scholarly journals Oxidants and antioxidants in myocardial infarction (MI); Investigation of ischemia modified albumin, malondialdehyde, superoxide dismutase and catalase in individuals diagnosed with ST elevated myocardial infarction (STEMI) and non-STEMI (NSTEMI)

2020 ◽  
Author(s):  
Nesim Aladağ ◽  
Ramazan Asoğlu ◽  
Mahmut Özdemir ◽  
Emin Asoğlu ◽  
Rukiye Derin Atabey ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Superoxide Dismutase ◽  
Healthy Subjects ◽  
Antioxidant Defense ◽  
Myocardial Damage ◽  
Significant Negative Correlation ◽  
Healthy Controls ◽  
Coronary Artery Lesions ◽  
Ischemia Modified Albumin ◽  
Coronary Ischemia

Background: Coronary ischemia can lead to myocardial damage and necrosis. The pathogenesis of cardiovascular diseases often includes increased oxidative stress and decreased antioxidant defense. The study aimed to assess levels of ischemia modified albumin (IMA), malondialdehyde acid (MDA), superoxide dismutase (SOD), and catalase in individuals diagnosed with ST elevated myocardial infarction (STEMI) and non-STEMI. Methods: The present study prospectively included 50 STEMI patients, 55 NSTEMI patients, and 55 healthy subjects. Only patients who were recently diagnosed with STEMI or NSTEMI were included in this study. IMA, MDA, SOD, and catalase activities were measured spectrophotometrically. Significant coronary artery lesions were determined by angiography. Results: Patients with ACS had significantly greater IMA and MDA values than the healthy controls (p<0.001). In addition, patients with STEMI had IMA levels that were significantly greater than those of the patients with NSTEMI (p <0.001), while the reverse was true for MDA levels (p<0.001). The healthy controls had the highest levels of SOD and catalase levels, followed by patients with STEMI and patients with NSTEMI, respectively (p <0.001). There was a significant negative correlation among MDA and SOD with catalase levels (r = -0.771 p <0.001 MDA vs catalase; r = -0.821 p <0.001 SOD vs catalase). Conclusions: Data obtained in this study reveals that compared to healthy controls, STEMI and NSTEMI patients had increased levels MDA and IMA, and decreased levels of SOD and catalase.

Evaluation Of Antioxidant Defense In Patients With Colorectal Carcinoma

2015 ◽  
Vol 87 (7) ◽  
Author(s):  
Katarzyna Malinowska ◽  
Michał Mik ◽  
Łukasz Dziki ◽  
Adam Dziki ◽  
Ireneusz Majsterek
Keyword(s):  
Colorectal Cancer ◽  
Superoxide Dismutase ◽  
Colorectal Carcinoma ◽  
Glutathione Peroxidase ◽  
Healthy Subjects ◽  
Antioxidant Defense ◽  
Antioxidant Response ◽  
Antioxidant Enzyme Activity ◽  
Control Group ◽  
Healthy Controls

AbstractCancers are among the most feared diseases of modern civilization. In Poland, colorectal cancer is one of the tumors with the worst prognosis. The ability to cure is primarily dependent on the stage of the disease at the time of diagnosis.was evaluate antioxidant response in patients with colorectal carcinoma.Twenty patients (14 men and 6 women) aged 61.9± 11.1 years with colorectal cancer were included in the study. Twenty healthy subjects (4 men and 16 women) aged 64 ± 15.3 years formed the control group. The erythrocyte activities of antioxidant enzymes, superoxide dismutase (SOD), and glutathione peroxidase (GPx),A significant increase of GPx, and SOD (p < 0.05) were seen in patients compared to healthy controls.The results indicate that the tested antioxidant enzyme activity of glutathione peroxidase and superoxide dismutase is increased in patients diagnosed with colorectal cancer compared to the control group.

Platelets from Patients with Myocardial Infarction Can Activate T Cells in Vitro

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 3448-3448 ◽  
Author(s):  
Elena E. Solomou ◽  
Vasilios Gizas ◽  
Theodora Babali ◽  
Angelos Perperis ◽  
Evgenia Verigou ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
T Cells ◽  
T Cell ◽  
Unstable Angina ◽  
T Cell Activation ◽  
Healthy Subjects ◽  
Cell Activation ◽  
Control Group ◽  
Healthy Controls

Abstract Specific aim: Our aim was to examine whether T cells can be activated by the circulating activated platelets from patients with myocardial infarction (with ST elevation-STEMI) Methods: After written informed consent was obtained, peripheral blood mononuclear cells (PBMCs) were isolated from heparinized venous blood obtained from 20 patients with STEMI (18 men and 2 women) at the time of hospital admission at diagnosis, before receiving any treatment, as well as 5 days and 30 days later. We also analyzed 10 healthy subjects (8 men and 2 women), and 5 patients with unstable angina who served as the disease control group. PBMCs were analyzed by flow cytometry with the following markers and their isotypic controls: CD4, CD25, CD69, and FOXP3. We also isolated platelet rich plasma or plasma alone from the patients and the healthy subjects, and used in mixed cultures with PBMCs. Results: We first examined T cell activation by measuring CD69 expression on CD4 T cells following incubation with platelets obtained from patients with STEMI. T cells treated with platelets from patients with STEMI showed increased expression of CD69 (as an activation marker) compared with T cells treated with platelets from healthy subjects (p<0.05, Figure 1). There was no T cell activation following incubation with plasma alone from patients or healthy controls. We then examined the percentages of CD4+CD25+hi (regulatory T cells). There was no statistical difference in Tregs between patients at presentation and controls (healthy subjects and disease control group). Five days later, patients with STEMI displayed increased levels of Tregs compared with the 2 control groups; one month later, Treg numbers returned to the initial presentation levels (p<0.05, Figure 2) Conclusion: To our knowledge we describe for the first time that platelets from patients with STEMI can activate T cells in vitro. In patients with STEMI, an increase in Tregs possibly in an effort to suppress immune system activation secondary to platelet activation, appears shortly after the infarct and normalizes a month later. Figure 1. T cell activation after treatment with platelets from patients with STEMI compared with the platelets from healthy controls or plasma alone Figure 1. T cell activation after treatment with platelets from patients with STEMI compared with the platelets from healthy controls or plasma alone Figure 2. Increased T regs in patients with STEMI , 5 days after admission (STEMI EXIT) ( UA;unstable angina, STEMI 1mfup; STEMI after one month) Figure 2. Increased T regs in patients with STEMI , 5 days after admission (STEMI EXIT) ( UA;unstable angina, STEMI 1mfup; STEMI after one month) Disclosures No relevant conflicts of interest to declare.

Butyrylcholinesterase as an additional marker in the diagnostic network of acute myocardial infarction

2016 ◽  
Vol 40 (2) ◽  
Author(s):  
Ramazan Kocabaş ◽  
Ali Kemal Erenler ◽  
Mücahit Yetim ◽  
Tolga Doğan ◽  
Hacı Kemal Erdemli
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Healthy Subjects ◽  
Early Stage ◽  
Characteristic Curve ◽  
Myocardial Damage ◽  
Diagnostic Value ◽  
Coronary Syndrome ◽  
Additional Marker ◽  
Low Sensitivity

AbstractAcute coronary syndrome defines a broad spectrum of complaints from angina to irreversible myocardial damage. There is an ongoing need for a biomarker to predict and diagnose acute myocardial infarction (AMI) in the early stage. In this study, our aim was to reveal early diagnostic value of butyrylcholinesterase (BChE) in discrimination of healthy subjects and patients with AMI.Eighty-five patients admitted to our hospital due to AMI and 45 healthy subjects were involved in the study. Patients and controls were compared according to BChE, lipid profiles and biochemical parameters.The serum BChE activity was significantly lower in patients with AMI than in the controls (p<0.001). After correlation analysis, while a negative correlation was determined between the serum BChE concentrations and AMI presence (r=–0.363, p<0.001); a positive correlation was determined between the serum BChE and cholesterol (r=0.443, p<0.001), HDL (r=0.243, p=0.006) and LDL (r=0.369, p<0.001) levels. The data indicate that BChE is associated with AMI and a subsequent receiver operating characteristic curve (ROC) analysis revealed that BChE, as an independent indicator, may differentiate AMI patients from controls. A cut-off point set at ≤7.15 kIU/L, BChE showed a sensitivity of 51.2% and a specificity of 84.4% (AUC=0.719, p<0.001).Low BChE level was significantly associated with AMI when compared to healthy subjects. Even though it has low sensitivity, plasma levels of BChE might represent an additional marker in the diagnostic network of AMI.

scholarly journals Analysis of prototypical narratives produced by aphasic individuals and cognitively healthy subjects

2015 ◽  
Vol 9 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Gabriela Silveira ◽  
Letícia Lessa Mansur
Keyword(s):  
Fairy Tales ◽  
Healthy Subjects ◽  
Language Assessment ◽  
Significant Negative Correlation ◽  
Brazilian Portuguese ◽  
Written Language ◽  
Healthy Controls ◽  
Language Discourse ◽  
Normal Individuals ◽  
Significant Difference

Aphasia can globally or selectively affect comprehension and production of verbal and written language. Discourse analysis can aid language assessment and diagnosis. Objective: [1] To explore narratives that produce a number of valid indicators for diagnosing aphasia in speakers of Brazilian Portuguese. [2] To analyze the macrostructural aspects of the discourse of normal individuals. [3] To analyze the macrostructural aspects of the discourse of aphasic individuals. Methods: The macrostructural aspects of three narratives produced by aphasic individuals and cognitively healthy subjects were analyzed. Results: A total of 30 volunteers were examined comprising 10 aphasic individuals (AG) and 20 healthy controls (CG). The CG included 5 males. The CG had a mean age of 38.9 years (SD=15.61) and mean schooling of 13 years (SD=2.67) whereas the AG had a mean age of 51.7 years (SD=17.3) and mean schooling of 9.1 years (SD=3.69). Participants were asked to narrate three fairy tales as a basis for analyzing the macrostructure of discourse. Comparison of the three narratives revealed no statistically significant difference in number of propositions produced by the groups. A significant negative correlation was found between age and number of propositions produced. Also, statistically significant differences were observed in the number of propositions produced by the individuals in the CG and the AG for the three tales. Conclusion: It was concluded that the three tales are applicable for discourse assessment, containing a similar number of propositions and differentiating aphasic individuals and cognitively healthy subjects based on analysis of the macrostructure of discourse.

The association between healthy eating index and serum antioxidant indices in patients with different degrees of cataract compared to healthy subjects: a case-control study

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Naeimeh Heidari ◽  
Masoumeh Jabbari ◽  
Mina Babashahi ◽  
Reza Nabie ◽  
Mohammad Asghari Jafarabadi ◽  
...  
Keyword(s):  
Healthy Eating ◽  
Healthy Subjects ◽  
Case Control Study ◽  
Significant Negative Correlation ◽  
Case Control ◽  
Healthy Eating Index ◽  
Healthy Controls ◽  
Content Type ◽  
Control Study ◽  
Cataract Patients

Purpose Studies conducted on association between diet and cataract, found conflicting results. This paper aims to investigate the association between healthy eating index (HEI) and serum antioxidant and oxidant indices in patients with different degrees of cataract compared to the healthy subjects. Design/methodology/approach Ninety volunteers (aged > 50 years) were divided into the cataract (n = 45) and healthy control (n = 45) groups in this case-control study. Anthropometric variables, HEI score, serum total oxidant capacity (TOC) and total antioxidant capacity (TAC), blood pressure, physical activity and stress measurements were done for all participants. Findings There was a significant difference in stress level between subgroup of first-degree cataract and healthy controls (16.6 ± 2.3 vs 42.5 ± 29.0, p < 0.001) as well as third-degree cataract (16.6 ± 2.3 vs 75.2 ± 22.6, p < 0.001). Serum TAC was significantly higher in healthy people compared to the first-degree (1.2 ± 0.2 vs 1 ± 0.2, p = 0.002) and third-degree cataract patients (1.2 ± 0.2 vs 1.0 ± 0.2, p = 0.013). Also, serum TOC was significantly lower in healthy controls compared to the first-degree (8.2 ± 1.1 vs 9.2 ± 1.0, p = 0.006) and third-degree cataract patients (8.2 ± 1.1 vs 9.1 ± 1.2, p = 0.015). There was a significant direct correlation between moderation (r = 0.61, p = 0.019) and total score of HEI (r = 0.57, p = 0.031) with serum TAC only in the first-degree cataract patients. Also there was a significant negative correlation between moderation score of HEI with serum TOC in the first-degree cataract patients (r = −0.60, p = 0.025), and there was a significant negative correlation between moderation (r = −0.36, p = 0.017) and total score (r=−0.35, p = 0.021) of HEI and TOC in the total cataract patients. Originality/value To the best of the authors’ knowledge, this is the first study on the assessment of the relationship between components of HEI and serum antioxidant indicators in different types of cataract compared to healthy individuals. It could be a start point for more detailed and large-scaled studies, even intervention studies, in this field.

Relationship Between Fibrinopeptide A and Fibrinogen/Fibrin Fragment E in Thromboembolism, DIC and Various Non-Thromboembolic Diseases

1987 ◽  
Vol 58 (02) ◽  
pp. 758-763 ◽  
Author(s):  
G Mombelli ◽  
R Monotti ◽  
A Haeberli ◽  
P W Straub
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Liver Cirrhosis ◽  
Healthy Subjects ◽  
Vascular Diseases ◽  
Fibrinopeptide A ◽  
Normal Range ◽  
Collagen Vascular Diseases ◽  
Intravascular Coagulation ◽  
Fibrin Formation

SummaryIncreased fibrinopeptide A (FPA) levels have been reported in various non-thrombotic disorders, including cancer, acute myocardial infarction, liver cirrhosis and collagen vascular diseases. To investigate the significance of these findings, the present study combined the radioimmunoassay of FPA with that of fibrinogen/fibrin degradation fragment E (FgE) in the aforementioned disorders and compared the results with those observed in healthy subjects as well as in patients with thromboembolism and overt disseminated intravascular coagulation (DIC). Mean FPA and FgE in malignancy were 6.3 and 305 ng/ml, in myocardial infarction 5.6 and 98 ng/ml, in liver cirrhosis 2.7 and 132 ng/ml and in collagen vascular diseases 5.6 and 142 ng/ml. All these values were significantly higher than in healthy controls (mean FPA 1.6 ng/ml, mean FgE 49 ng/ml) but significantly lower than in thromboembolism (mean FPA 10.7 ng/ml, mean FgE 639 ng/ ml) and DIC (mean FPA 22.0 ng/ml, mean FgE 1041 ng/ml). The overall correlation between FPA and FgE was highly significant. Elowever, different disorders showed peculiar patterns in FPA, FgE and fibrinogen levels. In malignancy, a definite increase of FPA, FgE and plasma fibrinogen levels was observed. This finding probably indicates a compensated state of (intra- or extravascular) fibrin formation and lysis. Acute myocardial infarction was characterized by a high FPA to FgE ratio, which is interpreted to reflect acute thrombin generation and fibrin formation. FPA in cirrhosis was only marginally elevated with most single values within the normal range, indicating that intravascular coagulation was infrequent and unimportant in quantitative terms.

Plasma D Dimer: A Useful Marker of Fibrin Breakdown in Renal Failure

1989 ◽  
Vol 61 (03) ◽  
pp. 522-525 ◽  
Author(s):  
M P Gordge ◽  
R W Faint ◽  
P B Rylance ◽  
H Ireland ◽  
D A Lane ◽  
...  
Keyword(s):  
Renal Failure ◽  
Renal Function ◽  
Acute Renal Failure ◽  
Monoclonal Antibodies ◽  
Diabetic Nephropathy ◽  
Renal Disease ◽  
Plasma Concentrations ◽  
Significant Negative Correlation ◽  
Healthy Controls ◽  
D Dimer

SummaryD dimer and other large fragments produced during the breakdown of crosslinked fibrin may be measured by enzyme immunoassay using monoclonal antibodies. In 91 patients with renal disease and varying degrees of renal dysfunction, plasma D dimer showed no correlation with renal function, whereas FgE antigen, a fibrinogen derivative which is known to be cleared in part by the kidney, showed a significant negative correlation with creatinine clearance. Plasma concentrations of D dimer were, however, increased in patients with chronic renal failure (244 ± 3l ng/ml) (mean ± SEM) and diabetic nephropathy (308 ± 74 ng/ml), when compared with healthy controls (96 ± 13 ng/ml), and grossly elevated in patients with acute renal failure (2,451 ± 1,007 ng/ml). The results indicate an increase in fibrin formation and lysis, and not simply reduced elimination of D dimer by the kidneys, and are further evidence of activated coagulation in renal disease. D dimer appears to be a useful marker of fibrin breakdown in renal failure.

A Common Thrombomodulin Amino Acid Dimorphism Is Associated with Myocardial Infarction

1997 ◽  
Vol 77 (02) ◽  
pp. 248-251 ◽  
Author(s):  
Lena Norlund ◽  
Johan Holm ◽  
Bengt Zöller ◽  
Ann-Kristin Öhlin
Keyword(s):  
Myocardial Infarction ◽  
Amino Acid ◽  
Plasma Concentration ◽  
Acute Coronary Syndromes ◽  
Protein C ◽  
Integral Membrane Protein ◽  
Control Group ◽  
Healthy Controls ◽  
Coronary Syndromes ◽  
Premature Myocardial Infarction

SummaryEndothelial dysfunction and haemostatic imbalance are believed to be important aetiological factors in the development of acute coronary syndromes. Thrombomodulin (TM) is an integral membrane protein crucial for normal endothelial function and activation of the protein C anticoagulant pathway. We have investigated the importance of a common C/T dimorphism in the TM gene (nucleotide 1418) for development of premature myocardial infarction (MI). The C/T dimorphism predicts an Ala455 to Val replacement in the sixth EGF-like domain of TM. The dimorphism was investigated in 97 MI survivors and 159 healthy controls. The C allele was significantly more frequent among patients than controls (p = 0.035). The allele frequency for the C allele was 0.82 in the patients and 0.72 in the control group. The plasma concentration of TM was investigated among healthy controls but was not related to the C/T dimorphism. In conclusion, the association of the C allele with premature MI, suggests that the TM gene and the C/T dimorphism may be aetiological factors involved in the pathogenesis of MI. Possibly, the Ala455 to Val replacement may affect the function of the TM molecule and the activation of the protein C anticoagulant pathway.

STUDY OF THE COMBINED INFLUENCE OF SODIUM THIOPENTAL AND DELTA SLEEP-INDUCING PEPTIDE ON ANTIOXIDANT DEFENSE SYSTEM IN RATS

2016 ◽  
pp. 49-52
Author(s):  
A. V. Shvetsov ◽  
E. G. Batotsyrenova ◽  
N. A. Dyuzhikova ◽  
V. A. Kashuro ◽  
N. V. Lapina ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Time Interval ◽  
Sodium Thiopental ◽  
Antioxidant Defense Enzymes ◽  
Delta Sleep ◽  
Thiopental Coma ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Delta Sleep Inducing Peptide

A biochemical investigation was performed into activity of rat antioxidant defense enzymes at different time interval after administration of sodium thiopental and delta-sleep-inducing peptide (DSIP). It was shown that thiopental coma was accompanied by a decreased level of superoxide dismutase ( 6 and 24 h after exposure) and increased level of caspase-3 ( 6 h after exposure) in the rat blood plasma. A pharmacological correction with DSIP induced a decrease of the level of superoxide dismutase ( 6 and 24 h after exposure), glutathione peroxidase and glucose 6-phosphate dehydrogenase (after 6h).

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