scholarly journals Complex analysis of total protein content in bread wheat containing GPC-B1 gene from Triticum turgidum SSP. dicoccoides

2017 ◽  
Vol 15 (1) ◽  
pp. 52-57 ◽  
Author(s):  
S. Yu. Pokhylko ◽  
S. V. Schwartau ◽  
V. V. Pochynok ◽  
L. M. Mykhalska ◽  
O. M. Dugan ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Complex Analysis ◽  
Triticum Turgidum ◽  
Infrared Spectrometry ◽  
Total Protein Content ◽  
Genetic Environment ◽  
B1 Gene ◽  
Kjeldahl Method ◽  
Hybrid Lines

Aim. The purpose of our study was to determine the content of total protein in the F5 generation grains, the carriers of the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides by two methods, which in turn would confirm the successful expression of the Gpc-B1 gene in the genetic environment of bread winter wheat. Methods. Determination of protein content was carried out by Kjeldahl method and by infrared spectrometry (NIR) method. Results. The 44 hybrid lines that are homozygous for the Gpc-B1 gene from T. turgidum ssp. dicoccoides have been analyzed. It has been established that for both methods, the average content of protein in the grain of hybrid lines is 14 % higher in comparison to the original Kuyalnik variety. Particular attention should be paid to the line number 10, 12 and 35 in which the content of protein exceeds 15 % by the method of Kjeldahl. Conclusions. The obtained results indicate that the gene Gpc-B1 from the wild relative in the new genetic environment of the highly productive registered wheat cultivar Kuyalnik has been functioning and has a positive effect on the accumulation of total protein in grains.Keywords: biofortification, protein content, Triticum aestivum, Gpc-B1 gene, Kjeldahl and NIR methods.

scholarly journals Analysis of Fatty acid and Determination of Total Protein, Alkaloid, Saponin, Flavonoid of Bangladeshi Bombax ceiba Linn Leaves and Seeds

2016 ◽  
Vol 4 (04) ◽  
pp. 23-28
Author(s):  
Shahin Aziz ◽  
Shahal Ahmed ◽  
Sharmin Akter Lisa ◽  
Tanzima Parvin
Keyword(s):  
Fatty Acid ◽  
Protein Content ◽  
Total Protein ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Fatty Acid Analysis ◽  
Total Protein Content ◽  
Saponin Content ◽  
Kjeldahl Method

Bombax ceiba Linn belongs to the family of Bombacaceae and is an important medicinal plant. In Bangladesh, Bombax ceiba Linn is locally known as “Shimul tree”. The whole part of the plant used as traditional folk medicine for the treatment of antidysentric, anti diahorreal and antipyretic effects. The present communication attempts to evaluate fatty acid analysis by GC-MS spectrophotometer, total protein content by Kjeldahl method and to quantify some active constituents i.e. alkaloid, saponin and flavonoid. The fatty acid compositions of the petroleum ether extract of leaves and seeds of Bombax ceiba grown in Bangaladesh were determined by gas chromatography- mass spectrophotometer. 8 compounds were identified from leaves and 13 compounds were identified from the seeds. For both cases Palmitic acid showed higher value. The findings from present study showed the protein content for seeds have higher value (18.89%) than leaves of Bombax ceiba . The present investigation showed that both leaves and seeds of Bombax ceiba contain phytochemicals such as flavanoids, alakaloids and saponins in appreciable quantities. The flavonoid content of leaves was 5.97% and for the case of seeds (5.72%), the alkaloid content for leaves was (9.73%) and for seeds (31.44), the saponin content for the case of leaves (13.90%) and for the case of seeds was(43.58%).

scholarly journals Blue lupine seeds protein content and amino acids composition

2018 ◽  
Vol 64 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
Tomczak Aneta ◽  
Zielińska-Dawidziak Magdalena ◽  
Piasecka-Kwiatkowska Dorota ◽  
Lart-Szczapa Eleonora
Keyword(s):  
Amino Acids ◽  
Protein Content ◽  
Total Protein ◽  
Ultra Performance Liquid Chromatography ◽  
Point Of View ◽  
Total Protein Content ◽  
Amino Acids Composition ◽  
Kjeldahl Method ◽  
The One ◽  
Two Parameters

Lupine seeds are promising soy replacement in food products and feeds. Eighteen cultivars of blue lupine seeds were examined to indicate the one most favourable in protein content and quality. Two parameters were studied, i.e. total protein content (with the Kjeldahl method) and amino acids composition (with the ultra performance liquid chromatography technique). Both parameters studied were variable and depended both on the cultivar and on the place of cultivation. Protein content was in the range of 28–41% and the worst cultivar, regardless of cultivation place, was cv. Kalif (average total protein content 29.37 ± 1.14%), while the best cultivar was Boruta (average total protein content 37.43 ± 0.98%). The blue lupine seeds were rich especially in leucine (5.3 ± 0.5–9.7 ± 0.5 g/16 g N), threonine (2.4 ± 0.7–4.9 ± 0.1 g/16 g N) and lysine (2.7 ± 0.4–5.6 ± 0.1 g/16 g N). The richest among all amino acids studied were the cv. Bojar seeds from Wiatrowo and cv. Oskar from Przebędowo. It was not possible to choose one cultivar preferable from the nutritional point of view, because a strong influence of cultivation place on protein content and quality was observed, especially as to the precipitation sum.

scholarly journals Nitrogen and protein contents in some aquatic plant species

2015 ◽  
Vol 46 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Krystyna Bytniewska
Keyword(s):  
Protein Content ◽  
Plant Species ◽  
Aquatic Plants ◽  
Total Protein ◽  
Dry Matter ◽  
Aquatic Plant ◽  
Natural Habitat ◽  
Total Protein Content ◽  
Nitrogen Compounds ◽  
Kjeldahl Method

Nitrogen and protein contents in higher aquatic plants deriving from a natural habitat were determined. The following plants were examined: <i>Spirodela polyrrhiza</i> (L.) Schleid., <i>Elodea canadensis</i> Rich., <i>Riccia fluitans</i> L. Total nitrogen and nitrogen of respective fractions were determined by the Kjeldahl method. Nitrogen compounds were fractionated according to Thimann et al. Protein was extracted after Fletcher and Osborne and fractionated after Osborne. It was found, that total protein content in the plants under examination constitutes 18 to 25%o of dry matter. Albumins and glutelins are the most abundant protein fractions.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

Development and regression of exercise-induced cardiac hypertrophy in rats

1979 ◽  
Vol 236 (2) ◽  
pp. H268-H272 ◽  
Author(s):  
R. C. Hickson ◽  
G. T. Hammons ◽  
J. O. Holoszy
Keyword(s):  
Cytochrome C ◽  
Protein Content ◽  
Total Protein ◽  
Female Rats ◽  
Heart Weight ◽  
Total Protein Content ◽  
Mitochondrial Marker ◽  
Time Intervals ◽  
Control Value ◽  
Exercise Induced

Adult female rats were exercised by daily swimming. All the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. The half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. The total amounts of DNA and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10%, respectively). Other animals were subjected to the same swimming program for 21 days. Groups of rats were killed at various time intervals after stopping exercise. Heart weight, total protein content, and total cytochrome c content decreased rapidly initially, with 60% of the total regression of hypertrophy occurring during the first week. Thereafter, heart weight fell more gradually toward the sedentary control value. The hydroxyproline content of the heart, which was increased 10%, did not decrease during the regression of the hypertrophy.

scholarly journals Amino Acid Composition of Milk from Cow, Sheep and Goat Raised in Ailano and Valle Agricola, Two Localities of ‘Alto Casertano’ (Campania Region)

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2431
Author(s):  
Nicola Landi ◽  
Sara Ragucci ◽  
Antimo Di Maro
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Content ◽  
Total Protein ◽  
Free Amino ◽  
Raw Milk ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Campania Region

Cow, sheep and goat raw milk raised in Ailano and Valle Agricola territories (‘Alto Casertano’, Italy) were characterized (raw proteins, free and total amino acids content) to assess milk quality. Raw milk with the highest total protein content is sheep milk followed by goat and cow milk from both localities. Total amino acid content in cow, goat and sheep raw milk is 4.58, 4.81 and 6.62 g per 100 g, respectively, in which the most abundant amino acid is glutamic acid (~20.36 g per 100 g of proteins). Vice versa, the free amino acids content characteristic profiles are different for each species. In particular, the most abundant free amino acid in cow, sheep and goat raw milk is glutamic acid (9.07 mg per 100 g), tyrosine (4.72 mg per 100 g) and glycine (4.54 mg per 100 g), respectively. In addition, goat raw milk is a source of taurine (14.92 mg per 100 g), retrieved in low amount in cow (1.38 mg per 100 g) and sheep (2.10 mg per 100 g) raw milk. Overall, raw milk from ‘Alto Casertano’ show a high total protein content and are a good source of essential amino acids.

STUDYING THE INFLUENCE OF SEASONAL FACTORS ON CASEIN AND WHEY PROTEIN CONTENT IN RAW MILK AND CHEESE YIELD

2020 ◽  
pp. 142-151
Author(s):  
L. Bahdanava ◽  
A. Podryabinkina ◽  
I. Bahdanau ◽  
T. Savelyeva
Keyword(s):  
Protein Content ◽  
Whey Protein ◽  
Total Protein ◽  
Seasonal Changes ◽  
Whey Proteins ◽  
Raw Milk ◽  
National Average ◽  
Total Protein Content ◽  
Seasonal Factors

The article presents the results of research to study seasonal changes in the content of total protein, casein and whey proteins in raw milk and to analyze their impact on cheese yield. It was determined that the lowest casein content in raw milk (18% lower than the national average) was observed in October and March. The linear dependence of the cheese yield on both the total protein content and casein content was established.

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