scholarly journals Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Roberta Heloísa de Paula Magalhães ◽  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinicius Abadia Ventura ◽  
Hellen Regina Fernandes Batista-Ventura ◽  
Carlos Frederico de Souza Castro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Refractive Index ◽  
Essential Oil ◽  
Relative Density ◽  
Optical Rotation ◽  
Artemia Salina ◽  
Cytotoxic Activities ◽  
Chemical Profile ◽  
Radical Reduction ◽  
Antibacterial Potential

Cochlospermum regium is a shrub plant species from the Cerrado domain used in traditional medicine. This study aimed to evaluate the chemical profile and antioxidant, antibacterial and cytotoxic activities on Artemia salina from the essential oil of fresh leaves and xylopodium. Fresch leaves and xylopodium of C. regium were collected in an area of ​​Cerrado domain in Goiás State, Brazil, 2021. The essential oil was obtained by hydrodistillation, the yield was quantified and the chemical profile determined by gas chromatography with mass spectrometry (GC-MS). Physicochemical analyzes were carried out for organoleptic analysis (color and appearance), solubility, relative density (g mL-1), refractive index, optical rotation (αD), antioxidant activity in DPPH radical reduction (IC50 µL mL-1), antibacterial activity on Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Salmonella serovar Enteritidis and Salmonella serovar Thyphymurium by the disc method (mm), and cytotoxicity bioassay on Artemia salina (LC50 µg mL-1). The major compounds for the essential oil of fresh leaves were viridiflorol 10.21%, Copaen-4-α-ol <β>, longiborneol 9.07 and β-bisabolene 11.48%, and for the essential oil of xylopodium β-selinene 26.17%, aromadendrene 8.66 % and thujopsene 8.09%. The yield was 0.58 and 0.33%, color slightly yellow and yellow for fresh leaves and xylopodium, respectively. Positive solubility, refractive index of 1.3468 and 1.3347, optical rotation +48.8 and +21.5, relative density 0.932 and 0.936 g mL-1, antioxidant activity IC50 = 47.65 and 111.16 µL mL-1 for fresh leaves essential oil and xylopodium, respectively. The essential oil from fresh leaves showed high antibacterial potential for all strains, as well as for cytotoxic activity on A. salina with LC50 = 90.17 and 625.08 µg mL-1, respectively.

scholarly journals Caracterização química e atividades antioxidante e antifúngica do óleo essencial das flores de [Cochlospermum regium (Mart. ex Schrank.) Pilger] (Bixaceae)

2020 ◽  
Vol 1 (52) ◽  
pp. 80
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Wendel Cruvinel De Sousa ◽  
Carlos Frederico De Souza Castro
Keyword(s):  
Antioxidant Activity ◽  
Refractive Index ◽  
Antifungal Activity ◽  
Essential Oil ◽  
High Efficiency ◽  
Optical Rotation ◽  
Antioxidant Agent ◽  
Agricultural Use ◽  
Liquid Yield ◽  
Organoleptic Parameters

<p class="Normal1">The objective of this work was to characterize the chemical and physicochemical profile, and the antioxidant and antifungal activities of the essential oil from Cochlospermum regium flowers. The flowers were collected in a riparian Cerrado area. The essential oil was extracted by hidrodestilation in Clevenger. The organoleptic parameters were evalueted for (color, visual and aroma), for physicochemical, yield (%), solubility, optical rotation (αD ), refractive index (20 °C) and density (25 °C g mL-1), antioxidant activity were determined by the reduction of free radical DPPH, and the antifungal activity was determined through the percentage of growth inhibition on Sclerotinia sclerotiorum, Colletotrichum gloeosporioides and Aspergillus flavus. The essential oil from C. regium flowers had an oily, clear, crystalline and strongly aromatic liquid, yield of 0.099%, positive solubility, optical rotation +36.1 αD , refractive index 1.3297 20 °C, relative density of 0.933 g mL-1. The chemical profile showed the following major compounds ocimene&lt;(E)-β-&gt; with 15.87%, caryophyllene E 11.53%, γ-muurolene 20.07%, bicyclogermacrene 16.11% and, rosifoliol with 31.09%. The antioxidant activity showed high efficiency in reducing of the DPPH radical in all concentrations varied between 50 to 0.031 mg mL -1 between 95.25% to 40.09%. The antifungal activity showed 100% of mycelial inhibition in 100 and 50 µL mL-1 concentrations for S. sclerotinia, C. gloeosporioides and A. flavus. In the lowest concentration of 1.56 µL mL-1 inhibition was also observed for S. sclerotiorum and A. flavus and for 3.13 µL mL-1 for C. gloeosporioides with 26.12; 6.08 and 10.07%, respectively. The essential oil of the flowers from C. regium proved to be an efficient natural product for the development of medicines, as an antioxidant agent and, antifungal for agricultural use.</p>

scholarly journals Chemical profile, antimicrobial activity, toxicity on Artemia salina and anti-acetylcholinesterase enzyme essential oil from Bauhinia ungulata L. (Fabaceae) leaves

2016 ◽  
Vol 10 (29) ◽  
pp. 442-449 ◽  
Author(s):  
R N A Medeiros Sandra ◽  
A de Melo Filho Antonio ◽  
N R da Costa Habdel ◽  
dos Santos Silva Francisco ◽  
C dos Santos Ricardo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Artemia Salina ◽  
Chemical Profile ◽  
Acetylcholinesterase Enzyme

Chemical profile, nutraceutical and anti-phytobacterial properties of the essential oil from Dalea foliolosa (Fabaceae)

2017 ◽  
pp. 724 ◽  
Author(s):  
Nemesio Villa- Ruano ◽  
Yesenia Pacheco- Hernández ◽  
Efraín-Rubio- Rosas ◽  
José A. Zárate- Reyes ◽  
Edmundo Lozoya- Gloria ◽  
...  
Keyword(s):  
Biological Control ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Pseudomonas Syringae ◽  
Caryophyllene Oxide ◽  
Annual Plant ◽  
Dpph Radical ◽  
Chemical Profile ◽  
Tomato Dc3000 ◽  
Oxygenated Monoterpene

The present work describes the chemical composition of the leaf essential oils from the annual plant Dalea foliolosa, which was collected in three consecutive years (2014-2016). The amount of monoterpenes (69.5-84.6%) was higher to that of sesquiterpenes (10.3-22.9%) and aliphatic hydrocarbons (1-2.1%). Cryptone (22.3-30.6%) was the most abundant oxygenated monoterpene, followed by linalool (10.4-17.6%), caryophyllene oxide (4.6-15.3%), ascaridole (4.5-7.4%) and β-citronellol (3.8-5.6%). The hydrodistillated extract showed antioxidant activity against DPPH radical (IC50, 45-156 µg mL-1), strong anti-α-glucosidase activity (IC50, 14-47 µg mL-1) and inhibited the growth of Pseudomonas syringae pv. tabaci TBR2004 (MIC, 44-105 µg mL-1) and P. syringae pv. tomato DC3000 (MIC, 35-155 µg mL-1). The possible use of the essential oil as a novel additive for anti-hyperglycemic supplements and for the biological control of the analyzed Pseudomonas syringae varieties, may be contemplated.

scholarly journals Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Lethicia B. Brandão ◽  
Lizandra L. Santos ◽  
Rosany L. Martins ◽  
Alex B. L. Rodrigues ◽  
Erica de M. Rabelo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Cytotoxic Activity ◽  
Artemia Salina ◽  
Stage Of Development ◽  
Tridax Procumbens ◽  
Third Stage ◽  
Low Toxicity ◽  
Larvicidal Bioassay

The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of Tridax procumbens leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using Artemia salina, the larvicidal bioassay was performed with larvae in the third stage of development of the Aedes aegypti mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and γ-terpinene being the main ones. It presented antioxidant activity with an IC50 of 194.51 μg mL−1, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against A. salina, with an LC50 of 1238.67 μg mL−1, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC50 = 79.0 μg mL−1 in 24 hours and LC50 of 69.15 μg mL−1 in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of T. procumbens presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to A. salina.

scholarly journals Triagem fitoquímica e avaliação das atividades antioxidante e citotóxica de extratos etanólicos de Malvaviscus arboreus Cav. (MALVACEAE)

2017 ◽  
Vol 2 (1) ◽  
pp. 9-24
Author(s):  
Jhonatan Da Cruz Ribeiro ◽  
Marco Aurélio Sivero Mayworm
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Folk Medicine ◽  
Artemia Salina ◽  
Ethanol Solution ◽  
Cytotoxic Activities ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Root Extracts ◽  
Sedative Activity

Resumo: Malvaviscus arboreus pertencente à família Malvaceae, é utilizada em jardinagem, medicina popular e na culinária. Estudos anteriores demonstraram atividade moluscicida e alelopática de extrato das raízes; e atividade antitussígena, emoliente e sedativa de extrato das fl ores. Este trabalho visou avaliar as atividades antioxidante e citotóxica, e realizar triagem fi toquímica de extratos etanólicos produzidos a partir de segmentos de caule, folhas e fl ores de M. arboreus. A triagem fi toquímica avaliou a presença de fenóis totais, fl avonoides, quinonas, saponinas, alcaloides e antocianinas. A atividade antioxidante foi avaliada utilizando-se solução etanólica de DPPH (2,2-difenil-1-picrilidrazila) e os valores de absorbância foram interpretados em percentuais de atividade antioxidante (AA%). A atividade citotóxica foi avaliada através do teste de letalidade contra Artemia salina Leach. Demonstrou-seque todos os extratos apresentaram fenóis totais e fl avonoides, porém somente o extrato de fl ores apresentou antocianinas. Quanto à atividade antioxidante, o extrato etanólico de folhas mostrou-se o mais ativo nas concentrações de 100 μg/mL, 50 μg/mL e 10 μg/mL, comparado aos demais extratos. Na avaliação citotóxica, o extrato de fl ores demonstrou maior toxicidade sobre Artemia nas duas concentrações testadas (1mg/mL e 0,5 mg/mL). Conclui-se que os extratos de caule, folhas e fl ores de Malvaviscus arboreus têm potenciais antioxidante e citotóxico nas concentrações testadas.Palavras-chaves: Malvaviscus arboreus; Triagem fi toquímica; Atividade antioxidante; Atividade citotóxica.Abstract: Malvaviscus arboreus (family Malvaceae), is used in gardening, folk medicine and in cooking. Previous studies have shown molluscicidal and allelopathic activity of the root extracts, antitussive, emollient and sedative activity from extracts of fl owers. Th is study evaluated the antioxidant and cytotoxic activities and performing phytochemical screening in ethanolic extracts from segments of stem, leaves and fl owers of M. arboreus. Th e phytochemical screening aimed to evaluate the presence of total phenols, fl avonoids, quinones, saponins, alkaloids and anthocyanins. Th e antioxidant activity was evaluated using ethanol solution of DPPH (2,2-diphenyl-1-picrylhydrazyl) and absorbance values were interpreted as a percentage of antioxidant activity (AA%). Th e cytotoxic activity of the extracts was assessed using the lethality test against Artemia salina Leach. Was shown that all extracts showed total phenols and fl avonoids, but only the fl ower extract showed anthocyanins. As the antioxidant activity, the leaves extract was found to be the most active in the concentrations of 100 μg/mL, 50 μg/mL and 10 μg/mL, when compared with other extracts. In the cytotoxic evaluation , the fl ower extract showed greater toxicity on Artemia in the two concentrations tested (1mg/mL and 0,5 mg/mL). It’s concluded that the extracts of stem, leaves and fl owers of Malvaviscus arboreus have antioxidant and cytotoxic potential, at the concentrations tested.Key-words: Malvaviscus arboreus; Phytochemical screening; Antioxidant activity; Cytotoxic activity.

scholarly journals Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers

2020 ◽  
Vol 9 (8) ◽  
pp. e454985979
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Luciene Teixeira Gonçalves Romão ◽  
Eloisa Borges dos Reis ◽  
Karla da Silva Malaquias ◽  
Carlos Frederico de Souza Castro ◽  
...  
Keyword(s):  
Essential Oil ◽  
High Efficiency ◽  
Optical Rotation ◽  
Chemical Profile ◽  
Postgraduate Students ◽  
Retention Factors ◽  
Modern Technique ◽  
Physicochemical Evaluation ◽  
Tlc Analysis ◽  
Layer Chromatography

The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 °C. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. The essential oil of B. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.

scholarly journals Phytochemical characterization, antioxidant and antibacterial activity of essential oil and extracts of Tagetes patula on Staphylococcus aureus

2020 ◽  
Vol 37 (4) ◽  
pp. 347-367
Author(s):  
Virginia Monserrate López Zambrano ◽  
Alex Alberto Dueñas Rivadeneira ◽  
José Gerardo Cuenca Nevárez ◽  
Joan Manuel Rodríguez-Díaz
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Refractive Index ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Tagetes Patula

Tagetes patula is an ornamental plant species and its essential oils contain potentially allelopathic active ingredients. The objective of the present study was to evaluate the phytochemical composition of extracts, the antioxidant and antibacterial activity of the essential oil of flowering leaves of the species, on Staphylococcus aureus. For this, the extraction of the essential oil by hydrodistillation was carried out, evaluating the physical characteristics (solubility, density and refractive index) and phytochemical characteristics of the extracts through a phytochemical screening (alkaloids, flavonoids, phenols, saponins, tannins and reducing sugars). The quantificatics of phenols in the essential oil was performed by the Folin Ciocalteu method, the antioxidant activity using the DPPH and ABTS tests, the antimicrobial activity by determining the minimum inhibitory concentration. The results obtained showed for the essential oil, a density of 0,733 g.mL-1, refractive index of 1,47 and insolubility in ethanol (70 %), presence of tannins, flavonoids and phenols in the extracts. The phenolic content was 1.024 ± 0,19 mg.g-1 TAE, the antioxidant activity with DPPH was 87,6 ± 0,18 µmol.g-1TE and with ABTS 180,83 ± 0,36 µmol.g-1 TE in Trolox equivalent. The minimum inhibitory concentration was 16,67 mm against S. aureus, in response to which it is concluded that the essential oil of T. patula had antioxidant activity against radicals DPPH and ABTS, high phenolic contents and showed antibacterial activity in vitro tests against S. aureus.

scholarly journals Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

2019 ◽  
Author(s):  
Renata do Socorro Barbosa Chaves ◽  
Rosany Lopes Martins ◽  
Alex Bruno Lobato Rodrigues ◽  
Érica de Menezes Rabelo ◽  
Ana Luzia Ferreira Farias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Artemia Salina ◽  
High Cytotoxic Activity ◽  
Origanum Majorana ◽  
Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

scholarly journals EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Oom Komala ◽  
Novi Fajar Utami ◽  
Siti Mariyam Rosdiana
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Optical Rotation ◽  
Acid Number ◽  
National Standard ◽  
Plate Count ◽  
Rosa Damascena ◽  
Specific Level ◽  
Energy Material ◽  
Plate Count Method

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.

scholarly journals AKTIVITAS ANTIMIKROBA MINYAK ESENSIAL JAHE MERAH (Zingiber officinale var. Rubrum) DAN LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI PATOGEN DAN PERUSAK PANGAN

2015 ◽  
Vol 35 (01) ◽  
pp. 43 ◽  
Author(s):  
Tita Rialita ◽  
Winiati Pudji Rahayu ◽  
Lilis Nuraida ◽  
Budi Nurtama
Keyword(s):  
Antimicrobial Activity ◽  
Refractive Index ◽  
Essential Oil ◽  
Essential Oils ◽  
Optical Rotation ◽  
Acid Number ◽  
Food Spoilage ◽  
E Coli ◽  
Spoilage Bacteria ◽  
Red Ginger

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B.cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, specific gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, specific gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol,- caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm.Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values,all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry.Keywords: Antimicrobial, essential oil, red ginger, red galangal ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristikfisika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC).Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik-9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambatpertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.Kata kunci: Antimikroba, jahe merah, lengkuas merah, minyak esensial

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