scholarly journals Effectiveness of Cordyceps sp. and Beauveria Bassiana Against Large Cabbage-heart Caterpillar, Crocidolomia Pavonana Fabricius (Lepidoptera: Crambidae)

2022 ◽  
Vol 16 ◽  
pp. 14-18
Author(s):  
Agus Dana Permana ◽  
Sally Marlinda ◽  
Agus Susanto ◽  
Amin Setyo Leksono
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insect Pest ◽  
Probit Analysis ◽  
Efficacy Trial ◽  
Natural Area ◽  
Cooking Oil ◽  
Field Efficacy ◽  
Major Pest ◽  
Screen House

Crocidolomia pavonana Fabr. (Lepidoptera: Crambidae) is a major pest on various cabbage crops in Indonesia. Various insecticides have been used to control this insect pest including several entomopathogenic fungi, and this cabbage caterpillar has become resistant to various insecticides. In order to develop insecticides from entomopathogenic fungi, species or varieties from natural area such as forest are needed. A bioassays of Cordyceps sp. and Beauveria bassiana has been carried out in the laboratory. Inoculum of Cordyceps sp. obtained from the Dipterocarpaceae forest in South Kalimantan, Indonesia recovered from dead ants by the mycelium of Cordyceps sp. Four concentration of Cordyceps sp. and Beauveria bassiana inoculum and tween80 as a control were tested against 3rd instar larvae of C. pavonana with five replications. Field efficacy trial was carried out in a screen house (8 x 12 M2) using Chinese cabbage plants (Brassica juncea) aged 28 days after sowing, with a spacing of 0.4 M between rows. Three formulation of Cordyceps sp. and tween80 as a control were used with 6 replications. The result of the bioassays in the laboratory showed that Cordyceps sp. with concentration of 1 x 107 conidia mL-1 was able to give 100% mortality od C. pavonana larvae, while B. bassiana with the same concentration caused morality of C. pavonana larvae of 98.33%, although statistically not significantly different. The results of probit analysis also showed that LC90 Cordyceps sp. against cabbage caterpillar larvae was 1.57 x 106 conidia mL-1, while B. bassiana required a concentration of 2.6 x 106 conidia mL-1 to reach the LC90 value. The results of filed trial showed that the formulation of Cordyceps sp. 1 x 107 conidia mL-1 using palm oil cooking oil was able to give an average mortality of 86.7%, and significantly higher (P<0.05) than the other treatment. The results of this study indicate that the inoculum of Cordyceps sp. from Dipterocarpaceae forest is very promising to be developed as an insecticide to control C. pavonana in cabbage

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

scholarly journals JAMUR ENTOMOPATOGEN ASAL TANAH LEBAK DI SUMATERA SELATAN DAN POTENSINYA SEBAGAI AGENSIA HAYATI WALANG SANGIT (LEPTOCORISA ORATORIUS (F.))

2010 ◽  
Vol 10 (2) ◽  
pp. 154-161
Author(s):  
Effendy TA ◽  
Robby Septiadi ◽  
Abdullah Salim ◽  
Abdul Mazid
Keyword(s):  
Beauveria Bassiana ◽  
Analysis Of Variance ◽  
Entomopathogenic Fungi ◽  
Probit Analysis ◽  
Biological Agents ◽  
Biocontrol Agents ◽  
Stink Bug ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Leptocorisa Oratorius

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F).  Soil used for trapping entomopatogenic fungi, was taken from five locations  in South Sumatera.  Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design.  LT50­ of nymph mortality was determined with Probit Analysis.  Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp.  Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates.  The viability of B. bassiana conidia did not decrease during sub-culturing.  Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50­  of the stink bug.

scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

Virulence of Some Entomopathogenic Fungi Isolates of Beauveria bassiana (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Hypocreales: Clavicipitaceae) to Aulacaspis tubercularis (Hemiptera: Diaspididae)and Icerya seychellarum (Hemiptera: Monophlebidae) on Mango Crop

2019 ◽  
Author(s):  
Atef M M Sayed ◽  
Christopher A Dunlap
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
Probit Analysis ◽  
Scale Insects ◽  
Elapsed Time ◽  
Fungal Isolates ◽  
Further Development

Abstract Six fungal isolates of Beauveria bassiana (Balsamo) Vuillemin and one isolate of Metarhizium anisopliae (Metschnikoff) Sorokin were isolated and evaluated for their pathogenicity to Icerya seychellarum (Westwood) and Aulacaspis tubercularis Newstead. There is a positive correlation between the concentration of the fungal blastospore concentrations and the percentage of mortality. Bio-efficacy increased significantly after inoculation with increasing concentration of blastospores and elapsed time up to 12 d after inoculation. The mortality of nymphs exposed to fungal isolates at various concentrations varied between 2.5 and 88.8%. Probit analysis of data at 95% confidence limits of LC50 and LT50s showed significant differences in the susceptibility of nymphs of I. seychellarum and A. tubercularis to the tested fungal isolates. The fungal isolates of Egy-6 and Egy-9 were the most effective against I. seychellarum and A. tubercularis, respectively. They had the lowest LC50 (4.20 × 105 and 5.71 × 103 blastospore ml−1) and LT50 (ranged from 4.61 to 9.79 and 4.84 to 8.71 d), respectively. The current study showed that all the fungal isolates yielded moderate mortality rates of nymphs and adult female populations of both the tested insect pests. To our knowledge, this is the first report of bio-efficacy of Beauveria and Metarhizium isolates against members of the Diaspidadae and Monophlebidae family insects. These results establish that the use of these native entomopathogenic fungi isolates of B. bassiana (Egy-3, Egy-4, Egy-6, Egy-7, Egy-9, and Egy-10) and M. anisopliae (Egy-5) could be considered for further development as microbial control agents of the mealybug and scale insects as a potential biological agent for use in an IPM program.

scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

scholarly journals Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama

2020 ◽  
Vol 1 (2) ◽  
pp. 70
Author(s):  
Arsi Arsi ◽  
Yulia Pujiastuti ◽  
Suparman Surya Hadi Kusuma ◽  
Bambang Gunawan
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Insect Pest ◽  
Entire Body ◽  
Vegetable Crops

ABSTRACT Entomopathogenic fungi are classified as a fungus that infects insect pests. Hyphae color that comes out of the insect's body depends on the type of entomopathogenic fungus that attacks it. The purpose of this research is to explore, isolate and identify entomopathogenic fungi that attack insect pests. Samples of dead insects were taken from vegetable crops in the highlands of Pagaralam City and the lowlands of Ogan Komering Ilir Regency. In addition, exploration is also carried out using insect bait methods. This method is carried out on soils taken from the highlands of the fencealam area and Pekanbaru area. Based on the results of entomopathogenic expolation in vegetable plants that infect insects in the field there are 2 types of fungi that attack the insect. Two types of entomapatogenic fungi were found to have characteristics, namely the first hyphal color is rather green which covers the entire body of the insect and the second is white hyphae. The fungus that attacks the insect, then isolated to the GYA media found two entomopathogenic fungi namely, Metarhizium sp. and Beauveria bassiana. Meanwhile, through insect bait using soil T. molitor larvae, one species of entomopathogenic fungus was found, namely Metarhizium sp. The most infected T. molitor larvae in the soil of origin of Pekanbaru in the 2nd and 4th weeks, namely, 21.90 and 29.33 tails.   . Keywords: Entomopathogenic Fungus, Insect Pest and Tenibrio molitor  

Evaluation of entomopathogenic fungi against Raoiella indica Hirst (Acari: Prostigmata: Tenuipalpidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 219-224
Author(s):  
Indhusri Chavan ◽  
S. Pradeep ◽  
S. Sridhara ◽  
K.N. Ashrith
Keyword(s):  
Laboratory Condition ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Fungal Pathogens ◽  
Environmentally Friendly ◽  
Probit Analysis ◽  
Life Stages ◽  
Leaf Discs ◽  
Lecanicillium Lecanii ◽  
Raoiella Indica

Entomopathogenic fungi Metarrhizium anisopliae, Beauveria bassiana and Lecanicillium lecanii tested against immature and adults of Raoiella indica under laboratory condition with five different concentrations of each sprayed on leaf discs containing larvae, nymphs, and adults, indicated that all life stages were susceptible. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, L. lecanii was the most virulent with LC50 of 8.15 × 105 conidia ml-1 and 1.30 × 105 conidia ml-1 followed by M. anisopliae 18.05 x 105 conidia ml-1 and 2.70 × 105 conidia/ml and B. bassiana (27.13 × 105 conidia ml-1 and 4.80 × 105 conidia ml-1) for immature and adults, respectively. However the efficacy of the fungal pathogens evaluated clearly differs from that of the controls. These entomopathogenic fungi could be considered as an environmentally friendly alternative for biocontrol of R. indica.

scholarly journals Cuticle degrading enzyme production by some isolates of the entomopathogenic fungus, Metarhizium anisopliae (Metsch.)

2014 ◽  
Vol 20 ◽  
pp. 25-32
Author(s):  
N Sapna Bai ◽  
OK Remadevi ◽  
TO Sasidharan ◽  
M Balachander ◽  
Priyadarsanan Dharmarajan
Keyword(s):  
Pest Control ◽  
Entomopathogenic Fungi ◽  
Enzyme Production ◽  
Insect Pest ◽  
Strong Relationship ◽  
Control Agent ◽  
Mechanical Pressure ◽  
Degrading Enzyme ◽  
Insect Pest Control ◽  
Degrading Enzymes

Context: Entomopathogenic fungi have been recognized as viable alternate options to chemicals in insect pest control. Unlike other potential biocontrol agents, fungi do not have to be ingested to infect their hosts but invade directly through the cuticle. Entry into the host involves both enzymic degradation of the cuticle barrier and mechanical pressure. Production of a range of cuticle degrading enzymes is an important event in the interaction of entomopathogenic fungi and host. Enzyme secretion is believed to be a key contributor for the virulence of a fungal isolate. Objectives: The potentiality of nine isolates of M. anisopliae were tested to produce to produce three important cuticle degrading enzymes, viz., chitinase, protease and lipase. Materials and Methods: Nine isolates of M. anisopliae were evaluated for chitinase, protease and lipase enzyme production by determining the enzyme index and activities. Results: Chitinase index of these isolates were ranged from 1.5 to 2.2 and chitinolytic activity from 0.525 to 1.560 U/ml. The isolates showed protease index in the range of 1.2 to 3.3 and the activity ranged from 0.020 to 0.114 U/ml. Lipase index ranged from 1.15 to 7.0 and the enzyme activity ranged from 0.153 to 0.500 U/ml. A strong relationship was observed between virulence of the isolates and cuticle degrading enzyme production as increased enzyme production was observed for virulent isolates. Conclusion: In the present study three isolates as (MIS2, MIS7 and MIS13) demonstrated cuticle degrading enzyme (CDE) that indicate higher virulence based on the bioassay conducted earlier by the authors as strongly substantiating the role of CDEs is considered the virulence of Metarhizium isolates. So, these isolates may be as ecofriendly insect-pest control agent in future. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17648 J. bio-sci. 20: 25-32, 2012

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