Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog

2010 ◽  
Vol 12 (2) ◽  
Author(s):  
Ernie Hernawati Purwaningsih ◽  
Nurhadi Ibrahim ◽  
Hamdani Zain ◽  
Arjo Tedjo
Keyword(s):  
Ex Vivo ◽  
Water Extract ◽  
Musculus Gastrocnemius ◽  
Neuro Protection ◽  
Acalypha Indica

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

2008 ◽  
Vol 116 (3) ◽  
pp. 439-446 ◽  
Author(s):  
Pilaipark Chumark ◽  
Panya Khunawat ◽  
Yupin Sanvarinda ◽  
Srichan Phornchirasilp ◽  
Noppawan Phumala Morales ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Moringa Oleifera Lam

scholarly journals Comprehensive Chemical Profiling and Multidirectional Biological Investigation of Two Wild Anthemis Species (Anthemis tinctoria var. Pallida and A. cretica subsp. tenuiloba): Focus on Neuroprotective Effects

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2582 ◽  
Author(s):  
Giustino Orlando ◽  
Gokhan Zengin ◽  
Claudio Ferrante ◽  
Maurizio Ronci ◽  
Lucia Recinella ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Physiological Activity ◽  
Pharmacological Study ◽  
Water Extract ◽  
Aqueous Extracts ◽  
Neuroprotective Effects ◽  
Biological Investigation ◽  
Aerial Parts ◽  
Key Enzyme ◽  
Specific Proteins

Ethyl acetate (EA), methanol (MeOH), and aqueous extracts of aerial parts of Anthemis tinctoria var. pallida (ATP) and A. cretica subsp. tenuiloba (ACT) were investigated for their phenol and flavonoid content, antioxidant, and key enzyme inhibitory potentials. All extracts displayed antiradical effects, with MeOH and aqueous extracts being a superior source of antioxidants. On the other hand, EA and MeOH extracts were potent against AChE and BChE. Enzyme inhibitory effects against tyrosinase and α-glucosidase were observed, as well. We also studied Anthemis extracts in an ex vivo experimental neurotoxicity paradigm. We assayed extract influence on oxidative stress and neurotransmission biomarkers, including lactate dehydrogenase (LDH) and serotonin (5-HT), in isolated rat cortex challenged with K+ 60 mM Krebs-Ringer buffer (excitotoxicity stimulus). An untargeted proteomic analysis was finally performed in order to explore the putative mechanism in the brain. The pharmacological study highlighted the capability of ACT water extract to blunt K+ 60 mM increase in LDH level and 5-HT turnover, and restore physiological activity of specific proteins involved in neuron morphology and neurotransmission, including NEFMs, VAMP-2, and PKCγ, thus further supporting the neuroprotective role of ACT water extract.

scholarly journals Water Extract from Inflorescences of Industrial Hemp Futura 75 Variety as a Source of Anti-Inflammatory, Anti-Proliferative and Antimycotic Agents: Results from In Silico, In Vitro and Ex Vivo Studies

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 437 ◽  
Author(s):  
Giustino Orlando ◽  
Lucia Recinella ◽  
Annalisa Chiavaroli ◽  
Luigi Brunetti ◽  
Sheila Leone ◽  
...  
Keyword(s):  
In Silico ◽  
Ex Vivo ◽  
Hct116 Cell ◽  
Water Extract ◽  
Network Pharmacology ◽  
Rat Colon ◽  
Microsporum Gypseum ◽  
In Silico Studies ◽  
Industrial Hemp

Industrial hemp (Cannabis sativa) is traditionally cultivated as a valuable source of fibers and nutrients. Multiple studies also demonstrated antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences. On the other side, only a few studies explored the potential pharmacological application of polar extracts from inflorescences. In the present study, we investigated the water extract from inflorescences of industrial hemp Futura 75 variety, from phytochemical and pharmacological point of view. The water extract was assayed for phenolic compound content, radical scavenger/reducing, chelating and anti-tyrosinase effects. Through an ex vivo model of toxicity induced by lipopolysaccharide (LPS) on isolated rat colon and liver, we explored the extract effects on serotonin, dopamine and kynurenine pathways and the production of prostaglandin (PG)E2. Anti-proliferative effects were also evaluated against human colon cancer HCT116 cell line. Additionally, antimycotic effects were investigated against Trichophyton rubrum, Trichophyton interdigitale, Microsporum gypseum. Finally, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted in order to predict the putative targets underlying the observed pharmacological and microbiological effects. Futura 75 water extract was able to blunt LPS-induced reduction of serotonin and increase of dopamine and kynurenine turnover, in rat colon. Additionally, the reduction of PGE2 levels was observed in both colon and liver specimens, as well. The extract inhibited the HCT116 cell viability, the growth of T. rubrum and T. interdigitale and the activity of tyrosinase, in vitro, whereas in silico studies highlighting the inhibitions of cyclooxygenase-1 (induced by carvacrol), carbonic anhydrase IX (induced by chlorogenic acid and gallic acid) and lanosterol 14-α-demethylase (induced by rutin) further support the observed pharmacological and antimycotic effects. The present findings suggest female inflorescences from industrial hemp as high quality by-products, thus representing promising sources of nutraceuticals and cosmeceuticals against inflammatory and infectious diseases.

Morinda citrifolianoni water extract enhances innate and adaptive immune responses in healthy mice, ex vivo, and in vitro

2019 ◽  
Vol 33 (3) ◽  
pp. 676-689 ◽  
Author(s):  
Yo Han Hong ◽  
Young-Su Yi ◽  
Sang Yun Han ◽  
Nur Aziz ◽  
Han Gyung Kim ◽  
...  
Keyword(s):  
Immune Responses ◽  
Ex Vivo ◽  
Water Extract ◽  
Adaptive Immune Responses ◽  
Adaptive Immune

Gastroprotective Effect of Fructus Evodiae Water Extract on Ethanol-Induced Gastric Lesions in Rats

2006 ◽  
Vol 34 (06) ◽  
pp. 1027-1035 ◽  
Author(s):  
Xiao Yu ◽  
Da-Zheng Wu ◽  
Jian-Ye Yuan ◽  
Rong-Rong Zhang ◽  
Zhi-Bi Hu
Keyword(s):  
Gastric Lesion ◽  
Ex Vivo ◽  
Water Extract ◽  
Mucosal Barrier ◽  
Dependent Manner ◽  
Gastric Lesions ◽  
Gastric Mucosal Lesions ◽  
Nitrate And Nitrite ◽  
Dose Dependent ◽  
Lesion Index

Fructus Evodiae is a widely used herbal medicine with anti-inflammatory and analgetic activities in China. The present study was designed to investigate the effect of Fructus Evodiae water extract (FE) on ethanol-induced gastric lesions in rats. Three hours before ethanol challenge, animals were intraperitoneally treated with FE (424.8 mg/kg, 141.6 mg/kg, and 47.6 mg/kg). Subsequently, we employed ex-vivo chamber technique to examine the effect of FE on gastric transmucosal potential difference (PD) changes. NOx (nitrate and nitrite) in gastric perfusate and gastric lesion index of whole glandular stomach were determined by intubation. The results showed that FE dose-dependently accelerated the recovery of PD reduction by ethanol, and increased NOx production in gastric perfusate. FE also inhibited gastric lesion formation in a dose-dependent manner. These results suggested that FE prevented ethanol-induced gastric mucosal lesions by strengthening the mucosal barrier integrity and increasing gastric mucosal nitric oxide (NO) synthesis.

scholarly journals Nanotechnology in Neurology—Current Status and Future Possibilities

US Neurology ◽  
2010 ◽  
Vol 06 (01) ◽  
pp. 12 ◽  
Author(s):  
James M Provenzale ◽  
Aaron M Mohs ◽  
◽  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Ex Vivo ◽  
Current Status ◽  
Medical Applications ◽  
Nanoparticle Delivery ◽  
New Methods ◽  
The Central Nervous System ◽  
Neuro Protection

The field of nanomedicine is rapidly emerging and will provide many novel methods for diagnosis and treatment. In this article the applications of nanotechnology to the central nervous system (CNS) will be described. Nanotechnology provides many potential solutions to various problems encountered in CNS diseases. Specifically, nanomedicine offers the possibility of new methods of drug delivery, more sensitive and specific means for diagnosis of disease at earlier stages and assessment of treatment response, and also potential techniques for neuro-protection and neuro-engineering. In this article, information is provided on the various types of nanoparticles involved in medical applications, the principles of nanoparticle delivery and targeting, and bothin vivoandex vivouses of nanoscale materials.

scholarly journals Edible Bird’s Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte

2021 ◽  
Vol 12 ◽  
Author(s):  
Queenie Wing Sze Lai ◽  
Maggie Sui Sui Guo ◽  
Kevin Qiyun Wu ◽  
Zhitao Liao ◽  
Dongshi Guan ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Mapk Pathway ◽  
Control Cell ◽  
Water Extract ◽  
Skin Barrier Function ◽  
Health Food ◽  
Cultured Keratinocytes ◽  
Edible Bird’S Nest ◽  
Skin Function

Edible bird’s nest (EBN) has been consumed as a Chinese delicacy for hundreds of years; the functions of which have been proposed to prevent lung disease, strengthen immune response, and restore skin youthfulness. To support the skin function of EBN, the water extract and the enzymatic digest of EBN with enriched digested peptides were tested in cultured keratinocyte, HaCaT cell line. The effects of EBN extract and digest in inducing proteins crucial for skin moisturizing were determined in both in vitro and ex vivo models. In cultured keratinocytes, the expressions of S100-fused type proteins contributing to skin barrier function in the stratum corneum, e.g. filaggrin and filaggrin-2, were determined in both mRNA and protein levels, which were markedly induced in the treatment of EBN extract or digest. The EBN-induced gene transcriptions of filaggrin and filaggrin-2 were mediated by activation of p38 MAPK pathway and various transcription factors, e.g. GATA3, PPARα, PPARβ, and PPARγ: these transcriptional factors were markedly activated by the digested products of EBN, as compared to the extract, in cultured keratinocytes. By using atomic force microscopy (AFM), the EBN-treated keratinocyte was shown to have more liquid-like morphology, as compared to a control cell. The EBN digest showed better induction on these moisturizing effects as compared to the extract. These lines of evidence therefore suggested the water moisturizing effect of EBN in skin function.

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