one of the most important medicinal plants traditionally used for health benefits and also as nutritional purposes. Objective: Estimation of total phenolic and flavonoid contents, and evaluation of the antioxidant, antiinflammatory and anticancer activities have been performed on hexane, ethyl acetate, n-butanol, and methanol extracts and also on fractions of methanol extract (hexane, ethyl acetate, and n-butanol). Materials and Methods: Phenolic and flavonoid contents were detected using spectrophotometric and colorimetric assays. Antioxidant and anti-inflammatory activities were estimated in-vitro. Anticancer activity of extracts was tested on Hepatocellular carcinoma cell line (HepG2) and breast cancer cell line (MCF7). Results: The methanolic extract and its ethyl acetate fraction showed higher contents of total phenols and flavonoids. Also, the methanolic extract showed a higher antioxidant activity. The butanolic and ethyl acetate fractions at concentration 500μg/ml yielded a higher percent of inhibition of protein denaturation; 87.9% and 90%, respectively. The ethyl acetate fraction and the methanolic extract showed an obvious anticancer activity against HepG2 and MCF7 (IC50=60±0.24 and 29.4±0.12μg. ml-1) and (IC50=94.7±0.21 and 227±0.48μg. ml-1), respectively. The GC-MS analysis showed that the methanolic extract had 32 compounds whereas the ethyl acetate and butanol fractions contained 40 and 36 compounds, respectively. Conclusion: Flaxseed contains different biologically active compounds that have been found to possess various activities, which can protect the human body against several diseases.

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Methanolic Bark Extract of Abroma augusta (L.) Induces Apoptosis in EAC Cells through Altered Expression of Apoptosis Regulatory Genes

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/9145626 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Masum Miah ◽

Ajmeri Sultana Shimu ◽

Shafi Mahmud ◽

Farjana-Binta Omar ◽

Ratna Khatun ◽

...

Keyword(s):

Hemolytic Activity ◽

Methanolic Extract ◽

Apoptotic Cell ◽

Docking Simulation ◽

Assay Method ◽

Bark Extract ◽

Anticancer Activities ◽

Altered Expression ◽

Eac Cells

Abroma augusta (L.), one of the herbal medicinal plants, is widely used for treatment of various maladies. The present study was initiated to determine the antioxidant, hemolytic, cytotoxicity, and anticancer activities of methanolic extract from the bark of the plant. The phytochemical screening was done by analyzing different phytochemicals present in the extract. We observed the presence of alkaloids, steroids, terpenoids, flavonoids, reducing sugars, and glycosides in the bark extract which showed the highest antioxidant capacity. Antioxidant potential of the methanolic extract was evaluated in vitro by DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assay method. This extract showed prominent scavenging activity with IC50 value of 38.65 μg/ml. The hemolytic activity of the extract was evaluated at concentrations ranging from 250 to 1000 μg/ml. It was observed that the extract induced hemolysis percentage of 9.41% to 4.1%, which implies that the extract has no potent hemolytic activity. Cytotoxicity and anticancer activities were observed on Ehrlich ascites carcinoma (EAC) cells. In addition, the bark showed promising cytotoxicity with IC50 value of 329.41 μg/ml, and the study indicated that the extract was capable of inhibiting EAC cell growth by 75.5% when administered at 100 mg/kg/day body weight intraperitoneally for five consecutive days to Swiss albino mice. Morphological change of apoptotic cell was determined by fluorescence and optical microscopy. DNA fragmentation is another marker for apoptosis, and the bark extract-treated EAC cells showed smeared and fragmented DNA bands. Apoptosis correlated well with the upregulation of p53 and Bax and also with the downregulation of NF-κB and Bcl-2. Furthermore, activity and interaction of two A. augusta compounds were tested through molecular docking simulation study. In conclusion, our results suggest that A. augusta bark has the potential to be considered as an anticancer agent.

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