In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

2021 ◽  
Vol 9 (1) ◽  
pp. 4
Author(s):  
Paria Motahari ◽  
Hossein Samadi Kafil ◽  
Zahra Taheri Bonab
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Antimicrobial Agents ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Inhibitory Effect ◽  
New Drugs ◽  
Colony Count ◽  
Significant Difference

Antimicrobial agents are used as intra-canal drugs or canal cleaners for complete dental root canal cleaning. This new study attempts to compare the antibacterial effect of 2% chlorhexidine and combination of honey and Aloe vera extract (H-Av mixture) against Enterococcus faecalis (E.faecalis) and Escherichia coli (E.coli) which are the most important pathogens isolated from infectious root canals. Enterococcus faecalis (ATCC® 29212™) and Escherichia coli (ATCC® 25922™) were cultured in the tryptic soy broth medium. Dilution of H-Av mixture by the method of macrodilution to determine Minimum Inhibitory Concentration was investigated. In this paper agar well diffusion and colony count methods were also used to ensure the accuracy of the results. The results were statistically analyzed by student’s t-test. The significant level established at 5% (P<0.05). The results of the methods of macrodilution, colony count and Agar well diffusion confirm the inhibitory effect of H-Av mixture on E.coli and E. fecalis. Statistically, there was no significant difference between the antibacterial effect of 2% chlorhexidine and 25% H-Av mixture (P>0.05) Increasing drug resistance to antimicrobial compounds needs to study of new drugs against pathogens. H-Av mixture with benefits such as availability, good taste and easier use than chlorhexidine, and fewer side effects can be a good option for intracanal irrigation after clinical trials.

scholarly journals Antibacterial Properties of Aloe vera on Intracanal Medicaments against Enterococcus faecalis Biofilm at Different Stages of Development

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Negin Ghasemi ◽  
Mahsa Behnezhad ◽  
Mohammad Asgharzadeh ◽  
Elham Zeinalzadeh ◽  
Hossein Samadi Kafil
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Study Groups ◽  
Single Root ◽  
Significant Difference ◽  
Planktonic Form ◽  
Phosphate Buffered Saline

Background. Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the Aloe vera compound whose antibacterial effect has already been proven in the planktonic form of Enterococcus faecalis. The purpose of this study is to evaluate the antibacterial effect of Aloe vera on E. faecalis biofilms at the 4th and 6th week of development. Materials and Methods. 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with Aloe vera, calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining E. faecalis counts. Results. The CFU mean in the 4th week subgroup in Aloe vera, phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6th week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials ( p < 0.05 ). Conclusion. Aloe vera, in contrast to calcium hydroxide, eliminated 4th and 6th week biofilms and showed remarkable antibacterial properties against E. faecalis biofilm. These results support potency of Aloe vera to use as a natural antimicrobial material in the intracanal medicament.

scholarly journals Antibacterial Effect of Autologous Platelet-Rich Gel Derived from Subjects with Diabetic Dermal Ulcers In Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Lihong Chen ◽  
Chun Wang ◽  
Hengchuan Liu ◽  
Guanjian Liu ◽  
Xingwu Ran
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Effect ◽  
Control Group ◽  
Diabetic Patients ◽  
Antibacterial Effects ◽  
Significant Difference ◽  
Autologous Platelet

Background. Autologous platelet-rich gel (APG) is an effective method to improve ulcer healing. However, the mechanisms are not clear. This study aimed to investigate the antibacterial effect of APG in vitro.Methods. Platelet-rich plasma (PRP), platelet-poor plasma (PPP) and APG were prepared from whole blood of sixteen diabetic patients with dermal ulcers. Antibacterial effects againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawere evaluated by bacteriostasis assay of APG, PRP, and APG-APO (APG combined with apocynin), with phosphate-buffered saline (PBS) and PPP as the control group.Results. (1) Compared to the PBS and PPP, the APG and APG-APO groups showed strong antibacterial activity againstStaphylococcus aureus. There was no significant difference(P>0.05)between APG and APG-APO. (2) Compared to PBS, APG, APG-APO, and PRP showed obvious antibacterial effects againstEscherichia coliandPseudomonas aeruginosa. No significant difference(P>0.05)was revealed among the three groups. Compared to the PPP group, they did not show antibacterial effect againstEscherichia coliandPseudomonas aeruginosa(P>0.05).Conclusions. APG has antibacterial effect againstStaphylococcus aureusmediated by platelet activation in the diabetic patients with dermal ulcer, and does not present obvious antibacterial effect againstEscherichia coliorPseudomonas aeruginosa. Combination of APG and antibiotics may have synergistic antibacterial effect.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

Serological diversity, molecular characterisation and antimicrobial sensitivity of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in Kashmir, India

2019 ◽  
Vol 59 (2) ◽  
pp. 338
Author(s):  
S. N. Magray ◽  
S. A. Wani ◽  
Z. A. Kashoo ◽  
M. A. Bhat ◽  
S. Adil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Significant Difference ◽  
Virulence Gene Profile

The present study has determined the serological diversity, virulence-gene profile and in vitro antibiogram of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in India suspected to have died of colibacillosis. The virulence-gene profile of APEC was compared with that of the Escherichia coli isolates from faeces of apparently healthy chickens, called avian faecal E. coli (AFEC). In total, 90 representative isolates of APEC and 63 isolates of AFEC were investigated in the present study. The APEC were typed into 19 serogroups, while some isolates were rough and could not be typed. Most prevalent serogroup was O2 (24.44%). Among the eight virulence genes studied, the prevalence of seven genes (iss, iucD, tsh, cva/cvi, irp2, papC and vat) was significantly higher in APEC than in AFEC isolates. However, there was no significant difference between APEC and AFEC isolates for possession of astA gene. The most frequent gene detected among the two groups of organisms was iss, which was present in 98.88% and 44.44% of APEC and AFEC isolates respectively. The in vitro antibiogram showed that the majority (96.6%) of APEC isolates were resistant to tetracycline, while 82.2% were resistant to cephalexin, 78.8% to cotrimoxazole, 68.8% to streptomycin and 63.3% to ampicillin. However, most of them (84.45%) were sensitive to gentamicin. Thus, it is concluded that APEC from the broiler chickens carried putative virulence genes that attributed to their pathogenicity. Furthermore, the majority of APEC isolates were found to be multi-drug resistant, which, in addition to leading treatment failures in poultry, poses a public health threat.

Evaluation of the antibacterial effect of tea tree oil on Enterococcus faecalis and biofilm in vitro

2021 ◽  
pp. 114566
Author(s):  
Jianyan Qi ◽  
Min Gong ◽  
Rui Zhang ◽  
Yumeng Song ◽  
Qian Liu ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Tea Tree Oil ◽  
Tea Tree

Immunomodulatory activities of whey fractions in efferent prefemoral lymph of sheep

1996 ◽  
Vol 63 (2) ◽  
pp. 257-267 ◽  
Author(s):  
Chun W. Wong ◽  
Geoffrey O. Regester ◽  
Geoffrey L. Francis ◽  
Dennis L. Watson
Keyword(s):  
Immune Responses ◽  
Bovine Milk ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Milk Whey ◽  
Significant Difference ◽  
Lymphocyte Phenotypes ◽  
Dose Dependent

SummaryStudies on the immunomodulatory activities of ruminant milk and colostral whey fractions were undertaken. By comparing with boiled colostral whey in a preliminary experiment, a putative heat-labile immunostimulatory factor for antibody responses was found to be present in ovine colostral whey. Studies were then undertaken in sheep in which the efferent prefemoral lymphatic ducts were cannulated bilaterally, and immune responses in the node were measured following subcutaneous injection in the flank fold of whey protein preparations of various purities. A significant sustained decline of efferent lymphocyte output was observed following injection with autologous crude milk whey or colostral whey preparations, but no changes were observed in interferon-gamma levels in lymph plasma. Two bovine milk whey fractions (lactoperoxidase and lactoferrin) of high purity were compared in bilaterally cannulated sheep. A transient decline over the first 6 h was seen in the efferent lymphocyte output and lymph flow rate after injection of both fractions. A significant difference was seen between the two fractions in interferongamma levels in lymph at 6 h after injection. However, no significant changes in the proportion of the various efferent lymphocyte phenotypes were seen following either treatment. Whereas both fractions showed a significant inhibitory effect in a dose-dependent manner on the proliferative response of T lymphocytes, but not B lymphocytes, to mitogenic stimulation in vitro, no similar changes were seen following in vivo stimulation with these two fractions.

Antibacterial Effect of Two Mineral Trioxide Aggregate (MTA) Preparations Against Enterococcus faecalis and Streptococcus sanguis In Vitro

2006 ◽  
Vol 32 (11) ◽  
pp. 1053-1056 ◽  
Author(s):  
Khalid Al-Hezaimi ◽  
Thakib A. Al-Shalan ◽  
Jafar Naghshbandi ◽  
Samuel Oglesby ◽  
James H.S. Simon ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Mineral Trioxide Aggregate ◽  
Streptococcus Sanguis

scholarly journals The Effect of Antimicrobial Drugs Tylocolinum, Tetragold and Cidisept-o on Escherichia coli Ultrastructure

2013 ◽  
Vol 2 (3) ◽  
pp. 65
Author(s):  
A. G. Shakhov ◽  
D. V. Fedosov ◽  
L. Y. Sashnina ◽  
O. V. Kazimirov
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Water Solution ◽  
Uranyl Acetate ◽  
Chemotherapeutic Agents ◽  
Control Culture ◽  
New Drugs ◽  
Ultrastructural Changes ◽  
The Impact

<p>As a result of wide antibiotics, sulfonamides and other antimicrobial agents usage for the therapy of the animals with the bacterial infections caused by various causative agents including <em>Escherichia coli</em>, many microorganisms gained resistance to the chemotherapeutic agents. New combined drugs are being worked out during recent years, the components of which have various influence mechanisms on the bacterial cell that helps to provide resistance forming control. The results of the researches of the new antimicrobial agents, containing antibiotics in their composition, and non-antibiotic agent influence on the ultrastructure of <em>Escherichia coli</em> are represented in this study.</p> <p>5-hour <em>Escherichia coli 866</em> culture was processed by the drugs of the minimum bactericidal (Tylocolinum-0.39 µg/ml, Tetragold-6.25 µg/ml, Cidisept-o-25 µg/ml) and 4-time concentrations during 3 hours. Samples and control culture (without drugs) were fixed by the 2.5% glutaricdialdehyde on the s-Collidine Buffer, dehydrated in the ethanol with rising concentration, filled in epoxies. Ultrathin slices were stained by 2% water solution of uranyl acetate and lead citrate for 10 minutes. Then they were examined with the use of the electron microscope JEM-100 CX II by JEOL.</p> <p>The research showed deep ultrastructural changes in <em>Escherichia coli</em> cells under the antimicrobial agent influence determined by synergistic effect of combined Tylocolinum and Tetragold drugs components, possessing various bacteria influencing mechanisms, and aldehyde that is a component of Cidisept-o.</p> The electron microscopy usage allows to get unique information about the impact consequences of the traditional improved drugs and new drugs with antimicrobial activity on the bacterial infectious agents.

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